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Physicochemical Characteristics of Gamma Irradiated Changran Jeotkal during Storage at $10^{\circ}C$ (감마선 조사기술을 이용하여 제조된 양념창란젓갈의 이화학적 품질특성)

  • Lee, Na-Young;Jo, Cheo-Run;Lee, Won-Dong;Kim, Jae-Hyun;Byun, Myung-Woo
    • Korean Journal of Food Science and Technology
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    • v.35 no.6
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    • pp.1129-1134
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    • 2003
  • Changran jeotkal, a Korean traditional fermented seafood, was prepared as a pilot scale using a commercial method and irradiated at 0, 2.5, 5.0 and 10 kGy by gamma ray to investigate possibilities for further industrial application. To see the effectiveness and rapid industrialization, hot pepper powder was irradiated at 10 kGy and manufactured the changran jeotkal (HP-10 kGy) as same method since the hot pepper powder was approved legally for gamma irradiation in Korea. The content of volatile basic nitrogen and amino nitrogen was significantly reduced by gamma irradiation in all storage periods. Amino nitrogen contents of 0, 2.5, 5.0, 10 kGy and HP-10 kGy were 98.9, 98.5, 92.4, 88.0 and 93.1mg%, respectively after 12 week of storage at $10^{\circ}C$. In total, 8 kinds of biogenic amines were found from the samples, and the contents in the gamma irradiated changran Jeotkal were lower than those of the control during storage. The sample of HP-10 kGy showed similar physicochemical characteristics to the sample irradiated as $2.5{\sim}5.0\;kGy$. Results indicated that gamma irradiation of fermented seafood products such as seasoned changran jeotkal improved quality stability, thus, we recommend gamma irradiation for industrial application.

Characteristics of Growth and Germination of Salicornia herbacea L. for the Soil salinity and Manure Condition (토양염분.시비 조건에 따른 퉁퉁마디 생장 및 발아 특성)

  • Jo, Yeong-Cheol;Lee, Kyeong-Sik;Chon, Song-Mi;Byun, Do-Seung
    • Korean Journal of Medicinal Crop Science
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    • v.10 no.2
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    • pp.100-108
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    • 2002
  • This experiment was carried out to investigate the cultivation condition of soil salinity, manure and to find out the cultivation capability of Salicornia herbacea. The optimal growth condition of soil salinity was between 1% and 4%. The growth of groups for fertilization was significantly better than control group. Salicornia herbacea grew very up from 7 kg/l0a to 9 kg/l0a for N, 12 kg/l0a for P and the K-fertilizer group was better than control group but there was not significantly different among the conditions of K-concentration. The germination was good from $0{\textperthousand}\;to\;5{\textperthousand}$ for salinity, from $20^{\circ}C\;to\;30^{\circ}C$ for temperature. On the experiment cultivation, the production by hill seeding was $5.40{\sim}5.90 kg/m^2$ and was significantly higher than the yield by broadcast sowing which was $4.01{\sim}4.20 kg/m^2$. The production by hill seeding was 2.7 times than natural production and the production by broadcast sowing 1.9 times.

The Analysis of Learner's Motivation and Satisfaction with 3D Printing in Science Classroom (3D 프린팅을 활용한 과학 수업에서 학습자의 동기와 만족감 분석)

  • Byun, Moon-Kyoung;Jo, Jun-Ho;Cho, Moon-Heum
    • Journal of The Korean Association For Science Education
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    • v.35 no.5
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    • pp.877-884
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    • 2015
  • Technology is an important means to enhance students' understanding about scientific concepts. In particular, newly introduced 3D printing technology has great potential to help students learn scientific concepts better. 3D printing is a process for a creating physical object with a three dimensional model. In this study, we explored two types of learners' (students vs. adults) motivation and satisfaction with 3D printing technology. With regard to motivation, student learners showed higher task value, self-efficacy for learning, and satisfaction than adult learners. The result implied that 3D printing technology is more effective to student learners than adult learners. In addition, for adult learner group, negative relationship between technology and satisfaction was found. Therefore, support for reducing the technology anxiety for adult learners is necessary. Further discussions are provided for the research and application of 3D printing technology in science classroom.

