• Journal of Animal Science and Technology
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• 제57권2호
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• pp.5.1-5.8
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• 2015

Background: Korean native chicken (KNC) is a well-known breed due to its superior meat taste. This breed, however, owing to a low growth rate, has a high market price. In order to overcome this disadvantage, the National Institute of Animal Science (NIAS) in Korea developed a commercial KNC breed, named Woorimatdag version 2 (WM2), an upgraded version of the Woorimatdag (WM1) breed and the WM2 was created by crossing the KNC with meat type breeds. This study aims to discriminate between WM2 and other chicken breeds using microsatellite (MS) markers. Methods: A total of 302 individuals from eight Korean chicken populations were examined. The genetic diversity and population structure analysis were investigated using Cervus, API-CALC, STRUCTURE, PowerMarker programs. Results: Based on heterozygosity and polymorphic information content (PIC) values, 30 MS markers were initially selected from 150 markers. The identified average number of alleles (Na), expected heterozygosity, and PIC values for the WM2 samples were 7.17, 0.741, and 0.682, respectively. Additionally, the paternity of individuals was assigned with a success rate of greater than 99% using 12 markers, the best minimum number of markers. The 12 selected markers contained heterozygosity and PIC values above 0.7 and probability of identity values around zero. Using these markers, the determined probability of identity (PI), $PI_{half-sibs}$, and $PI_{sibs}$ values were 3.23E-33, 5.03E-22, and 8.61E-08, respectively. Conclusions: WM2 is well differentiated with respect to other chicken breeds based on estimated genetic distances. The results presented here will contribute to the identification of commercial WM2 chicken in the market.

• Animal Bioscience
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• 제36권1호
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• pp.10-18
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• 2023

Objective: In this study, we aimed to position the Hungarian Merino among other Merinoderived sheep breeds, explore the characteristics of our sampled animals' genetic similarity network within the breed, and highlight single nucleotide polymorphisms (SNPs) associated with daily weight-gain. Methods: Hungarian Merino (n = 138) was genotyped on Ovine SNP50 Bead Chip (Illumina, San Diego, CA, USA) and positioned among 30 Merino and Merino-derived breeds (n = 555). Population characteristics were obtained via PLINK, SVS, Admixture, and Treemix software, within-breed network was analysed with python networkx 2.3 library. Daily weight gain of Hungarian Merino was standardised to 60 days and was collected from the database of the Association of Hungarian Sheep and Goat Breeders. For the identification of loci associated with daily weight gain, a multi-locus mixed-model was used. Results: Supporting the breed's written history, the closest breeds to Hungarian Merino were Estremadura and Rambouillet (pairwise F_ST values are 0.035 and 0.036, respectively). Among Hungarian Merino, a highly centralised connectedness has been revealed by network analysis of pairwise values of identity-by-state, where the animal in the central node had a betweenness centrality value equal to 0.936. Probing of daily weight gain against the SNP data of Hungarian Merinos revealed five associated loci. Two of them, OAR8_17854216.1 and s42441.1 on chromosome 8 and 9 (-log₁₀P>22, false discovery rate<5.5e-20) and one locus on chromosome 20, s28948.1 (-log₁₀P = 13.46, false discovery rate = 4.1e-11), were close to the markers reported in other breeds concerning daily weight gain, six-month weight, and post-weaning gain. Conclusion: The position of Hungarian Merino among other Merino breeds has been determined. We have described the similarity network of the individuals to be applied in breeding practices and highlighted several markers useful for elevating the daily weight gain of Hungarian Merino.

• 한국산학기술학회논문지
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• 제21권1호
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• pp.240-246
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• 2020

