• Journal of Sericultural and Entomological Science
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• v.49 no.2
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• pp.37-42
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• 2007

Lactoferrin, an ion-binding 80-kDa glycoprotein, has been suggested to have many biologic activities, such as facilitating ion absorption and having antimicrobial and anti-inflammatory effects. Several of these activities are likely to only be facilitated by human lactoferrin because they depend on the binding of human lactoferrin to specific receptor. To produce recombinant human lactoferrin to animal foods using transgenic silkworm, Bombyx mori L, we have cloned and sequenced the cDNA encoding for a human lactoferrin (HLf) from the mRNA in mammary tumor line (GI-101). As a result, the 2.5-kb fragment of HLf gene was cloned with pGEM-T vector and then this fragment was sequenced. In the nucleotide sequence analysis, single open reading frame of the 2,136-bp encoding for a polypeptide of 712 amino acid residues was detected. On the other hand, we constructed a recombinant plasmid(pPT-HLf), containing human lactoferrin gene for germ line transformation of the silkworm using a piggyBac transposon-derived vector. A nonautonomous helper plasmid encodes the piggyBac transposase. Approximately 6.7% of individuals in the G0 silkworms expressed green fluorescent protein (GFP). PCR analyses of GFP-positive silkworms (G0 and G1) revealed that independent insertions occurred frequently. Furthermore, Western blot analysis showed that the recombinant HLf expressed in hemolymph has the same molecular weight (80 kDa) as a native protein. On the basis of these experiments, expression of HLf in next generation of transgenic silkworm is now in process.

• Journal of Sericultural and Entomological Science
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• v.50 no.2
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• pp.87-92
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• 2012

We constructed the fibroin H-chain expression system to produce Discosoma sp. red fluorescent protein variant2 (DsRed2) in transgenic silkworm cocoon. Fluorescent cocoon could be made by fusing DsRed2 cDNA to the heavy chain gene and injecting it into a silkworm. The DsRed2 fusion protein, each with N- and C-terminal sequences of the fibroin H-chain, was designed to be secreted into the lumen of the posterior silk glands. The expression of the DsRed2/H-chain fusion gene was regulated by the fibroin H-chain promoter. The use of the 3xP3-driven EGFP cDNA as a marker allowed us to rapidly distinguish transgenic silkworms. The EGFP fluorescence became visible in the ocelli and in the central and peripheral nervous system on the seventh day of embryonic development. A mixture of the donor and helper vector was micro-injected into 1,020 Kumokjam, bivoltin silkworm eggs. We obtained 6 broods. The cocoon was displayed strong red fluorescence, proving that the fusion protein was present in the cocoon. Accordingly, we suggest that the DsRed2 fluorescence silk will enable the production of novel biomaterial based on the transgenic silk.

• Restorative Dentistry and Endodontics
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• v.33 no.5
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• pp.472-480
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• 2008

This study was conducted to evaluate the influence of the C-factor on the bond strength of a 6th generation self-etching system by measuring the microtensile bond strength of four types of restorations classified by different C-factors with an identical depth of dentin. Eighty human molars were divided into four experimental groups, each of which had a C-factor of 0.25, 2, 3 or 4. Each group was then further divided into four subgroups based on the adhesive and composite resin used. The adhesives used for this study were AQ Bond Plus (Sun Medical, Japan) and XenoIII (DENTSPLY, Germany). And composite resins used were fantasists (Sun Medical, Japan) and Ceram-X mono (DENTSPLY, Germany). The results were then analyzed using one-way ANOVA, a Tukey's test, and a Pearson's correlation test and were as follows. 1. There was no significant difference among C-factor groups with the exception of groups of Xeno III and Ceram-X mono (p<0.05). 2. There was no significant difference between any of the adhesives and composite resins in groups with C-factor 0.25, 2 and 4. 3. There was no correlation between the change in C-factor and microtensile bond strength in the Fantasista groups. It was concluded that the C-factor of cavities does not have a significant effect on the microtensile bond strength of the restorations when cavities of the same depth of dentin are restored using composite resin in conjunction with the 6th generation self-etching system.

