• Title/Summary/Keyword: C : N : P ratio

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Weight Loss and Nutrient Dynamics during Leaf Litter Decomposition of Quercus mongolica in Mt. Worak National Park

  • Mun, Hyeong-Tae
    • Journal of Ecology and Environment
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    • v.32 no.2
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    • pp.123-127
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    • 2009
  • Weight loss and nutrient dynamics of Quercus mongolica leaf litter during decomposition were investigated from December 2005 through August 2008 in Mt. Worak National Park as a part of National Long-Term Ecological Research Program in Korea. The decay constant (k) of Q. mongolica litter was 0.26. After 33 months decomposition, remaining weight of Q. mongolica litter was 49.3$\pm$4.4%. Initial C/N and C/P ratios of Q. mongolica litter were 43.3 and 2,032, respectively. C/N ratio in decomposing litter decreased rapidly from the beginning to nine months decomposition, and then showed more or less constant. C/P ratio increased to 2,407 after three months decomposition, and then decreased steadily thereafter. N and P concentration increased significantly during decomposition. N immobilization occurred from the beginning through 18 months decomposition, and mineralization occurred afterwards in decomposing litter. P immobilized significantly from fifteen months during decomposition. K concentration decreased rapidly from the beginning to six months decomposition. However it showed an increasing pattern during later stage of decomposition. Remaining K decreased rapidly during early stage of decomposition. There was no net K immobilization. Ca concentration increased from the beginning to twelve months decomposition, and then decreased rapidly till twenty one months elapsed. However, it increased again thereafter. Ca mineralization occurred from fifteen months. Mg concentration increased during decomposition. There was no Mg immobilization during litter decomposition. After 33 months decomposition, remaining N, P, K, Ca and Mg in Q. mongolica litter were 79.2, 110.9, 36.2, 52.7 and 74.4%, respectively.

Effects of dietary n-6:n-3 polyunsaturated fatty acid ratio on growth performance, blood indexes, tissue fatty acid composition and the expression of peroxisome proliferator-activated receptor gamma signaling related genes in finishing pigs

  • Chen, Jing;Cui, Hongze;Liu, Xianjun;Li, Jiantao;Zheng, Jiaxing;Li, Xin;Wang, Liyan
    • Animal Bioscience
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    • v.35 no.5
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    • pp.730-739
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    • 2022
  • Objective: This study investigated the effects of dietary n-6:n-3 polyunsaturated fatty acid (PUFA) ratio on growth performance, blood indexes, tissue fatty acid composition and the gene expression in finishing pigs. Methods: Seventy-two crossbred ([Duroc×Landrace]×Yorkshire) barrows (68.5±1.8 kg) were fed one of four isoenergetic and isonitrogenous diets with n-6:n-3 PUFA ratios of 2:1, 3:1, 5:1, and 8:1. Results: Average daily gain, average daily feed intake and gain-to-feed ratio had quadratic responses but the measurements were increased and then decreased (quadratic, p<0.05). The concentrations of serum triglyceride, total cholesterol and interleukin 6 were linearly increased (p<0.05) with increasing of dietary n-6:n-3 PUFA ratio, while that of high-density lipoprotein cholesterol tended to decrease (p = 0.062), and high-density lipoprotein cholesterol:low-density lipoprotein cholesterol ratio and leptin concentration were linearly decreased (p<0.05). The concentration of serum adiponectin had a quadratic response but the measurement was decreased and then increased (quadratic, p<0.05). The proportion of C18:3n-3 was linearly decreased (p<0.05) in the longissimus thoracis (LT) and subcutaneous adipose tissue (SCAT) as dietary n-6:n-3 PUFA ratio increasing, while the proportion of C18:2n-6 and n-6:n-3 PUFA ratio were linearly increased (p<0.05). In addition, the expression levels of peroxisome proliferator-activated receptor gamma (PPARγ) and lipoprotein lipase in the LT and SCAT, and adipocyte fatty acid binding protein and hormone-sensitive lipase (HSL) in the SCAT had quadratic responses but the measurements were increased and then decreased (quadratic, p<0.05). The expression of HSL in the LT was linearly decreased (p<0.05) with increasing of dietary n-6:n-3 PUFA ratio. Conclusion: Dietary n-6:n-3 PUFA ratio could regulate lipid and fatty acid metabolism in blood and tissue. Reducing dietary n-6:n-3 PUFA ratio (3:1) could appropriately suppress expression of related genes in PPARγ signaling, and result in improved growth performance and n-3 PUFA deposition in muscle and adipose tissue in finishing pigs.

