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Analysis of Bacterial Community Structure in the Soil and Root System by 168 rRNA Genes (16S rDNA를 이용한 토양, 작물근계의 세균군집 구조해석)

  • Kim, Jong-Shik;Kwon, Soon-Wo;Ryu, Jin-Chang;Yahng, Chang-Sool
    • Korean Journal of Soil Science and Fertilizer
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    • v.33 no.4
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    • pp.266-274
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    • 2000
  • Understanding of microbial community structure in soil-root system is necessary to use beneficial soil and rhizosphere microbes for improvement of crop production and biocontrol. The knowledge of behavior and function of microbes in soil-root system plays a key role for the application of beneficial inocula. Because the majority of the intact bacteria in soil are unable to grow on nutrient media, both culturable and nonculturable bacteria have to be studied together. In our study, culture-independent survey of bacterial community in the soil-root system of red pepper fields was conducted by the sequence analysis of three universal clone libraries of genes which code for small-subunit rRNA (rDNA). Universal small subunit rRNA primers were used to amplify DNA extracted from each sample and PCR products were cloned into pGEM-T. Out of 27 clones sequenced, 25 clones were from domain bacteria. Two of the rDNA sequences were derived from eukaryotic organelles. Within the domain bacteria, several kingdoms were represented : the Proteobacteria (16 clones). Cytophyga-Flexibacter-Bacteroides group (2 clones). the high G+C content gram-positive group(1 clone) and 4 unknown clones.

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The Biological and Biochemical Characteristics of Staphylococcus epidermidis Isolated from Diseased Cultured Flounder, Paralichthys olivaceus (양식 넙치에서 분리한 Staphylococcus epidermidis의 생물학적 및 생화학적 특성)

  • Sim, Doo-Saing;Jung, Sung-Hee;Park, Hyung-Sook;Chun, She-Kyu
    • Journal of fish pathology
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    • v.7 no.1
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    • pp.23-36
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    • 1994
  • A total of 8 strains of Staphylococcus epidermidis isolated in land-marine tank system of Kyongnam and Kyongbuk. Prefecture were tested for the biological and biochemical characteristics. These strains were isolated from pathologic specimens of cultured flounder. Paralichthys olivaceus. Growth of the isolates was good on BHIA, HIA. Staphylococcus No. 110 and ETGP. Growth was good at NaCl concentration between 2.0 and 3.0%, about $30^{\circ}C$ and at pH values about 7.0. DNase and coagulase production were negative, and all isolates except FSJ-2 strain were positive in hemolysis. Urease was positive reaction, and novobiocin resistance was negative. Acid was produced anaerobically from glucose and maltose. All isolates except FSJ-19 strain produced weakly were negative in anaerobic acid production from mannitol. Acid was produced aerobically from glucose, fructose, galactose, sucrose, maltose and dextrine. But all isolates were not gas production. In characterization of clinically isolates, four different biotype codes were obtained when the all isolated were tested simultaneously. Four different antibiotic susceptibility patterns were obtained. On the basis of these characteristics, the isolates were identified as Staphylococcus epidermidis.

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Numerical Analysis on Wave Characteristics around Submerged Breakwater in Wave and Current Coexisting Field by OLAFOAM (파-흐름 공존장내 잠제 주변에서 OLAFOAM에 의한 파랑특성의 수치해석)

  • Lee, Kwang-Ho;Bae, Ju-Hyun;An, Sung-Wook;Kim, Do-Sam;Bae, Kee Seung
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.28 no.6
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    • pp.332-349
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    • 2016
  • OLAFOAM is the powerful CFD code and is an expanded version of $OpenFOAM^{(R)}$, for wave mechanics simulation. The $OpenFOAM^{(R)}$ does provide many solvers to correspond to each object of the numerical calculation in a variety of fields. OLAFOAM's governing equation bases on VARANS (Volume-Averaged Reynolds-Averaged Navier-Stokes) equation, and the finite volume method is applied to numerical techniques. The program is coded in C++ and run on the Linux operating system. First of all, in this study, OLAFOAM was validated for 1) wave transformation inside porous structure under bore and regular wave conditions, 2) wave transformation by submerged breakwater under regular wave condition, and 3) regular wave transformation and resultant vertical velocity distribution under current by comparison with existing laboratory measurements. Hereafter, this study, which is almost no examination carried out until now, analyzed closely variation characteristics of water surface level, wave height, frequency spectrum, breaking waves, averaged velocity and turbulent kinetic energy around porous submerged breakwater in the wave and current coexisting field for the case of permeable or impermeable rear beach. It was revealed that the wave height fluctuation according to current direction(following or opposing) was closely related to the turbulent kinetic energy, and others.

