• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.42 no.2
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• pp.27-34
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• 2010

To obtain the basic information for the use of bamboo, the anatomical characteristics of moso bamboo shoots, and 20-day-old, 60-day-old, one-year-old, and two-year-old moso bamboo were observed by using polarizing microscope. The cross section showed that the bamboo shoots consists of the early stage of cell formation, atactostele, parenchyma, and bundle sheath was formed in 20-day-old moso bamboo, and all cells completely formed in 60-day-old moso bamboo. The tissues in one-year-old moso bamboo appeared to be completely matured. On the tangential surface, no atactostele was observed in the bamboo shoot, but metaxylem, parenchyma, and bundle sheath were found. atactostele and parenchyma appeared to be formed between 20 and 60 days. All tissues and cells were totally formed and the cell wall of parenchyma were completely matured after one year. The metaxylem in bamboo shoots was observed to have pits, which means that it could be the tissues first formed of all bamboo cells.

• Asian-Australasian Journal of Animal Sciences
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• v.6 no.2
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• pp.265-270
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• 1993

Ammonia and/or sulphur dioxide treated and untreated wheat leaf sheaths were compared for cell wall digestion by incubation with rumen liquor for 24 and 48 hours. Scanning electron microscope (SEM) was used to study the relative rate and extent of cell wall digestion. Treated wheat straw leaf sheaths were distorted, with more distortion observed in ammonia and sulphur dioxide combined treatment than any other treatment. Rumen liquor digestion for 24 hours of untreated leaf sheath showed disrupted phloem, partially ruptured parenchyma and vascular tissues and further partially distorted inner bundle sheaths and vascular bundle after 48 hours incubation. Sulphurated leaf sheaths showed extensive degraded parenchyma and sclerenchyma material in 24 hours incubation, however, all tissues were irregulary shaped in 48 hours incubation. In ammoniation, epidermal cell walls and small vascular bundles began to disintegrate by 24 hours incubation, extensively changed structure and degraded epidermal tissue by 48 hours incubation. Combination treatment of leaf sheaths degraded all cell walls of parenchyma, phloem and vascular bundle by 24 hours incubation, however, structures only of inner bundles sheath with extended land, sclerenchyma and cutinized epidermal cell walls remained.

• Korean Journal of Pharmacognosy
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• v.40 no.4
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• pp.280-285
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• 2009

Korean folk medicine 'JoRitDae' has been used mainly to cure vomiting and cancer. With regard to the botanical origin of 'JoRitDae', it has been considered to designate the rhizome of Sasa species (Gramineae), but there was no pharmacognostical confirmation on it. To clarify the botanical origin of 'JoRitDae', the anatomical characteristics of the rhizome of Sasa species growing wild in Korea and Japan, Sasa borealis, S, kurilensis, S. quelpaertensis, S. charatacea and S. senanensis were studied. As a result, it was found the morphological criteria for the five Sasa species that could discriminate them by the number of cell layers in cortex, the ratio of thickness of cortex to rhizome radius, the number of cell layers in the vascular bundle sheath, the total number of vascular bundle and thickness of vascular bundle sheath. According to these criteria, it was clarified that the commercial folk medicine 'JoRitDae' was the rhizome of Sasa borealis.

• Korean Journal of Oriental Medicine
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• v.12 no.3 s.18
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• pp.91-100
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• 2006

The purpose of this study was to introduce the differential standard of the types of Acori Graminei Rhizoma. We established the classificatory standard according to the external and internal morphology for the several types of Acori Graminei Rhizoma. 1. External shape: Acorus gramineus has smaller size and fibroidal section. A. calamus has bigger size and cancellate section. A. tatarinowii has middle size and fibroidal section. Anemone altaica has very small long-spindle shape and flat section that has 6-12 row of vascular bundle. 2. Internal shape: Acorus gramineus has some bundle sheath fiber, but A. calamus has not. A. tatarinowii has many calcium oxalate crystalloid around bundle sheath. Anemone altaica has obscure cambium. All sample except A. altaica, in the mass, has scattered bundle, thin cell wall, broad cortex and well-developed endodermis. For the future, such results will be used as the basic source of additional research, and a far-reaching comparative study is needed to distinguish between many kinds of same genus-degree of relatedness.