• Communications for Statistical Applications and Methods
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• v.17 no.3
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• pp.459-471
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• 2010

Higher quality level is generally perceived by customers as improved performance by assigning a correspondingly higher satisfaction score. Usually, the quality level is measured by process capability indices. The index is used to determine whether a production process is capable of producing items within a specified tolerance. The third generation index $C_{pmk}$ is more powerful than two useful indices $C_p$ and $C_{pk}$. which have been widely used in six sigma industries to assess process performance. Most evaluations on process capability indices focus on point estimates, which may result in unreliable assessments of process performance. In this paper, we consider better testing procedure on assessing process capability index $C_{pmk}$ for practitioners to use in determining whether a given process is capable. It is easy to use the proposed method for assessing process capability index $C_{pmk}$. Whether a process is clearly normal or nonnormal, our bootstrap testing procedure could be applied effectively without the complexity of calculation. A numerical result based on our proposed method is illustrated.

• Journal of Life Science
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• v.28 no.10
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• pp.1212-1219
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• 2018

The critical role of heat shock protein 90 (Hsp90) in tumorigenesis led to the development of several first- and second-generation Hsp90 inhibitors, which have demonstrated promising responses in cancers. In this study, we found second-generation Hsp90 inhibitor BIIB021-resistant multidrug-resistant (MDR) human cancer cells, although BIIB021 was shown to be active in first-generation Hsp90 inhibitor 17-allylamino-17-demethoxygeldanamycin (17-AAG)-resistant MDR cells. MCF7-MDR and HeyA8- MDR cells were more resistant to BIIB021 than their parental counterparts, indicating that BIIB021 cannot be applicable to all cancer cells expressing MDR proteins. We revealed that dimethyl-celecoxib (DMC), one of the non-steroidal anti-inflammatory drugs (NSAIDs), potentiated cytotoxicity of BIIB021 against both BIIB021-resistant and BIIB021-sensitive MDR cells. The effectiveness of NSAIDs involving celecoxib and DMC in combination with BIIB021 led to the autophagic degradation/down-regulation of mutant p53 (mutp53) that overexpressed MDR cells and the suppression of Hsp70 induction. This resulted in sensitization of MDR cells to BIIB021. Moreover, autophagy induction by sulindac sulfide, another type of NSAID, and niclosamide, an FDA-approved anthelmintic drug, potentiated 17-AAG-mediated autophagic degradation/down-regulation of mutp53 and c-Myc, client proteins of Hsp90. Therefore, our results suggest that NSAIDs and niclosamide positively enhance the anticancer activity of Hsp90 inhibitors through an autophagic pathway. They may also be new candidates for sensitizing MDR cells to Hsp90 inhibitors.

• Korean journal of applied entomology
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• v.58 no.3
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• pp.239-249
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• 2019

This study was carried out to compare on the time of occurrence of 6 major rice insect pests [Lissorhoptrus oryzophilus Kusche, Oulema oryzae Kuwayama, Sogatella furcifera (Horvath), Nilaparvata lugens Stal., Cnaphalocrocis medinalis (Guenee), Mythimna separata Walker] in northern part of the Korean peninsula. Using growing degree days of the insects, we evaluated the number of occurrence generations and the time of occurrence. Over-wintering insects such as L. oryzophilus and O. oryzae showed different occurrence periods in northern regions. The occurrence period of the first generation adults was later in northeast regions than in Korean middle regions and more later both in northern alpines and in northern parts of east sea. In addition, the first adults of S. furcifera, N. lugens, C. medinalis, and M. separata occurred between June and early August. However, from late August to September, these insects showed the different occurrence periods in northern regions. Especially, the second adults of N. lugens were not occurred and the second to third generation adults of S. furcifera, C. medinalis, and M. separata showed similar occurrence properties. Based on these properties, the occurrence of major rice insect pests will be less in northeast regions, northern regions of east sea, northern inlands, and northern alpines of the Korean peninsula. However, comparing with their occurrences in northern regions of Gyenonggi and Gangwon provinces, the rice insect pests may show similar occurrence pattern in mid-korean mountains except for pyunggang and yangduk regions as well as in the southern and northern regions of Suyang-san.

• Proceedings of the IEEK Conference
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• 1998.10a
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• pp.1207-1210
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• 1998

차세대 DVD system의 audio 규격인 Dolby AC-3를 구현하는 방법으로 DSP 프로세서인 TMSC80을 사용하여 실시간 처리 가능한 하드웨어 바탕의 firmware 소프트웨어를 개발하는 방법으로 구현하고자 한다. 본 논문에서는 먼저 TMS320C80을 바탕으로 한 하드웨어 구현에 관해 논의한다. 하드웨어의 구조는 TMS320C80과 시스템 메모리로의 DRAM, 오디오 입력부인 ADC, 입력 데이터를 효과적으로 사용하기 위한 FIFO menory, 오디오 출력용인 dac, 디버깅 및 통신포트로 USB, RS-232,LPT와 MPEG-2 encoding보드 등 다른 보드와 연계를 위한 local-bus를 위한 dual port ram으로 구성된다. 오디오 입력은 최대 24bit 48kHz sampling까지 받을 수 있다.