The Design Method of GNSS Signal Using the Analysis Result of Receiver Performance (수신 성능 분석을 이용한 위성항법 신호 설계 방안)

  • Jin, Mi-Hyun;Choi, Heon-Ho;Kim, Kap-Jin;Park, Chan-Sik;Ahn, Jae-Min;Lee, Sang-Jeong
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.37 no.6C
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    • pp.502-511
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    • 2012
  • As the importance of GNSS system increases, the necessity of independent system is increased also. When the independent GNSS system is required, GNSS signal design is necessary with requirement definition. This paper suggests the design method of GNSS signal using the analysis result of receiver performance. First, the candidates are defined based on the design elements. Then the receiver performance of the candidates is analyzed based on the performance evaluation parameters. The weights of performance evaluation parameter are defined in order to consider the receiver performance in a various aspects. Finally, the calculation of normalized performance evaluation parameters and weights are derived to obtain the compared value for signal selection. Spreading code, modulation method and carrier frequency are considered as design parameters. Also, correlation width, DLL PLL thermal noise jitter, frequency bandwidth and side lobe peak ratio are considered as performance evaluation parameters. And positioning performance, robustness to noise, bandwidth efficiency are considered as the performance aspects. This paper analyzes the performance of each candidate using software based simulator and suggest the method to compare objectively the performance of each candidates.

An Automatic Identification System of Biological Resources based on 2D Barcode and UCC/EAN-128 (2차원 바코드와 UCC/EAN-128을 이용한 생물자원 자동인식시스템)

  • Chu, Min-Seok;Ryu, Keun-Ho;Kim, Jun-Woo;Kim, Hung-Tae;Han, Bok-Ghee
    • The KIPS Transactions:PartD
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    • v.15D no.6
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    • pp.861-872
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    • 2008
  • As rapid development of computing environment, field of automatic identification research which interoperates with various physical objects and digital information is making active progress. Although the automatic identification system is widely used in various industries, application of automatic identification system in the field of medical health doesn't reach other industry. Therefore research in medical health supplies such as medical equipment, blood, human tissues and etc is on progress. This paper suggests the application of automatic identification technology for biological resources which is core research material in human genome research. First of all, user environment requirements for the introduction of automatic identification technology are defined and through the experiments and research, barcode is selected as a suitable tag interface. Data Matrix which is 2D barcode symbology is chosen and data schema is designed based on UCC/EAN-128 for international defecto standard. To showapplicability of proposed method when applied to actual environment, we developed, tested and evaluated application as following methods. Experiments of barcode read time at 196 and 75 below zero which is actual temperature where biological resources are preserved resulted read speed of average of 1.6 second and the data schema satisfies requirements for the biological resources application. Therefore suggested method can provide data reliability as well as rapid input of data in biological resources information processing.

A Road Luminance Measurement Application based on Android (안드로이드 기반의 도로 밝기 측정 어플리케이션 구현)

