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Anti-inflammatory Effects of Rumohra adiantiformis Extracts Fermented with Bovista plumbea Mycelium in LPS-stimulated RAW 264.7 Cells (LPS로 자극된 RAW 264.7 세포에서 찹쌀떡버섯 균사체로 생물전환된 루모라고사리 추출물의 항염증 효과)

  • Ji-Hye Hong;Eun-Seo Jang;Myung-Chul Gil;Gye Won Lee;Young Ho Cho
    • Journal of Life Science
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    • v.33 no.6
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    • pp.471-480
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    • 2023
  • This study was designed to evaluate the anti-inflammatory effects of Rumohra adiantiformis extracts fermented with Bovista plumbea mycelium (B-RAE) in LPS-stimulated RAW 264.7 cells. The total polyphenol and total flavonoid content of B-RAE were 379.26±7.77 mg/g and 50.85±3.08 mg/g, respectively. The results of measuring the antioxidant activity of B-RAE showed that it scavenges 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and superoxide anion radical in a dose-dependent manner. B-RAE inhibited nitric oxide (NO) production in a dose-dependent manner without affecting cell viability. The gene expression of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-lβ (IL-1β), and IL-6 was measured using real time quantitative reverse transcription PCR (qRT-PCR). We found that, compared to the LPS-treated group, B-RAE significantly reduced the mRNA levels of the pro-inflammatory cytokines in a concentration-dependent manner. The expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), the phosphorylation of transcription factors such as nuclear factor-κB (NF-κB), and the mitogen-activated protein kinase (MAPK) signaling pathway proteins were assessed using Western blot analysis. We found that B-RAE significantly suppressed the expression of iNOS and COX-2, but their expression was increased by LPS treatment. In addition, the phosphorylation of NF-κB and IκB, which was increased by LPS treatment, was reduced with B-RAE treatment. The effect of B-RAE on the phosphorylation of the MAPK signaling pathway proteins was measured, and the phosphorylation of extracellular signal-regulated kinase (ERK) and the p38 MAPK proteins decreased in a dose-dependent manner, while the phosphorylation of c-Jun N-terminal kinase (JNK) increased. These anti-inflammatory effects of B-RAE may thus have been achieved through the high antioxidant activity, the inhibition of NO production through the suppression of iNOS and COX-2 expression, the inhibition of the NF-κB pathway, and the suppression of pro-inflammatory cytokine expression.

A Study on the Effect of Metals on Bacteria Adhesion to Zeolite as Bio-media Materials (제올라이트를 이용한 생물막 형성시 미생물의 부착에 금속이 미치는 영향에 관한 연구)

  • Kim, Jae Keun;Park, In Sun;Park, Jae-Woo
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.29 no.3B
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    • pp.303-310
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    • 2009
  • Natural zeolite is widely used as sorbents and bio-media materials because it is cheap as well as it has efficient porous structures and large cation exchange. In this study, the effect of metal cations $(Na^+,\;Ca^{2+},\;Mg^{2+},\;Al^{3+})$ adsorbed to natural zeolite on the microorganism attachment was investigated. Metal-modified zeolites (MMZ) were prepared with 0.01 M, 0.02 M and 0.1 M NaCl, $CaCl_2$, $MgCl_2$ and $AlCl_3$ solutions respectively, which concentrations were equivalent to 10%, 20% and 100% of cation exchange capacity (CEC) of natural zeolite. Pseudomonas putida was used as microorganism which was cultivated in Beef Extract Medium at $26^{\circ}C$. The microorganism attachment to MMZ was increased more than natural zeolite. The amount of bacterial adhesion to MMZ and natural zeolite were $Mg^{2+}>natural>Na^+>Al^{3+}>Ca^{2+}$ under 10% of CEC, $Mg^{2+}>Ca^{2+}>Al^{3+}>natural>Na^+$ under 20% of CEC and $Ca^{2+}>Mg^{2+}>natural>Al^{3+}>Na^+$ under 100% of CEC. Especially, Mg-modified zeolite (Mg-MZ) showed the highest amount of bacterial adhesion, which increased the microorganism attachment 60% higher than natural zeolite under 10% of CEC. However, the amount of bacterial adhesion was decreased as the concentration of metal cations modified to zeolite were increased, showing that the increased amounts were 60% under 10% of CEC, 50% under 20% of CEC and 10% under 100% of CEC in Mg-MZ. Additionally, the effect of $Mg^{2+}$ in solution on the bacterial adhesion was investigated in order to compare it with the effect of $Mg^{2+}$ adsorbed to zeolite. The maximum quantity of bacterial adhesion to Mg-MZ was not different from the amount of microorganism attachment to the natural zeolite when $Mg^{2+}$ solution was added.

