• Korean Journal of Microbiology
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• v.48 no.2
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• pp.73-78
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• 2012

We previously reported that the truncated Cel44C-$Man26A_{P558}$ ${\beta}$-glycosyl hydrolase protein exhibits multifunctional activities, including cellulase, xylanase, and lichenase. DNA shuffling of the truncated Cel44C-$Man26A_{P558}$ enzyme was performed to enhance the enzymatic activity of the multifunctional ${\beta}$-glycosyl hydrolase. Two mutant enzymes, M2Cel44C-$Man26A_{P558}$ that carries one mutation (P438A) and M21Cel44C-$Man26A_{P558}$ that carries two mutations (A273T and P438A) were obtained. The enzymatic activity of the M21Cel44C-$Man26A_{P558}$ double mutant was lower than enzymatic activity of the single mutant (M2Cel44C-$Man26A_{P558}$). However, both mutants displayed the enhancements in their enzyme activities that were ${\approx}1.3$- to 2.2-fold higher than the original enzymatic activity in Cel44C-$Man26A_{P558}$. In particular, the mutant M2Cel44C-$Man26A_{P558}$ exhibited an approximate 1.5- to 2.2-fold increase in the cellulase, xylanase, and lichenase activities in comparison with the control (Cel44C-$Man26A_{P558}$). The optimum cellulase, linchenase, and xylanase activities of ${\beta}$-glycosyl hydrolase were observed at pH 7.0, pH 7.0 and pH 6.0, respectively. These results, therefore, suggest that the amino acid residue Ala438 plays important roles in the enhancement of the activity of multifunctional ${\beta}$-glycosyl hydrolase.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.482-487
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• 2009

The apple rootstock M.26 (Malus pumila) is very popular apple rootstock with semi-dwarf habit and the trees on M.26 produce high quality fruit at a young age. Since it is prone to poor prop ability to soil, however, young trees require staking in windy locations. The rolC gene was introduced into M.26 by Agrobacterium tumefaciens LBA4404 harboring pBI121 to obtain its transformants with dwarfism and enhanced rooting ability. One regenerated transgenic line was confirmed by polymerase chain reaction (PCR) analysis and Southern blot analysis of genomic DNA for the existence of rolC gene. The characteristics of transgenic line in vitro were not significantly different from non-transgenic line except for the active root formation and lateral root number. The rolC transgenic line showed reduced stem length and increased root number in vitro. Rooting ability was examined in the isolated greenhouse after mound layering. Compared to non-transgenic M.26, rolC transgenic line showed significantly higher rooting ability. The transgenic line did not show any other observable variation in shoot phenotype compared with non-transgenic line excepting increased branching

• Journal of Animal Science and Technology
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• v.46 no.6
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• pp.909-916
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• 2004

CCAAT/enhancer binding proteins(C/EBP) are a group of transcription factors expressed during preadipocyte differentiation. In the C/EBPs, C/EBPa plays an important role in lipid deposition and adipocyte differentiation. In this studies, we report the identification, characterization, and expression of a Hanwoo CIEBP$\alpha$ The Hanwoo C/EBP$\alpha$DNA includes a 1059 bp open reading frame encoding a protein of 353 amino acids. The CIEBPa amino acid sequences of the Hanwoo show strong conservation with the corresponding sequences reported in other species. The distribution of C/EBP$\alpha$ mRNA in various tissues of Hanwoo aged 12 months were investigated using Northern blotting analysis. The highest expression was detected in adipose tissue and more lower expression was detected in colon and lung. We also identified expression of C/EBPa mRNA in Hanwoo sirloin and adipose tissue aged 12, 26, and 30 months by real-time RT-PCR. The higest expression were detected at 26 months in the sirloin and at 12 and 26 months in the adipose tissue.

• Journal of Aquaculture
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• v.15 no.3
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• pp.131-138
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• 2002

Fed on commercial flounder diet at 20, 23 and $26^{\circ}C$ in semiclosed culture system for 32 weeks, the juvenile red drum (1.2 g) showed linear increase in daily feeding rate (DFR), growth (g/fish) and specific growth rate (SGR) with increasing temperature. In the second experimental series, the young red drum (214 g), cultured at densities of 2.16, 4.24 and 6.40 kg/$m^3$ in flow-through tanks at water temperatures from 12.3 to $27.2^{\circ}C$ for 25 weeks, grew faster at the stocking density of 2.16 kg/$m^3$ than at the densities of 4.24 and 6.40 kg/$m^3$ the difference in growth observed at the stocking densities of 4.24 and 6.40 kg/$m^3$ was not significant. The DFR and SGR were also significantly higher for the density group of 2.16 kg/$m^3$. Briefly, growth of the red drum increased with increasing tested range of temperature and was also faster with decreasing stocking density. However, the total growth (g/tank) increased with increasing stocking density.