Short-term Nutrient Enrichment Bioassays (NEBs) by Manipulation of TN:TP Ratios and the Response of Primary Productivity (as Chlorophyll-a) (N:P Ratio 조절에 의한 단기 영양염 첨가 바이오에세이(NEBs) 및 1차 생산력(엽록소-a)의 반응성 테스트)

  • Jeong, Da-Bin;An, Kwang-Guk
    • Korean Journal of Environmental Biology
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    • v.31 no.4
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    • pp.383-392
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    • 2013
  • The objective of this study was to determine the effects of N:P ratio on primary productivity measured as chlorophyll-a (CHL) using the approach of In Situ Nutrient Enrichment Bioassays (NEBs) in Daechung Reservoir. The effects of NEBs on the N:P mass ratios were compared with the field data obtained from monthly-chemical monitoring during 2009~2012. The short-term NEBs showed that the response of primary productivity in the phosphorus spiked treatments (5, 15, 20 and 30 N:P ratios) were greater than the responses in the control (C) and nitrogen spiked treatment (N:P ratio=150, $T_{VI}$). The response in the nitrogen treatment (N:P ratio=150, $T_{VI}$) was less compared to control and all five treatments ($T_I{\sim}T_{VI}$). The outcomes of the NEBs suggest that phosphorus limited the phytoplankton growth and nitrogen addition inhibited the algal growth. In the analysis of nutrients and CHL from the ambient epilimnetic water in Daechung Reservoir, minimum N:P ratios resulted in maximum concentrations of CHL. Overall, our results suggest that the N:P ratio was the key factor in regulating the phytoplankton growth in NEB experiments.

Effects of dietary fat saturation level on growth performance, carcass traits, blood lipid parameters, tissue fatty acid composition and meat quality of finishing pigs

  • Chen, Jing;Li, Jiantao;Liu, Xianjun;He, Yang
    • Animal Bioscience
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    • v.34 no.5
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    • pp.895-903
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    • 2021
  • Objective: The objective of this study was to investigate the effects of various dietary unsaturated to saturated fatty acids ratios (UFA to SFA ratios) on growth performance, carcass traits, blood lipid parameters, tissue fatty acid (FA) composition, and meat quality of finishing pigs. Methods: A total of 45 crossbred pigs ([Duroc×Landrace]×Yorkshire), with an average initial body weight of 60.3±2.4 kg, were randomly allocated to three treatment groups of 1:1, 2:1, and 3:1 dietary UFA to SFA ratios. Results: Both average daily gain and average daily feed intake of pigs were decreased linearly (p<0.05), whereas backfat thickness was decreased linearly (p<0.05) with increasing of dietary UFA to SFA ratio. Serum triglyceride and low density lipoprotein cholesterol were decreased quadratically or linearly (p<0.05) respectively, whereas high density lipoprotein cholesterol was increased quadratically (p<0.05) with increasing dietary UFA to SFA ratio. In M. longissimus thoracis, the proportion of C18:1 and monounsaturated FA was decreased linearly (p<0.05), whereas the proportion of C18:2n-6, C20:4n-6 and polyunsaturated FA (PUFA) were increased linearly (p<0.05) as dietary UFA to SFA ratio increased. In the subcutaneous adipose tissue, the proportion of SFA was decreased linearly (p<0.05), whereas the proportion of n-6 PUFA, n-3 PUFA, and the UFA to SFA ratios were increased linearly (p<0.05) with increasing of dietary UFA to SFA ratio. Meat color scores and shear force of pigs were decreased linearly (p<0.05), whereas drip loss and cooking loss were increased linearly (p<0.05) with increasing of dietary UFA to SFA ratio. Conclusion: Appropriately boosted dietary UFA to SFA ratio could be conductive to optimize blood lipid parameters and tissue FA composition. However, when the ratio is too high or too low it tends to have negative effects on growth performance and meat quality.