The factors to impact on the satisfaction of field practice among dental hygiene students (일부 치위생(학)과 학생들의 현장임상실습 만족도의 영향을 미치는 요인)

  • Sim, Seon-Ju;Baek, Ha-Oung;Um, Jung-Sun;Jung, Ha-Yan;Ji, Hey Mi;Hwang, Si-Nae;Han, Ji-Hey
    • Journal of Korean Academy of Dental Administration
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    • v.5 no.1
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    • pp.22-30
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    • 2017
  • Objective: The purpose is to study the factors to impact on the satisfaction of field practice among dental hygiene students and to provide the basic data for the quality improvement of the field practices for dental hygiene students. Methods: A self-reported questionnaires were filled out by 108 dental hygiene students in C city. Questionnaires were composed with the general characteristics, satisfaction to major, and satisfaction to field clinical practice. The data was analyzed by chi-square statistics using SPSS 20.0 program. Results: The higher satisfaction to communication with professor or seniors in department was associated with the higher satisfaction to field clinical practice (p<0.05). The higher satisfaction to field clinical practice hours per week was associated with the higher satisfaction to field clinical practice (p<0.05). The higher interest to major subjects and the satisfaction to the laboratory environment were associated with the higher satisfaction to field clinical practice (p<0.05). Conclusions: The higher satisfaction to major was associated with the higher satisfaction to field clinical practice. Therefore, the systematic curriculum development is needed to improve the satisfaction with major and clinical practice.

Study on the Chemical Composition of Lotus Root and Functional Evaluation of Fermented Lotus Root Drink (연근의 성분분석 및 연근 발효음료의 기능성 평가)

  • Bae, Man-Jong;Kim, Soo-Jung;Ye, Eun-Ju;Nam, Hak-Sik;Park, Eun-Mi
    • Journal of the Korean Society of Food Culture
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    • v.23 no.2
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    • pp.222-227
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    • 2008
  • This study examined the chemical composition of lotus root and functionally evaluated a fermented lotus root drink. Electron-donating ability using DPPH along with nitrite-scavenging ability were used to compare the antioxidative activities of unfermented and fermented lotus root drinks. The electron-donating abilities of the unfermented lotus root drink (1%) and fermented lotusroot drink (1%) were 22.55% and 23.88%, respectively. At pH 6.0, the nitrite-scavenging abilities of the unfermented lotus root drink and the fermented lotus root drink (100%) were 27.64% and 40.3%, respectively, and their scavenging ability increased in a dose-dependent manner at all pH values. In order to study the anti-obesity effects of the two drinks, male Sprague-Dawley rats were divided into four groups (A: basal diet, B: high fat diet, C: high fat diet+unfermented lotus root drink, D: high fat diet+fermented lotus root drink). Net weight gains were not significantly different among the four groups. Plasma total cholesterol concentrations significantly decreased in the groups receiving the unfermented and fermented lotus root drinks. Also, plasma total lipid and triglyceride contents were lower in the groups receiving the unfermented and fermented lotus root drinks as compared to the high fat diet group; however, the differences among the three groups were not significant.

