• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.6
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• pp.514-520
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• 2019

The reduction in performance due to inbreeding (i.e. inbreeding depression) has long been documented in plant and animal populations. The population of Korean native pigs are small and this breed is valuable in Korea.. This study was aimed to investigate effects of inbreeding depression on total number of piglets born (TNB) and number of piglets born alive (NBA) in Korean native pigs. We used 2,806 pedigree and 303 sows's data with 483 phenotypic records. After estimating genetic parameters for each traits, inbreeding depression was estimated using a mixed model in which the inbreeding coefficient was included as a covariate. Korean native pigs had high heritability for each traits. Inbreeding coefficient constantly increased from 1998 to 2017~2018 but there is no reduction for each traits in 2017~2018 in compared with those in 1998. Significant inbreeding depression was detected for TNB (p=0.03) but not for NBA (p=0.41). In addition there are significantly positive interactions between inbreeding coefficient and breeding value for both traits (p<0.05). These results suggest that Korean native pigs are still having genetic variation for TNB and NBA, which could overcome reproductive risks from inbreeding coefficient increase.

• Korean Journal of Plant Resources
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• v.34 no.4
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• pp.339-345
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• 2021

'Gondalbi' (Ligularia stenocephala (Maxim.) Matsum. & Koidz.) is the most cultivated Gomchwi species because of higher yield and low in aromatic flavor and bitter taste, But 'Gondalbi' is susceptible to powdery mildew disease and leaf shriveling after harvest in Summer. To improve powdery mildew disease resistance and post-harvest leaf shriveling problem in 'Gondalbi', 'Handeari-gomchwi' resistant to powdery mildew disease and having higher yield potential used as a paternal trait donor. Powdery mildew disease resistance and post-harvest leaf shriveling as well as agronomic performance of a new variety, 'Greenbear' were tested under field and green house conditions in Pyungchang, Korea from 2007 to 2016. Expression of both maternal and paternal characteristics in 'Greenbear' including purple colored petiole ears, glossy leaf and paternal, petiole trichome, absent at the back of a leaf were confirmed. Plant size and flowering time of 'Greenbear' were similar to check line, 'Gommany', while leaf number per plant and yield were higher in 'Greenbear'. 'Greenbear' has thinner leaves (0.66mm) compared to 'Gommany (0.69 mm)', and hardness appeared slightly higher in 'Greenbear(25.1 kg/cm²)'. The resistance to powdery mildew disease of the 'Greenbear' variety was slightly lower than that of the 'Gommany' variety, but somewhat showed high resistance.

• Animal Bioscience
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• v.34 no.7
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• pp.1202-1209
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• 2021

Objective: The intestinal microbiota plays an important role in host physiology, metabolism, immunity, and behavior. And host genetics could influence the gut microbiota of hybrid animals. The three-way cross model is commonly utilized in commercial pig production; however, the use of this model to analyse the gut microbial composition is rarely reported. Methods: Two three-way hybrid pigs were selected, with Saba pigs as the starting maternal pig: Duroc× (Berkshire×Saba) (DBS) pig, Berkshire×(Duroc×Saba) (BDS) pig. One hundred pigs of each model were reared from 35 days (d) to 210 d. The body weight or feed consumption of all pigs were recorded and their feed/gain (F/G) ratio was calculated. On day 210, 10 pigs from each three-way cross were selected for slaughter, and cecal chyme samples were collected for 16S rRNA gene sequencing. Results: The final body weight (FBW) and average daily gain (ADG) of DBS pigs were significantly higher than those of BDS pigs (p<0.05), while the F/G ratios of DBS pigs were significantly lower than those of BDS pigs (p<0.05). The dominant phyla in DBS and BDS pigs were Bacteroidetes (55.23% vs 59%, respectively) and Firmicutes (36.65% vs 34.86%, respectively) (p>0.05). At the genus level, the abundance of Prevotella, Roseburia, and Anaerovibrio in DBS pigs was significantly lower than in BDS pigs (p<0.01). The abundance of Eubacterium, Clostridium XI, Bacteroides, Methanomassiliicoccus, and Parabacteroides in DBS pigs was significantly higher than in BDS pigs (p<0.05). The FBWs and ADGs were positively correlated with Bacteroides, ClostridiumXI, and Parabacteroides but negatively correlated with the Prevotella, Prevotella/Bacteroides (P/B) ratio, Roseburia, and Anaerovibrio. Conclusion: These results indicated that host genetics affect the cecal microbiota composition and the porcine gut microbiota is associated with growth performance, thereby suggesting that gut microbiota composition may be a useful biomarker in porcine genetics and breeding.

