• 한국버섯학회지
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• 제16권3호
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• pp.140-146
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• 2018

Pleurotus eryngii is one of the most commercially important mushrooms cultivated in Korea. However, the shelf-life of the fruiting body is short, limiting its export. A new hybrid strain H17 of P. eryngii was developed to extend the shelf-life by mono-mono crossing between monokaryotic strains derived from DanBi and KNR2774. Although the cultivation period of H17 was slightly longer than that of the reference cultivar Kenneutari No.2, the quality did not change and remained normal after a period of 65.0 days at $4^{\circ}C$. This result was significantly different from that of the reference cultivar Kenneutari No.2. Analysis of the genetic characteristics of the new hybrid strain H17 revealed a different profile from that of the parental and reference cultivars when random amplification of polymorphic DNA (RAPD) primers was used. These results demonstrate that H17 is a new cultivar with improved storability after harvesting.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 1991년도 춘계학술발표대회 논문집
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• pp.237-242
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• 1991

In order to increase the production of glutathione by maximizing the expression of recombinant gsh plasmids, two genes responsible for the biosynthesis of glutathione were cloned. A gshI gene was cloned onto pBR322 plasmid as 3.6Kb PstI DNA fragment from E. coli K-12 chromosomal DNA. Also gshII gene was cloned onto pUC13 plasmid as 2.2Kb PstI-BamHI DNA fragment. In order to improve the glutathione producing activity more efficiently, various recombinant plasmids containing tandem repeated gshI genes or both genes in various copy number onto the same vector were constructed. E. coli cells harboring pGH501 plasmid (pUC8-gshI$\cdot$I$\cdot$II) showed the highest glutathione synthesizing activity. The conditions for glutathione production with an ATP-generating system such as acetate kinase reaction of E. coli cells or glycolytic pathway of yeast cells were examined using the E. coli cells harboring the pGH501 plasmid. When the acetate kinase reaction of E. coli cells was used as an ATP generating system, 20mM of L-csteine was converted into glutathione with a yield of $100\%$.

• Applied Biological Chemistry
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• 제32권2호
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• pp.148-153
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• 1989

한국재래식된장에서 분리된 특향생성 Bacillus sp.의 NTG 변이주를 모균주로 하여 자외선에 의해 다시 돌연변이시킴으로써 amylase, protease의 증강과 동시에 deaminase가 감소되는, 한국된장의 발효시간 단축과 품질을 향상시킬 수 있는 우량변이주를 육종할 수 있었다. Amylase의 경우는 4.4배, protease의 경우는 4.2배까지 동시에 증강시킬 수 있는 우수한 변이주를 얻을 수 있었으며 두 효소 동시증강균주 중 deaminase 생성을 90%까지 저하시키는 한 균주를 개발할 수 있었다. 한편 각 변이주와 모균주와의 효소학적 특성은 거의 동일하였으며 고농도의 식염용액에서도 효소생산 및 작용에 큰 영향을 받지 않았다.

• International Journal of Industrial Entomology and Biomaterials
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• 제13권2호
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• pp.109-112
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• 2006

The use of commercial silkworm hybrids resistant to important silkworm diseases is economical and better option particularly in tropical areas. This necessitated the evolution of productive bivoltine silkworm breeds non-susceptible to $BmDNV_1$. Non-susceptibility to $BmDNV_1$, infection was found to be controlled by a single recessive gene, nsd-l or a dominant gene, Nid-l. A major dominant/recessive gene confers resistance to $BmDNV_1$, from potent donor parents have been transferred to 10 productive but susceptible bivoltine silkworm strains through conventional breeding methods. By utilizing these breeds prepared 25 hybrids $(5{\times}5)$ and hybrid evaluation was carried out to identify most promising hybrids resistant to $BmDNV_1$. All these hybrids are inoculated with $BmDNV_1$ inoculum along with productive control hybrid $CSR2{\times}CSR4$ and reared under standard rearing procedure. Based on inoculated rearing and test reeling results, two most promising hybrids $(CSR18DR{\times}CSR29DR\;and\;CSR21DR{\times}CSR50DR)$ were selected for commercial exploitation. The selected hybrids have shown a survival rate of >85% with productive traits, where as control hybrid have shown 11.1% survival with inferior cocoon traits. The methodologies adopted were discussed.

