• Preventive Nutrition and Food Science
• /
• 제3권2호
• /
• pp.143-151
• /
• 1998

Protein -surfactant interactions have been investigate by measuring ζ-potential of $\beta$-lactoglobulin-coated emulsion droplets and $\beta$-lactoglobulin in solution in the rpesenceof surfactant, with particular emphasis on the effect of protein heat treatment(7$0^{\circ}C$, 30min). When ionic surfactant (SDS or DATEM) is added to the protein solution, the ζ-potential of the mixture is found to increase with increasing surfactant concentration, indicating surfactant binding to the protein molecules. For heat-denatured protein,it has been observed that the ζ-potential tends to be lower than that of the native protein. The effect of surfactant on emulsions is rather complicated .With SDS, small amounts of surfactant addition induce a sharp increase in zeta potential arising from the specific interaction of surfactant with protein. With further surfacant addition, there is a gradual reductio in the ζ-potential, presumably caused by the displacement of adsorped protein (and protein-surfactant complex) from the emulsion droplet surfac by the excess of SDS molecules. At even higher surfactant concentrations, the measured zeta potential appears to increase slightly, possibly due to the formation of a surfactant measured zeta potential appears to increase slightly, possibly due to the formation of surfactant micellar structure at the oil droplet surface. This behaviour contrastswith the results of the corresponding systems containing the anionic emulsifier DATEM, in which the ζ-potential of the system is found to increase continuously with R, particularly at very low surfactant concentration. Overall, such behaviour is consisten with a combination of complexation and competitive displacement between surfactant and protein occurring at the oil-water interface. In addition, it has also been found that above the CMC, there is a time-dependent increase in the negative ζ-potential of emulsion droplets in solutions of SDS, possibly due to the solublization of oil droplets into surfactant micelles in the aqueous bulk phase.

• 폴리머
• /
• 제33권1호
• /
• pp.7-12
• /
• 2009

PLGA미립구는 주사제형과 같은 형태로 조직공학적 응용에 이용할 수 있다. 본 연구에서는 미립구 제조시 에밀젼 형성조건에 대한 영향과 미립구 표면에 세포를 부착시키는 방법에 대하여 연구하였다. BSA를 함유하는 PLGA미립구는 수중유형(O/W)과 수중유중수형(W/O/W) 용매증발법을 이용하여 제조하였다. 미립구의 초기방출효과제어와 PLGA분해의 과정에서 발생되는 지연시간을 개선시키기 위하며 알긴산나트륨을 수상에 용해시켜 사용하였다. 미립구에 부착된 세포의 형태를 전자주사현미경(SEM)을 이용하여 분석하였고 PLGA미립구에 배양된 인간디스크세포의 증식은 MTT분석을 이용하였으며 이를 통하여 PLGA미립구 표면에 세포가 부착되었음을 확인하였다. 본 연구는 BSA가 함유된 알긴산/PLGA미립구를 이용하여 조직공학적 응용이 가능한 주사제형으로서의 가능성을 제안하였다.