• Journal of Animal Science and Technology
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• v.64 no.4
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• pp.717-726
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• 2022

The study evaluated different molt-inducing methods to achieve the main goal of molting in commercial layers during molting and post-molting periods. A total of 400 60-week-old Lohmann Brown layers were randomly divided into five groups (eight replicates of 10 birds for each group). Laying hens in the fasting control group received no diet from day 1 to day 10. The second group received a molt-inducing diet recommended by the breeding company. The third group received a wheat bran-based diet. The fourth group received a commercial layer diet with 8,000 ppm zinc (as zinc oxide, ZnO). The fifth group received an induced molting diet given to the second group with 8,000 ppm zinc, respectively. Egg production in the fasting control group and groups fed a diet with ZnO were significantly lower (p < 0.001) than those in groups fed the molt-inducing and wheat bran-based diets without ZnO during molting. Egg laying in the fasting control group was rapidly reduced and stopped on the 5.9th day of molting. In both groups having molt treatment with ZnO, egg production was similarly reduced and ceased on the 6.9th day and 7.0th day of molting, respectively, none of them differed significantly from the control. Layers fed molt-inducing diet or wheat bran-based diet did not reach the cessation of laying even on the 28th d of molting period. Relative weights of the ovary and growing oocytes of layers subjected to fasting or fed diets with ZnO were significantly lower than those of other groups. During the first two weeks of post molting, layers fed molt-inducing diet with ZnO showed higher egg production than the other two groups (p < 0.01). The eggshell strength in the group fed the commercial diet with ZnO was significantly higher than those fed the molt-inducing diet or wheat bran-based diets at 6 weeks of post molting (p < 0.05). These results suggest that the non-feed withdrawal molting using ZnO is more effective in inducing molting and increasing post-molt egg production and egg quality than other methods using a molt-inducing diet alone or wheat bran-based diet without ZnO.

• Korean Journal of Plant Resources
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• v.36 no.1
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• pp.91-99
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• 2023

Plus tree selection is the essential first step in a tree improvement program. This study was carried out to select plus trees of black locust (Robinia pseudoacacia L.) for tree improvement in Korea. The candidate plus trees of black locust were selected from twenty-one places in the four provinces (Gangwon, Gyeonggi, Chungcheong and Gyeongsang) following comparison selection. The trees were examined for qualitative and quantitative traits of economic interest like tree height (H), diameter at breast height (DBH), clear bole height (CBH), stem straightness (SS), crown size (CS) and disease resistance. The 64 selected trees showed higher quality in growth characteristics than control trees. Average of height, DBH, CBH, SS and CS were 20.5 m, 31.1 cm, 10.1 m, 1.2 and 5.2 m respectively. In particular, it was shown that the growth characteristics were higher in Chungcheong population. The superiority percentage of selected candidate plus trees of black locust over their respective check trees ranged from 7.3% to 18.9% in height, 20.0% to 40.6% in DBH, 32.8% to 73.5% in CBH, 42.9% to 55.2% in SS and -11.1% to 9.4% in CS, respectively. The selected black locust will be useful in future tree breeding programs based on their clonal and progeny performance.

• Korean Journal of Animal Reproduction
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• v.10 no.1
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• pp.49-57
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• 1986