Effect of n-6/n-3 fatty acid ratio on metabolic partitioning in hyperlipidemic rats (n-6/n-3 지방산 비율이 고지혈증 랫드의 지질대사 분할에 미치는 영향)

  • Lee, Seunghyung;Um, Kyung-Hwan;Park, Byung-Sung
    • Journal of the Korean Applied Science and Technology
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    • v.35 no.3
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    • pp.643-653
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    • 2018
  • This study was investigated the mechanism of action of n-6/n-3 fatty acid ratio on the metabolic partitioning of blood glycerolipids by in vivo monitoring technique in hyperlipidemic animal model rats. The ratio of cholesteryl 14C-oleate metabolized in the liver of total glycerolipids was lower in the order of n-6/n-3 ratios of 4:1, 15:1, 30:1 and control group (p<0.05). The secretion amount of phospholipid was higher in the order of n-6/n-3 ratio 4:1, 15:1, 30:1 than the control (p<0.05). The secretion amount of triglyceride was lower in especially 4:1, in order of n-6/n-3 4:1, 15:1 and 30:1 compared with the control. The ratio of phospholipid partitioning to total glycerolipid was high in orfer of n-6/n-3 ratio 4:1, 15:1, 30:1 and control (p<0.05). The triacylglycerol partitioning (%) via liver was higher 72.97, 75.93, 78.12% in n-6/n-3 4;1, 15:1, 30:1, respectively than the control of 82.25%, according to increased n-6/n-3 (p<0.05). The phospholipid partitioning (%) was lower 25.15, 18.87, 18.15% in n-6/n-3 4;1, 15:1, 30:1, respectively, compared to control 11.04%, according to increased n-6/n-3 (p<0.05).

Decay Rate and Nutrient Dynamics during Litter Decomposition of Quercus acutissima in Gongju and Jinju (공주와 진주지역에서 상수리나무 낙엽의 분해율 및 분해과정에 따른 영양염류 함량 변화)

  • Won, Ho-Yeon;Oh, Kyung-Hwan;Mun, Hyeong-Tae
    • Journal of Wetlands Research
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    • v.14 no.4
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    • pp.537-545
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    • 2012
  • Decay rate and nutrient dynamics during leaf litter decomposition of deciduous Quercus acutissima were compared between Gongju and Jinju for 33 months from December 2008 through March 2011. Percent remaining weight of Q. acutissima leaf litter after 33 months elapsed in Gongju and in Jinju was $41.2{\pm}0.4%$ and $28.3{\pm}0.6%$, and decay constant (k) was 0.39 and 0.61, respectively. Decomposition in Jinju was significantly faster than that in Gongju. This seemed to be related to higher temperature and precipitation in Jinju than those in Gongju during the experimental period. Initial C/N and C/P ratio of Q. acutissima leaf litter was 46.8 and 270.9, respectively. After 33 months elapsed, C/N and C/P ratios in Gongju decreased to 22.0 and 106.8, and those in Jinju decreased to 19.2 and 170.2, respectively. Initial concentration of N, P, K, Ca and Mg in Q. acutissima leaf litter was 8.31, 0.44, 4.18, 9.38, 1.37 mg/g, respectively. After 33 month elapsed, remaining N, P, K, Ca and Mg were 91.0, 85.4, 30.2, 47.9, 11.7% in Gongju, and 67.0, 54.2, 19.9, 30.0, 40.8% in Jinju, respectively. Except for Mg, remaining nutrients of decomposing leaf litter in Jinju were lower than those in Gongju. In case of N and P, initial immobilization was observed, however, only mineralization was observed in K, Ca and Mg during the whole experimental period.