• 한국동물위생학회지
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• 제30권3호
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• pp.363-374
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• 2007

A biochemical characterization and antimicrobial drugs susceptibility study was conducted in four breeding kennel which was canine abortion caused by Brucella canis in Gyeongbuk province in 2003-2006. Total of 267 dogs domesticated in the four kennel were examination. Among them, 143 (53.6%) dogs were sero-positive and 25 of blood samples were isolated to Brucella canis. At amplification of 35KDa-BCSP gene using PCR, 711 bp DNA fragment was same visible in 25 isolates and B canis RM6/66. Biochemical characterization of B canis isolated was non-hemolytic, no production of $H_2S$, no fermentation of carbohydrates, catalase-positive, oxidase-positive, indol-negative, hydrolyzation of urea, reduction of nitrate and development of thionin dye medium. Using disk-diffusion method, all of 25 strains tested were found to be highly susceptible to tetracycline, aminoglycoside, quinolone, macrolide antibiotics, rifampin and ampicillin in vitro. Using PFGE with restriction enzyme Smi I, 25 isolates tested were typed to 2 pattern, S1 and S2.

• The Plant Pathology Journal
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• 제35권1호
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• pp.1-10
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• 2019

Wheat blast occurred in Bangladesh for the first time in Asia in 2016. It is caused by a fungal pathogen, Magnaporthe oryzae Triticum (MoT) pathotype. In this review, we focused on the current status of the wheat blast in regard to host, pathogen, and environment. Despite the many efforts to control the disease, it expanded to neighboring regions including India, the world's second largest wheat producer. However, the disease occurrence has definitely decreased in quantity, because of many farmers chose to grow alternate crops according to the government's directions. Bangladesh government planned to introduce blast resistant cultivars but knowledges about genetics of resistance is limited. The genome analyses of the pathogen population revealed that the isolates caused wheat blast in Bangladesh are genetically close to a South American lineage of Magnaporthe oryzae. Understanding the genomes of virulent strains would be important to find target resistance genes for wheat breeding. Although the drier winter weather in Bangladesh was not favorable for development of wheat blast before, recent global warming and climate change are posing an increasing risk of disease development. Bangladesh outbreak in 2016 was likely to be facilitated by an extraordinary warm and humid weather in the affected districts before the harvest season. Coordinated international collaboration and steady financial supports are needed to mitigate the fearsome wheat blast in South Asia before it becomes a catastrophe.

• The Plant Pathology Journal
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• 제39권2호
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• pp.220-227
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• 2023

Rose crown gall caused by Agrobacterium tumefaciens is a major disease that damages the production of cutroses in Korea. The effective prevention methods for this disease include the use of resistant varieties. This study was conducted to evaluate the resistance of 58 Korean cultivars and six foreign cultivars to crown gall disease with nodal explants in vitro. Among 180 A. tumefaciens strains, pathogenic strain RC12 was selected as an inoculant strain. The strain RC12 was identified based on characteristics of some selective media, pathogenicity test, and polymerase chain reaction analysis. Forty rose cultivars formed tumors on explants inoculated with A. tumefaciens RC12. However, 24 cultivars, including 22 Korean cultivars and 2 foreign cultivars, showed resistance to A. tumefaciens RC12 without forming any tumors. Six cultivars with tumor formation rates of over 30% formed initial tumors within 23 days after inoculation. Six cultivars with low tumor formation rates of around 5% formed initial tumors after 28 days of inoculation. It was found that gall formation rate was highly correlated with the initial gall formation period. Thus, the relationship between the period of gall formation and the rate of gall formation could be useful for assessing resistance to crown gall disease. In vitro inoculation methods could be used to evaluate resistance of cut-rose cultivars to crown gall diseases.