Studies were conducted to investigate the relationship between hormone leveles and nutritional levels for improving performance of Cheju native cattle. In June 1984 a trial was initiated using 8 Cheju native calves after weaning, fed at two su, pp.ementary feeding levels (NRC 100% and 70%). The body weight, breedng performence, change in progesterone level during pregnancy and estrus cycle were evaluated. Mean body weight at 6 months of age was 155kg when fed 100% NRC ration but it was only 137kg when heifers received the 70% NRC ration. At 10, 15 and 20 months of age the body weight was 66, 160 and 115kg, respectively, showing that heifers fed the standard ration gained weight rapidly (P<0.01). Average size of the lefe ovary in the standard group was 2.1${\times}$1.6cm and right ovary was 2.6${\times}$1.8cm. However in the restricted feeding group the ovaries were found to be smaller. Diameter of graffian follicles showed a similar tendency to ovarian size in the two groups. The first oestrus in the standard feeding group a, pp.ared at 14.6 months when body weight was 265kg. Age at first calving was on average 28.9 months at a body weight of 436kg. On the other hand when heifers were fed the restricted ration the first oestrus a, pp.ared at 23.0 months at a body weight of 250kg. Average age at first calving was 38.9 months which was 10 months later than the average in the standard feeding group (P<0.01). In standard feeding group the progesterone level was 2.0ng/ml at two weeks after pregnancy and gradully increased up to 4 weeks and peaked at 18 weeks. This peak (6.4-6.5ng/ml) was maintained up to 24 weeks when progesterone level decreased until it reached 2.1ng/ml at the end of pregnancy. In the restricted group progesterone level up to 16 weeks followed a similar pattern to the standard group but there was a tendency in the restricted group to have lower progesterone levels(P<0.01). The standard and srstricted groups showed similar patterns of progesterone concentration during the oestrus cylce. There were no statistically significant differences in progesterone levels between standard and restricted groups but there was variation between induvidual animals.

• Journal of Microbiology and Biotechnology
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• v.33 no.6
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• pp.831-839
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• 2023

Tylosin is a potent veterinary macrolide antibiotic produced by the fermentation of Streptomyces fradiae; however, it is necessary to modify S. fradiae strains to improve tylosin production. In this study, we established a high-throughput, 24-well plate screening method for identifying S. fradiae strains that produce increased yields of tylosin. Additionally, we constructed mutant libraries of S. fradiae via ultraviolet (UV) irradiation and/or sodium nitrite mutagenesis. A primary screening of the libraries in 24-well plates and UV spectrophotometry identified S. fradiae mutants producing increased yields of tylosin. Mutants with tylosin yield 10% higher than the wild-type strain were inoculated into shake flasks, and the tylosin concentrations produced were determined by high-performance liquid chromatography (HPLC). Joint (UV irradiation and sodium nitrite) mutagenesis resulted in higher yields of mutants with enhanced tylosin production. Finally, 10 mutants showing higher tylosin yield were re-screened in shake flasks. The yield of tylosin A by strains UN-C183 (6767.64 ± 82.43 ㎍/ml) and UN-C137 (6889.72 ± 70.25 ㎍/ml) was significantly higher than that of the wild-type strain (6617.99 ± 22.67 ㎍/ml). These mutant strains will form the basis for further strain breeding in tylosin production.

• Proceedings of the Korean Society of Computer Information Conference
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• 2020.07a
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• pp.575-576
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• 2020

본 논문은 근래에 식용곤충 식품에 대한 수요 및 국민적 관심이 증가하여 관련 산업이 급격히 성장하고 있는 가운데, 건강기능성 효과가 널리 알려진 흰점박이꽃무지 유충의 안정적인 생산량 확보를 위한 스마트 사육사를 제작하고 그 성능을 평가한 결과이다. 사육사는 L6m×W3m×H2.8m 크기로 제작하였으며, 안정적인 사육환경을 위하여 사육실과 공조실을 분리하여 설계하였다. 공시재료는 생후 15일이 경과된 흰점박이꽃 무지 유충 1령이며, 스마트 사육사 내 사육환경은 온도 25±2℃, 습도 65±5%로 제어하였다. 사육조사는 매주 1회, 유충의 체중, 길이, 두께를 측정하였으며, 스마트 사육사의 성능평가를 위해 일반 사육농가(전북 소재)와 비교·분석하였다. 사육 4주 후 조사 결과, 스마트 사육사에서 사육한 유충의 체중과 길이는 각각 평균 1.97g/마리와 3.75cm로, 일반농가의 1.58g/마리와 3.55cm에 비해 비교적 높은 것으로 나타났다. 하지만, 두께의 경우 2주 차까지 일반농가에서 대체로 높은 것으로 나타났으며, 이후 3~4주 차에서는 큰 차이를 보이지 않았다. 따라서 본 연구를 통해 개발한 흰점박이꽃무지 유충 스마트 사육사는 일반농가와 비교해 사육이 비교적 빠르고 생산량을 더 많이 확보할 수 있는 시스템으로 농가소득 증대에 유용할 것으로 판단되며, 장소 및 시간에 상관없이 생육환경 제어가 가능하여 개발된 시제품의 보급 확대가 필요하다.