• Applied Microscopy
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• v.31 no.4
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• pp.367-374
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• 2001

Leaves of two developmental stages of Salsola species, young and mature, were examined to reveal the structural and functional relationships in the photosynthetic tissue using anatomical and ultrastructural criteria. Both young and mature leaves had Kranz anatomy of the Salsolid type with two layers of chlorenchyma on the leaf periphery: an outer layer of palisade mesophyll cells and an inner layer of compact bundle sheath cells with centripetally arranged organelles. The chlorenchyma was continuous in young leaves , while it was discontinuous in mature leaves. The main vascular bundle occupied the central position in the leaf. but the small peripheral vascular bundles were in contact with the chlorenchyma. Structural dimorphism of chloroplasts was obvious in bundle sheath cells of mature leaves exhibiting noticeable grana reduction, whereas mesophyll cell chloroplasts had well developed grana in all cases. Plasmodesmata were less numerous and rather simple in young leaves relative to well-developed secondary plasmodesmata of the later stage. According to the current data, features of two stages of Salsola leaves corresponded to NADP-ME bio-chemical subtype on the basis of photosynthetic cell ultrastructure. Implications of developing such anatomical and ultrastructural data of Sulsola species and biochemical characteristics reported in other C-4 species have been discussed.

• KSBB Journal
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• v.9 no.3
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• pp.319-324
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• 1994

A biosensor for the measurement of malate has been constructed by the sodium-alginate immobilized bundle sheath cell tissue of corn leaf containing malate dehydrogenase (decarboxylating) (EC 1. 1. 1. 40) on the CO2 gas-sensing electrode. The proposed tissue sensor had the linear in the range of malate concentration $5.5{\times}10^{-5}M∼2.5{\times}10^{-2}M$ with a slope of 53.5 mV/decade in 0.02M Tris-HCl buffer solution at optimum pH 8.0, and $25^{\circ}C$. A response time was 16∼18min. The present L-malate sensing tissue sensor is stable for more than one week. At pH 7.4, Km value was $0.6{\times}10^{-5}M$. The various kinds of salt did not effect the signal of malate tissue biosensor as the inhibitor. We can measure the malate by the CO2 electrode at the pH=8.0. Thus, the proposed tissue sensor will be useful for the measurement of malate.

• Journal of the Korean Wood Science and Technology
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• v.15 no.3
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• pp.14-23
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• 1987

This study was carried out to investigate the anatomical characteristics, the radial and axial variation of the cell dimension and bulk density for four bamboo species used as the major material of bamboo products in Korea; Phyllostachys(P.bambusoides, P.edulis, P.nigra var.henonis, and Pnigra). The results obtained were summarized as follows; 1) In the outer part of culm, the mophological difference of cell diameter and distribution were not acknowledged. However, gum-like substance was occurred In the epidermis, hypodermis, cortex and metaxylem vessel of P.nigra, while it was occured only in the metaxylem vessel of P.edulis. 2) The protoxylem vessel in the central zone of culm-wall could be in the two types; the first type consisted of 4-5 small cell pieces occurred in P.edulis and P.nigra var. henonis, the second type of 1-2 large cell pieces in P.bambusoides and P.nigra. And transition of cell diameter from the vascular bundle sheath to the ground tissue was gradually enlarged in P.nigra and P.edulis but abruptly in P.bambusoides and P.nigra var. henonis. 3) Thin-wall tyloses were well developed in the outer and central zone of culm-wall in four species. 4) Compared with the central part of culm-wall, the number of parenchyma cells and the amount of the vascular bundle sheaths per unit area were a few in the inner part. The inner part nearest to the pith cavity was consisted of thick-well parenchyma cell. 5) The dimensional variations of metaxylem vessel and parenchyma cell in the radial direction were decreased from the inner part to the epidermis in all species observed. 6) The fiber length was the maximum in the central zone and its diameter was increased from the inner part to the outer part. In the axial variation. fiber length was slightly increased from the base and then decresed slowly toward the top, and its diameter was reverse. 7) The axial variation of the bulk density was continuously increased from the base toward the top and its radial variation was rapidly increased from the inner part to the epidermis.