• 한국버섯학회지
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• 제13권2호
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• pp.129-134
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• 2015

느타리버섯(Pleurotus ostreatus)의 품종 육성을 위해서 는 많은 노력과 시간을 필요로 하고 있어, 최근에는 분자 육종방법으로 마커를 이용하여 빠르고 정확하게 교잡주를 선발하고 있다. 본 실험에서는 육종효율을 증진하기 위해 교잡효율이 높은 Di-Mon 교배방법으로 부친의 핵과 모친의 미토콘드리아를 가진 세포질전환 교잡주를 선발하였다. 느타리버섯 수한3호와 흑변이체의 교잡종인 '다굴'의 이핵균주에 춘추2호의 단핵균주와 교잡하여 120여개의 교잡주를 얻고 여기에 1차로 미토콘드리아 microsatellite DNA 마커 (MtPO1)를 이용하여 단핵균주 춘추2호의 미토콘드리아 밴드를 형성하는 교잡주 80개를 1차 선발하였다. 선발된 80균주중에서 URP 프라이머를 이용하여 '다굴'의 2핵균주 핵 DNA 패턴을 가진 26교잡주를 선발하였다. 이 중에서 수량성이 우수한 3개 교잡주의 자실체 특성을 조사한 결과 초발이일수가 2일이나 빠르면서 수량성이 좋은 품종을 선발하여 '천화심'이라 명명하였다. 천화심은 수량이 높으면서 여름에도 재배가 가능해 춘추2호의 약점을 보완함으로써 춘추대체품종으로도 재배가 가능하리라 본다.

• 한국약용작물학회지
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• 제3권1호
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• pp.25-29
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• 1995

작약주산지(芍藥主産地)의 일반농가재배(一般農家栽培) 포장(圃場)에서 수집(蒐集)된 지방종(地方種)의 지상부생육(地上部生育) 및 화기특성(花器特性)과 교배친화성(交配親和性)을 조사(調査)하여 교배육종(交配育種)의 기초자료(基礎資料)를 얻고자 시험(試驗)한 결과(結果)는 다음과 같다. 1. 작약(芍藥) 수집종(蒐集種)의 출현기(出現期)는 조기출현계통(早期出現系統)이 6종(種), 만기출현계통(晩期出現系統)이 13종(種)으로 구분(區分)되었다. 2. 작약(芍藥)의 화기특성중(花器特性中)화형(花形)은 홑꽃과 겹꽃으로 구분(區分)되었고, 화색(花色)은 홑꽃은 분홍색, 겹꽃은 분홍색+분홍색이 주종(主種)을 이루었고, 암술수는 꽃당 $3{\sim}4$개(倜)인 계통이 많았고, 수술수는 꽃당 $0{\sim}200$개(倜) 이상(以上)으로 차이가 많았으며, 화폭(花幅)은 10cm 미만(未滿)의 소형화(小形花)가 3종(種), 14cm 이상(以上)의 대형화(大型花)가 5종(種) 이었다. 3. 작약(芍藥) 수집종간(蒐集種間)의 인공교배(人工交配)에서 교잡(交雜) 친화성(親和性)은 대부분이 높은 것으로 검정(檢定)되었고 꽃당 골돌과(follicle)의 수(數)는 $3.0{\sim}5.0$개(倜), 종실(種實)의 수(數)는 평균(平均) 15.6개(倜)였으며, 인공교배(人工交配)에서 얻어진 종실(種實) 100입중(粒重)은 10g 이하(以下)인 것이 3조합(組合), 15g 이상(以上)인 것이 3조합(組合)이었으며 이들은 금후(今後) 육종 재료로 활용키 위해 계속 검토중에 있다.

• 한국균학회지
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• 제49권1호
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• pp.57-66
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• 2021

국립산림과학원이 개발한 표고 품종 6개를 대상으로 사각블럭배지에 적합한 품종을 선발하고자 하였다. 100일째 중량감소율은 평균 14.4% (12.8%~15.7%) 였다. 발생작업 후 5~7일에 발이조사 결과 배지당 2~36개였고, 만추향>산산향>산백향>밤빛향>산장향>태향고 순이었다. 대길이는 41~59 mm였고 태향고>산백향>밤빛향>만추향>산장향>산산향 순으로 길었다. 대길이가 짧고 대두께가 굵은 품종은 산산향이 우수하였다. 개체당 중량은 33~77 g였고 태향고>밤빛향>산산향>만추향>산장향>산백향 순이었다. 2 kg 배지의 생산량은 산백향이 가장 많은 582 g이었고 밤빛향>만추향>태향고>산산향>산장향 순이었다. 버섯 갓의 경도는 1,218~1,839 g/10 mm였고 태향고가 1,839 g/10 mm로 가장 단단하였고 밤빛향이 가장 낮았다. 결론적으로 사각블럭배지 재배조건에서 버섯 생산량은 산백향과 밤빛향이 좋았고, 버섯 형태면에서 산산향이 우수하였다.