• Korean Journal of Environment and Ecology
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• v.31 no.2
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• pp.127-134
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• 2017

This study was conducted to identify the reproductive behavior of the barn swallow. The study was carried out in Gwangju, Korea during the 2013 breeding season. In the morning, the nest-building frequency was 14.2~31.0 trips/h (10.2~19.8 trips/h by male and 4.0~11.4 trips/h by female). The nest-building activity took 7.6~15.9 min/h (4.8~8.1 min/h by male and 2.8~8.0 min/h by female). The nest-building time by female ($40.0{\pm}27.9sec/trip$) was about 1.5 times longer than the nest-building time by male ($26.1{\pm}15.5sec/trip$). Only the female incubated the eggs. The Incubation time was $50.6{\pm}17.5min/h$ (84%) at 6h, 24.5 min/h (40.8%) at 7h and 15.6 min/h (26.0%) thereafter. During daytime, the female incubation time showed a highly significant difference (p<0.001), and the incubation time at 6h was higher than that at other times. There was a significantly negative correlation between female incubation time and the mean air temperature(p<0.05). The frequency of feeding was $385.2{\pm}66.9trips/nest$ in the daytime ($219.2{\pm}37.1trips/nest$ by male and $166.0{\pm}30.8trips/nest$ by female). The frequency of feeding per hour was $32.1{\pm}12.3trips/h$ ($18.3{\pm}7.8trips/h$ by male and $14.3{\pm}4.5trips/h$ by female). The frequency of feeding per hour showed a significant difference in the range of 10h(p<0.05) and 15h(p<0.01) by sex. The time of feeding by female ($40.9{\pm}83.3sec/trip$) was longer than the time of feeding by male ($12.3{\pm}31.0sec/trip$). The juvenile defecation frequency was $45.6{\pm}8.4times/nest$ per day and showed a positive correlation with feeding frequency (p<0.05). The results of this study will be helpful in understanding the reproductive behaviors of the swallow adapted to the environment in Korea.

• The Plant Pathology Journal
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• v.22 no.3
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• pp.265-270
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• 2006

Cultivated hot pepper crops showing severe mosaic symptom were found in Korea in 2004 and their causal agent was identified as Cucumber mosaic virus (CMV). These pepper crops was resistant to the virus in the filled, and they belonged to pathotype 0 (P0) resistant pepper. Resistance screening of selected pepper plants showed that a pepper isolate of CMV was the P0 resistance-breaking virus. This P0 resistance-breaking isolate of CMV, named as Ca-P1, was isolated from leaves of the virus-infected Capsicum annuum cv. Manidda that showed systemic severe mosaic symptom. Ca-P1-CMV could induce systemic mosaic symptoms on P0-susceptible (P0-S) and P0-resistant (P0-R) cultivars whereas an ordinary strain (Fny-CMV) could not infect P0-R. This result suggests that Ca-P1-CMV can overcome P0 resistant pepper cultivars. To analyze its genome sequence, the complete nucleotide sequence of RNA3 of Ca-P1-CMV was determined from the infectious full-length cDNA clone of the virus. RNA3 of Ca-P1-CMV consisted of 2,219 nucleotides. Overall sequence homology of RNA3-encoded two viral proteins (movement protein and coat protein) revealed high similarity (75.2-97.2%) with the known CMV strains. By sequence analysis with known representative strains of CMV, Ca-P1-CMV belongs to a typical member of CMV subgroup IB. The resistance and resistance-breaking mechanisms of pepper and counterpart CMV, respectively, remain to be investigated, which will enrich the genetic resources and accelerate CMV-resistant pepper breeding programs.

• Molecules and Cells
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• v.38 no.9
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• pp.773-780
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• 2015

3D8 single chain variable fragment (scFv) is a recombinant monoclonal antibody with nuclease activity that was originally isolated from autoimmune-prone MRL mice. In a previous study, we analyzed the nuclease activity of 3D8 scFv and determined that a HeLa cell line expressing 3D8 scFv conferred resistance to herpes simplex virus type 1 (HSV-1) and pseudorabies virus (PRV). In this study, we demonstrate that 3D8 scFv could be delivered to target tissues and cells where it exerted a therapeutic effect against PRV. PRV was inoculated via intramuscular injection, and 3D8 scFv was injected intraperitoneally. The observed therapeutic effect of 3D8 scFv against PRV was also supported by results from quantitative reverse transcription polymerase chain reaction, southern hybridization, and immunohistochemical assays. Intraperitoneal injection of 5 and $10{\mu}g$ 3D8 scFv resulted in no detectable toxicity. The survival rate in C57BL/6 mice was 9% after intramuscular injection of 10 $LD_{50}$ PRV. In contrast, the 3D8 scFv-injected C57BL/6 mice showed survival rates of 57% ($5{\mu}g$) and 47% ($10{\mu}g$). The results indicate that 3D8 scFv could be utilized as an effective antiviral agent in several animal models.

