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Correlations among Anthropometric Measurements, Serum Lipid Levels and Nutrient Intake in Female University Students

  • Cheong, Sun-Hee;Chang, Kyung-Ja
    • Journal of Community Nutrition
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    • v.4 no.3
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    • pp.151-158
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    • 2002
  • The purpose of this study was to investigate the correlations among the anthropometry, serum lipid levels and nutrient intake in Korean female university students. The subjects were 119 female students at a university located in Incheon. This study was conducted using a self-administered questionnaire. Anthropometric data were measured and blood lipid levels were analyzed. Nutrient intake collected from 3 day-recalls was analyzed by the Computer Aided Nutritional Analysis Program. The data were analyzed by SPSS 10.0 program. Average age, height and weight of the subjects were 20.9 years, 160.1cm and 54.3kg, respectively. Average serum TG (triglyceride), total cholesterol, HDL-C (high density lipoprotein-cholesterol) and LDL-C (low density lipoprotein-cholesterol) levels of the subjects were 69.47mg/dl, 146.85 mg/dl, 50.49mg/dl and 82.52mg/dl, respectively. Average AI (atherogenic index) of the subjects was 2.03, which was in the normal range based on risk values. Average intake of most nutrients except protein, vitamin B$_1$, vitamin C and phosphorus were lower than the Korean RDA. Especially calcium and iron intakes of the subjects were under 65% of the Korean RDA. Serum TG, total cholesterol and LDL-C levels were negatively correlated with DBP (diastolic blood pressure). HDL-C/LDL-C and HDL-C/total cholesterol were positively correlated with height. Age was positively correlated with phosphorus intake. DBP of the subjects was positively correlated with calcium and iron intakes. Serum TG level was positively correlated with total cholesterol, HDL-C, LDL-C and AI, while negatively correlated with HDL-C/total cholesterol. Total cholesterol level was positively correlated with HDL-C, LDL-C and AI, while negatively correlated with HDL-C/LDL-C, HDL-C/total cholesterol. HDL-C level was positively correlated with LDL-C, HDL-C/LDL-C and HDL-C/total cholesterol, while negatively correlated with AI. LDL-C level was negatively correlated with HDL-C/LDL-C and HDL-C/total cholesterol, while positively correlated with AI HDL-C/LDL-C ratio was positively correlated with HDL-C/total cholesterol and AI. HDL-C/total cholesterol was negatively correlated with AI. Fat intake was positively correlated with total cholesterol, HDL-C level, and vitamin B$_2$ intake was positively correlated with TG, HDL-C/LDL-C. Therefore, nutrition education is necessary for female university students to promote the lipid profile and to optimize the nutritional status. (J Community Nutrition 4(3) : 151∼158, 2002)

Effects of Roasting Temperature on the Antinutrients and Functional Properties of Sesame Protein Isolates (볶음온도가 참깨 분리단백질의 항영양인자와 기능성에 미치는 영향)

  • Kim, Jean;Park, Jyung-Reung;Jeon, Jeong-Ryae
    • Journal of the East Asian Society of Dietary Life
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    • v.9 no.4
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    • pp.435-441
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    • 1999
  • This study was designed to investigate the effects of roasting temperatures on the antinutritional factors and functional properties of sesame protein isolates. The protein contents a sesame protein isolates (SPI) prepared at roasting temperatures of 5$0^{\circ}C$, 10$0^{\circ}C$, 15$0^{\circ}C$ and 20$0^{\circ}C$ were 65.5%, 66.6%. 68.9% and 64.1%, respectively. Total phenolic compounds, condensed tannin and phytate contents of SPI was increased from 5$0^{\circ}C$ to 20$0^{\circ}C$. From color measurements, higher roasting temperature decreased 'L' and 'b' values significantly, but 'a' value was increased. The bulk density, fat absorption and water absorption of SPI was increased as the temperature of roasting was increased. Sesame protein isolates prepared by roasting at 5$0^{\circ}C$, 10$0^{\circ}C$ and 15$0^{\circ}C$ had higher emulsifying activity than those prepared by roasting at 20$0^{\circ}C$. Foaming capacity of SPI was not changed by roasting up to 10$0^{\circ}C$, but this property was reduced dramatically when roasted at 20$0^{\circ}C$.