The Effects of Treatment Dumbbell Exercise on Body Composition, Fitness, and Blood Lipid Profiles in Sarcopenic Elderly (미용덤벨 운동이 노인의 근감소증 예방을 위한 신체조성, 체력 및 혈중지질에 미치는 영향)

  • So, Wi-Young;Song, Mi-Soon;Cho, Be-Long;Park, Yeon-Hwan;Kim, Yeon-Soo;Lim, Jae-Young;Kim, Seon-Ho;Song, Wook
    • 한국노년학
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    • v.29 no.3
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    • pp.837-850
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    • 2009
  • Previous epidemiological studies reported that significant muscle loss is observed with advancing aging, called sarcopenia. This study is to investigate the effects of treatment dumbbell exercise on prevention of sarcopenia. The subjects were elderly between 60~70 years old who participated in J-Welfare senior center exercise program at J-gu in S-city and divided into control group(N=19) and exercise group(N=8). Treatment dumbbell exercise was performed 2 times per week for 12 weeks and body composition, fitness, and blood lipid profiles were measured before and after this program. There was no significance in body fat before and after 12 weeks of treatment dumbbell exercise, but there was significance in weight(F=4.312, p=0.048), BMI(F=4.532, p=0.043), and FFM(F=4.743, p=0.039). There was no significance in fitness such as arm curl(F=1.103, p=0.304), and back scratch(F=0.214, p=0.648), but there was significance in 2-minute step(F=33.638, p<0.001), chair stand(F=14.575, p=0.001), chair sit and reach(F=7.198, p=0.013), and 8-ft up and go(F=14.890, p=0.001). The variables of blood lipid profiles such as TC(F=0.030, p=0.864), TG(F=0.142, p=0.710), HDL(F=2.066, p=0.163), glucose (F=0.125, p=0.727), and HbA1c(F=0.945, p=0.340) has no significance. It was found that treatment dumbbell exercise has positive effects on body composition and fitness but has no positive effects on blood lipid profiles of the elderly.

Relationship Between Depression and Quality of Life in Elderly Women Living Alone: The Moderating and Mediating Effects of Social Support and Social Activity (여성독거노인의 우울과 삶의 질과의 관계: 사회적 지지, 사회적 활동의 조절효과 및 매개효과)

  • Lin, Qin Lan;Kim, Hee Kyung;Ann, Jung Sun
    • 한국노년학
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    • v.31 no.1
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    • pp.33-47
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    • 2011
  • The purpose of this study was to examine the moderating and mediating effects of social support and social activity on the relationship between depression and quality of life in elderly women living alone. Subjects were 129 elderly living alone at K city in C province, from June to July, 2010. The data was analyzed using the SPSS program for descriptive statistics, Pearson's correlation coefficient and stepwise multiple regression. The degree of depression of elderly living alone was above the average(2.65), and that of quality of life was average(2.80). The correlated factors of quality of life among elderly women living alone included depression(r=-.745, p=.004), social support(r=.544, p=.000), leisure activity(r=.480, p=.024), and economic activity(r=.711, p=.001). Social support was an important mediator between the depression and quality of life in elderly women living alone. The moderating effects of social support and social activity between depression and quality of life in elderly women living alone were not significant. This study suggests that social support considered in enhancing the quality of life programs designed for elderly living alone. Further research needs to be done to refine moderating and mediating effects of social support, social activity including leisure activity, economic activity and volunteer activity.

