• Korean Journal of Food Science and Technology
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• v.16 no.2
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• pp.149-152
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• 1984

Fifiy-five wheat samples were studied to obtain the basic information on the varietal differences of physicochemical characteristics and cookie baking potentiality in wheat breeding lines. Alkaline water retention capacity (AWRC) value had a correlation coefficient of $0.51^{**}$ with sedimentation value, of $0.45^{**}$ with protein content, and of $0.47^{**}$, with Pelshenke value. The highly significant correlation coefficient was obtained for cookie diameter vs. AWRC value $(-0.98^{**})$ and cooki diameter vs. protein content $(-0.46^{**})$. Farinograph absorption is highly correlated with AWRC value $(r=0.77^{**})$ and with cookie diameter $(r=-0.73^{**})$. Farinograph band width had a correlation coefficient of $-0.49^{**}$ with AWRC value, and of $0.44^{**}$ with cookie diameter.

• Journal of Plant Biotechnology
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• v.37 no.4
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• pp.567-573
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• 2010

To ascertain the effect of over-expressed maize calreticulin in tomato plant on tobamovirus movement in addition to validating potentiality of the gene (ZmCRT) as a means for the virus-resistance resource, four ZmCRT-expressing homozygous lines were generated from the T0 plants as using an Agrobacterium-mediated transformation, nucleic acid analyses, and a conventional breeding method. Of them, a line was subjected to the bioassay for tolerances to tobacco mosaic virus-U1 (TMV-U1) and tomato mosaic virus (ToMV) followed by RT-PCR and a chlorophyll fluorescence quenching analyses. Both transgenic plants transcribing ZmCRT and wild-type plants showed no symptom by 20 days after viruses inoculation, however the photosystem II quantum yield parameter measured from the upper leaves of ToMV-inoculated plants revealed that ZmCRT transgenic plants have higher photosynthetic ability than wild-type ones at that time, which indirectly implies that over-expressed ZmCRT product acts as a barrier to the cell-to-cell and/or systemic movement of ToMV. Moreover, ZmCRT transgenic plants showed remarkably longer shoot length than wild-type ones in 40 days after TMV-U1 or ToMV inoculation each, which might be resulted from higher photosynthetic ability during the phase not yet showing any external symptoms. Collectively, over-expressed ZmCRT protein in tomato plants is able to interrupt the systemic movement of infected TMV-U1 and ToMV even though not perfect.

• Proceedings of the Korean Society of Sericultural Science Conference
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• 1997.06a
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• pp.23-49
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• 1997

This is a progress report of rice biotechnology including development of gene transformation system, gene cloning and molecular mapping in rice. The scope of the research was focused on the connection between conventional breeding and biotech-researches. Plant transformation via Agrobacterium or particle bombardment was developed to introduce one or several genes to recommended rice cultivars. Two chimeric genes containing a maize ribosome inactivating protein gene (RIP) and a gerbicide resistant gene (bar) were introduced to Nipponbare, a Japonica cultivar, and transmitted to Korean cultivars. The homozygous progenies of herbicide resistant transgenic plant showed good fertility and agronomic characters. To explore the genetic resourses in rice, over 8,000 cDNA clones from immature rice seed have been isolated and sequenced. About 13% of clones were identified as enzymes related to metabolic pathway. Among them, twenty clones have high homology with genes encoding enzymes in the photorespiratory carbon cycle reaction. Up to now about 100 clones were fully sequenced and registered at EMBL and GenBank. For the mapping of quantitative tarits loci (QTL) and eternal recombinant inbred population with 164 F13 lines (MGRI) was developed from a cross between Milyang 23 and Gihobyeo, Korean rice cultivars. After construction of fully saturated RFLP and AFLP map, quantitative traits using MGRI population were analyzed and integrated into the molecular map. Eighty seven loci were determined with 27 QTL characters including yield and yield components on rice chromosomes. Map based cloning was also tried to isolate semi-dwarf (sd-1) gene in rice. A DNA probe, RG 109, the most tightly linked to sd-1 gene was used to screen from bacterial artifical chromosome (BAC) libraries and five over lapping clones presumably containing sd-1 gene were isolated. Rice genetic database including results of biotech reasearch and classical genetics is provided at Korea Rice Genome Server which is accessible with world wide web (www) browser. The server provides rice cDNA sequences and map informations linked with phenotypic images.