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Effect of Temperature at Different Performance Stages of Aphelinus varipes (Hym. Aphelinidae) Parasitizing the Green Peach Aphid, Myzus persicae (복숭아혹진딧물에 기생하는 목화면충좀벌의 발육단계별 온도영향)

  • Choi, Yong Seok;Hwang, In Su;Park, Deog Gee;Choe, Kwang Ryul
    • Korean journal of applied entomology
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    • v.51 no.4
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    • pp.343-348
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    • 2012
  • Developmental period, mummification, pupal mortality, and sex ratio of Korean strain of Aphelinus varipes F$\ddot{o}$rster parasitizing the green peach aphid, Myzus persicae were studied at 20, 25, 30, and $35^{\circ}C$ in controlled climate cabinets. The plastic container with a leaf disc of sweet pepper (Capsicum annuum L.) was used as experimental units. Green peach aphids at different developmental stages were reared in four different temperatures and presented to A. varipes for 12 hours. A female adult of A. varipes was allowed to parasitize for 12 hours in each plastic petri dish. The periods from egg to adult of A. varipes were 18.3 days, 14.7 days, 10.4 days, and 9.3 days at $20^{\circ}C$, $25^{\circ}C$, $30^{\circ}C$, and $35^{\circ}C$, respectively. The pupal mortalities of A. varipes were 0%, 1.5%, 2.8%, and 10.2% at $20^{\circ}C$, $25^{\circ}C$, $30^{\circ}C$, and $35^{\circ}C$, respectively. The mummifications of A. varipes were significantly different in all developmental stages of the aphid. The mummification of A. varipes parasitizing green peach aphid was the highest at 1st and 2nd in $20^{\circ}C$ and $25^{\circ}C$, but the highest at 3rd (84.4-85.0%) in $30^{\circ}C$ and $35^{\circ}C$. The host feedings of A. varipes were $20^{\circ}C$ (12.5%), $25^{\circ}C$ (17.3%), $30^{\circ}C$ (16%), and $35^{\circ}C$ (10.9%), respectively. The sex ratios of A. varipes female were $20^{\circ}C$ (49%), $25^{\circ}C$ (46%), $30^{\circ}C$ (69%), and $35^{\circ}C$ (60%), respectively.

The Microstructure Control of SiC Ceramics Containing Porcelain Scherben (자기파를 함유한 SiCwlf 세라믹스의 미세구조 제어)

  • 이성희
    • Journal of the Korean Ceramic Society
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    • v.32 no.5
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    • pp.626-634
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    • 1995
  • The SiC-porcelain powder mixtures containing 51.9wt% SiC are produced as by-products from the surface abrasion process of porcelain cores. This raw powders were used as starting materials for the synthesis of SiC containing ceramics. The specimen, which was fired at 135$0^{\circ}C$ from raw powders, had SiC, $Al_{2}O_{3}$, , cristobalite, mullite as crystalline phases, and the fractured microstructure showed dispersed SiC crystalline particles almost wetted with glassy matrix and spherical pores. Although the oxidation of SiC containing powder compacts wetted with glassy matrix and spherical pores. Although the oxidation of SiC containing powder compacts started at the range of 600~80$0^{\circ}C$ form the analysis of weight gain, the presence of $SiO_{2}$ crystallien phase and cristobalite was confirmed at 100$0^{\circ}C$ by XRD analysis. Mullitization of specimens was accelerated by preheating before the final firing. The specimen sintered at 135$0^{\circ}C$ after 100$0^{\circ}C$ preheating consisted of SiC, cristobalite, mullite as crystalline phases, and revealed 2.24g/$cm^{3}$ bulk density, 11.73% water adsorption, porous microstructure with small amount of glassy phase. SiC contents of specimens, which was 51.9 wt% in the raw powders, reduced to 37~22 wt% after firing at 135$0^{\circ}C$ depending on the preheating condition.

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Characteristics of Sensible Heat Storage for Gravel (자갈의 현열축열 특성)