• Korean Journal of Environmental Biology
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• v.27 no.4
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• pp.369-374
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• 2009

The stumpy bullhead (Pseudobagrus brevicorpus) endemic to Korea is one of critically endangered freshwater fish species. To provide baseline data for its captive breeding and reintroduction we investigated basal characteristics on its growth and sexual maturation in an artificial rearing environment. After 698-day rearing the stumpy bullhead grew up to $89.22{\pm}10.29\;mm$ in total length (TL) and $70.93{\pm}7.68\;g$ in total body weight (BW)($BW=5\times10^{-5}\;TL^{2.678}$; $R^2=0.995$), and showed survival rate of 74.2%. The sex ratio was 1 (471 females) : 1 (473 males), and males ($TL=87.44{\pm}1.07mm$; $BW=8.43{\pm}0.31\;g$) showed significantly faster growth than females ($TL=78.38{\pm}0.92mm$; $BW=6.38{\pm}0.20\;g$) after 663-day rearing. However, condition factor of females was significantly higher than that of males (P<0.05). The number of eggs per female ranged from 508 to 867 (average 734), and their diameters ranged from 0.04 to 1.65 mm. The females which reared about two years in the laboratory condition was able to spawn in the early June after 1~2 days of human chorionic gonadotropin (HCG) injection. The number of hatched fries ranged from 113 to 338 (average 216).

• Journal of Environmental Impact Assessment
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• v.23 no.2
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• pp.75-87
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• 2014

In order to analyze environmental impact of livestock manure and organic fertilizers, this study investigated livestock-breeding and pollution loads, the status of individual and public livestock manure treatment facilities, and the status of production, supply and components of compost and liquid fertilizers in the Nonsan area. Also, on a trial basis, this study investigated the life cycle of the environmental impact of livestock manure and its organic fertilizers on stream, groundwater, and agricultural soil. The results are as follows. Firstly, were detected the range of $0.13{\sim}1.32{\mu}g/L$ of As, $0.004{\sim}0.467{\mu}g/L$ of Cd and $0.5{\sim}9.2{\mu}g/L$ of Pb as a harmful substances which show lower concentrations than person preservation criteria of water qualities and aquatic ecosystem. However, it is not clear that heavy metals affect environment such as stream, groundwater and agricultural soil. Secondly, this influence could change according to investigation time and treatment efficiency. As were detected large amounts of persistent organic pollutants(e.g. $14.24{\sim}38.47{\mu}g/L$ of acetylsalicylic acid, $1.17{\sim}2.96{\mu}g/L$ of sulfamethazine, and $2.25{\sim}174.09{\mu}g/L$ of sulfathiazole) in effluent from livestock farms and small amounts of sulfathiazole($ND{\sim}1.63{\mu}g/L$) in the stream, it is necessary to monitor POPs at individual and public livestock manure treatment facilities. However, significant environmental impact did not appear at groundwater and agricultural soil in the test area supplied with liquid fertilizers. These results could be applied to investigate the environmental impact of livestock manure through a comprehensive livestock manure management information system.

• Journal of the Korean Institute of Landscape Architecture
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• v.37 no.6
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• pp.39-47
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• 2010

The coastal area is the place where two conflicting values provide opportunities for recreation, tourism and conservation of the natural environment. Most of these coastal areas have been disregarded in terms of tourism because of the high value placed on natural resources. Management strategy has usually been first on the priority list of natural. resources. However, due to the significant number of visitors, the Important-Satisfaction Analysis(ISA) was applied as a mean of complement since the 1970s. This study analyzed the difference between visitor satisfaction and the importance of the main management strategy targeting visitors to Sun-cheon Bay Ecological Park on weekdays and weekends. Results show that Sun-cheon Bay Ecological Park and the city government have paid a lot of attention and invested a lot of money, but efficiency of publicity didn't come up to their afford. Therefore, we should prepare information facilities, public education facilities and human power. Also needed are visitors' temporal-spacial control to set specific programs and a guide for information education control. It means visitors' company forms change depends on weekday and weekend. In addition, a breeding space for birds should be built for observation, education and exhibition to help meet visitor expectations. Visitors' positive satisfaction might be provided in establishing strategy as a very important measure in limited area. In conclusion, this study might be provided as preliminary data when the management strategy and related guidelines are established through the management priority of coastal regions where importance and satisfaction conflict.

• Journal of Environmental Science International
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• v.23 no.8
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• pp.1447-1453
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• 2014

The differentiation of sex is important for species preservation. However, Fairy Pitta is sexually monomorphic and sex of an individual is indistinguishable with its external characteristics. We determined the sex of Fairy Pitta through DNA analysis and investigated the causes and time of injury and mortality and the size based on sex. We collected 21 samples at Jeju Island, Korean Peninsula from 2004 to 2013 and extracted DNA from them and amplified chromo helicase DNA-binding gene from Z and W chromosomes through Polymerase Chain Reaction (PCR). We confirmed their sex with the banding pattern through Agarose gel electrophoresis, i.e. male (ZZ): one banded and female (ZW) two banded. We distinguished the sex of 17 of 21 samples resulting in 9 males and 8 females. Most casualties were recorded in adult of both sexes. Causes of injury and mortality proved that female casualties occurred from window strikes, dehydration, car accident, predation by natural enemies, and male occurred from window strikes, car accident and dehydration. The time of injury and mortality in adults differ by sex. There was no difference between sexes in any of the six size parameters. As the time of injury and mortality differ by sex, the survey on the role and ecological nature by sex in breeding season must be carried out in the future. External measurements may not be reliable for sexing of Fairy Pitta and other traits such as vocal or characteristics are required to identify the sex of individuals in the field.

