• Parasites, Hosts and Diseases
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• v.47 no.2
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• pp.103-107
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• 2009

The genus Acanthamoeba can cause severe infections such as granulomatous amebic encephalitis and amebic keratitis in humans. However, little genomic information of Acanthamoeba has been reported. Here, we constructed Acanthamoeba expressed sequence tags (EST) database (Acanthamoeba EST DB) derived from our 4 kinds of Acanthamoeba cDNA library. The Acanthamoeba EST DB contains 3,897 EST generated from amebae under various conditions of long term in vitro culture, mouse brain passage, or encystation, and downloaded data of Acanthamoeba from National Center for Biotechnology Information (NCBI) and Taxonomically Broad EST Database (TBestDB). The almost reported eDNA/genomic sequences of Acanthamoeba provide stand alone BLAST system with nucleotide (BLAST NT) and amino acid (BLAST AA) sequence database. In BLAST results, each gene links for the significant information including sequence data, gene orthology annotations, relevant references, and a BlastX result. This is the first attempt for construction of Acanthamoeba database with genes expressed in diverse conditions. These data were integrated into a database (http://www. amoeba.or.kr).

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.4
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• pp.458-468
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• 2007

To tolerate environmentally adverse conditions such as cold, drought, and salinity, plants often synthesize and accumulate proline in cells as compatible osmolytes. ${\Delta}^1$-Pyrroline-5-carboxylate synthetase(P5CS) catalyzes the rate-limiting step of proline biosynthesis from glutamate. Two complete genes, MtP5CS1 and MtP5CS2, were isolated from the model legume Medicago truncatula by cDNA cloning and bacterial artificial chromosome library screening. Nucleotide sequence analysis showed that both genes consisted of 20 exons and 19 introns. Alignment of the predicted amino acid sequences revealed high similarities with P5CS proteins from other plant species. The two MtP5CS genes were expressed in response to high salt and low temperature treatments. Semi-quantitative reverse transcription-polymerase chain reaction showed that MtP5CS1 was expressed earlier than MtP5CS2, indicating differential regulation of the two genes. To evaluate the reverse genetic effects of nucleotide changes on MtP5CS function, a Targeting Induced Local Lesions in Genomes approach was taken. Three mutants each were isolated for MtP5CS1 and MtP5CS2, of which a P5CS2 nonsense mutant carrying a codon change from arginine to stop was expected to bring translation to premature termination. These provide a valuable genetic resource with which to determine the function of the P5CS genes in environmental stress responses of legume crops.

• Journal of Microbiology and Biotechnology
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• v.15 no.3
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• pp.525-531
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• 2005

From a genomic library of Lactobacillus reuteri ATCC 55739, one clone, NE347, carrying a pyrH gene encoding UMP kinase, was identified. pNE347 carried a 1.88 kb EcoRI fragment and the pyrH was located in the middle of the insert. pyrH ORF was 723 bp in size and capable of encoding UMP kinase composed of 240 amino acid residues. tsf encoding an elongation factor-Ts and frr encoding a ribosomal recycling factor were present upstream and downstream of pyrH, respectively. When introduced into E. coli KUR1244, a pyrH-negative strain, pNE347 restored the ability to grow at $42^{\circ}C$, indicating that pyrH from L. reuteri synthesized functional UMP kinase in E. coli. Northern blot experiment showed that pyrH and frr were cotranscribed as a 1.4 kb single transcript. pyrH was overexpressed in E. coli by using a pET26b(+) vector, and a major 25 kDa protein band appeared on SDS-polyacrylamide gel.

• Journal of Radiation Protection and Research
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• v.14 no.1
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• pp.56-65
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• 1989

The nuclear criticality of the KSC-4 shipping cask which can load four assemblies of PWR spent fuel was analyzed using KENO-IV computer code and 19-group nuclear cross section set generated from 218-group neutron cross section library(DLC-43/CSRL) using AMPX system. In accordance with 10CFR71, the analysis was performed for fresh fuel assemblies, instead of the spent fuels, under both norml transportation and hypothetical accident conditions. The calculated maximum multiplication factors(Keff) of the KSC-4 cask were 0.85289 and 0.94185 for the normal transportation and hypothetical accident conditions, respectively. The highest Keff of the KSC-4 cask is within the subcritical limit prescribed in l0CFR71 and accordingly the KSC-4 cask is safely designed in terms of nulear criticality.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.30 no.5C
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• pp.371-381
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• 2005

In this paper, we propose an advanced hardware architecture for the CAVLC entropy encoder/decoder engine for real time video compression. The CAVLC (Context-based Adaptive Variable Length Coding) is a lossless compression method in H.264/AVC and it has high compression efficiency but has computational complexity. The reference memory size is optimized using partitioned storing method and memory reuse method which are based on partiality of memory referencing. We choose the hardware architecture which has the most suitable one in several encoder/decoder architectures for the mobile devices and improve its performance using parallel processing. The proposed architecture has been verified by ARM-interfaced emulation board using Altera Excalibur and also synthesized on Samsung 0.18 um CMOS technology. The synthesis result shows that the encoder can process about 300 CIF frames/s at 150MHz and the decoder can process about 250 CIF frames/s at 140Mhz. The hardware architectures are being used as core modules when implementing a complete H.264/AVC video encoder/decoder chip for real-time multimedia application.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.11C
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• pp.1029-1039
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• 2009