  • 이귀현
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 2002.02a
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    • pp.564-572
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    • 2002
  • 본 연구에서는 크기가 서로 다른 자갈로 이루어진 3종류의 축열 매체에 대한 현열축열 특성을 조사하였으며, 그 연구 결과를 요약하면 다음과 같다. (1) 제 1 종 자갈: 축열조로 공급되는 공기의 온도가 5$0^{\circ}C$와 62$^{\circ}C$ 일 때, 축열조 출구의 공기온도가 33$^{\circ}C$에 도달될 때까지 가열되는 축열시간은 각각 170분과 130분이었으며, 방열시간은 각각 210분 및 250분으로 나타났다. 또한, 방열시 출열조 출구의 최고 공기 온도는 공급공기의 온도가 5$0^{\circ}C$와 62$^{\circ}C$ 일 때 각각 34.5$^{\circ}C$ 및 36.5$^{\circ}C$였으며, 출구 공기온도 33$^{\circ}C$이상을 기준으로 한 열 회수 시간은 각각 100분 및 115분으로 나타났다. (2) 제 2종 자갈: 축열조에 공급되는 공기의 온도가 52$^{\circ}C$와 64$^{\circ}C$ 일 때, 축열조 출구의 공기온도가 33$^{\circ}C$에 도달될 때까지 가열되는데 소요된 시간은 각각 175분과 140분이었고, 방열에 소요된 시간은 각각 215분 및 250분으로 나타났다. 방열과정 동안 축열조 출구의 최고 공기온도는 공급공기의 온도가 52$^{\circ}C$$65^{\circ}C$ 일 때 각각 35$^{\circ}C$ 및 38$^{\circ}C$였다. 축열조 출구의 공기온도 33$^{\circ}C$를 기준으로 한 열 회수 시간은 유입공기 온도가 52$^{\circ}C$ 및 64$^{\circ}C$ 일 각각 120분 및 140분으로 나타났다. (3) 제 3종 자갈: 축열조로 공급되는 공기의 온도가 52$^{\circ}C$와 64$^{\circ}C$ 일 때, 축열조 출구의 공기온도가 33$^{\circ}C$에 도달될 때까지 가열되는데 소요된 시간은 가열공기의 온도가 52$^{\circ}C$와 64$^{\circ}C$ 일 때 각각 180분과 150분이었고, 방열에 소요된 시간은 각각 240분 및 270분으로 나타났다. 방열과정 동안 축열조 출구의 최고 공기온도는 가열 공급공기의 온도가 52$^{\circ}C$$65^{\circ}C$일 때 각각 35.5$^{\circ}C$ 및 39.5$^{\circ}C$였다. 출구 공기온도 33$^{\circ}C$이상을 기준으로 한 에너지 회수시간은 유입공기 온도가 52$^{\circ}C$ 및 64$^{\circ}C$일 때 각각 140분 및 160분으로 나타났다. 이와 같이 자갈이 작을수록 축열조 출구의 공기온도가 기준온도 33$^{\circ}C$에 도달되는 시간이 길었으며, 이것은 축열조내의 공극이 작고 비중량이 커 자갈층을 가열시키는 축열시간이 길어지기 때문인 것으로 사료된다. 또한 작은 자갈일수록 방열시간도 다소 길어져 회수 가능 열에너지가 큰 것으로 나타났다.

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Effect of Temperature on the Quality and Storability of Cherry Tomato during Commercial Handling Condition (유통중 온도관리가 방울토마토의 품질과 저장성에 미치는 영향)

  • Islam, Mohammad Zahirul;Kim, Young-Sik;Kang, Ho-Min
    • Journal of Bio-Environment Control
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    • v.21 no.2
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    • pp.88-94
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    • 2012
  • This study was carried out in order to investigate the effect of temperature of treatment and storage on the longevity of 'Unicorn' tomatoes of light red maturity stage during commercial handling conditions encountered while exporting over long distances. Tomato stored at $5^{\circ}C$ and $11^{\circ}C$ temperature with 85% relative humidity by pre-treating handling temperature that was using from field to before shipment as a winter temperature $5^{\circ}C$, spring temperature $11^{\circ}C$ and summer temperature $25^{\circ}C$ for 3 days. On the final storage day, $25^{\circ}C/11^{\circ}C$ (treated/stored) tomatoes showed the highest respiration and ethylene production rate; whereas the lowest respiration and ethylene production rate was found for $5^{\circ}C/5^{\circ}C$ treated and stored tomatoes. Tomatoes treated and stored at $5^{\circ}C/5^{\circ}C$ showed higher marketability, without evidence of fungal rot, decay or spots for 23 days. The fresh weight loss under all treatment conditions increased gradually during $5^{\circ}C$ and $11^{\circ}C$ storage temperatures. The higher firmness and soluble solids were determined from $5^{\circ}C/5^{\circ}C$ and $5^{\circ}C/11^{\circ}C$ treated and stored tomatoes repectively, than from others tomatoes on the final day of storage. In addition, $5^{\circ}C/5^{\circ}C$ tomatoes showed higher vitamin C contents than tomatoes stored at other temperatures, on the final day of storage. As the ripening and storage period progressed, the titratable acidity increased, but declined (P < 0.05) thereafter, due to over ripe tomatoes under all treatment conditions. These results show that $5^{\circ}C/5^{\circ}C$ treated and stored light red maturity stages of 'Unicorn' tomatoes are optimum to export because they show the highest storability and marketability. Moreover, the marketability of light red maturity stage of 'Unicorn' tomato maintained for 2 weeks in $25^{\circ}C/11^{\circ}C$ treated and stored temperature that might be the export temperature from Korea to Japan in summer season. This research result could be useful in helping tomato growers and exporters to get optimum market value by satisfying the buyer and consumer with a fresher product.

