• Korean Journal of Medicinal Crop Science
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• v.10 no.5
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• pp.344-352
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• 2002

This study was conducted to determine the optimum planting dates and density of one year old Bupleurum falcatum L. to improve its quality. Furthermore, the effect of cultural methods and plant growth regulators$(GA_3,\;IAA,\;Kinetin)$ on the quality of B. falcatum were also investigated. In this study, Jeongseon cultivar collected in Korea and Mishima cultivar introduced from Japan were used. Some of the results obtained are as follows. Jeongseon cultivar showed less stem branches and shoot weight compared to Mishima. However, Jeongseon cultivar showed tall plant height, high root fresh and dry weight, and high levels of SSa and TSS, but low SSc content than that of Mishima. Although fresh and dried root weight of both cultivars were not affected by plant growth regulators treatment. compared with the untreated one, increasement of TSS content were made by $GA_3$ 100 ppm, IAA 50 ppm or 10 ppm and kinetin 10 ppm treatment in Jeongseon cultivar, and by $GA_3$ 10 ppm and IAA 100 ppm or 10ppm treatment in Mishima cultivar applied on June 1.

• Korean Journal of Medicinal Crop Science
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• v.5 no.1
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• pp.62-66
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• 1997

This experiment was conducted to evaluated the effect of choline salt of maleichydrazide (C-MH) application on growth and root yield components of Bupleurum falcatum L. Flowering was delayed by C-MH application $17{\sim}35$ days. Twice application was more effective to delay flowering. Application of C-MH 2,000ppm at 30cm to 40cm of plant height reduced plant height 15cm, but increaced highly branches and the growth of root. The dried root yield per 10a was 27% higher in twice application of C-MH 2,000ppm than control plot.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.spc1
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• pp.123-144
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• 1996

This review deals briefly with the various medicinal components(mainly saikosaponins), their biological activities and the variation of their contents by different cultivation environment and plant parts in Bupleurum species. Bupleuri radix, a crude drug, is the root of Bupleurum falcatum L. (Korea, Japan), B. chinense(China), and their related species (Umbelliferae). There are over 120 species in Bupleurum genus throughout world, mainly Asian area, and over 5 species in Korea, investigated up to now. These plants contain many physiological active compounds and the principal components are saikosaponins. Major activities of this crude drug and saikosaponins are the anti-inflammatory and antihepatotoxic activities. Saikosaponins and their derivatives in Bupleurum spp. have been chemically studied, isolated and identified over 70 compounds in over 50 species. Other components, physiologically active ones, also have been investigated, which are the groups of lignan, flavonoid, essential oil, polyacetylene, polysaccharide, etc. Saikosaponins belong to the group of triterpenoid saponin chemotaxonomically and occur the accumulation and turnover in plant tissues through secondary metabolism, mevalonic acid pathway. The contents and kinds of saikosaponins and other components in Bupleurum spp. plants are various due to different species and growing environments, as the plant growth characters and yield are various. Most of medicinal plants as well as Bupleurum species are very useful as agricultural products and traditional medicines, and also are very valuable as genetic resources and natural products. So we need to collect, evaluate, preserve, and utilize various medicinal plants, and also to under-stand secondary metabolism and improve the breeding and cultivation techniques for the safe production of crude drugs with high quality and yielding.

• Korean Journal of Medicinal Crop Science
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• v.2 no.1
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• pp.32-37
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• 1994

This experiment was conducted to obtain the basic information for the increase ofgermination percent by the seed diameter, specific gravity, different grain filling period and stratification in Bupleurum falcatum seeds. The seed size of B.falcatum ranged from 2.0 to 3.9mm in diameter, The large seed showed the higher germinability, The optimum specific gravity of salt selection for seed screening was 1.05 resulting in screening out seeds with high germinability over 70%.The seeds needed grain filling period for 60 days or longer after flowering to keep high germinability The optimum period of stratification treatment to promote seed germination was about 80 days.

• Korean Journal of Medicinal Crop Science
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• v.5 no.4
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• pp.318-324
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• 1997

When Bupleurum falcatum field was irrigated three times with 20mm at intervals of 10days after sowing, establishment was inproved (64%) and root yield increased to 58kg/10a. And in early growing stage, 30mm irrigation six times at intervals of 10days increased the number of harvested plants per square meter and resulted in yield increase by 26% over yield from natural plot. 20mm irrigation in sowing time at intervals of 10days and 30mm irrigation in early growing stage at intervals of 10days kept adequate soil moisture content (soil moisture tension: $0.39{\sim}0.49bar)$ and resulted in better establishment, growth and yield of Buleurum falcatum.

