• Journal of the Korean Crystal Growth and Crystal Technology
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• v.16 no.5
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• pp.198-202
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• 2006

We could achieve the bridge-type formation of the iridium-catalyzed carbon nanofibers across the gap on the MgO substrate using microwave plasma enhanced chemical vapor deposition method. On the plane surface area of the MgO substrate, the iridium-catalyzed carbon nanofibers were grown as a lateral direction to the substrate. The bridge-type formation and/or the lateral growth of the iridium-catalyzed carbon nanofibers were interconnected with each other. Finally, they could form an entangled network having the bridge-type formation of the carbon nanofibers across the gap on the substrate and the laterally-grown carbon nanofibers on the plane surface area of the substrate. The entangled network showed the semiconductor electrical characteristics.

• Smart Structures and Systems
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• v.5 no.4
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• pp.381-395
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• 2009

The paper presents a case study in which the structural condition assessment of the East Bay bridge in Gibsonton, Florida is evaluated with the help of remote health monitoring techniques. The bridge is a four-span, continuous, deck-type reinforced concrete structure supported on prestressed pile bents, and is instrumented with smart Fiber Optic Sensors. The sensors used for remote health monitoring are the newly emerged Fabry-Perot (FP), and are both surface-mounted and embedded in the deck. The sensing system can be accessed remotely through fast Digital Subscriber Lines (DSL), which permits the evaluation of the bridge behavior under live traffic loads. The bridge was open to traffic since March 2005, and the collected structural data have been continuously analyzed since. The data revealed an increase in strain readings, which suggests a progression in damage. Recent visual observations also indicated the presence of longitudinal cracks along the bridge length. After the formation of these cracks, the sensors readings were analyzed and used to extrapolate the values of the maximum stresses at the crack location. The data obtained were also compared to initial design values of the bridge under factored gravity and live loads. The study showed that the proposed structural health monitoring technique proved to provide an efficient mean for condition assessment of bridge structures providing it is implemented and analyzed with care.

• JSTS:Journal of Semiconductor Technology and Science
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• v.5 no.1
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• pp.30-37
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• 2005

We present an air bridge type electrode system with tunable electrode distance for detecting electroactive biomolecules. It is known that the narrower gap between electrode fingers, the higher sensitivity in IDA (interdigitated array) electrode. In previous researches on IDA electrode, narrower patterning required much precise and expensive equipment as the gap goes down to nanometer scale. In this paper, an improved method is suggested to replace nano gap pattering with downsizing electrode distance and showed that the patterning can be replaced by thickness control using metal deposition methods, such as electroplating or metal sputtering. The air bridge type electrode was completed by the following procedures: gold patterning for lower electrode, copper electroplating, gold deposition for upper electrode, photoresist patterning for gold film support, and copper etching for space formation. The thickness of copper electroplating is the distance between upper and lower electrodes. Because the growth rate of electroplating is $0.5{\mu}m\;min^{-1}$, the distance is tunable up to hundreds of nanometers. Completed electrodes on the same wafer had $5{\mu}m$ electrode distance. The gaps between fingers are 10, 20, 30, and $40{\mu}m$ and the widths of fingers are 10, 20, 30, 40, and $50{\mu}m$. The air bridge type electrode system showed better sensitivity than planar electrode.

• The Bulletin of The Korean Astronomical Society
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• v.39 no.1
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• pp.84.1-84.1
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• 2014

We report the spatial distribution of early-type stars and pre-main-sequence (PMS) stars around the starburst type young open cluster Westerlund 2. The early-type were selected from UBVI photometric data, while the PMS members were identified from their X-ray emission and mid-infrared excess. The northern clump of the cluster is composed mainly of PMS stars detected in both optical and X-ray and seems to be coeval to the cluster, while PMS stars in the bright bridge region are highly obscured in optical wavelength. The bright bridge appear to be an on-going star forming region possibly triggered by the strong radiation field from both sides-massive stars in Westerlund 2 and WR 20b. We also found that there are many early-type stars not only in the cluster but also farther from the cluster up to several times of the cluster radius. These early-type stars are well aligned from east to southwest of the cluster. We conclude these early-type stars are members of an OB association in the RCW 49 nebula. This report indicates there is a complex star formation history in Westerlund 2 and its surrounding H II region, the RCW 49 nebula.

• Journal of Microbiology and Biotechnology
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• v.23 no.1
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• pp.7-14
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• 2013

In the Bacillus amyloliquefaciens ${\alpha}$-amylase (BAA), the loop (residues 176-185; region I) that is the part of the calcium-binding site (CaI, II) has two more amino acid residues than the ${\alpha}$-amylase from Bacillus licheniformis (BLA). Arg176 in this region makes an ionic interaction with Glu126 from region II (residues 118-130), but this interaction is lost in BLA owing to substitution of R176Q and E126V. The goal of the present work was to quantitatively estimate the effect of ionic interaction on the overall stability of the enzyme. To clarify the functional and structural significance of the corresponding salt bridge, Glu126 was deleted (${\Delta}$E126) and converted to Val (E126V), Asp (E126D), and Lys (E126K) by site-directed mutagenesis. Kinetic constants, thermodynamic parameters, and structural changes were examined for the wild-type and mutated forms using UV-visible, atomic absoption, and fluorescence emission spectroscopy. Wild-type exhibited higher $k_{cat}$ and $K_m$ but lower catalytic efficiency than the mutant enzymes. A decreased thermostability and an increased flexibility were also found in all of the mutant enzymes when compared with the wild-type. Additionally, the calcium content of the wild-type was more than ${\Delta}E126$. Thus, it may be suggested that ionic interaction could decrease the mobility of the discussed region, prevent the diffusion of cations, and improve the thermostability of the whole enzyme. Based on these observations, the contribution of loop destabilization may be compensated by the formation of a salt bridge that has been used as an evolutionary mechanism or structural adaptation by the mesophilic enzyme.

