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In-vitro Antithrombosis Activity of Different Parts of Sorbus commixta from Ulleung Island (울릉도 마가목의 다양한 부위의 항혈전 활성)

  • Kim, Mi-Sun;Seong, Ha-Jung;Sohn, Ho-Yong
    • Journal of Life Science
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    • v.26 no.3
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    • pp.289-295
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    • 2016
  • Sorbus commixta, a flowering plant in the Rosaceae family, is native to Japan and Ulleung Island, Korea. This plant is also called maga-mok or mai-mok in Korea because the bud of the stem has a similar shape to the teeth of a horse. In this study, hot water extracts from different parts of S. commixta, such as leaf, stem, and immature and mature fruits, were prepared, and their antithrombosis and antioxidant activities were evaluated. The extraction yield and pH of stem extracts were 3.99% and 5.5, respectively. The stem extracts contained 89.2 mg/g of total polyphenols and 28.3 mg/g of total flavonoids. The hot water extracts prepared from the leaf, stem, immature, and mature fruit of S. commixta exhibited no hemolytic activity against human red blood cells, up to a concentration of 0.5 mg/ml. In an anticoagulation assay, the stem extracts showed strong extension in thrombin, prothrombin, and activated partial thromboplastin times, whereas the other extracts had no anticoagulation activity. In a platelet aggregation inhibitory activity assay, all the extracts tested had no inhibitory activity against human platelets. With regard to antioxidation activity, the stem extracts showed stronger radical scavenging activity and reducing power activity than the other extracts. The calculated RC50s, the concentration required for 50% radical scavenging activity, for DPPH anions, ABTS cations, and nitrite of the crude stem extracts were 119.7, 53.3, and 117.5 μg/ml, respectively, whereas they were 13.7, 5.2, and 14.9 μg/ml for DPPH anions, ABTS cations, and nitrite, respectively, for vitamin C. The results suggest that the stem extracts of S. commixta have strong potential for use as a novel resource for antithrombosis agents.

Pink Rot of Palms Caused by Gliocladium vermoseni (Gliocladium vermoeseni에 의한 야자 분홍썩음병(가칭))

  • Han, Kyung-Sook;Park, Jong-Han;Lee, Jung-Sup;Seo, Sang-Tae;Jang, Han-Ik
    • Research in Plant Disease
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    • v.10 no.4
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    • pp.345-348
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    • 2004
  • Pink rot of Palm was occurred at Yeuju area in 2001 and 2003. Infected plants showed rotting at the leaf-stock bases and killing of the terminal bud. The first symptoms are dark brown necrotic areas on the stem. Bases of infected frond may be covered with pink spores and the spots produced oozing gum pockets. Oozing lesions occur on the stems, and leaves turn brown and droop. The causal agent were isolated from salmon-pink spores sporulating on the leaf sheaths and necrotic stem tissues. Pathogen were isolated from freshly infected tissues were identified as Gliocladium vermoseni based on mycological characteristics. Fungus were grown plenty on PDA culture. Temperature for mycelial grown was tested at 5 to $40^{\circ}C$ and optimal temperature was $25^{\circ}C$ and was not nearly grew at temperature above $35^{\circ}C$. Artificial pathogenicity were tested on 9 species of Palm family in the wound inoculation and symptoms showed similar to those observed in the field. This is the first report on pink rot of palm in Korea.

Morphological study on the tongue of Korean native goat (한국재래산양 혀에 관한 형태학적 연구)