• The Proceeding of the Korean Institute of Electromagnetic Engineering and Science
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• v.21 no.5
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• pp.21-32
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• 2010

60 Hz 자기장에서 예쁜꼬마선충(C. elegans)의 발생 및 생식에 미치는 영향을 조사하였다. 자기장 노출 조건은 $0{\sim}500\;{\mu}T$이며, 실험 온도는 선충의 일반적 배양 온도 범위($2 1.2{\pm}0.6^{\circ}C$)에서 일정하게 유지하였으며, 노출은 선충의 배양 기간 동안 지속되었다. C. elegas 야생형 N2 및 스트레스에 민감한 hsf-1와 crt-1 등의 돌연변이체들에서 실험에 사용한 노출 조건에 따른 생식력의 차이는 없었다. 여러 세대에 걸쳐 자기장에 노출한 경우에도 자기장에 의한 생식력의 변화는 없었다. 결론적으로, C. elegans는 크기가 매우 작으므로(길이 약 1 mm) 유도전류가 적게 발생하여, 자기장($-500\;{\mu}T$)에 의한 영향이 없는 것으로 사려된다.

• Proceedings of the Korean Information Science Society Conference
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• 2000.10a
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• pp.373-375
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• 2000

B2B, B2C, B2G, C2C로 구체화된 전자 상거래는 관련 국제 컨소시엄이 구축되고 그 유형에 있어서도 경매, 교환, 직구매, 입찰 등으로 다양해지는 등 이미 차세대 기업 비즈니스 모델로서의 위치를 확고히 하고 있고, 나아가 차별화 된 솔루션으로서 점원이 있는 시장, 실시간 인터넷 대금 납부 등의 서비스들이 국내외적으로 많이 모색되고 있는 실정이다. 본 논문에서는 비교쇼핑 에이전트를 지원하는 B2C 솔루션(ISPA:mIn Seon comparison Agent)을 제안하고 있는데, 비교 쇼핑 에이전트 역시 그러한 노력의 일례로서 이는 동종의 상품을 취급하는 여러 몰 간에도 그 제시 가격이 서로 다른 것에 착안하여 최저가격을 제시하는 사이트를 안내하는 기능을 한다. ISPA는 현 업계에서 웹 기반 시스템의 솔루션으로서 각광 받고 있는 EJB를 플랫폼으로 하며, 각 정보소스에 대한 정보 추출 규칙을 활용해서 온라인으로 비교 정보를 제공하는 종래의 인공지능의 접근과는 달리 J2EE 웹 개발 기술을 이용하여 오프라인으로 비교 정보를 구축하는 웹 기반 시스템으로써 에이전트를 지원한다. 나아가 핵심 클래스인 상품 클래스를 메타 데이터의 부분집합으로써 정의하는 기법을 적용하였는데 이는 개체 지향 패러다임의 시스템 구축 시에 전략적으로 응용될 수 있다.

• Information and Communications Magazine
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• v.30 no.10
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• pp.18-24
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• 2013

협력 지능형 교통 시스템 (C-ITS: Cooperative Intelligent Transportation System)은 차량이 도로 인프라 또는 다른 차량과 서로 통신하면서 전방의 교통사고 및 장애물과 주변 차량 정보를 공유하여 위험상황을 피할 수 있도록 사전에 경고하는 미래형 교통체계이다. C-ITS는 보행자 및 차량의 안전을 향상시키고 배출탄소량 감소 및 교통물류의 효율성을 증가시킬 수 있는 미래사회의 핵심 인프라가 될 전망이다. C-ITS의 성공적인 실현을 위해서는 다중 센서 융 복합 기반 교통정보 수집, 교통정보를 쌍방향으로 유통하기 위한 통합 무선 통신망, 스마트 기기와 이동통신망을 활용한 실시간 교통정보 수집 및 빅 데이터 처리와 주문형 서비스 제공 등의 핵심 기술 개발이 필요하다. 본 고에서는 협력형 교통 환경에서의 C-ITS 구조 및 관련 핵심 요소 기술을 소개하고, 앞으로 해결할 과제를 소개 한다.