• Bulletin of the Korean Chemical Society
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• v.29 no.12
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• pp.2365-2367
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• 2008

• Journal of Microbiology
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• v.43 no.4
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• pp.375-380
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• 2005

Autonomous ultradian metabolic oscillation (T$\simeq$50 min) was detected in an aerobic chemostat culture of Saccharomyces cerevisiae. A pulse injection of GSH (a reduced form of glutathione) into the culture induced a perturbation in metabolic oscillation, with respiratory inhibition caused by $H_2S$ burst pro-duction. As the production of $H_2S$ in the culture was controlled by different amino acids, we attempted to characterize the effects of GSH on amino acid metabolism, particularly with regard to branched chain and sulfur-containing amino acids. During stable metabolic oscillation, concentrations of intra-cellular glutamate, aspartate, threonine, valine, leucine, isoleucine, and cysteine were observed to oscil-late with the same periods of dissolved $O_2$ oscillation, although the oscillation amplitudes and maximal phases were shown to differ. The methionine concentration was stably maintained at 0.05 mM. When GSH (100 $\mu$M) was injected into the culture, cellular levels of branched chain amino acids increased dramatically with continuous $H_2S$production, whereas the cysteine and methionine concentrations were noticeably reduced. These results indicate that GSH-dependent perturbation occurs as the result of the promotion of branched chain amino acid synthesis and an attenuation of cysteine and methionine synthesis, both of which activate the generation of $H_2S$. In a low sulfate medium containing 2.5 mM sulfate, the GSH injections did not result in perturbations of dissolved $O_2$ NAD(P)H redox oscillations without burst $H_2S$ production. This suggests that GSH-dependent perturbation is intimately linked with the metabolism of branched-chain amino acids and $H_2S$ generation, rather than with direct GSH-GSSG redox control.

• The Korean Journal of Physiology
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• v.29 no.1
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• pp.103-111
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• 1995

Intracellular recordings were obtained from Purkinje cells in rat cerebellar slices maintained in vitro. Adding tetrodotoxin to the superfusion solution produced a typical pattern of repetitive burst firing consisting of a cluster of action potentials followed by a long hyperpolarization. TTX-induced oscillatory activity was not due to modulation of membrane potential although underlying mechanisms for maintenance of oscillatory activity were influenced by membrane voltage. The mechanism of TTX-induced oscillation was not related to the presence or amplitude of $I_h$ and could still induce the oscillatory activity after blockade of $I_h$ by cesium. The result from an experiment in which QX-314 was injected intracellularly strongly suggested that TTX-induced oscillatory firing activity was due to blockade of post-synaptic $Na^{+}$ currents intrinsic to PCs.

• Publications of The Korean Astronomical Society
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• v.30 no.2
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• pp.57-58
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• 2015

In this study, we present geometrical and kinematical analysis of Moreton wave observed in 2012 June 3rd and July 6th, recorded in H-${\alpha}$ images of Global Oscillation Network Group (GONG) archive. These large-scale waves exhibit different features compared to each other. The observed wave of June 3rd has angular span of about $70^{\circ}$ with a diffuse wave front associated to NOAA active region 11496. It was found that the propagating speed of the wave at 17:53 UT is about $931{\pm}80km/s$. The broadness nature of this Moreton wave can be interpreted as the vertical extension of the wave over the chromosphere. On the other hand, the wave of July 6th associated with X1.1 class are that occurred at 23:01 UT in AR NOAA11515. From the kinematical analysis, the wave propagated with the initial velocity of about $994{\pm}70km/s$ which is in agreement with the speed of coronal shock derived from type II radio burst, v ~ 1100 km/s. These two identified waves add the inventory of the large-scale waves observed in 24th Solar Cycle.