본 연구는 백우 품종 육성을 위해 분자생물학적 방법을 이용하여 유전적 특성을 파악하고 백우 품종을 식별하기 위한 백우 품종 특이적인 Single Nucleotide Polymorphism (SNP) 마커를 개발하기 위해 수행되었다. 한우 48두와 백우 22두의 혈액에서 추출된 DNA를 이용하여 Illumina Bovine HD 777K SNP chip으로 SNP genotyping을 실시하였다. 각 SNP의 Minor Allele Frequency (MAF) difference (한우와 백우의 차이 절대값)을 계산하고, Fisher's Exact test (Genotype)을 통해 MAF difference의 통계적 유의성(P-value)을 계산하였다. 품종 별 차이를 나타낼 수 있는 마커를 선발기준으로 MAF difference가 100%의 차이를 나타내는 SNP를 식별하였다. 이러한 유전적 차이를 보이는 9개의 단일염기다형성 마커(rs42125585, rs42125591, rs42125833, rs109461720, rs134735704, rs109447299, rs42164846, rs42160000 및 rs137353829)가 선발되었다. 선발된 마커들은 한우와 백우 특이적인 대립유전자를 가지고 서로 다른 대립유전자를 나타내고 있다. 이들 9개의 SNP 마커들을 이용하여 한우와 백우의 품종을 식별할 수 있음을 확인하였고, 이러한 결과들을 바탕으로 백우 품종 식별 마커 특허를 등록하였다. 백우는 원종인 한우에서 분리되어 한국재래종의 특성을 잘 나타내 주는 계통으로, 이러한 백우가 가지고 있는 유전적 특성 연구는 백우를 식별하고 품종으로서 육종하는데 사용되어 종축으로서의 가치 증진을 위한 기반 연구가 될 것으로 생각된다.

• Bulletin of the Korean Chemical Society
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• 제31권7호
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• pp.1902-1906
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• 2010

A microchip electrophoresis (ME) method was developed using a programmed field strength gradients (PFSG) for the single nucleotide polymorphism (SNP) based fast identification of cattle breeds. Four different Korean cattle (Hanwoo) and Holstein SNP markers amplified by allele-specific polymerase chain reaction were separated in a glass microchip filled with 0.5% poly(ethyleneoxide) ($M_r$ = 8 000 000) by PFSG as follows: 750 V/cm for 0 - 14 s, 166.7 V/cm for 14 - 31 s, 83.3 V/cm for 31 - 46 s, and 750 V/cm for 46 - 100 s. The cattle breeds were clearly distinguished within 45 s. The ME-PFSG method was 7 times and 5 times faster than the constant electric field ME method and the capillary electrophoresis- PFSG method, respectively, with a high resolving power ($R_s$ = 5.05 - 9.98). The proposed methodology could be a powerful tool for the fast and simultaneous determination of SNP markers for various cattle breeds with high accuracy.

• Asian-Australasian Journal of Animal Sciences
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• 제17권10호
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• pp.1338-1343
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• 2004

The Debao pony ear marginal tissue fibroblast cell line (NDPEM 2/2) was uccessfully established using either primary explant technique or collagenase technique. The characterizations of the cell line were identified as following: the cells were adherent and of density limitation; population doubling time (PDT) of cells made with the two techniques were 35.9 h and 48 h, respectively; chromosome analysis showed that the frequency of cell chromosome number to be 2n=64 was 91.3%-92.8%. Confirmed by isoenzyme analysis, this cell line had no cross- contamination. Tests for microbial contamination from bacteria, fungi, virus or mycoplasma were negative. This newly established cell line meets all the standard quality controls of ATCC. It will provide a precious genetic resource for the conservation of the Debao pony breed, as well as effective experimental material for genetic studies on Debao ponies.

• International Journal of Industrial Entomology and Biomaterials
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• 제23권2호
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• pp.239-243
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• 2011

Thirty five bivoltine breeds were acclimatized under controlled condition for three years during two seasons to evaluate their efficiency in Seri industry of tropical region. Out of thirty five ten breeds had been short listed based on ranking for individual trait. Three breeds out of those ten selected breeds viz. SK6, NB18 and B.Con.4 ranked top in respect of overall performance. The ten breeds based on average Evaluation Index value > 50 considering twelve economically important traits are the resource material for future course of breeding program in West Bengal.

• 대한수의학회지
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• 제52권4호
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• pp.231-238
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• 2012

The expression profiles of inflammatory cytokines viz. interleukins (IL)-6, IL-8, IL-12, granulocyte macrophage-colony stimulating factor, interferon-${\gamma}$ and tumor necrosis factor-${\alpha}$ in response to subclinical mastitis in indigenous cattle breed Kankrej (n = 6), Gir (Bos indicus) (n = 12) and crossbred (Bos taurus${\times}$Bos indicus) (n = 7) were investigated using quantitative real time PCR. Significant correlation (p < 0.05) was observed between total bacterial load and somatic cell count (SCC) in all three breeds of cattle. All the cytokines were observed to be up-regulated compared to cows with healthy quarters, however, level of their expression varied among three breeds of cattle. In Kankrej most cytokines were found to be transcribed to higher levels than in other two breeds; the milk had higher load of bacteria but not so high SCC, implying that Kankrej has a higher inherent resistance against mastitis. The results of present study indicated that mammary glands of crossbred cattle are more sensitive to bacterial infection than indigenous breed of cattle as they elicit immune response at lower bacterial load and result into higher SCC. Research on identification of factors responsible for differentially expressed cytokines profiles and use of cytokines as immunomodulatory tools can pave way for formulating control strategies against bovine mastitis.