Studies on Lipids in Fresh-Water Fishes 6. Comparison of Lipid Components between Wild and Cultured Snakehead, Channa argus (담수어의 지질에 관한 연구 6. 천연 및 양식 가물치의 지질성분 비교)

  • CHOI Jin-Ho;BAE Tae-Jin;BYUN Dae-Seo;YOON Tai-Heon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.18 no.4
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    • pp.309-315
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    • 1985
  • This study was designed to compare the lipid components between wild and cultured snakehead, Channa argus. The lipid components of cultured snakehead were analyzed and compared with that of wild snakehead. In both edible portion and viscera, the lipid content in cultured, snakehead was higher than that in wild one. In the fatty acid composition of neutral lipid in edible portion, percentages of $C_{14:0},\;C_{16:0},\;C_{18:1},\;C_{20:5},\;C_{22:5}\;and\;C_{22:6}$ in cultured snakehead were higher than those in wild one, while percentages of $C_{18:0},\;C_{16:1},\;C_{18:2},\;C_{18:3},\;C_{20:4}\;and\;C_{22:4}$ lower. In the case of phospholipid in edible portion, percentages of $C_{16:0},\;C_{18:1}\;and\;C_{22:6}$ in cultured snakehead were higher than those in wild one, while percentages of $C_{16:1},\;C_{18:2},\;C_{18:3},\;C_{20:4},\;C_{22:4}\;and\;C_{22:5}$ lower. The unsaturation (TUFA/TSFA) and w3 highly unsaturated fatty acid content (w3 HUFA) of neutral lipid in cultured snakehead were higher than those in wild one, while those of phospholipid lower. The essential fatty acid contents (TEFA) of both neutral and phospholipids in wild snakehead were higher than those in cultured one. In the ratio (A/B) of fatty- acid content (A) in cultured snakehead to that (B) in diet, the A/B ratios of $C_{18:2\;w6},\;C_{18:3\;w3},\;C_{20:5\;w3}\;and\;C_{22:6\;w3}$ were 0.3 to 2.5 times, and it is considered to be related to the biosynthesis of polyenoic acids and growth rate of cultured snakehead.

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Effects of Packing Materials, tight Condition and Storage Temperature on MAP Storage of Chicon (MAP저장시 포장재 종류, 광의 유무, 및 저장온도가 치콘 저장성에 미치는 영향)

  • Bae Jong Hyang;Park Kuen Woo;Kang Ho-Min
    • Journal of Bio-Environment Control
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    • v.14 no.2
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    • pp.69-75
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    • 2005
  • The storability of chicon was compared by packing it with PE box, wrap, LDPE (low density polyethylene) film that was 25 and 50um thickness, respectively and storing at 1 and $10^{\circ}C$ under light and dark conditions. The visual quality depending on dehydration was deteriorated at more than $2\%$ weight loss during storage. In packing treatments, chicon packed with PE box lost fresh weight to $3\%\;at\;10^{\circ}C\;and\;2\%\;at\;1^{\circ}C$, while non- penetrated film treatment, wrap, 25 and 50um thickness LDPE film, showed less than $1\%$ weight loss. The carbon dioxide concentration in package was $3\~4\%\;in\;50{\mu}m$ LDPE film at $1^{\circ}C\; and\;25um$ LDPE film at $10^{\circ}C$. The ethylene concentration in 50um LDPE film at $1^{\circ}C\;and\;25{\mu}m$ LDPE film at $10^{\circ}C$ was approximately 0.3 ppm and 0.5 ppm, respectively. Chiton stored in dark condition didn't turn to green, but it fumed green only in 3 days at $10^{\circ}C$ and in 6 days at $1^{\circ}C$ under light condition. The greening of chicon was less, the packing materials was thicker. The chlorophyll content represented the degree of greening showed less at $1^{\circ}C$ then at $10^{\circ}C$. The coefficient of correlation(r) between chlorophyll content and carbon dioxide concentration in package was 0.926 at $1^{\circ}C$ and 0.997 at $10^{\circ}C$. The visual quality except greening of packed chicon was maintained at $1^{\circ}C$ better than $10^{\circ}C$, and it was shown highest grade packed with $50{\mu}m$ LDPE film at $1^{\circ}C$ and packed with 25um LDPE film at $10^{\circ}C$. The vitamin C content in packed chicon was kept higher at $1^{\circ}C$ on storage temperatures, and 25um and 50um LDPE film on packing materials. According to these results, it can be proper condition for storage and marketing of chicon that 50um LDPE film at $1^{\circ}C$ and 25um LDPE film at $10^{\circ}C$. And dark condition is necessary to store chicon because it should turn green under tiny light condition.