Interaction of Cu(II)-meso-tetrakis(n-N-methylpyridiniumyl)porphyrin (n = 2,3,4) with Native and Synthetic Polynucleotides Probed by Polarized Spectroscopy

  • Lee, Mi-Jin;Lee, Gil-Jun;Lee, Dong-Jin;Kim, Seog-K.;Kim, Jong-Moon
    • Bulletin of the Korean Chemical Society
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    • v.26 no.11
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    • pp.1728-1734
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    • 2005
  • The interactions of Cu(II)-meso-Tetrakis(n-N-methylpyridiniumyl)porphyrin (n = 2,3,4), respectively referred to as o-, m- and p-CuTMPyP, and DNA, poly$[d(A-T)_2]$ and poly$[d(G-C)_2]$ were investigated by circular and linear dichroism (CD and LD). In the o-CuTMPyP case, in which the rotation of the pyridinium ring is prevented, the shape of the CD spectrum when associated to DNA and poly$[d(A-T)_2]$ resembles and is characterized by a positive band at a low drug to DNA concentration ratio (R ratio) and is bisignate at a high R ratio. The former CD spectrum shape has been attributed to porphyrin that is bound monomerically outside of DNA while the latter can be attributed to those that are stacked. When o-CuTMPyP is bound to poly$[d(G-C)_2]$, the excitonic CD appeared at a relatively high R ratio. In contrast, a characteristic negative CD band in the Soret region was apparent for both m- and p-CuTMPyP when bound to DNA and poly$[d(G-C)_2]$ at the low R ratios, indicating that the porphyrin molecule intercalates. However, the DNA is bent near the intercalation site and the plane of the porphyrin molecule tilts relative to the DNA helix axis, as judged by the magnitude of the reduced LD. Various stacking patterns were identified by the shape of the CD spectrum for m- and p-CuTMPyP when bound to poly$[d(A-T)_2]$. Three species for the former complex and two for the latter complex were found which may reflect the extent of the stacking.

A Study on Optimum Moisture Content and C/N ratio of Sewage Sludge Treatment Using Composting (퇴비화를 이용한 하수슬러지 처리에 있어서 적정 수분함량과 C/N비에 관한 연구)

  • 손현석;양원호;정문식
    • Journal of Environmental Health Sciences
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    • v.23 no.2
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    • pp.44-56
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    • 1997
  • When sewage sludge is treated by cornposting, higher moisture content and lower C/N ratio on sewage sludge is problems. This paper project to alesolve two problems. The almost trends in run 3 of MC 70% are similar to these in run 1 and 4 of MC 65%. A retention time of the highest temperature (>50$\circ$C) and increase rate of temperature in run 3 are an affinity to these in run 4. Particularly, decrease rate of temperature in run 3 is slower than others and this data shows the more activity of thermal microbial in run 3 than that in others. C/N ratio trends in run 3 shows slow reaction in initial stage but, after 9 days, is similar to that in run 1 and 4. Carbon trends in each run are a similarity to C/N ratio trends. Temperature, MC, carbon and C/N ratio trends in run 5, whose C/N ratio is 15, show less microbial activity than that in run 6, whose C/N ratio is 20. But temperature increase of the beginning stage and pH of the final stage in run 5 are greater than that in run 6. Final MC and carbon content in run 5 and 6 have a similar values. That is, final MC in run 5 and 6 is 49.39% and 48.97% and final carbon content in each run is 25.15% and 22.20%. Expecially, a temperature increase and C/N ratio decrease rate of the beginning stage in run 5 are greater than these in run 6. This shows the shorter lag time in run 5 than lag time in run 6.