• Journal of the Korean Graphic Arts Communication Society
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• v.22 no.1
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• pp.83-94
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• 2004

PSR ink is used to insulation coating material that heat resistance is. The use purpose is used for bridge prevention, circuit protection, stabilization of insulation. Heat-cured resin was used mainly on the materials of PSR inks. But, UV-curing type resin in used. Also, because of recently environmental problem, ink is going to water type. Purpose of this study is to develop PSR ink that can develop in pure water. and experiment did that do from that find suitable oligomer and monomer and does brand ratio differ. Specially Knew that is extent water soluble UV resin develop possible is DPHA 10~50% that A/A1924 is 50~90wt %, monomer. As a result, when ratio of A/A1924 and DPHA low viscosity epoxy resin is 5:1.5:1.5, could get high sensibility pattern repeatability, tack and alkali-resistance.

• Water for future
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• v.6 no.1
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• pp.19-28
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• 1973

Presented is the results of a model study about scouring around a bridge pier. 24 different types of piers were inestigated for the depth and width of scouring. The grain size of sand used in the experiment ranges from 0.149 to 2, 380mm, The time required to reach the stable bed formation was about 30min, with the range of Froud number from 0.58 to 0.69. It has been found that the scouring is most severe at the frontal side of the pier and decreases with decreasing a contact angle (between the center line of the pier and tangent to the frontal face). For design purposes, it is recomanded that the contact angle at the frontal face is 25 degrees for a triangular type and 40 deyrees for a circular type respectively.

• Journal of fish pathology
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• v.25 no.1
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• pp.11-20
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• 2012

C-type lectins are crucial for pathogen recognition, innate immunity, and cell-cell interactions. In this study, a C-type lectin gene was cloned from the rock bream. The full-length RbCTL cDNA was 729 bp with a 429 bp ORF encoding a 164-residue protein. The deduced amino acid sequence of RbCTL had all of the conserved features crucial for its fundamental structure, including the four cysteine residues involved in sulfide bridge formation and potential $Ca^2+$/carbohydrate-binding sites. RbCTL contains a signal peptide one single carbohydrate recognition domain. It showed 29.4% similarity to the C-type lectin of rainbow trout. RbCTL mRNA was predominately expressed in gill and head-kidney tissue and expressed less in peripheral blood leukocytes, trunk-kidney, spleen, liver, intestine and muscle. Expression of RbCTL was differentially upregulated in rock bream stimulated with LPS, Con A/PMA and poly I:C.

• Journal of the Korea institute for structural maintenance and inspection
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• v.12 no.1
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• pp.183-192
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• 2008

Generally, application of steel pipe pile as deep foundation member needs special requirement for the connection method between steel pipe pile and concrete footing. Even though two types of connection method are suggested in the korea highway bridge code, type-B method is prevalent. In this study, vertical, lateral, and tension loading test are done for two types of type B connection to review stress concentration, formation and behavior of imaginary RC column in the footing. Welding type and hook type as the connection method are considered in this study. Test results show that welding type have the more reserve capacity than hook type and the specimens connected by the welding type behave as the imaginary RC column in the footing. However, the specimens connected by the hook type did not behave as the imaginary RC column in the footing but behave as the hinge.

• Journal of Microbiology and Biotechnology
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• v.20 no.2
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• pp.301-310
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• 2010

Bacillus subtilis strains produce a broad spectrum of bioactive peptides. The lipopeptide surfactin belongs to one well-known class, which includes amphiphilic membrane-active biosurfactants and peptide antibiotics. Both the srfA promoter and the ComP-ComA signal transduction system are an important part of the factor that results in the production of surfactin. Bs-M49, obtained by means of low-energy ion implantation in wild-type Bs-916, produced significantly lower levels of surfactin, and had no obvious effects against R. solani. Occasionally, we found strain Bs-M49 decreased spore formation and the development of competence. Blast comparison of the sequences from Bs-916 and M49 indicate that there is no difference in the srfA operon promoter PsrfA, but there are differences in the coding sequence of the comA gene. These differences result in three missense mutations within the M49 ComA protein. RT-PCR analyses results showed that the expression levels of selected genes involved in competence and sporulation in both the wild-type Bs-916 and mutant M49 strains were significantly different. When we integrated the comA ORF into the chromosome of M49 at the amyE locus, M49 restored hemolytic activity and antifungal activity. Then, HPLC analyses results also showed the comA-complemented strain had a similar ability to produce surf actin with wild-type strain Bs-916. These data suggested that the mutation of three key amino acids in ComA greatly affected the biological activity of Bacillus subtilis. ComA protein 3D structure prediction and motif search prediction indicated that ComA has two obvious motifs common to response regulator proteins, which are the N-terminal response regulator receiver motif and the C-terminal helix-turn-helix motif. The three residues in the ComA N-terminal portion may be involved in phosphorylation activation mechanism. These structural prediction results implicate that three mutated residues in the ComA protein may play an important role in the formation of a salt-bridge to the phosphoryl group keeping active conformation to subsequent regulation of the expression of downstream genes.