Le Moi naturel et la cosmogonie chez Paul Valéry : au point de vue de la mythologie indienne (폴 발레리Paul Valéry의 본성적 '자아'와 우주 발생론 : 인도 신화를 중심으로)

  • JEANG, Kwangheam
    • Korean Association for Visual Culture
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    • v.23
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    • pp.463-524
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    • 2013
  • En exprimant 'la découverte de l'homme', Valéry, dans la Philosophie de la danse, représente «un plaisir qui allait jusqu'à une sorte d'ivresse, et si intense parfois, qu'un épuisement total de ses forces, une sorte d'extase d'épuisement pouvait seule interrompre son délire, sa dépense motrice exaspérée». Dans le même sens du plaisir, Jayadéva, dans son dithyrambe du Gîta-Govinda, représente la danse de Harî, une des nombreuses formes de Vichnou. Excités par le brûlant désir des jeux de la volupté, Hari et son amante Râdhâ cherchent au cours de la danse Râsa l'énergie vitale. Voilà la source du plaisir mystérieux valéryen. Ensuite l'eau, «élément essentiel de toute vie», est la mesure du temps de même que le soleil, l'eau est le principe de l'harmonie comme celui du monde. Finalement, chez Valéry, sous les diverses infleunces de l'eau mythique, la mer devient l'Océan de lait, soit le lieu de naissance, soit la substance maternelle, soit l'essence da la création universelle. Or tout au long de 「La Dormeuse」, Valéry évoque l'image de 'Vichnou-Narayana' sous l'influence de la mythologie indienne. Et sous une autre influence de Flaubert, Valéry évoque « d'étranges mondes abstraits». Malgré tout, Valéry crée lui-même, dans 「La Dormeuse」, une nouvelle image d'un monde abstrait : 'Vichnou-Narayana' couché sur un lit de lotus, porté par les replis du grand serpent Ananta, qui élève au-dessus du dieu endormi méditant, ses sept têtes formant une éspèce de dais - du sein de Narayana, richement décoré d'un collier d'étoiles et d'une couronne de pierres précieuses en forme de disque, croit un lotus qui porte Brahma dans son calice ; Lakchmi est aux pieds de son divin époux. L'épisode des dieux indiens est à un stade encore plus avancé de la destruction du symbole. Ils sont réduits à des formes symboliques obscures, non commentées et même difficilement identifiables. Le dieu rose qui mord son orteil dans une attitude à la fois mystérieuse et grotesque, c'est Vichnou qui a, selon le vichnouisme, le premier rôle dans la création du monde. Il flottait avant la création sur les eaux, couché sur une feuille de figuier, sous la forme d'un jeune enfant qui porte son pied vers sa bouche. Cette scène évoque la méditation et le repli sur soi de la divinité avant le commencement. Valéry désigne la cosmogonie particulière d'une religion bien déterminée(le vichnouisme) sans la nommer et en la vidant de son sens pouratnt capital, laissant subsister un symbole guetté par le grotesque, un dieu en enfance ; d'autre part, cette cosmogonie est télescopée et intégrée par une cosmogonie d'origine différente : le désemboîtement des trois dieux renvoie à la théorie sivaiste du Lingam, l'arbre de vie. Les dieux de la tirinité iendienne se détachent les un après les autres et il ne reste plus que la fleur sous la garde de Vichnou. Le désemboîtement des dieux paraît bien se référer à cette conception, malgré l'absence du lingam. Enfin toute la forme veille ; et tous les yeux sont ouverts.

Strategic Issues in Managing Complexity in NPD Projects (신제품개발 과정의 복잡성에 대한 주요 연구과제)