• 한국축산식품학회:학술대회논문집
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• 한국축산식품학회 2006년도 정기총회 및 제37차 춘계 국제학술발표대회
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• pp.179-183
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• 2006

Denaturing high-performance liquid chromatography(DHPLC) is used in a wide variety of genetic applications and is an efficient method for detection of mutations involving one or a few nucleotides. We developed a high-throughput DHPLC method for identifying polymorphisms in the MC1R gene that are characteristic of Hanwoo cattle. We compared 10 tissue samples from Hanwoo cattle, 10 samples from Holstein cattle and 10 samples from Hanwoox Holstein crossbred cattle to determine whether DHPLC analysis can be used to distinguish between these genotypes. Samples obtained from Hanwoo cattle had a unique profile of peaks that could be used as a molecular fingerprint for this breed. We also analyzed two hundred samples in a trial in which we were blinded to the genotype of the samples and correctly identified the breed-of-origin of 594 out of 600 sequence variations(99%).

• 한국동물생명공학회지
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• 제36권3호
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• pp.154-161
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• 2021

A number of Korean Chicken breeds were registered in Domestic Animal Diversity Information System (DAD-IS, http://dad.fao.org/) of the Food and Agriculture Organization (FAO). Evaluation of genetic diversity and relationship of local breeds is an important factor towards the identification of unique and valuable genetic resources. Therefore, this study aimed to analysis the genetic diversity and relationship of 22 Korean Chicken breeds using 12 microsatellite (MS) markers. The mean number of alleles for each variety was 5.52, ranging from a 3.75 (Leghorn F; NF) to a 7.0 (Ross). The most diverse breed was the Hanhyup3 (HCC), which had the highest expected heterozygosity (H_Exp) (0.754) and polymorphic information content (PIC) (0.711). The NF was the least diverse population, having the lowest H_Exp (0.467) and PIC (0.413). As a result of the principal coordinates analysis (PCoA) and factorial correspondence analysis (FCA) confirmed that Hy-line Brown (HL) and Lohmann Brown (LO) are very close to each other and that Leghorn and Rhode Island Red (RIR) are clearly distinguished from other groups. Thus, the reliability and power of identification using 12 types of MS markers were improved, and the genetic diversity and probability of individual discrimination were confirmed through statistical analysis. This study is expected to be used as basic data for the identification of Korean chicken breeds, and our results indicated that these multiplex PCR marker sets will have considerable applications in population genetic structure analysis.

• 한국축산식품학회지
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• 제27권2호
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• pp.150-156
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• 2007

본 연구는 서로 다른 상염색체에 위치하고 있는 초위성체 유전 표지를 활용한 한국재래돼지 집단의 개체 식별시스템 설정을 위해 수행되었다. 공시재료는 4품종에서 총 446두가 사용되었으며 13종의 좌위에 대한 개체별 유전자형을 분석하였다. 이들 13종에서 출현된 이형접합도는 0.286-0.686였으며 marker 다형성 정보량은 0.399-0.796로 나타났다. 한국 재래돼지 집단에서 나타난 대립 유전자 발현 특성은 다른 대조 품종 집단과 매우 상이한 결과를 나타냈다. S0228좌위는 전체 8종의 대립유전자가 나타난 가운데 다른 3품종에서는 발현이 되지 않은 235 대립유전자가 한국 재래돼지 집단에서만 발현이 되었다. 5종의 초위성체 유전 표지를 활용할 경우 누적 개체 식별력은 99.999%를 나타냈으며 두 마리의 서로 다른 개체가 서로 같은 유전자형을 가질 짝확률은 $0.36{\times}10^{-9}$으로 추정되었다. 따라서 10종의 선정된 유전 표지는 한국재래돼지 집단에서 적정 신뢰도를 제공할 수 있는 개체 식별 시스템을 설정할 수 있을 것으로 생각된다.