• Animal Bioscience
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• v.37 no.5
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• pp.952-961
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• 2024

Objective: Stocking density (SD) is an important issue in the poultry industry, which is related to the production performance, intestinal health and immune status. In the present study, the effects of SD on the metabolism and homeostasis of uric acid as well as the related functions of the liver and kidney in ducks were examined. Methods: A total of 360 healthy 56-day-old Shan-ma ducks were randomly divided into the low stocking density (n = 60, density = 5 birds/m²), medium stocking density (n = 120, density = 10 birds/m²) and high stocking density groups (HSD; n = 180, density = 15 birds/m²). Samples were collected in the 3rd, 6th, and 9th weeks of the experiment for analysis. Results: The serum levels of uric acid, lipopolysaccharide and inflammatory cytokines (interleukin-1β [IL-1β], IL-8, and tumor necrosis factor-α [TNF-α]) were increased significantly in the HSD group. Serious histopathological lesions could be seen in both the livers and kidneys in the HSD group in the 9th week. The mRNA expression levels of inflammatory cytokines (IL-8 and TNF-α) and related pathway components (toll-like receptor 4, myeloid differentiation primary response gene 88, and nuclear factor-κB) were increased significantly in both the livers and kidneys in the HSD group. The mRNA expression levels of enzymes (adenosine deaminase, xanthine oxidase, phosphoribosyl pyrophosphate amidotransferase, and phosphoribosyl pyrophosphate synthetase 1) related to the synthesis of uric acid increased significantly in the livers in the HSD group. However, the mRNA expression level of solute carrier family 2 member 9, which plays an important role in the excretion of uric acid by the kidney, was decreased significantly in the kidneys in the HSD group. Conclusion: These results indicated that a higher SD could cause tissue inflammatory lesions in the liver and kidney and subsequently affect the metabolism and homeostasis of uric acid, and is helpful for guiding decisions related to the breeding and production of ducks.

• Korean Journal of Poultry Science
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• v.40 no.4
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• pp.351-359
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• 2013

This study was undertaken to assess dietary crude protein (CP) and amino acid (AA) concentrations for growth performance and carcass characteristics in Korean native ducks. In a $2{\times}3$ factorial arrangement, 1-d-old Korean native male ducks were allotted to 6 dietary treatments in a completely randomized design. Experimental diets contained 23 or 21% CP with 1.31/1.09, 1.21/1.00, 1.11/0.91 and 1.11/0.91, 1.02/0.83, 0.92/0.75 AA (Lysine/Total sulfur amino acid, Lysine/TSAA), respectively, from 0 to 3 wk of age. From 4 to 8 wk of age, experimental diets had 19 or 17% of diet; each contained 1.00/0.79, 0.94/0.75, 0.88/0.71 and 0.88/0.71, 0.82/0.67, 0.76/0.63 AA (Lysine/TSAA), respectively. Each dietary treatment has 6 replicates and feed and water were provided ad libitum. Body weight (BW), feed intake and uniformity were measured at 3 wk and 8 wk and carcass characteristics were evaluated at 8 wk of age. As CP increased from 21 to 23%, the BW and BW gain significantly increased (P<0.05) during 0 to 3 wk of age. From 4 to 8 wk of age, BW, feed intake, BW gain, feed conversion rations (FCR) and uniformity were not different (P>0.05) between treatments. Carcass yield and relative weights of liver, spleen, right breast and leg per 100 of BW were not different (P>0.05) between treatments. The meat color, shear force value, cooking loss and pH were not affected by dietary treatments (P<0.05). Korean native ducks require relatively low levels of dietary CP and AA for late growth and carcass yield due to low daily weight gain. This suggests the possible differences in CP and AA needs between Korean native ducks and commercial breeds from foreign breeding companies, especially late growth stage.