  • Kim, Jongbae
    • Asia Marketing Journal
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    • v.7 no.3
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    • pp.53-76
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    • 2005
  • With rapid technological and market change, new product development (NPD) complexity is a significant issue that organizations continually face in their development projects. There are numerous factors, which cause development projects to become increasingly costly & complex. A product is more likely to be successfully developed and marketed when the complexity inherent in NPD projects is clearly understood and carefully managed. Based upon the previous studies, this study examines the nature and importance of complexity in developing new products and then identifies several issues in managing complexity. Issues considered include: definition of complexity : consequences of complexity; and methods for managing complexity in NPD projects. To achieve high performance in managing complexity in development projects, these issues need to be addressed, for example: A. Complexity inherent in NPD projects is multi-faceted and multidimensional. What factors need to be considered in defining and/or measuring complexity in a development project? For example, is it sufficient if complexity is defined only from a technological perspective, or is it more desirable to consider the entire array of complexity sources which NPD teams with different functions (e.g., marketing, R&D, manufacturing, etc.) face in the development process? Moreover, is it sufficient if complexity is measured only once during a development project, or is it more effective and useful to trace complexity changes over the entire development life cycle? B. Complexity inherent in a project can have negative as well as positive influences on NPD performance. Thus, which complexity impacts are usually considered negative and which are positive? Project complexity also can affect the entire organization. Any complexity could be better assessed in broader and longer perspective. What are some ways in which the long-term impact of complexity on an organization can be assessed and managed? C. Based upon previous studies, several approaches for managing complexity are derived. What are the weaknesses & strengths of each approach? Is there a desirable hierarchy or order among these approaches when more than one approach is used? Are there differences in the outcomes according to industry and product types (incremental or radical)? Answers to these and other questions can help organizations effectively manage the complexity inherent in most development projects. Complexity is worthy of additional attention from researchers and practitioners alike. Large-scale empirical investigations, jointly conducted by researchers and practitioners, will help gain useful insights into understanding and managing complexity. Those organizations that can accurately identify, assess, and manage the complexity inherent in projects are likely to gain important competitive advantages.

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Evaluations of Chinese Brand Name by Different Translation Types: Focusing on The Moderating Role of Brand Concept (영문 브랜드네임의 중문 브랜드네임 전환 방식에 대한 중화권 소비자들의 브랜드 평가에 관한 연구 -브랜드컨셉의 조절효과를 중심으로-)

  • Lee, Jieun;Jeon, Jooeon;Hsiao, Chen Fei
    • Asia Marketing Journal
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    • v.12 no.4
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    • pp.1-25
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    • 2011
  • Brand names are often considered as a part of product and important extrinsic cues of product evaluation, when consumers make purchasing decisions. For a company, brand names are also important assets. Building a strong brand name in the Chinese commonwealth is a main challenge for many global companies. One of the first problem global company has to face is how to translate English brand name into Chinese brand name. It is very difficult decision because of cultural and linguistic differences. Western languages are based on an alphabet phonetic system, whereas Chinese are based on ideogram. Chinese speakers are more likely to recall stimuli presented as brand names in visual rather than spoken recall, whereas English speakers are more likely to recall the names in spoken rather than in visual recall. We interpret these findings in terms of the fact that mental representations of verbal information in Chinese are coded primarily in a visual manner, whereas verbal information in English is coded by primarily in a phonological manner. A key linguistic differences that would affect the decision to standardize or localize when transferring English brand name to Chinese brand name is the writing system. Prior Chinese brand naming research suggests that popular Chinese naming translations foreign companies adopt are phonetic, semantic, and phonosemantic translation. The phonetic translation refers to the speech sound that is produced, such as the pronunciation of the brand name. The semantic translation involves the actual meaning of and association made with the brand name. The phonosemantic translation preserves the sound of the brand name and brand meaning. Prior brand naming research has dealt with word-level analysis in examining English brand name that are desirable for improving memorability. We predict Chinese brand name suggestiveness with different translation methods lead to different levels of consumers' evaluations. This research investigates the structural linguistic characteristics of the Chinese language and its impact on the brand name evaluation. Otherwise purpose of this study is to examine the effect of brand concept on the evaluation of brand name. We also want to examine whether the evaluation is moderated by Chinese translation types. 178 Taiwanese participants were recruited for the research. The following findings are from the empirical analysis on the hypotheses established in this study. In the functional brand concept, participants in Chinese translation by semantic were likely to evaluate positively than Chinese translation by phonetic. On the contrary, in the symbolic brand concept condition, participants in Chinese translation by phonetic evaluated positively than by semantic. And then, we found Chinese translation by phonosemantic was most favorable evaluations regardless of brand concept. The implications of these findings are discussed for Chinese commonwealth marketers with respect to brand name strategies. The proposed model helps companies to effectively select brand name, making it highly applicable for academia and practitioner. name and brand meaning. Prior brand naming research has dealt with word-level analysis in examining English brand name that are desirable for improving memorability. We predict Chinese brand name suggestiveness with different translation methods lead to different levels of consumers' evaluations. This research investigates the structural linguistic characteristics of the Chinese language and its impact on the brand name evaluation. Otherwise purpose of this study is to examine the effect of brand concept on the evaluation of brand name. We also want to examine whether the evaluation is moderated by Chinese translation types. 178 Taiwanese participants were recruited for the research. The following findings are from the empirical analysis on the hypotheses established in this study. In the functional brand concept, participants in Chinese translation by semantic were likely to evaluate positively than Chinese translation by phonetic. On the contrary, in the symbolic brand concept condition, participants in Chinese translation by phonetic evaluated positively than by semantic. And then, we found Chinese translation by phonosemantic was most favorable evaluations regardless of brand concept. The implications of these findings are discussed for Chinese commonwealth marketers with respect to brand name strategies. The proposed model helps companies to effectively select brand name, making it highly applicable for academia and practitioner.