Polycyclic Aromatic Hydrocarbons Hazard Assessment of Shellfish due to Oil Spill Accidents (유류 유출 사고 해역에 서식하는 패류의 다환방향족탄화수소(PAHs) 위해도 평가)

  • Kim, Poong-Ho;Kim, Min-Jeong;Jo, Mi-Ra;Lee, Doo-Seog;Song, Ki-Cheol;Byun, Han-Seok;Cho, Kee-Chae;Park, Kwang-Jae;Jun, Je-Cheon;Yoon, Ho-Dong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.43 no.3
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    • pp.211-216
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    • 2010
  • On 7 December 2007, about 12,547 kL of crude oil spilled from the Hong Kong registered tanker Hebei Spirit along the west coast of the Republic of Korea, including Taean-gun, Chungcheongnamdo Province. This study evaluated the safety of seafood collected from the coastal area polluted by the crude oil. The range of total polycyclic aromatic hydrocarbons (${\sum}PAHs$) at 22 stations was 3.9-37.1 ng/g. The concentration of ${\sum}PAHs$ was higher in oysters, Crassoatrea gias, than that in short-necked clams, Ruditapes philippinarum. Benzo(a)pyrene, a highly toxic PAH, ranged from 0.07-1.47 ng/g, which did not exceed the European Union regulatory limit for benzo(a)pyrene. The toxicity equivalent of benzo(a)pyrene in oysters and short-necked clams was 0.49-1.70 and 0.09-1.01 ng/g, respectively. The estimated life time cancer risk was very low, i.e., $1.31{\times}10^{-8}$ for the oysters and $6.9{\times}10^{-9}$ for the short-necked clams. The body burden of PAHs in bivalves originated mostly from petroleum contamination, but the levels was not sufficiently high to harm human health.

Microbiological and Mutagenical Safety Evaluation of Gamma Irradiated Ready-to-Eat Foods of Animal Origin (즉석식품 제조를 위한 육가공제품의 감마선 조사에 따른 미생물 및 유전독성학적 안전성 평가)

  • Lee Na-Young;Jo Cheorun;Kang Ho-Jin;Hong Sang-Pill;Kim Young-Ho;Lee Kyong-Haeng;Byun Myung-Woo
    • Food Science of Animal Resources
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    • v.25 no.1
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    • pp.13-19
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    • 2005
  • The radio-sensitivity of pathogens and the effect of irradiation on microbiologican safety and mutagenicity of meat products such as seasoned and cooked beef and ham were investigated. Samples were radiation-sterilized and inoculated at 10/sup 7/ cfu/g with each of the four pathogens including Salmonella typhimurium, Escherichia coli, Staphylcoccus aureus, and Listeria ivanovii. No viable cells of pathogens were observed in the sample irradiated with 3 kGy. The D/sub 10/ value of inoculated pathogens in seasoned and cooked beef and ham were 0.24∼0.48 and 0.39∼0.45, respectively. Results of Ames test performed with non-irradiated and irradiated seasoned and cooked beef and ham were both negative at the level of 625, 1,250, 2,500, 50,000, and! 10,000 ㎍ sample/plate, respectively. Results indicate that low dose (2∼3 kGy) irradiation is effective to ensure safety for seasoned and cooked beef and ham with toxicological wholesomeness.

