• 구강회복응용과학지
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• 제36권2호
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• pp.88-94
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• 2020

목적: 본 연구는 한국인에서 상악 전치의 치근 위치를 시상면에서 분석하고, 분류에 따른 빈도를 보고함으로써 즉시 식립 임플란트를 위한 방사선학적 자료를 수집하기 위함이다. 연구 재료 및 방법: 콘빔형 전산화단층영상(cone-beam CT)을 촬영한 환자 중 연구 기준에 적합한 120명(남성 60명, 여성 60명)을 대상으로 후향적 분석을 시행하였다. 축의 방향설정을 시행한 후에, 상악 전치부 치아와 치조골 사이의 관계에 대한 시상 치근 위치를 분석하였다. 치근이 치조골의 협측, 중앙, 구개측으로 위치한 경우 각각 Class I, II, III로 분류하였으며, 치근이 협측과 구개측 모두에서 피질골판에 2/3 이상 닿아있는 경우에는 Class IV로 하였다. 다음으로, 치아의 장축과 치조골의 장축 사이의 각도를 측정하였다. 기술적 분석 및 Kruskal-Wallis 분석을 시행하였으며, 치아의 위치 및 분류에 따른 시상각을 비교하였다. 결과: 상악 전치부의 시상 치근 위치에 대한 빈도분석 결과, Class I은 81.1%, Class II는 10.3%, Class III는 1.9%, 그리고 Class IV는 6.7%로 나타났다. 상악 전치부의 77.5%에서 시상각이 20도 이하로 나타났다. 그러나 견치의 경우, 42.7%에서 20도 이상의 시상각을 보였다. 분류에 따라서는 Class I (16.19)에서 Class II (8.72) 및 Class III (9.93)에 비해 통계학적으로 유의하게 높은 시상각을 보였으며, Class IV (3.79)에서 낮았다. 결론: 본 제한된 연구의 결과를 근거로, 상악 전치의 치근은 일반적으로 협측 치조골에 가깝게 위치하고 있으나, 일부 치아는 매우 얇은 치조골을 가지고 있으며, 30도 이상의 큰 시상각을 보였다. 따라서 적절한 치과 임플란트 치료 계획 수립을 위해 시상 치근 위치 및 시상각에 대한 cone-beam CT 분석이 필요할 것이다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제32권4호
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• pp.299-305
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• 2010

Purpose: Adipose tissue is located beneath the skin, around internal organs, and in the bone marrow in humans. Its main role is to store energy in the form of fat, although it also cushions and insulates the body. Adipose tissue also has the ability to dynamically expand and shrink throughout the life of an adult. Recently, it has been shown that adipose tissue contains a population of adult multipotent mesenchymal stem cells and endothelial progenitor cells that, in cell culture conditions, have extensive proliferative capacity and are able to differentiate into several lineages, including, osteogenic, chondrogenic, endothelial cells, and myogenic lineages. Materials and Methods: This study focused on endothelial cell culture from the adipose tissue. Adipose tissues were harvested from buccal fat pad during bilateral sagittal split ramus osteotomy for surgical correction of mandibular prognathism. The tissues were treated with 0.075% type I collagenase. The samples were neutralized with DMEM/and centrifuged for 10 min at 2,400 rpm. The pellet was treated with 3 volume of RBC lysis buffer and filtered through a 100 ${\mu}m$ nylon cell strainer. The filtered cells were centrifuged for 10 min at 2,400 rpm. The cells were further cultured in the endothelial cell culture medium (EGM-2, Cambrex, Walkersville, Md., USA) supplemented with 10% fetal bovine serum, human EGF, human VEGF, human insulin-like growth factor-1, human FGF-$\beta$, heparin, ascorbic acid and hydrocortisone at a density of $1{\times}10^5$ cells/well in a 24-well plate. Low positivity of endothelial cell markers, such as CD31 and CD146, was observed during early passage of cells. Results: Increase of CD146 positivity was observed in passage 5 to 7 adipose tissue-derived cells. However, CD44, representative mesenchymal stem cell marker, was also strongly expressed. CD146 sorted adipose tissue-derived cells was cultured using immuno-magnetic beads. Magnetic labeling with 100 ${\mu}l$ microbeads per 108 cells was performed for 30 minutes at $4^{\circ}C$ a using CD146 direct cell isolation kit. Magnetic separation was carried out and a separator under a biological hood. Aliquous of CD146+ sorted cells were evaluated for purity by flow cytometry. Sorted cells were 96.04% positivity for CD146. And then tube formation was examined. These CD146 sorted adipose tissue-derived cells formed tube-like structures on Matrigel. Conclusion: These results suggest that adipose tissue-derived cells are endothelial cells. With the fabrication of the vascularized scaffold construct, novel approaches could be developed to enhance the engineered scaffold by the addition of adipose tissue-derived endothelial cells and periosteal-derived osteoblastic cells to promote bone growth.