• KSBB Journal
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• v.10 no.4
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• pp.435-440
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• 1995

During culture the effect of polyamine on hairy root of Buplerum falcatum by infection of Agrobacterium rhizogenes was studied. The fresh and dry weight of hairy root which cultured for 3months in MS medium increased about 7-fold when spermine at 10${\mu}$M, 100${\mu}$M was treated. After suspension culture of B. falcatum in MS medium containing putrescine(10${\mu}$M, 100${\mu}$M) or spermine(10${\mu}$M), the contents of endogenous polyamine (putresclne, spermidine, spermine) was higher than that of control. The ${\beta}$-glucan synthetase II activity by polyamine treatment was increased： especially spermidine (100${\mu}$M) and spermine(100${\mu}$M) stimulated it by about 180% and 220% respectively. These results suggest a possible role of polyamine as growth regulator in B. falcatum hairy root cultures.

• Korean Journal of Medicinal Crop Science
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• v.2 no.3
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• pp.193-197
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• 1994

Bupleurum falcatum was cultivated in plots of different soil textures, which were sandy loam(SL), loam(L), and clay loam(CL). The growth charafters of shoot and root parts in SL and L plots were better than CL one, and root yield was the most excellent in SL one. But the contents of total saikosaponins and methanol extract in its roots were the highest in CL plot. In any soil textures, one and two year old plants showed almost the same results in growth characters and saikosaponins contents. The growth and root yield of two year old plants were higher than those of one year old ones, but the contents of methanol extract and saikosaponins were lower. Flant characters were highly negative-correlated with the contents of saikosaponins and methanol extract.

• Korean Journal of Plant Tissue Culture
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• v.21 no.1
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• pp.41-46
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• 1994

Calli were induced from leaf explants of B.falcatum, and selected cell clumps of the calli (900-1, 000${\mu}{\textrm}{m}$) were cultured on MS medium supplemented with 0.1, 0.5, 1.0 or 2.0 mg/L 2, 4-D for 7 days, respectively: The clumps were subsequently transferred onto MS basal medium and subcultured for four weeks. In order to investigate the effect of 2, 4-D pretreatment, the selected clumps were cultured on MS medium supplemented with 0.1 mg/L 2, 4-D for 24, 48, 72, 96, 120 or 144 hours and then transferred to liquid MS basal medium, wherein they were cultured for 4 weeks. Histological observation showed that root initial cells were developed from cells on the surface of clumps or from cells in the inner region. Clumps on the basal medium produced mot within 5 days of culture. The rate of prutruding time was inversely proportional to the concentration of 2, 4-D. The number of adventitious roots per clump preheated with 0.1 mg/L 2, 4-D was an average of 5.2, which was the highest level. On MS medium as control, the clumps formed 3.3 adventitious roots each. As tile concentration of 2, 4-D increased, the number of adventitious roots were declined accordingly: The number of adventitious roots as the period of pretreatment increased upto 120 h.

• Korean Journal of Medicinal Crop Science
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• v.6 no.2
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• pp.154-159
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• 1998

This experiment was conducted to investigate the effects of plant age on germination and distribution of seed specific gravity and to find a reason of low germination rate in seeds harvested from young plants, which could be valuable information for the improvement of seed germination in Bupleurum falcatum L. The germination rates of Korean native cultivar were 35% and 4% higher in 1-year-and 2-year-old plants, respectively, than Misshimasaiko (三鳥柴胡) and showed no significant difference between 1- and 2-year-old plants. However, in Misshimasaiko, seed germination rates of 1-year-old plants were $22.7{\sim}23.0%$ lower than those of $2{\sim}3-year-old$ plants. The relative frequency of seeds over 1.00 specific gravity in Misshimasaiko was $33{\sim}34%$ higher in $2{\sim}3-year-old$ plants compared to 52% of 1-year-old plant, but no difference was found between plant ages in Korean native cultivar. Considering varietal difference, the germination rate of seeds with the same specific gravity was not affected by plant age in Korean native cultivar, but in Misshimasaiko it was greatly different between 1-year-old $(5.0{\sim}17.5%)$ and $2{\sim}3-year-old$ plants $(22.5{\sim}55.0)%$. From these results, it was assumed that differences in germination rate among seeds from different plant ages were caused not only by seed specific gravity but also by some other factors.

• Current Research on Agriculture and Life Sciences
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• v.9
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• pp.115-125
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• 1991

The medicinal plants of 33 species with symptoms of mosaic, necrosis, line pattern, malformation (fine leaf), stripe, and ring spot infected by cucumber mosaic virus spontaneously, were collected in Korea and Japan. Among them, 2 mosaic diseases of portulaca oleracea L. and Celocia argentea L., 2 necrotic mosaic diseases of Aristolochia debilis Sieb. and Tetragonia exponsa Murr., 6 mottle diseases of Cassia torosa Cav., Bupleurum falcantum L., Angelica acutioloba Kitagawa, A. keiskei Koidz., Peucedanum japonicum Thunb., and Foeniculum vulgare Mill., and ringspot disease of Basella rubra L., were named newly in these studies.