• The Journal of the Convergence on Culture Technology
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• v.4 no.4
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• pp.331-335
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• 2018

Zantedeschia spp. calla is very popular as a cut flower. It is very important to establish a micro propagation system through plant tissue culture with the problem that colored calla with various colors are low in natural reproduction rate and vulnerable to high temperature. In this study, we conducted the experiment by adding taurine to improve the growth of calla plant. When 20 mg/L of taurine was added with plant growth effect, 54.0 % of the cases of multiple shoots and 17.2 times of fresh weight were the most effective. Taurine 20 mg/L treatment showed 16.0 % and 39.2 %, respectively, than the untreated control. Taurine may contribute to mass propagation of elite breeding lines as well as an improvement of farm income by positively influencing the overall growth of calla plants, thereby positively affecting the establishment of the micro propagation system of calla shoot tips.

• Acta Via Serica
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• v.4 no.1
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• pp.87-109
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• 2019

In the Iranian epic $K{\bar{u}}shn{\bar{a}}ma$, there is a rather interesting story that recounts how the inhabitants of $B.s{\bar{i}}l\bar{a}$ cross-breed their domesticated horses with a magical horse living in the sea in order to obtain fine-bred ones. What is even more interesting is that similar accounts are also seen in many of other classical Perso-Arabic works and Chinese sources. The regions that such events took place in mainly spread over Central Asia and western China while in $K{\bar{u}}shn{\bar{a}}ma$, the story happens in $B.s{\bar{i}}l\bar{a}$, a legendary kingdom with its historical prototype being Silla. By sorting out certain records of how ancient people sought fine horses by cross-breeding domesticated horses with wild horses that inhabited mountains and waters within Chinese sources and classical Muslim works, and comparing these accounts with similar plot lines as depicted in $K{\bar{u}}shn{\bar{a}}ma$, this paper attempts to elucidate that the story in $K{\bar{u}}shn{\bar{a}}ma$ is a result of flourishing land and maritime exchanges between East Asia and West Asia during ancient and medieval times, rather than a purely literary fiction. It was not only influenced by the horse culture that thrived over the Eurasian Steppe, but the story is also coincidentally in accordance with the fact that the nomadic zone which lies within the central Eurasian continent extends as far as the Korean Peninsula in northeast Asia.

• Ocean and Polar Research
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• v.44 no.4
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• pp.365-372
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• 2022

The present study measured the growth performance of juvenile hybrid of red-spotted grouper ♀ × giant grouper ♂ (RGGG) across four water temperatures (19, 23, 27, and 31℃) and compared it to those of maternal purebreds (red-spotted grouper ♀ × ♂, RG) for eight weeks. The specific growth rate (SGR) and weight gain (WG) of RGGG increased as temperature increased, with greater SGR and WG at higher temperatures (27 and 31℃) than RG. The condition factor of RGGG was higher than that of RG and there were no differences between temperature groups within breeding lines. Food consumption increased at higher temperatures for both RGGG and RG, and was the highest in the 31℃ group. Similar to the SGR and WG, the food conversion rate (FCR) in RGGG decreased with increasing water temperature, with the significantly lowest value at 31℃; in RG, however, FCR progressively decreased in the 27℃ group, then increased in the 31℃ group. Furthermore, the FCR of the 31℃ group of RGGG, which had the lowest value among the RGGG groups, was lower than that of the 27℃ group of RG, which had the lowest RG value. In the analysis of nonlinear regression curves within the range of experimental temperature, the optimum temperature for SGR and FCR in RGGG was 31℃, unlike in RG. In conclusion, the growth performance of juvenile RGGG seems to have largely improved from the influence of paternal traits, hybrid with giant grouper, and it is presumed to be more profitable for commercial production in tropics/subtropics.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.5
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• pp.437-441
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• 1993