Inhibition of Photooxidation by Breakdown of ChlorophyII in Oil Model System Using Gamma Irradiation (감마선 조사된 유지 model system의 chlorophyII 제거 및 광산화 억제)

  • Lee, Kyong-Haeng;Jo, Cheo-Run;Yook, Hong-Sun;Byun, Myung-Woo
    • Korean Journal of Food Science and Technology
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    • v.33 no.6
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    • pp.693-699
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    • 2001
  • ChlorophyII destruction by irradiation in linoleic acid in methanol system was studied. ChlorophyII b standard (3 ppm) was added into methanol solution containing 1% of linoleic acid, and the sample was irradiated up to 20 kGy with or without nitrogen gas-bubbling. The content of chlorophyII b was analyzed by HPLC, and Hunter color value and UV-visible spectra were analyzed during 6 hr of photooxidation. The content of chlorophyll was reduced by irradiation and completely destroyed at 2.517 kGy. The model system with nitrogen gas-bubbling did not develop the lipid oxidation after irradiation or photooxidation at 20 kGy UV-visible spectra showed decreasing values of optical density by increase of irradiation doses, supporting the destruction of chlorophyII b. The results indicate that irradiation technology can be applied to reduce or eliminate the residual chlorophyII and to prolong the shelf-life of oil products.

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Recovery of Protein Hydrolysate from Hoki (Johnius belengeri) Frame with Tuna Pyloric Caeca Crude Enzyme and Its Functionalities (참치 유래 조효소를 이용한 민태(Johnius belengeri) Frame으로부터 단백질 가수분해물의 회수 및 그 기능성)

  • Jeon, You-Jin;Lee, Byoung-Jo;Byun, Hee-Guk;Kim, Jong-Bae;Kim, Se-Kwon
    • Applied Biological Chemistry
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    • v.42 no.1
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    • pp.49-57
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    • 1999
  • Enzymatic hydrolysis with tuna pyloric caeca crude enzyme(TPCCE) was performed to recover a protein hydrolysate from hoki frame, fish processing by-product. Optimum hydrolytic conditions were pH 10.0, temperature $50^{\circ}C$, and incubation time 12 hrs, and then the degree of hydrolysis was about 60%. The yield of the hydrolysate from hoki frame by enzymatic hydrolysis was approximately 77% on a dry weight basis. The prepared protein hydrolysates were also fractionated through a series of 30, 10, 5 and 1 kDa molecular weight cut-off (MWCO) membranes in order to investigate the effect of their functionalities according to the difference of their molecular size. As the result of studying functionalities of the hydrolysates, 1 K hydrolysate showed the highest solubility over all pHs, and 30 and 10 K hydrolysate showed more excellent emulsifying property and whippability than the other hydrolysates.

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Microbiological Safety of Minimally Processed White Radish in Modified Atmosphere Packaging Combined with Irradiation Treatment (가스치환포장과 감마선조사 병용 처리한 최소가공 무의 미생물학적 안전성)

  • Kim, Jae-Kyung;Jo, Cheo-Run;Kim, Hyun-Ju;Lee, Ju-Woon;Hwang, Han-Joon;Byun, Myung-Woo
    • Korean Journal of Food Science and Technology
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    • v.37 no.1
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    • pp.11-14
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    • 2005
  • White radish was minimally processed, packed with air, $CO_{2}$ (100%), and $CO_{2}/N_{2}$ (25/75%), and irradiated at 0, 1, and 2 kGy, and its microbiological quality and pH were investigated during storage for 2 weeks at $4^{\circ}C$. Irradiation significantly reduced total aerobic, coli-form, and lactic acid bacteria counts. Modified atmosphere packaging (MAP) enhanced microorganism control during storage. Acidity decreased by MAP but was restored during storage, Irradiation did not affect sample pH. Results show irradiation at 2 kGy combined with MAP can enhance microbiological safety and quality of minimally processed radish.

Bone reaction to bovine hydroxyapatite grafted in the mandibular defects of beagle dogs. (성견의 하악 골 결손부에 이식한 생체 유래 골 이식재 (OCS-B)에 대한 치조골의 반응)