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Denitrification of Synthetic Wastewater in Submerged Biofilter (침지식 여과조를 이용한 합성 폐수의 탈질화)

  • 오승용;조재윤;김인배
    • Journal of Aquaculture
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    • v.10 no.3
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    • pp.335-346
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    • 1997
  • Denitrification is one of the important processes of removing nitrate from in recirculating aquaculture systems. And this process is affected by many factors such as external organic carbon sources, hydraulic retention time (HRT), COD/NO3--N (C:N) ratio, etc. However, not many studies were done for the optimum conditions of denitrification in the recirculation system for aquaculture. Therefore, this study was conducted to find out the optimum removal condition of NO3--N using submerged denitrification biofilter. The combinations of two external organic carbon sources (glucose and methanol), two HRT (4 and 8-hour) and four differnent C : N ratios (3, 4, 5, 6) were tested. The removal efficiencies of NO3--N and total inorganic nitrogen (TIM) at 8-hour HRT were better than those at 4-hour's (P<0.05). The maximum removal efficiency of NO3--N by methanol (97.8%) was achieved at HRT and C : N ratio were 8-hour and 4.0 respectively. The efficiencies of methanol for the removal of NO3--N and TIN were always better than those of glucose (P<0.05). The maximum removal efficiencies of total inorgainc nitrogen (TIN) were gained at C : N ration of 5.0. The maximum removel efficiencies of TIN using methanol and glucose were 96.9% and 71.5% respectively. Anaerobic condition which is necessary for denitrification process was not made until the 8-hour HRT and higher C : N ratio (5.0). Removal of NO3--N at 4-hour HRT and C : N ration lower than 5.0 were inhibited by oxygen and/or low quantity of external organic carbon. Removal efficiencies of NO3--N were also inhibited by high C : N (6.0) ratio when HRT was 8-hour.

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The long-term decay rate and nutrient dynamics during leaf litter decomposition of Pinus densiflora and Pinus thunbergii (한반도 중부지역 조림지 소나무와 곰솔의 장기적 낙엽 분해율 및 분해과정에 따른 영양염류 동태변화)

  • Lee, Il-hwan;Jo, Soo-un;Lee, Young-sang;Won, Ho-yeon
    • Korean Journal of Environmental Biology
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    • v.39 no.3
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    • pp.374-382
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    • 2021
  • In the present study, we analyzed the decay rate and nutrient dynamics during leaf litter decomposition of Pinus densiflora and Pinus thunbergii in Gongju for 60 months, from 2014 to 2019. P. thunbergii leaf litter decomposed faster than that of P. densiflora. The decay constant of P. densiflora and P. thunbergii leaf litter after 60 months was 3.02 and 3.59, respectively. The initial C/N ratio of P. densiflora and P. thunbergii leaf litter were 14.4 and 14.5, respectively. After 60 months, C/N ratio of decomposing P. densiflora and P. thunbergii leaf litter decreased to 2.26 and 3.0, respectively. The initial C/P ratio of P. densiflora and P. thunbergii leaf litter were 144.1 and 111.3. After 60 months elapsed, the C/P ratio of decomposing P. densiflora and P. thunbergii leaf litter decreased to 40.1 and 45.8, respectively. After 60 months, the percentage of the remaining N, P, K, Ca, and Mg in decomposing P. densiflora leaf litter was 231.08, 130.13, 35.68, 48.58, and 36.03%, respectively. After 60 months, the percentage of the remaining N, P, K, Ca, and Mg in decomposing P. thunbergii leaf litter was 143.91, 74.02, 28.59, 45.08, and 44.99%, respectively. The findings of the present study provide an insight into the forest ecosystem function of coniferous forests through the analysis of the amount of nutrient transfer into the soil through a long-term decomposition process; this information is intended to be used as basic data for preparing counter measures for future climate and ecosystem changes.