The improvement of superior black soybeans could be accomplished through maintenance and use of black soybean collections. A collection of 965 black soybean lines currently grown by farmers was made in 197 locations on spring, 1991. This study was carried out to evaluate them as useful germplasm. Seed characteristics of black soybean collections were evaluated for black soybean breeding for seed quality. The 100-seeds weight of 929 black soybean genotypes were distributed from 6.9 to 48.1 gram. The maximum and minimum 100-seeds weight were derived from Chungnam and Jeonbuk province, respectively. l00-seeds weight was heavier in middle and late maturity group, as maturity was later, whereas no tendency was shown in early maturity group. Length, width and thickness of the collected black soybean seeds were 9.5$\pm$1.5mm, 7.3$\pm1.3mm and 7.3$\pm1.2mm, respectively. Black soybean collections with yellow seed embryo was 72% and the others were green seed embryo.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.21 no.1
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• pp.82-86
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• 1976

'Radiation No. 6' the first mutant variety of naked barley in Korea was developed from Bangju as .a mother variety. The new variety was characterized by early maturity, short-culm and stablized high yield by the increased number of spikes. 'Radiation No. 6' was registered as a leading variety in 1974, .after 7 years since induction of mutation started.of mutation started.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.33-33
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• 2022

Rice (Oryza sativa L.) is a widely studied domesticated model plant. Seed awning is an unfavorable trait during rice harvesting and processing. Hence, awn was one of the target characters selected during domestication. However, the genetic mechanisms underlying awn development in rice are not well understood. In this study, we analyzed the genes for awn development using a mapping population derived from a cross between the Korean indica cultivar 'Milyang23' and NIL4/9 (derived from a cross between 'Hwaseong' and O. minuta). Two quantitative trait loci (QTLs), qAwn4 and qAwn9 were mapped on chromosome 4 and 9, respectively, increased awn length in an additive manner. Through comparative sequencing analyses parental lines, LABA1 was determined as the causal gene underlying qAwn4. qAwn9 was mapped to a 199-kb physical region between markers RM24663 and RM24679. Within this interval, 27 annotated genes were identified, and five genes, including a basic leucine zipper transcription factor 76 (OsbZIP76), were considered candidate genes for qAwn9 based on their functional annotations and sequence variations. Haplotype analysis using the candidate genes revealed tropical japonica specific sequence variants in the qAwn9 region, which partly explains the non-detection of qAwn9 in previous studies that used progenies from interspecific crosses. This provides further evidence that OsbZIP76 is possibly a causal gene for qAwn9. The O. minuta qAwn9 allele was identified as a major QTL associated with awn development in rice, providing an important molecular target for basic genetic research and domestication studies. Our results lay the foundation for further cloning of the awn gene underlying qAwn9.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.48-48
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• 2020

Fire blight, caused by Erwinia amylovora(Burrill), is a destructive disease of apple that damages blossoms, shoots, and woody plant organs. The fire blight disease is a worldwide problem for pome fruit growers because all popular apple cultivars are susceptible to the disease. Recently, fire blight of apple rootstocks has become a serious economic problem in high-density orchard systems in korea. The most commonly used dwarfing root stocks, M.9 and M.26, are highly susceptible to E. amylovora. The objective of the apple rootstock-breeding program has been to develop pomologically excellent rootstocks with resistance to abiotic and biotic stresses, including fire blight. Budagovsky 9 (B.9) apple rootstock is reported to be highly susceptible when inoculated with E. amylovora, although results from multiple trials showed that B.9 is resistant to rootstock blight infection in field plantings. So we tried to collect the apple rootstocks traits of fire blight resistance. The apple genotype Malus Robusta 5 (MR5) represents an ideal donor for fire blight resistance because it was described as resistant to all currently known European strains of the pathogen. The PCR for detecting the MR5 gene using the primers Md_MR5_FL_F/Md_MR5_FL_R. The results of these experiments confirmed some apple rootstocks traits of fire blight resistance showed the MR5. Furthermore, this gene is confirmed to be the resistance determinant of Mr5 as the transformed lines undergo the same gene-for-gene interaction in the host-pathogen relationship MR5-E. amylovora.