In this paper, the existing disparity aquisition algorithms were analyzed, on the bases of which a disparity generation technique that is superior in accuracy to the generation time was proposed. Basically it uses a pixel-by-pixel motion estimation technique. It has a merit of possibility of a high-speed operation. But the motion estimation technique has a disadvantage of lower accuracy because it depends on the similarity of the matching window regardless of the distribution characteristics of the texture in an image. Therefore, an enhanced technique to increase the accuracy of the disparity is required. This paper introduced a variable-sized window matching technique for this requirement. By the proposed technique, high accuracies could be obtained at the homogeneous regions and the object edges. A hardware to generate disparity image was designed, which was optimized to the processing speed so that a high throughput is possible. The hardware was designed by Verilog-HDL and synthesized using Hynix $0.35{\mu}m$ CMOS cell library. The designed hardware was operated stably at 120MHz using Cadence NC-VerilogTM and could process 15 frames per second at this clock frequency.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.11C
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• pp.940-948
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• 2008

This paper presents a low-power, low-complexity design and implementation results of a high speed multiple-input multiple-output orthogonal frequency division multiplexing (MIMO-OFDM) wireless LAN (WLAN) baseband processor. The proposed processor is composed of the physical layer convergence procedure (PLCP) processor and physical medium dependent (PMD) processor, which have been optimized to have low-power and reduced-complexity architecture. It was designed in a hardware description language (HDL) and synthesized to gate-level circuits using 0.18um CMOS standard cell library. As a result, the proposed TX-PLCP processor reduced the power consumption by as much as 81% over the bit-level operation architecture. Also, the proposed MIMO symbol detector reduced the hardware complexity by 18% over the conventional SQRD-based architecture with division circuits and square root operations.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.6C
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• pp.450-458
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• 2008

In this paper, we propose a real-time stereoscopic display system based on H.264/AVC. We initially acquire stereo-view images from stereo web-cam using OpenCV library. The captured images are converted to YUV 4:2:0 format as a preprocess. The input files are encoded by stereo-encoder, which has a proposed estimation structure, with more than 30 fps. The encoded bitstream are decoded by stereo-decoder reconstructing left and right images. The reconstructed stereo images are postprocessed by stereoscopic image synthesis technique to offer users more realistic images with 3D effect. Experimental results show that the proposed system has better encoding efficiency compared with using a conventional stereo CODEC(coder and decoder) and operates with real-time processing and low complexity suitable for an application with a mobile environment.

• 한국균학회소식:학술대회논문집
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• 2015.11a
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• pp.35-35
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• 2015

Pleurotus eryngii has recently become a major cultivated mushroom; it uses tetrapolar heterothallism as a part of its reproductive process. Sexual development progresses only when the A and B mating types are compatible. Such mating incompatibility occasionally limits the efficiency of breeding programs in which crossing within loci-shared strains or backcrossing strategies are employed. Therefore, understanding the mating system in edible mushroom fungi will help provide a short cut in the development of new strains. We isolated and identified pheromone and receptor genes in the B3 locus of P. eryngii and performed a functional analysis of the genes in the mating process by transformation. A genomic DNA library was constructed to map the entire mating-type locus. The B3 locus was found to contain four pheromone precursor genes and four receptor genes. Remarkably, receptor PESTE3.3.1 has just 34 amino acid residues in its C-terminal cytoplasmic region; therefore, it seems likely to be a receptor-like gene. Real-time quantitative RT-PCR (real-time qRT-PCR) revealed that most pheromone and receptor genes showed significantly higher expression in monokaryotic cells than dikaryotic cells. The pheromone genes PEphb3.1 and PEphb3.3 and the receptor gene PESTE3.3.1 were transformed into P5 (A3B4). The transformants were mated with a tester strain (A4B4), and the progeny showed clamp connections and a normal fruiting body, which indicates the proposed role of these genes in mating and fruiting processes. This result also confirms that PESTE3.3.1 is a receptor gene. In this study, we identified pheromone and receptor genes in the B3 locus of P. eryngii and found that some of those genes appear to play a role in the mating and fruiting processes. These results might help elucidate the mechanism of fruiting differentiation and improve breeding efficiency.

• Journal of Korean Society on Water Environment
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• v.24 no.1
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• pp.19-29
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• 2008

Hot air sparging is a groundwater remediation technique, in which organic contaminants volatilized into hot air from the saturated to vadose zone. In the laboratory diesel (10,000 mg TPH/kg) was spiked in contaminated saturated aquifer soil. The hot air ($34.9{\pm}2.7^{\circ}C$) was injected in intermittent (Q=1,500 mL/min, 10 minute injection and 10 minute idle) modes. We performed microcosm tests using the groundwater samples to assess TPH reductive remediation activity. For Terminal-Restriction Fragment Length Polymorphism (T-RFLP) analysis of eubacterial communities in sludge of wastewater treatment plants and soil of experiment site, the 16S rDNA was amplified by Polymerase Chain Reaction (PCR) from the sludge and the soil. The obtained 16S rDNA fragments were digested with Msp I and separated by electrophoresis gel. We found various sequence types for hot air sparging experiment with sludge soil samples that were closely related to Bacillus (149 bp, Firmicutes), Methlobacterium (149 bp, Euryarchaeotes), Pseudomonas (492 bp, ${\gamma}$-Proteobacteria), etc., in the clone library. In this study we find that TPH-water was reduced to 78.9% of the initial value in this experiment aquifer. The results of the present study suggests that T-RFLP method may be applied as a useful tool for the monitoring in the TPH contaminated soil fate of microorganisms in natural microbial community.