  • Choi, Young-Hwan;Kim, Hongrae;Hong, Min
    • Journal of Internet Computing and Services
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    • v.16 no.2
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    • pp.49-55
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    • 2015
  • According to the statistics of traffic accidents over recent 5 years, traffic accidents during the night times happened more than the day times. There are various causes to occur traffic accidents and the one of the major causes is inappropriate or missing street lights that make driver's sight confused and causes the traffic accidents. In this paper, with smartphones, we designed and implemented a lane luminance measurement application which stores the information of driver's location, driving, and lane luminance into database in real time to figure out the inappropriate street light facilities and the area that does not have any street lights. This application is implemented under Native C/C++ environment using android NDK and it improves the operation speed than code written in Java or other languages. To measure the luminance of road, the input image with RGB color space is converted to image with YCbCr color space and Y value returns the luminance of road. The application detects the road lane and calculates the road lane luminance into the database sever. Also this application receives the road video image using smart phone's camera and improves the computational cost by allocating the ROI(Region of interest) of input images. The ROI of image is converted to Grayscale image and then applied the canny edge detector to extract the outline of lanes. After that, we applied hough line transform method to achieve the candidated lane group. The both sides of lane is selected by lane detection algorithm that utilizes the gradient of candidated lanes. When the both lanes of road are detected, we set up a triangle area with a height 20 pixels down from intersection of lanes and the luminance of road is estimated from this triangle area. Y value is calculated from the extracted each R, G, B value of pixels in the triangle. The average Y value of pixels is ranged between from 0 to 100 value to inform a luminance of road and each pixel values are represented with color between black and green. We store car location using smartphone's GPS sensor into the database server after analyzing the road lane video image with luminance of road about 60 meters ahead by wireless communication every 10 minutes. We expect that those collected road luminance information can warn drivers about safe driving or effectively improve the renovation plans of road luminance management.

A Study on the Medium Designator In Non-book Materials (비도서자료의 매체표시에 관한 연구)

  • Nam Tae Woo
    • Journal of the Korean Society for Library and Information Science
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    • v.15
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    • pp.119-140
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    • 1988
  • This paper is the study on the Medium Designator in Non-book materials. Main contents of this study were as follows; 1. The medium designator serves to indicate the class of material to which an item belongs. This is used to give an 'early warning' ;to the catalogue user. 2. This medium designator may be further divided into two elements ; a general material designation (GMD), for example video-recording, and a specific material designation (SMD), for example, videodisc. 3. GMD: In cataloging, a term indicating the broad class of material to which a bibliographic item belongs, such as 'motion picture', and SMD : In descriptive cataloging, a term indicating the special class of material (usually the class of physical object) to which a biblographic item belongs, such as videocassette. 4. Locating the medium designator after the title proper was not prescribed until ISBD(G) and AACR2. In pre-ISBD(G) codes, the ,early, warning type of medium designator was placed after all title information. But in AACR2, the medium designator is placed after the title proper, but before parellel title and other title information. 5. In Terminology, Two separate lists of designations are given in AACR2, l.1C1, one for British and one for North American use. The British list contains fewer terms, and uses generic categories to group together some of the North American list. 6. The problem of where to place the medium designator might be circumvented by using some kind of early alerting device other than a formal element of biblliographic description. Various alternatives have been suggested. A more popular device is the provision of symbols or 'media code' which are part of the call number and indicate the porticular medium type. 'Colour-coding' the use of used by some libraries but is now longly discouraged. 7. According to Frost. The medium designatorhas been generally reeognized as serving three functions; 1) as a statement of the nature or basic format of the item cataloged and thus as a meant of informing the user as to the type of material at hand; 2) as a description of the physical charaetistics of medium and as a means of alerting the user to equipment needed to make use of the item. 3) as a device to distinguish different physical formats which share the same title. 8. AACR2 raises some problems which decision makers have neet had to face preriously It provides a GMD for every item in the collection including books and it makes the application of any or all GMD's optional.

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Evaluation of Radiation Dose for Dual Energy CBCT Using Multi-Grid Device (에너지 변조 필터를 이용한 이중 에너지 콘빔 CT의 선량 평가)