• Korean Journal of Agricultural and Forest Meteorology
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• v.18 no.3
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• pp.151-161
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• 2016

This study was conducted to find out influence of dormancy level and carbon concentration on freezing hardiness in bourse shoot of 'Fuji' apple tree. Bourse shoot of 'Fuji' adult apple tree grafted on M.26 and M.9 rootstocks were used as experimental materials. Dormancy levels of bourse shoot were categorized according to the periods as follows the internal dormancy (late January), the early days after internal dormancy breaking (early February), the late days after internal dormancy breaking (late February), the bud break (late March), and the full bloom (late April). Chilling temperatures with bourse shoot were ranged from 0 to $-40^{\circ}C$. Also, the freezing hardiness according to carbon concentrations were investigated on 'Fuji'/M.9 apple tree that defoliated severely by Marssonina blotch (defoliation) and that of below the average 20 cm in shoot length through heavy crop load (weakness). Results showed that freezing hardiness of bourse shoot may become weaker after internal dormancy breaking. There was no differences in the carbon concentration of bourse shoot of 'Fuji' apple tree grafted on M.9 and M.26, so may be resulted in no difference in freezing hardiness both of bourse shoot grafted on M.9 and M.26 rootstock. Carbon concentration in bourse shoots with weakness and C/N ratio in bourse shoots with defoliation were lower than that of healthy. It may be shown that the freezing hardiness of defoliation and weakness were weaker than that of healthy.

• Proceedings of the Korean Powder Metallurgy Institute Conference
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• 1994.10b
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• pp.26-26
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• 1994

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.26 no.6
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• pp.441-445
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• 2013

ZnO nanowires on the a-, c- and m-plane oriented 4H-SiC substrates were grown by using a high temperature tube furnace. Ti/Au electrodes were deposited on ZnO nanowires and a-, c- and m-plane 4H-SiC substrates, respectively. The shape and density of the ZnO nanowires were investigated by field emission scanning electron microscope. It was found that the growth direction of nanowires depends strongly on growth parameters such as growth temperature and pressure. In this work, The sensitivity of nanowires formed a-, c- and m-plane oriented 4H-SiC gas sensor was measured at $300^{\circ}C$ with CO gas concentration of 80%. The nanowires grown on a-plane oriented 4H-SiC show improved sensing performance than those on c- and m-plane oriented 4H-SiC due to the increased density of nanowire on a-plane 4H-SiC.

• Korean Journal of Remote Sensing
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• v.5 no.1
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• pp.57-67
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• 1989

This study on the southern coastal waters of Korea has been made by analysis of NOAA image and oceanographic observation data from October 1987 to August 1988. The results obtained from the study are as follow: Horizontal distributions of water temperature in different layers in winter ranged from 6.07 to 18.62$^{\circ}C$ at 0m layer, 6.02 to 18.54$^{\circ}C$ at 30m layer and 7.19 to 18.69$^{\circ}C$ at 50m layer. Consequently its vertical distribution showed homogeneity. Horizontal water temperature gradients were 0.28$^{\circ}C$/mile between the coastal waters and Tsushima warm waters. In summer, its horizontal distribution varied from 19.37 to 29.92$^{\circ}C$ at 0m layer, 13.26 to 27.11$^{\circ}C$ at 30m layer and 7.36 to 26.6$0^{\circ}C$ at 50m layer, and its vertical profile showed stratified structure. Vertical water temperature gradients were 0.44$^{\circ}C$/m between 30 and 50m layers. It was remarkable that distribution of southern coastal water system analysed by NOAA image coincided with relatively the oceanographic observation data but SST from NOAA image seemed to be 2-4$^{\circ}C$ lower in winter and 4-6$^{\circ}C$ lower in summer than the oceanographic data.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.3
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• pp.991-994
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• 2012

Objective: VEGF-C has recently been identified as a key molecule which is involved in tumor lymphangiogenesis. The aim of this research was to investigate the role of PARP-1 inhibition in the regulation of VEGF-C expression in CT26 cells. Methods: CT26 cells were treated with or without the PARP-1 inhibitor 5-aminoisoquinolinone (5-AIQ). The expression of PARP-1, NF-kB, and VEGF-C proteins in CT26 cells was measured by Western blot analysis and the VEGF-C mRNA level was determined by reverse transcription polymerase chain reaction (RT-PCR). CT26-secreted VEGF-C was detected by enzyme-linked immunosorbent assay (ELISA). Results: The results of Western blot analysis showed that the expression levels of PARP-1, NF-kB, and VEGF-C were reduced in 5-AIQ treated CT26 cells and the levels of VEGF-C mRNA in 5-AIQ treated CT26 were significantly lower than t in 5-AIQ-untreated cells (P<0.05). The concentrations of CT26-secreted VEGF-C were also dramatically decreased (P<0.05). Conclusion: Here, we provide evidence for the first time that PARP-1 inhibition dramatically reduces VEGF-C expression via the nuclear factor NF-kB signaling pathway. We therefore propose that PARP-1 inhibition has an anti-lymphangiogenic effect and may contribute to the prevention of metastatic dissemination via the lymphatic system.

• Journal of The Korean Astronomical Society
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• v.13 no.1
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• pp.15-26
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• 1980

The BV-photographic photometry was made for 1714 stars (V<19.5) in NGC 6752. The C-M diagram of this cluster shows an unusually extended blue horizontal branch $(V=13.5{\sim}17.8)$ with a wide gap $(V=16{\sim}16.7)$ and the well defined giant branch with gaps at V=13.85 and 16.2. The turnoff point is defined at $V=17.25{\pm}0.15$ and (B-V) = $0.46{\pm}0.02$. If we take $V_{HB}=13.85$ for NGC 6752, then ${\Delta}V=2.80,\;(B-V)_{0,g}=0.76\;and\;{\Delta}V_{TO}=3.40$ and the chemical abundance is estimated to be [Fe/H]=-1.67 or $Z=4.3{\times}10^{-4}\;and\;Y=0.26$. Some other physical parameters of this cluster are derived and compared with those for the well observed clusters M 3, M 13, M 15 and M 92.