• Journal of the Korean Wood Science and Technology
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• v.29 no.2
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• pp.84-90
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• 2001

To investigate morphological characteristics of zephyr produced from two bamboo species, Phyllostachys nigra var. henonis and Gigantochloa apus, basic anatomic properties were examined by scanning electron microscopy and image analysis. According to SEM observation, zephyr from Phyllostachys nigra var. henonis was not of uniform in shape and showed macro crack between vascular bundle sheaths. This may be attributes to the sclerenchymatous fibers connected closely, thus resulting in difficult separation of intercellular layer. Zephyr from Gigantochloa apus was of uniform in shape, which may be caused by easy separation of intercellular layer of sclerenchymatous fibers having thin cell wall and large cell lumen. By image analysis in cross section of two species, the ratio of vascular bundle sheaths and cell wall ratio of sclerenchymatous fibers were examined. The ratio of vascular bundle sheaths in Phyllostachys nigra var. henonis was lower than that in Gigantochloa apus. However, cell wall ratio of sclerenchymatous fibers in Phyllostachys nigra var. henonis was higher than that in Gigantochloa apus.

• Korean journal of applied entomology
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• v.32 no.2
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• pp.208-217
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• 1993

Feeding behavior fo female brown planthoppers, Nilaparvata lugens Stal, was examined with an electrical recording technique using DC amplifier and through anatomical observation of stylet ovement in the rice plat with electrical recordings. There was six feeding patterns, type P, S, SB, O, X and Ph with the brown planthopper. Type P was a probing pattern during searching the proper feeding site. Type S appeared to be associated with the initial penetration and changing direction through the tissues, and from this type type SB pattern could be distinguished by the regularity of the large potential drops seen, and might be associated with penetration of the phloem sheath and/or salivation in the phloem sheath. The type O pattern shows none of the large voltage drops which were believed to occur when cell walls were being broken down and passes through a relatively thin layer of cells into an air space. The very constant waveform of the type X pattern could be seen during ingestion within the xylem bundle sheath area. The Ph pattern always followed an SB pattern and was associated with a marked negative voltage drop. When this pattern was seen, the brown planthopper might be ingested plant sap from phloem sheath area.

• Korean Journal of Environment and Ecology
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• v.23 no.5
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• pp.431-438
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• 2009

The main purpose of this research is to prepare and provide basic materials for the propagational strategy of eelgrass by investigating on the morphological adaptation of Korean Zostera marina to ocean currents. An eelgrass plant mainly consists of rhizome, leaf sheath, leaves and roots. The rhizome is the horizontal stem of the plant that serves as the backbone from which the leaves and roots emerge. The leaf sheath is the bundle at the base of the leaves that holds the leaves together, protecting the meristem, the primary growth point of the shoot. Leaves originate from a meristem which is protected by a sheath at the actively growing end of the rhizome. As the shoot grows, the rhizome elongates, moving across or within the sediment, forming roots as it progresses. The aggregated leaves from the leaf sheath are found to have two cell layers on one side and multiple layers of airy tissues called aerenchyma on the other. The aerenchyma tissues are developed in multi-layered cell structures surrounding the veins which are formed in the leaf sheath. Generative shoots are made of rhizomes, which are circular or ovoidal, stem, and spathe and spadix. The transverse section of rhizome and the stem and central floral axis is found to be circular, ovoid and in the shape of convex respectively, and the vascular bundle, which is a part of transport system, has one large tube in the center and two small tubes on both sides. The layers of collenchyma cells numbered from 12 to 15 in the stem, and from 7 to 12 in the rhizome. The seed coat is composed of sclereids, small bundles of sclerenchyma tissues, which prevent the influx of sea water from the outside and help endure the environmental stress. In conclusion, alternative multi-layer structure in circular, convex type aggregated leaf base are interpreted to morphological adaption as doing tolerable elastic structure through movement of seawater. The generative shoots develop long slim stem and branches in circular or ovoidal shapes to minimize the adverse impacts of sea current, which can be interpreted as the plant's morphological adaptation to its environment.