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Effect of Quartz Porphyry and Hydroball Ratio on Growth of Some Wood Plant by Water Flooding Culture in Pot (목본식물의 분 담수 재배에서 맥반석 및 하이드로 볼의 비율이 식물의 생장에 미치는 영향)

  • Song, C.Y.;Moon, J.Y.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.21 no.1
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    • pp.15-28
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    • 2019
  • This study aimed to select the media for water flooding culture of Pinus thunbergii Parl., Juniperus chinensis L. var. sargentii Henry, Osmanthus fragrans Lour., and Chaenomeles japonica Lindl. ex Spach planting into quartz Porphyry and Hydroball. The survival rate of Pinus thunbergii Parl. and Chaenomeles japonica Lindl. ex Spach in hydroball 80%+quartz porphyry 20% was 100%, also the ratio of Juniperus chinensis L. var. sargentii Henry and Osmanthus fragrans Lour. in hydroball 20%+quartz porphyry 80% was 100% and 80%. However survival rate of full water and decomposed granite was less than 80% in Pinus thunbergii Parl., Juniperus chinensis L. var. sargentii Henry, and Chaenomeles japonica Lindl. ex Spach and thee rate of Osmanthus fragrans Lour was less than 50%. The increasing rate of fresh weight for Pinus thunbergii Parl. and Chaenomeles japonica Lindl. ex Spach in hydroball 80%+quartz porphyry 20% was 58.6% and 15.8% which was higher than others, and the fresh weight was increased as increasing the content of hydrobol. However the fresh weight of Juniperus chinensis L. var. sargentii Henry and Osmanthus fragrans Lour. in hydroball 20%+quartz porphyry 80% was 71.4% and 59.7% which was higher than others, and the fresh weight was increased as increasing the content of quartz porphyry. The increasing rate of fresh weight of decomposed granite as control was the lowest by 32.7%, 48.0%, 33.3% and 7.0%, respectively in Pinus thunbergii Parl., Juniperus chinensis L. var. sargentii Henry, Osmanthus fragrans Lour., and Chaenomeles japonica Lindl. ex Spach. Therefore the survival rate and fresh weight was lower at water and decomposed granite. However the survival rate and increasing fresh weight of Pinus thunbergii Parl. and Chaenomeles japonica Lindl. ex Spach was higher as increasing the rate of hydrobol. And the survival rate and fresh weight of Juniperus chinensis L. var. sargentii Henry and Osmanthus fragrans Lour was higher as increasing the rate of quartz porphyry.