  • Lee, Heungshik S.;Lee, In-se;Kang, Tae-cheon
    • Korean Journal of Veterinary Research
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    • v.36 no.2
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    • pp.255-264
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    • 1996
  • This studies were carried out to identify the characteristics of the tongue of Korean native goat(Capra hircus) by macroscopy, microscopy and scanning microscopy. Korean native goat had torus linguae, median lingual sulcus, lingual fossa and ventral median fissure but did not have glossoepiglottic fold and terminal sulcus in the tongue. The whole length of tongue was $11.51{\pm}0.76cm$. The length of tongue apex, tongue body, tongue root and the torus linguae were $2.62{\pm}0.28$, $7.39{\pm}0.27$, $1.56{\pm}0.26$ and $6.37{\pm}0.29cm$, respectively. The width of tongue apex, torus linguae and tongue root were $3.41{\pm}0.24$, $3.74{\pm}0.29$ and $3.68{\pm}0.11$, respectively. The thickness of tongue apex was $1.60{\pm}0.10$, and the height of torus linguae was $1.52{\pm}0.15cm$. Filiform papillae were present at the tongue apex and the tongue body rostral to torus linguae. Fungiform papillae were scattered from tongue apex to rostral portion of torus linguae, being in abundance at the tongue apex. Vallate papillae were showed at the lateral portion of torus linguae, while lentiform papillae were present at its central portion. Conical papillae were located between vallate and lentiform papillae. The numbers of filiform, fungiform, conical, vallate and lentiform papillae were $46,980{\pm}1070.98$, $446.8{\pm}36.97$, $818.4{\pm}43.99$, $34.8{\pm}2.77$, and $255.6{\pm}39.30$, respectively. The average numbers of taste bud were $8.3{\pm}2.04$ in a fungiform papilla and $247.3{\pm}37.44$ in a vallate papilla. The filiform papilla had secondary and tertiary papillae. The height of filiform papilla was about $150{\mu}m$ and the diameter was $100{\mu}m$. The diameters of fungiform papillae were 350 to $550{\mu}m$. The long and short diameters of maximum-sized lentiform papilla were 4000 and $3000{\mu}m$, respectively, while those of minimum-sized papilla were 700 and $600{\mu}m$, respectively. The height of conical papillae was 450 to $600{\mu}m$ and diameter was 250 to $450{\mu}m$. The vallate papilla was round or oval in shape and its diameter was 500 to $850{\mu}m$. It had well-developed papillary groove around itself. The modified conical papillae were not observed in the tongue of Korean native goat.

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Studies on the Cause and Control for Non-Sprouting in the Mulberry Field of Kyung-pook Province (경북지방의 뽕밭에 발생한 발아불량현상의 원인 및 방제에 관한 연구)

  • Yu, Geun-Seop;Kim, Gyu-Rae;Kim, Nak-Sang
    • Journal of Sericultural and Entomological Science
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    • v.28 no.2
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    • pp.1-8
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    • 1986
  • This study was conducted to bring light on inducing factors for non-sprouting occured in the mulberry field of Kyungpook Province in 1983. The results are as follws ; 1. In spring, winter buds were suddenly died during germination, necrosis appeared in pholem and cortex of non-sprouting stem and measles at the bottom of non-sprouting stem. 2. The depth of available of soil was more shallow in the non-sproutiong field than in healthy field. 3. There was no signifioant difference between the healthy field and non-sprouting field in siol PH, the content of organic matter, available phosphorous and exchangeble cations. 4. Available boron content in soil was significantly more in healthy field than in non-sprouting field. 5. Boron content in leaf and bark was significantly lower in the non-sproutiong tree than in the healthy tree. 6. The non-sprouting fields were completely controlled by the application of 6kg/10a borax. 7. Cold tolerance of the mulberry tree was higher in the mulberry tree with boron than in the mulberry tree without boron. 8. Mulberry tree in Kyungpook Province in 1983 may absorb water earlier compared with the average year because of high temperature at the end of March, 1983 and they had been damaged by frost injuary due to the lowest temperature (-6.4$^{\circ}C$) on the grass at the beginning of April 1983. As the above results, non-sprouting bud in the mulberry fields of Kyungpook Province in 1983 had been occured by low temperature and boron deficiency and boron deficiency of mulberry trees accelerared the frost injury.

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Evaluation of Embryotoxic Potential of Olaquindox and Vitamin A in Micromass Culture and in Rats

  • Kang, Hwan-Goo;Ku, Hyun-Ok;Jeong, Sang-Hee;Cho, Joon-Hyoung;Son, Seong-Wan
    • Toxicological Research
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    • v.26 no.3
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    • pp.209-216
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    • 2010
  • Limb bud (LB) and central nerve system (CNS) cells were prepared from 12.5 day old pregnant female Crj:CD (SD) rats and treated with olaquindox and vitamin A. Cytotoxicity and inhibition on differentiation were measured in each cell. Three doses of olaquindox (4, 21 and 100 mgkg), and 0.2 and 75 mg/kg of vitamin A were administered to pregnant rat for 11 days from $6^{th}$ to $16^{th}$ of pregnancy. $IC_{50}$ values of olaquindox for proliferation and differentiation in CNS cell were 22.74 and $28.32\;{\mu}g/ml$ and 79.34 and $23.29\;{\mu}g/ml$ in LB cell and those values of vitamin A were 8.13 and $5.94\;{\mu}g/ml$ in CNS cell and 0.81 and $0.05\;{\mu}g/ml$ in LB cell, respectively. Mean body weights of pregnant rats were decreased at high dose of olaquindox (110 mg/kg) but relative ovary weight, number of corpus lutea, and number of implantation were not changed. Resorption and dead fetus were increased at high dose of olaquindox, and relative ovary weight, the number of corpus lutea and implantation, and sex ratio of male to female were not significantly changed in all dose of olaquindox. Mean fetal and placenta weights were significantly (p < 0.01) decreased in rats of high group. Seven fetuses out of 103 showed external anomaly like bent tail, and 10 out of 114 fetuses showed visceral anomalies at high group. The ossification of sternebrae and metacarpals were significantly (p < 0.01) increased by low and middle dose of olaquindox but it was significantly (p < 0.01) prohibited by high dose of olaquindox. In rats treated with vitamin A, the resorption and dead fetus were increased by high dose. Mean fetal weights were significantly (p < 0.01) increased by low dose but significantly (p < 0.01) decreased by high dose. Thirty four fetuses out of 52 showed external anomaly; bent tail (1), cranioarchschisis (14), exencephaly (14), dome shaped head (22), anophthalmia (15), brcahynathia (10) and others (19). Forty five fetuses out of 52 showed soft tissue anomaly; cleft palate (42/52) and anophthalmia (22/52) by high dose of vitamin A. Sixty one fetuses out of 61 (85.2%) showed skull anomaly; defect of frontal, partial and occipital bone (21/61), defect of palatine bone (52/61) and others (50/61). In summary, we support that vitamin A is strong teratogen based on our micromass and in vivo data, and olaquindox has a weak teratogenic potential in LB cell but not in CNS cell. We provide the in vivo evidence that a high dose of olaquindox could have weak embryotoxic potential in rats.