Resolution of the Triacylglycerols Containing Conjugate Trienoic Acids into Their Molecular Species by HPLC in the Reversed-phase and Silver Ion Mode (Reversed-phase 및 $Ag^{+}$-HPLC에 의한 Conjugate Trienoic Acid 함유(含有) Triacylglycerol 분자종(分子種)의 상호분리(相互分離))

  • Kim, Seong-Jin;Woo, Hyo-Kyeng;Joh, Yong-Goe
    • Journal of the Korean Applied Science and Technology
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    • v.18 no.3
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    • pp.197-213
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    • 2001
  • Conjugate trienoic acids (CTA) occurred in triacylglycerols (TGs) of the seed oils of Trichosanthes kirilowii, Momordica charantia and Aleurites fordii, and they were easily converted to their methyl esters in a mixture of sodium methoxide-methanol without any structural destruction. The main fatty acids in triacylglycerol (TG) fraction of the seed oils of Trichosanthes kirilowii are $C_{18:2{\omega}6}$ (32.2 mol %), $C_{18:3{\;}9c.11t,13c}$ (38.0 mol %) and $C_{18:1{\omega}9}$ (11.8 mol %), followed with $C_{16:0}$ (4.8 mol %) and $C_{18:0}$ (3.1 mol %). The TG fraction was resolved into 20 TG molecular species according to the partition number (PN) by reversed-phase (RP)-HPLC. The main TG species were $DT_{c2}$, $MDT_{c}$ and $D_{2}T_{c}$, of which amounts reached 63 mol % of total TG molecular species. The TG sample was fractionated into 11 fractions according to the number of double bond in the molecule by $Ag^{+}-HPLC$ and the species of $DT_{c2}$, $MDT_{c}$ and $D_{2}T_{c}$ were also eluted as main components. The TG species containing CTA showed unusual behaviours in the order of elution by HPLC ; first, TG moleular species of $DT_{c2}$ (D; dienoic acid, $T_{c}$; punicic acid, $T_{ci}$; ${\alpha}-eleostearic$ acid, M ; monoenoic acid, $S_{t}$; stearic acid) was eluted earlier than $Mt_{c2}$, although they have the same PN number of 40, and, secondly, the species of $DT_{ci2}$ with eight double bonds was eluted earlier than that of $D_2T_{ci}$ with seven double bonds. Intact TG of the seed oils of Momordica charantia contained mainly fatty acids such as $C_{18:3{\omega}9c,11t,13t}$ (57.7 mol %), $C_{18:1{\omega}9}$ (17.4 mol %), $C_{18:0}$ (12.3 mol %) and $C_{18:2{\omega}6}$ (10.6 mol %), and was classified into 13 fractions by RP-HPLC. The main TG species were as follows ; $MT_{ci2}$ [$(C_{18:1{\omega}9})(C_{18:3\;9c,11t,13t})_{2}$, 39.1 mol %] and $S_{t}T_{ci2}$ [$(C_{18:0})(C_{18:3\;9c,11t,13t})_2$, 33.9 mol %] comprising about 73 mol % of total TG species, accompanied by $DT_{ci2}$ [$(C_{18:2{\omega}6})(C_{18:3\;9c,11t,13t})_{2}$, 7.3 mol %], $D_{2}T_{ci}$ [$ (C_{18:2{\omega}6})_{2}(C_{18:3\;9c,11t,13t})$, 3.6 mol %] and $MDT_{ci}$ [$(C_{18:1{\omega}9})(C_{18:2{\omega}6})(C_{18:3\;9c,11t,13t})$, 3.5 mol %]. Simple TG species of $T_{ci3}$ [$(C_{18:3\;9c,11t,13t})_3]$ was present in a small amount of 1.4 mol %, but other simple TG species were not detected. The TG was also resolved into 11 fractions according to the number of double bond by $Ag^{+}-HPLC$, and the species were mainly occupied by $MT_{ci2}$ [$(C_{18:1{\omega}9})(C_{18:3\;9c,11t,13t})_{2}$, 39.4 mol %] and $S_tT-{ci2}$ [$(C_{18:0})(C_{18:3\;9c,11t,13t})_{2}$, 35.4 mol %] $DT_{ci2}$ species with eight double bonds was also developed faster than $D_2T_{ci}$ one with seven double bonds as indicated in the analysis of TG of the seed oils of T. kirilowii, and $MT_{ci2}$ species with cis, trans, trans-configurated double bond was eluted earlier than $MT_{c2}$ species with cis, trans, cis-configurated double bond. The main components of fatty acid in total TG fraction isolated from the seed oils of of Aleurites fordii were in the following order ; $C_{18:3\;9c,11t,13t}$ (81.2 mol %)> $C_{18:2{\omega}6}$ (8.5 mol %)> $C_{18:1{\omega}9}$ (5.4 mol %)$. With resolution of the TG by RP-HPLC, eight fractions such as $T_{ci3}$, $Dt_{ci2}$, $D_{2}T_{ci}$, $MT_{ci2}$, $PT_{ci2}$ (P; palmitic acid), $PMT_{ci}$, $PDT_{ci}$ and $S_{t}T_{ci2}$ ($S_{t}$; stearic acid) were isolated, respectively. TG species of $T_{ci3}$ [$(C_{18:3\;9c,11t,13t})_{3}$, 54.2 mol %], $DT_{ci2}$ [$(C_{18:2{\omega}6})(C_{18:3\;9c,11t,13t})_{2}$, 15.0 mol %] and $MT_{ci2}$ [$(C_{18:1{\omega}9})(C_{18:3 9c,11t,13t})_{2}$, 14.8 mol %] were present as main species.

