• Title/Summary/Keyword: Buff

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Identification and Pathogenicity of Binucleate Rhizoctonia Isolates Causing Leaf Blight(Yellow Patch) in Turfgrass (잔디의 잎마름증상(Yellow patch)을 일으키는 2핵성 Rhizoctonia의 동정 및 병원성)

  • 김진원;심규열;김호준;이두형
    • Asian Journal of Turfgrass Science
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    • v.6 no.2
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    • pp.99-111
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    • 1992
  • Yellow patch as leaf blight caused by binucleate Rhizoctonia occured in bentgrass (Agrostis Palustris Huds), zoysiagrass (Zoysia japonica Steud) and Kentucky bluegrass (Poa pratensis L.) from several golf couses in Korea. Binucleate Rhizoctonia was isolated from the infected lesions and was identified Rhizoctonia cerealis. Rhizoctonia cerealis infected crown, stem and leaf tissue, and the symptom was light yellow circular patch upto 1 m in diameter on bentgrass golf green. Individual infected leaf near the margin of patch developed first red and finally turn brown. As zoysiagrass lawn, the symptom was 30~40cm circular patch that occured zoysiagrass shooting time as spring, and there could not sheeted in severe lesion. In case of sheeted, zoysiagrass was first irregular leaf sopt and finally dead. Hypha diameter of Rhizoctonia cerealis was $2.5~6.3\mu\textrm{m}$(average $3.8\mu\textrm{m}$) and colar was white to buff. Monilioid cell size was $5.8~12.5$\times$13.8~37.5\mu\textrm{m}$. Sclerotia size was 0.2~2.0mm and color was white to brown. Optium temperature for the hypha growth was $23^{\circ}C$. There was a little difference in pathogenicity among the isolates.

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Colonial Morphology of Yeasts Grown on the Medium Containing Pigments Extracted from the Fruits of Gardenia jasminoides (수용성 치자(水溶性 梔子)(Gardenia jasminoides열매)색소(色素)를 첨가(添加)한 배지(培地)에 배양(培養)한 각종(各種) 효모균류(酵母菌類) 균집락(菌集落)의 형태(形態))

  • Kim, Sang-Jae;Kim, Sin-Ok;Jeong, Suk
    • The Korean Journal of Mycology
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    • v.19 no.2
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    • pp.136-142
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    • 1991
  • Colonial morphology of the various yeasts grown on the yeast morphology agar me­dium containing orange-yellow pigments extracted from the fruits of Gardenia jasminoides (GJPM) was investigated in hopes of the differential identification of yeasts on primary cultures. Colonies of Candida lusitaniae and Ca. guilliermondii on GJPM turned to prussian blue within three days of incubation and Ca. tropicalis and Ca. viswanathii turned to bluish gray but the latter species turned to deep blue after 7 days. Ca. krusei, Saccharomyces cerevisiae, and Torulopsis glabrata showed neutral gray, grayish green, and baby blue respectively after one or two weeks. However, the colonies of Ca. albicans and parapsilosis remained unchanged even after 20 days. Colonial color of Cryptococ­cus neoformans showing brown to purple brown was distinguishable not only from buff color of Cr. laurentii after one or two weeks incubation but also from those of Candida spp. Growth of certain species was promoted on GJPM. The findings clearly showed that Ga. jasminoides pigments medium was useful to the morphological differentiation of medically important yeasts which were often encountered in sputum or other clinical specimens.

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Study on brewing of sweet potato starch (고구마 전분질원료를 이용한 주류제조에 관한 연구)

  • 정기택;유대식
    • Korean Journal of Microbiology
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    • v.9 no.3
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    • pp.103-120
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    • 1971
  • We have been studied on brewing sweet starch. We obtained the results as follows ; 1) 5 strains, T-T-2, T-T-4, T-K-2, T-T-18, T-T-1, were the most available in view of fermentative power by capacity of $CO_2$. 2) 5 strains, T-T-4, T-T-2, T-T-1, T-T-3, T-K-2, produced capacity of alcohol more than 5.78%. 3) 6 strains, T-T-2, T-K-2, T-T-4, T-S-2, T-I-3, T-I-1, are available not only taste and flavour, but productive power of alcohol in sweet potato starch. 4) The form of 6 strains are long oval and round and most of them are similar to the other yeast in size. 5) In giant colony the color was cream color and cream buff, and T-K-2 was formed by $15{\times}12mm$ on diameter and by 3.5mm on high. 6) Optimum temperature of most of all strains is 25~ $300^{\circ}C$but T-K-4 is 28-30.deg.C. 7) Optimum pH is 3.4-4.6. 8) T-S-2 was died off at 65.deg.C, the other strains died $60^{\circ}C$. 9( Making Bun-kok with non-heated wheat bran .alpha.-amylase was more increased by 4.5-13.5 mg of glucose in reaction solution and .betha.-amylase more 1.6-3.4ml of N/10-$KMnO_4$ Solution than Bun-kok with heated wheat bran. 10) It seems that mycellium grows better than original in substance containing 0.4 ~ 1.2% of HCl. 11) Making Bun-kok to add 0.8% HCl, .alpha.-amylase was increased 9.93-20.7mg of glucose and .betha.-amylase ws increased 2.6~4.3ml of N/10-$KMnO_4$ solution to reaction solution. 12) 1.2%-HCl, or higher concentration, acts as inhibitor, in the meanwhile the concentration between 0.4~0.8% of HCl acts as activator. 13) We must make Bun-kok for 42 hours, at 28~$30^{\circ}C$) After we made Bun-kok using S-O-II and R-J-I one by one, Bun-kok which mix each other in equal quantity is increased more than original on enzyme acrivity. 15) Oxidation is the best way of refining sweet potato starch in N/10-phosphate buffer solution (pH 7.5). 16) When we prepared sweet potato starch, first pH was 3.0.

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Breeding of Lilium Asiatic 'Lovely LiASong' as an F1 Hybrid for Pot (일대잡종 분화용 아시아틱나리 'Lovely LiASong' 육성)

  • Song, C.Y.;Park, B.M.;Moon, J.Y.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.20 no.2
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    • pp.5-11
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    • 2018
  • There is a need to breed and distribute domestic varieties for Lilium Asiatic hybrids because most of the lily bulbs cultivated in Korea imported from foreign country. The genetic resources of Lilium Asiatic hybrids, 'Buff Pixie', 'Orange Matrix' and 'Tiny Puppet', which were collected from 2001, had been self-pollinated from 2004 to 2012. In 2012, Lilium Asiatic 'Lovely LiASong' as an F1 hybrid was bred through crossing 'L2-12-1' and 'L2-35-4' obtained by 4th self-fertilization. 'Lovely LiASong' had a bowl shaped single flower with a few spots and medium-sized flower, 8.7cm in flower diameter. The number of flowers per plant was 4.9, which was relatively more flowers than other cultivars. And the flower inflorescence had umbellate and racemose while the attitude of longitudinal axis was erect. The plant height was 33.4cm with 30.4 leaves per plant and 7.2cm in leaf length. The 'Lovely LiASong' showed a high consumer preference because it had a relatively longer flowering period, larger flower diameter and number of flowers than those of the control, 'Tiny Ghost'.