Modeling for Predicting Yield and $\alpha$-Acid Content in Hop (Humulus lupulus L.) from Meteorological Elements I. A Model for Predicting Fresh Cone Yield (기상요소에 따른 호프 (Humulus lupulus L.)의 수량 및 $\alpha$-Acid 함량 예측모형에 관한 연구 I. 생구화 수량 예측모형)

  • 박경열
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.3
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    • pp.215-221
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    • 1988
  • The hop yield prediction model developed based on meteorological elements in Hoeongseong was Y=6,042.846-17.665 $X_1$-0.919 $X_2$-96.538 $X_3$-138.105 $X_4$+86.910 $X_{5}$$X_{6}$ with MS $E_{p}$ of 25.258, $R_{p}$$^{2}$ of 0.9991, R $a_{p}$$^{2}$ of 0.9962 and $C_{p}$ of 7.00. The minimum air temperature at early growing stage ( $X_1$), the total precipitation at cone ripening stage ( $X_2$), the maximum air temperature at flower bud differentiation stage ( $X_3$) and the maximum air temperature at flowering stage ( $X_4$) influenced on hop yield as decrement weather elements. The average air temperature at early growing stage ( $X_{5}$ ) and the total sunshine hours at cone development stage ( $X_{6}$ ) influenced on hop yield as increment weather elements.lements.

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Studies on Mechanism and Damage Occurrence for Foliar Spray of NaCl Solutions in Fruit Trees (NaCl 엽면살포에 따른 과수의 피해양상과 기작에 관한 연구)

  • Kim, Seung-Heui;Song, Gi-Cheol;Park, Jeong-Gwan;Park, Moo-Yong
    • Korean Journal of Environmental Agriculture
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    • v.25 no.4
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    • pp.352-358
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    • 2006
  • We investigated the mechanism on occurring of briny injury in four fruit species (apple, pear, grape, peach). Briny injury of apple, pear, peach, and grape was induced by the foliar splay of saline solutions. Browning symptom was initiated one hour after the spray of 3% NaCl in apple, pearl and peach trees. while two hour were needed for grape, but the latter developed the symptom faster than the formers. Foliar spray of 3% NaCl resulted in 4-fold increase in ethylene production 24 h after the spray, and the production was returned to early stage rate after 72 h. Ethylene production of control was nearly constant with slight decrease after 4 h. Browning and defoliation response to the spray of 3% NaCl on lower side were not different from the spray on both sides regardless of fruit species, while no injury symptoms occurred by the spiny on upper side. In saline damage for treated date, lateral bud was only germinated in apple at 30 July. A shoot apex was 100% grew in all fruit tree. In result for hormonal content of leaf and shoot apex, control was high t-zeatin content in leaf. However, IAA and ABA content was increasing in high saline concentration.