  • Ju, Eun Bin;Ahn, So Hyun;Cho, Sam Ju;Keum, Ki Chang;Lee, Rena
    • Progress in Medical Physics
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    • v.27 no.1
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    • pp.31-36
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    • 2016
  • The paper discusses radiation dose of dual energy CT on which copper modulation layer, is mounted in order to improve diagnostic performance of the dual energy CT. The radiation dose is estimated using MCNPX and its results are compared with that of the conventional dual energy CT system. CT X-ray spectra of 80 and 120 kVp, which are usually used for thorax, abdominal, head, and neck CT scans, were generated by the SPEC78 code and were used for the source specification 'SDEF' card for MCNPX dose modeling. The copper modulation layer was located 20 cm away from a source covering half of the X-ray window. The radiation dose was measured as changing its thickness from 0.5 to 2.0 mm at intervals of 0.5 mm. Since the MCNPX tally provides only normalized values to a single particle, the dose conversion coefficients of F6 tally for the modulation layer-based dual energy CBCT should be calculated for matching the modeling results into the actual dose. The dose conversion coefficient is $7.2*10^4cGy/output$ that is obtained from dose calibration curve between F6 tally and experimental results in which GAFCHORMIC EBT3 films were exposed by an already known source. Consequently, the dose of the modulation layer-based dual energy cone beam CT is 33~40% less than that of the single energy CT system. On the basis of the results, it is considered that scattered dose produced by the copper modulation layer is very small. It shows that the modulation layer-based dual energy CBCT system can effectively reduce radiation dose, which is the major disadvantage of established dual energy CT.

The Design of Wireless Underwater Telephone -Analysis of Underwater Background Noise for Wireless Underwater Telephone Design - (수중 무선 전화기의 설계 - 수중무선전화기 설계를 위한 수중소음분석을 중심으로-)

  • 박문갑;윤갑동;김석제;윤종락
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.37 no.4
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    • pp.302-307
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    • 2001
  • The underwater background noise measured in Geoje and Tongyoung diving fishing ground from May to December, 2000 and analyzed to get optimum carrier frequency and transmitter power level for underwater wireless telephone design. The results obtained are summarized as follows: 1. At the Geoje and Tongyoung diving fishing ground, the lowest ambient noise band was 25~30kHz with 57dB and 52dB re 1$\mu$Pa, respectively. 2. At the Geoje and Tongyoung diving fishing ground, the lowest noise band during fishing activity was 67dB and 62dB re 1$\mu$Pa, respectively. 3. At the Geoje diving fishing ground, the noise of water jetter which is a digging machine for subbottom shells was 102dB re 1$\mu$Pa. 4. Considering the design parameters of underwater wireless telephone, it is found that the optimum carrier frequency band is around 30kHz and the transmitter source level should be at least 131dB re 1$\mu$Pa for 500m range telephone.

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REPLACEMENT OF A PHOTOMULTIPLIER TUBE IN A 2-INCH THALLIUM-DOPED SODIUM IODIDE GAMMA SPECTROMETER WITH SILICON PHOTOMULTIPLIERS AND A LIGHT GUIDE

  • KIM, CHANKYU;KIM, HYOUNGTAEK;KIM, JONGYUL;LEE, CHAEHUN;YOO, HYUNJUN;KANG, DONG UK;CHO, MINSIK;KIM, MYUNG SOO;LEE, DAEHEE;KIM, YEWON;LIM, KYUNG TAEK;YANG, SHIYOUNG;CHO, GYUSEONG
    • Nuclear Engineering and Technology
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    • v.47 no.4
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    • pp.479-487
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    • 2015
  • The thallium-doped sodium iodide [NaI(Tl)] scintillation detector is preferred as a gamma spectrometer in many fields because of its general advantages. A silicon photomultiplier (SiPM) has recently been developed and its application area has been expanded as an alternative to photomultiplier tubes (PMTs). It has merits such as a low operating voltage, compact size, cheap production cost, and magnetic resonance compatibility. In this study, an array of SiPMs is used to develop an NaI(Tl) gamma spectrometer. To maintain detection efficiency, a commercial NaI(Tl) $2^{\prime}{\times}2^{\prime}$ scintillator is used, and a light guide is used for the transport and collection of generated photons from the scintillator to the SiPMs without loss. The test light guides were fabricated with polymethyl methacrylate and reflective materials. The gamma spectrometer systems were set up and included light guides. Through a series of measurements, the characteristics of the light guides and the proposed gamma spectrometer were evaluated. Simulation of the light collection was accomplished using the DETECT 97 code (A. Levin, E. Hoskinson, and C. Moison, University of Michigan, USA) to analyze the measurement results. The system, which included SiPMs and the light guide, achieved 14.11% full width at half maximum energy resolution at 662 keV.