• 대한방사선치료학회지
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• 제27권2호
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• pp.131-143
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• 2015

목 적 : MRI를 기반으로 한 CT fusion plan과 MRI only based plan을 비교함으로써 MRI only plan의 유용성을 평가하고, 나아가 Simulation과 치료계획을 포함한 방사선치료의 전 과정을 CT 촬영 없이 MRI 영상으로 구성하여 실시간 MR-IGRT를 구현하고자 한다. 대상 및 방법 : 본원에서 사용중인 BBB CT (Brilliance Big Bore CT, 16slice, Philips)와 Viewray MRIdian System (Viewray, USA)으로 환자 11명(Head and Neck 1명, Breast 5명, Lung 1명, Liver 3명, Prostate 1명)을 대상으로 CT & MR simulation 및 치료계획을 세웠다. Head and Neck, Breast, Prostate환자는 자유호흡(Free Breathing) 상태로, Lung과 Liver 환자는 흡기 호흡중지(Inhalation Breathing Holdng)상태로 Simulation을 진행하였다. Viewray의 Bore 크기 및 Coil 위치를 고려하여 환자 자세 및 고정기구를 동일한 조건을 유지하여 CT simulation을 시행하였다. Viewray MRIdian 시스템을 이용하여 MR 영상을 기반으로 한 CT fusion plan과 CT fusion 없이 [ICRU 46]에서 권고하는 폐, 공기, 뼈의 전자밀도를 입력한 동일한 조건의 MR only plan을 세웠다. Head and Neck, Breast, Prostate는 IMRT, Lung과 Liver 는 Gating치료계획을 세웠고, 치료계획의 평가는 PTV의 균질성 지표 (Homogeneity Index, HI)와 일치성 지표 (Conformity Index, CI), 그리고 각 PTV와 OAR의 DVH와 선량차이를 각각 비교하였다. 결 과 : 두 치료계획간 치료부위별 PTV에 대한 HI 값의 차이는 Head and Neck, Breast, Lung, Liver, Prostate 부위별로 각각 0.089, 0.26, 0.67, 0.2, 0.4%로 나타났으며, CI 값의 차이는 부위별로 각각 0.043, 0.84, 0.68, 0.46, 0.3%로 두가지 평가 값 모두 Head and Neck 부위가 가장 작은 차이를 나타냈다. PTV에 대한 평균선량 차이는 치료부위별로 각각 0.07, 0.29, 0.18, 0.3, 0.18 Gy로 나타났다. 이를 백분율로 나타냈을 때 0.06, 0.7, 0.29, 0.69, 0.44%으로 모두 1% 이하의 차이를 보였다. 두경부암의 각 OAR은 전체적으로 0.01~0.12 Gy의 평균선량 차이를 보였으며, 유방암은 0.04~0.06 Gy, 폐암에서는 0.01~0.21 Gy, 간암은 0.06~0.27 Gy, 전립선암은 0.02~0.23 Gy의 평균선량 차이를 나타냈다. 결 론 : MR 영상을 이용한 치료계획은 연부조직에서 탁월한 대조도를 나타낼 뿐만 아니라 CT fusion한 MR 치료계획과 비교했을 때 PTV의 HI, CI, 선량차이 모두 1%미만의 차이를 보였으며, OAR의 경우 비균질한 조직이 많은 부위일수록 최대 0.89 Gy 선량차이를 보였다. 이결과를 토대로 두경부암, 부분적 유방암이나 전립선암등 비균질도 차이가 적은 부위에는 CT촬영 없이 MR 영상만을 이용한 방사선치료의 가능성을 확인 할 수 있었다.