Determination of Glucose Distribution of Potato Tuber Using Blood Glucose Meter and Its Application to Estimate Processing Quality (혈당측정기를 이용한 감자 괴경의 포도당 분포 분석과 이의 가공적성 평가에의 활용)

  • Kwon, Byoung-Hoon;Kim, Si Un;Lim, Soo Yeon;Namgung, Hyeju;Lee, Hong Jin
    • Horticultural Science & Technology
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    • v.32 no.2
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    • pp.171-177
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    • 2014
  • In an effort to investigate special distribution of glucose content of potato tuber of a cultivar 'Superior' after harvest, a whole tuber was longitudinally cut into halves and cut-surface was divided into 11 regions. Approximate glucose concentration of each section was determined using a commercial blood glucose meter. Higher level of glucose was detected in the outer layers of tissue than inner part of tuber although there were lower coefficients of variation value, 37.4% and 34.1% among individual tubers and among defined sections, respectively. A positive correlation between the whole tuber and individual section was existed in glucose content, where the central pith tissue gave the highest coefficient (r = 0.921) and bud end tissue did the lowest (r = 0.544). Glucose content of the tubers stored for 4 months at $2.0^{\circ}C$ was 5.5 fold higher compared to the tubers kept in ambient temperature. The chip color of the former tubers was much brighter than those of the latter tubers. The result obtained in the present study suggests that the blood glucose meter can be used to a rapid and simple evaluation of glucose content and therein be applied to estimate the processing quality of potato tubers during postharvest handling.

Feasibility of Stochastic Weather Data as an Input to Plant Phenology Models (식물계절모형 입력자료로서 확률추정 기상자료의 이용 가능성)

  • Kim, Dae-Jun;Chung, U-Ran;Yun, Jin-I.
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.14 no.1
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    • pp.11-18
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    • 2012
  • Daily temperature data produced by harmonic analysis of monthly climate summary have been used as an input to plant phenology model. This study was carried out to evaluate the performance of the harmonic based daily temperature data in prediction of major phenological developments and to apply the results in improving decision support for agricultural production in relation to the climate change scenarios. Daily maximum and minimum temperature data for a climatological normal year (Jan. 1 to Dec. 31, 1971-2000) were produced by harmonic analysis of the monthly climate means for Seoul weather station. The data were used as inputs to a thermal time - based phenology model to predict dormancy, budburst, and flowering of Japanese cherry in Seoul. Daily temperature measurements at Seoul station from 1971 to 2000 were used to run the same model and the results were compared with the harmonic data case. Leaving no information on annual variation aside, the harmonic based simulation showed 25 days earlier release from endodormancy, 57 days longer period for maximum cold tolerance, delayed budburst and flowering by 14 and 13 days, respectively, compared with the simulation based on the observed data. As an alternative to the harmonic data, 30 years daily temperature data were generated by a stochastic process (SIMMETEO + WGEN) using climatic summary of Seoul station for 1971-2000. When these data were used to simulate major phenology of Japanese cherry for 30 years, deviations from the results using observed data were much less than the harmonic data case: 6 days earlier dormancy release, 10 days reduction in maximum cold tolerance period, only 3 and 2 days delay in budburst and flowering, respectively. Inter-annual variation in phenological developments was also in accordance with the observed data. If stochastically generated temperature data could be used in agroclimatic mapping and zoning, more reliable and practical aids will be available to climate change adaptation policy or decision makers.

Enfluence of the Number of the Lower Scaffold Limbs in Slender Spindle Form on the Tree Growth and Development of 'Fuji' Apple Trees ('후지' 사과나무 세장방추형에서 하단측지수가 수체생육에 미치는 영향)

  • Park, Moo-Yong;Yang, Sang-Jin;Park, Jeung-Kwan;Choi, Dong-Geun;Kang, In-Kyu
    • Journal of Bio-Environment Control
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    • v.16 no.3
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    • pp.258-263
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    • 2007
  • This study was carried out to investigate the effects of number of the lower scaffold limbs on tree growth, light penetration, fruit yield, and fruit quality in slender spindle in 6-year-old 'Fuji'/M.9 apple trees. With regard to the growth by the numbers of the lower scaffold limbs, the width of the tree was wide and the growth of new shoots was increased when the number of the lower scaffold limbs was five. Compare with other treatments, five lower scaffold limbs showed high light-interception on the upper (150 cm above the ground) and middle (100 cm above the ground) canopy. There was no difference in the total number of the flower buds of the spurs according to the number of scaffold limbs, but the number and cross section area of flower bud on the lower canopy (120 cm above the ground) were increased where the number of the lower scaffold limbs was five. Fruit yield was highest in the treated with five lower scaffold limbs and fruit weight tended to increase where the number of the lower scaffold limbs was five or eight. With regard to fruit quality, there showed no difference in fruit shape index, firmness, acid content, Hunter L and b value according to the location of canopy and the number of the lower scaffold limbs, but the content of soluble solids was highest treated with five lower scaffold limbs. Hunter a value indicating fruit color was found to be highest treated with five lower scaffold limbs whose light interception was highest.