  • Byun, Yu-Kyung;Park, Jun-Beom;Kim, Tea-Il;Seol, Yang-Jo;Lee, Yong-Moo;Ku, Young;Lee, Hye-Ja;Chung, Chong-Pyoung;Han, Soo-Boo;Rhyu, In-Chul
    • Journal of Periodontal and Implant Science
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    • v.36 no.1
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    • pp.39-49
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    • 2006
  • 1. 목 적 이 연구의 목적은 성견의 하악 골 결손부에 이식한 생체 유래 골 이식재에 대한 치조골의 반응을 알아보는 것이다. 2. 연구방법 및 재료 생후 1년 이상 된 성견 4마리의 하악 제2소구치 및 제 4 소구치를 발거하고 발치와에 금원심 폭경 8mm, 협설 폭경 5mm, 치조정에서의 깊이 6mm인 결손부를 형성하였다. 4주간의 자연 치유 후 판막을 형성하여 결손부의 크기를 확인하였다. 각각의 결손부 크기가 일정하도록 수정한 후 '이식재+차폐막'군에는 OCS-B을 이식하고 Bio-gide을 차단막으로 사용한 후 봉합하고 '이식재군'은 OCS-B 이식 후 차폐막 없이 봉합하였으며 '비이식'군은 아무런 처치없이 일차봉합하였다. 수술 4, 6주에 실험동물을 각각 희생시켜 실험부위를 적출하고 비탈회 연마 표본을 제작하여 골 치유 양성을 조직학적 및 조직계측학적으로 관찰하였다. 3. 연구결과 이식재 비이식군 및 이식군 모돼서 별다른 부작용없이 잘 치유되었다. 세 실험군 모두에서 술후 4주에 비교하여 술 후 6주에서의 결손부 산생골 형성량이 증가하였다. 술후 4주 소견에서 비이식군은 결손부 주변부위에서 골이 생성되어 나오는 양상을 보였으며 이식군은 이식재 주변으로 골침착 시작되는 것을 관찰할 수 있었다. 술후 6주 소견에서 비이식군은 결손부 경계부로부터의 지속적인 골 생성을 관찰할 수 있었으며 이식군은 이식재 주변으로 침착된 골의 양이 많아지고 신생골이 가교를 형성하는 것을 관찰할 수 있었다. 4. 결 론 차폐막 유무와 상관없이 OCS-B는 염증반응을 전혀 일으키지 않았으며 우수한 골 전도성을 보였다. 또한 결손부의 형태를 잘 유지하여 골재생을 위한 공간을 확보할 수 있었다. 이는 OCS-B가 골이식재로서의 필요조건을 갖추었음을 확인한 결과이며 보다 장기적인 관찰에서 OCS-B의 흡수 가능성을 확인하는 것이 필요할 것으로 보인다.

Effects of a Herbal Composition (HemoHIM) on the Activation of Dendritic Cells (생약복합조성물(HemoHIM)의 수지상세포 활성화 효과)

  • Shin, Sung-Hae;Kim, Do-Soon;Kim, Sung-Ho;Jo, Sung-Kee;Byun, Mung-Woo;Yee, Sung-Tae
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.10
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    • pp.1322-1328
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    • 2006
  • In our previous study, a novel herb mixture (HIM-I) of Angelica gigas radix, Cnidium officinale rhizoma, and Paeonia japonica radix was developed to protect= the intestinal and immune systems and to promote their recovery from radiation damage. A new herbal composition (HemoHIM) with the high immune modulating activity was developed from HIM-I. In the present study, we examined the effects of HemoHIM on the maturation process of murine bone marrow (BM)-derived dendritic cells (DC). BM cells were cultured in the presence of iL-4 and GM-CSF and the generated immature DC were stimulated with HemoHIM for 24 hours. HemoHIM significantly enhanced the expression of co-stimulatory molecules, CD80 and CD86, especially. The activation capacity of HemoHIM-treated DC was significantly higher than that of immature DC, as analyzed by IL-2 and $IFN-\gamma$ production and proliferation of the responding T cells in the co-culture with allogeneic T cells. The antigen-presenting capacity of HemoHIN-treated DC was also increased by the co-culture with OVA-specific T cells (HS-1), as analyzed by IL-2 and $IFN-\gamma$ production and the proliferation. These results indicate that HemoHIM causes the maturation and ;Activation of DC, which may be a part of mechanisms of immunomodulation by HemoHIM.