• 대한구순구개열학회지
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• 제3권1호
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• pp.23-31
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• 2000

전방 기저 설피판은 비교적 큰 구개 결손을 폐쇄하는데 안전하고 효과적이었으며 후에 장골 이식을 위한 건전한 피복을 제공하였다. 그리고 치조열 결손의 순측면까지 완전히 피복하기 위한 목적으로 양측성 치조열에 적용되도록 재단된 Y-형의 변형은 결과가 믿을 만하였으며 합병증도 무시할 만 하였다.

• Restorative Dentistry and Endodontics
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• 제22권1호
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• pp.1-33
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• 1997

The objective of this study was to analyze the in vitro and in vivo corrosion products of low and high copper amalgams. The four different types of amalgam alloy used in this study were Fine cut, Caulk spherical, Dispersalloy, and Tytin. After each amalgam alloy and Hg were triturated according to the directions of the manufacturer by means of the mechanical amalgamator(Amalgam mixer. Shinhung Co. Korea), the triturated mass was inserted into a cylindrical metal mold which was 12mm in diameter and 10mm in height. The mass was condensed by 150Kg/cm compressive force. The specimen was removed from the mold and aged at room temperature for about seven days. The standard surface preparation was routinely carried out by emery paper polishing under running water. In vitro amalgam specimens were potentiostatically polarized ten times in a normal saline solution at $37^{\circ}C$(potentiostat : HA-301. Hukuto Denko Corp. Japan). Each specimen was subjected to anodic polarization scan within the potential range -1700mV to+400mV(SCE). After corrosion tests, anodic polarization curves and corrosion potentials were obtained. The amount of component elements dissolved from amalgams into solution was measured three times by ICP AES(Inductive Coupled Plasma Atomic Emission Spectrometry: Plasma 40. Perkim Elmer Co. U.S.A.). The four different types of amalgam were filled in occlusal and buccal class I cavities of four human 3rd molars. After about five years the restorations were carefully removed after tooth extraction to preserve the structural details including the deteriorated margins. The occlusal surface, amalgam-tooth interface and the fractured surface of in vivo amalgam corrosion products were analyzed. In vivo and in vitro amalgam specimens were examined and analyzed metallographically by SEM(Scanning Electron Microscope: JSM 840. Jeol Co. Japan) and EDAX(Energy Dispersive Micro X-ray Analyser: JSM 840. Jeol Co. Japan). 1. The following results are obtained from in vitro corrosion tests. 1) Corrosion potentials of all amalgams became more noble after ten times passing through the in vitro corrosion test compared to first time. 2) After times through the test, released Cu concentration in saline solution was almost equal but highest in Fine cut. Ag and Hg ion concentration was highest in Caulk spherical and Sn was highest in Dispersalloy. 3) Analyses of surface corrosion products in vitro reveal the following results. a)The corroded surface of Caulk spherical has Na-Sn-Cl containing clusters of $5{\mu}m$ needle-like crystals and oval shapes of Sn-Cl phase, polyhedral Sn oxide phase. b)In Fine cut, there appeared to be a large Sn containing phase, surrounded by many Cu-Sn phases of $1{\mu}m$ granular shapes. c)Dispersalloy was covered by a thick reticular layer which contained Zn-Cl phase. d)In Tytin, a very thin, corroded layer had formed with irregularly growing Sn-Cl phases that looked like a stack of plates. 2. The following results are obtained by an analysis of in vivo amalgam corrosion products. 1) Occlusal surfaces of all amalgams were covered by thick amorphous layers containing Ca-P elements which were abraded by occlusal force. 2) In tooth-amalgam interface, Ca-P containing products were examined in all amalgams but were most clearly seen in low copper amalgams. 3) Sn oxide appeared as a polyhedral shape in internal space in Caulk spherical and Fine cut. 4) Apical pyramidal shaped Sn oxide and curved plate-like Sn-Cl phases resulted in Dispersalloy. 5) In Tytin, Sn oxide and Sn hydroxide were not seen but polyhedral Ag-Hg phase crystal appeared in internal space which assumed a ${\beta}_l$ phase.