• Journal of Plant Biotechnology
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• v.32 no.1
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• pp.51-55
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• 2005

Chinese leymus (Leymus chinensis Trin.) is a perennial grass that is widely distributed at high pH sodic and arid soil in the northeastern Asia. An efficient regeneration system was established through somatic embryogenesis of mature seeds to understand its high adaptability to harsh environmental conditions on the basis of molecular biology. The calli were efficiently induced (about $70\%$) from mature seeds on MS medium supplemented with $1.5\;\cal{mg/L}$ 2,4-D. Somatic embryos were formed from the surface of embryogenic callus on MS medium supplemented with $2.0\;\cal{mg/L}\;kinetin\;and\;0.5\;\cal{mg/L}$ NAA after 3 weeks of culture. Roots were induced from the shoot when transferred to MS medium without plant growth regulator for 1 week. Plant regeneration rate was $36\%$ and regenerated plantlets were grown to normal mature plants in pot. An efficient plant regeneration system in this study will be useful for molecular breeding of L. chinensis.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.4
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• pp.361-374
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• 2011

The cereal grain crops have strong flexibility against adverse environment and they have various functional compounds. The objective of the present study was to screen phenolic compounds in sorghum [Sorghum bicolor (L.) Moench], foxtail millet (Setaria italica), common millet (Panicum miliaceum L.) by high performance liquid chromatography (HPLC) with photodiode array (PDA) detector. Sorghum contained the highest amount of phenolic compounds among three different crops (sorghum, foxtail millet, common millet). Especially Moktaksusu showed the highest amount of phenolic compounds concentrations and biggest regional differences. The comparison of average phenolic compounds in sorghums by regions showed order to Milyang ($963.3\;{\mu}g{\cdot}g^{-1}$), Yeongyang ($923.1\;{\mu}g{\cdot}g^{-1}$), Gijang ($831.3\;{\mu}g{\cdot}g^{-1}$) and Bonghwa ($735.6\;{\mu}g{\cdot}g^{-1}$). Among the sorghum cultivars, Moktaksusu ($1407.9\;{\mu}g{\cdot}g^{-1}$) had the highest concentration of phenolic compounds. The average phenolic compounds of foxtail millets showed similar amount among Milyang ($319.0\;{\mu}g{\cdot}g^{-1}$), Gijang ($288.1\;{\mu}g{\cdot}g^{-1}$) and Bonghwa ($281.9\;{\mu}g{\cdot}g^{-1}$) areas. The phenolic compounds of Yeongyang ($246.6\;{\mu}g{\cdot}g^{-1}$) slightly low and that showed similar concentrations among three different regions. The concentration of phenolic compounds in foxtail millets, Chungchajo ($335.6\;{\mu}g{\cdot}g^{-1}$) showed the highest concentrations. The average phenolic compounds of common millets showed the highest concentrations in Milyang ($305.5\;{\mu}g{\cdot}g^{-1}$), Bonghwa ($262.0\;{\mu}g{\cdot}g^{-1}$), Gijang ($195.1\;{\mu}g{\cdot}g^{-1}$), Yeongyang ($237.2\;{\mu}g{\cdot}g^{-1}$) in decreasing order. The concentration of phenolic compounds of common millets was the highest in the Norangchalgijang ($337.0\;{\mu}g{\cdot}g^{-1}$), Hwanggumgijang ($250.0\;{\mu}g{\cdot}g^{-1}$) was also relatively higher than others. The results of this study will provide basic information for breeding sorghums, foxtail millets and common millets with higher phenolic compound concentrations.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.11 no.1
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• pp.55-60
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• 1993

Forest occupied a part of natural ecosystem carries out a role of purifying air, preserving water resource, prevention of the breeding and extermination, recreation areas and etc that preserve and for me one's living environment. In this study, the classification for management of this forest is performed with Landsat TM Image. The classes are decided needle-leaf trees, broad-leaf trees, farming land and grass land, and water. When the TM digital images are classified on computer, water is represented in 7∼13 D.N. of 4th band. But the others is appeared similar mostly specific values so that must be done image processing. When the images compounded 2ed band and 3ed band are processed with ratio of enhancement. Needle-leaf treas is represented in l18∼136 D.N. of 1st band, broad-leaf trees in 72∼91 D.N. of 3ed band, farm land and glass land in 96∼120 of 3ed band. Forest Information is classified with M.L.C, an image classification method. The errors of needle-leaf trees, broad-leaf trees, farm land and grass land, and water are appeared each －7.43, ＋1.89,＋7.58 and －2.04 as compared the digital image with investigation on the scene. Finally, these results are useful for classification of forest vegetation with Landsat TM Image.