Effect of Monosodium Glutamate and Temperature Change on the Content of Free Amino Acids in Kimchi (Monosodium Glutamate와 숙성온도 변화가 김치의 유리아미노산 함량에 미치는 영향)

  • 이예경;이명예;김순동
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.2
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    • pp.399-404
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    • 2004
  • This study was conducted to investigate the effect of monosodium glutamate (MSG) and fermentation methods (C-I; fermented for 5 days at 1$0^{\circ}C$ after 2$0^{\circ}C$ fermentation for 2 days, C-II; fermented for 7 days at 1$0^{\circ}C$, M-I; kimchi with MSG fermented for 5 days at 1$0^{\circ}C$ after 2$0^{\circ}C$ fermentation for 2 days, M-II; kimchi with MSG fermented for 7 days at 1$0^{\circ}C$ on fermentation and free amino acid content. Fermentation of M-I and M-II was slightly delayed compared to C-I and C-II. Total microbe of C-I and C-IIwere lower than those of M-I and M-II, and lactic acid bacteria of C-I and C-II were lower than those of M-I and M-II respectively. The major free amino acids were alanine, asparagine, homocystine and valine in C-I, especially, glutamic acid and ornithine were high in C-II. Homocystine, alanine, asparagine and valine in M-I, glutamic acid, alanine, hydroxyproline, asparagine, homocystine, ornithine and valine were the major free amino acid in M-II, respectively. The sour taste of M-I and M-II was lower than those of C-I and C-II, respectively, and the effect of delaying fermentation at 1$0^{\circ}C$ did not showed in the C-I and M-I. The crispy taste of the M-I and M-II was higher than those of C-I and C-II, which was the opposite results of sour taste. Palatable and overall taste of M-I and M-II were higher than those of C-I and C-II, respectively These results suggest that the MSG in kimchi affect not only increment of free amino acid content but also shelf-life and taste improvement, and continuous fermentation at 1$0^{\circ}C$ also enhance the content of free amino acid and shelf-life of kimchi.