• 임산에너지
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• 제18권2호
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• pp.92-99
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• 1999

임분밀도에 따른 소나무림의 biomass생산량, 밀도변화에 따른 임목의 부위별 biomass배분특성 및 교목, 관목, 초본 등 수직층위별 biomass의 분포특성을 파악하고자 중국 백두산 동북부 지역 소나무 천연림에서 연구를 수행하였다. 임분의 밀도를 5등급으로 나누어 각각 교목층, 관목층, 초본층별로 biomass량을 추정하였다. 교목층에서 부위별로 교목층 소나무 biomass량 추정식을 유도한 결과 줄기,수피 및 지상부 전체 biomass량의 경우, logW=a＋blog(D2H)＋c(D2H)식의 적합도가 높게 나타났고 가지, 침엽의 biomass량 및 엽면적의 경우, logW=a＋blogD＋cDtlr의 적합도가 높게 나타났다. 밀도가 증가함에 따라 교목층의 biomass량은 증가하였고 관목과 초본층의 biomass량은 감소하는 추이를 나타내었다. 소나무 천연림내 교목층 임목의 부위별 순생산량은 밀도가 증가함에 따라 모두 증가하였다. 침엽이 지상부 전체 biomass 순생산량 중 차지하는 비율은 밀도가 증가함에 따라 감소함으로써 침엽의 교목층 지상부 biomass량에 대한 생산성은 증가하였다. 밀도가 다른 소나무천연림에서 부위별 순생산량은 모두 줄기>침엽>가지>수피 순이었다.

• Journal of Ecology and Environment
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• 제37권4호
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• pp.177-184
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• 2014

This study was conducted to develop allometric equations and to determine the stem density and biomass expansion factor (BEF) for the estimation of the aboveground and belowground biomass of Cryptomeria japonica in Jeju Island, Korea. A total of 18 trees were harvested from the 40-year-old C. japonica stands in Hannam experimental forest, Jeju Island. The mean biomass of the C. japonica was $50.4Mg\;ha^{-1}$ in stem wood, $23.1Mg\;ha^{-1}$ in root, $9.6Mg\;ha^{-1}$ in branch, $4.6Mg\;ha^{-1}$ in needle and $4.3Mg\;ha^{-1}$ in stem bark. The diameter at breast height (DBH) was selected as independent variable for the development of allometric equations. To evaluate the performance of these equations, coefficient of determination ($R^2$) and root mean square error (RMSE) were used and results of the evaluation showed that $R^2$ ranged from 71% (root biomass equation) to 96% (aboveground biomass equation) and the RMSE ranged from 0.10 (aboveground biomass equation) to 0.33 (root biomass equation). The mean stem density of C. japonica was $0.37g\;cm^{-3}$ and the mean aboveground BEF was $1.28g\;g^{-1}$. Furthermore, the ratio of the root biomass to aboveground biomass was 0.32.

• Journal of Ecology and Environment
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• 제31권4호
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• pp.333-339
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• 2008

We investigated death rates, growth rates and recruitment of culms in two neighboring bamboo (Phyllostachys bamboosoides) stands nested in by two different bird species to analyze stand structure and to design conservation strategies. A third bamboo grove not used by birds, the Taewha stand, was included as a control stand. The bamboo stand occupied by birds in the family Ardeidae (the Ardeidae stand) had an approximately 1.5 times higher culm density than the stand occupied by birds in the family Corvidae (the Corvidae stand). The crude death rate and the number of newly emerged shoots were also higher in the Ardeidae stand than the Corvidae stand. The death rate for bamboo in the Ardeidae stand was not dependent on diameter at breast height (DBH) and was almost 40% for culms of all sizes, whereas most dead culms in the Corvidae stand were < 4 cm DBH. Consequently, we conclude that in the Ardeidae stand, density-independent causes of death are operating, while density-dependent factor are more important in the Corvidae site. The results of soil analysis in these stands suggest that the density-independent death pattern observed in the Ardeidae stand may be due to soil acidification resulting from wastes produced by the birds during breeding. On the other hand, the culm distribution and death patterns in the Corvidae stand suggest that the stand characteristics were not affected by the nesting birds. These results suggest that different conservation strategies must be applied to conserve bamboo groves used by ardeids and corvids for nesting.

• Journal of Ginseng Research
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• 제36권2호
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• pp.169-175
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• 2012

Ginseng has been used as a traditional medicine for treatment of many diseases and for general health maintenance in people of all ages. Ginseng is also used to ameliorate menopausal systems. We investigated the estrogenic activity of Korean red ginseng (KRG) in a transient transfection system, using estrogen receptor (ER) and estrogen-responsive luciferase plasmids in MCF-7 cells. The extract activated both ER${\alpha}$ and ER${\beta}$. KRG modulated the mRNA levels of estrogen-responsive genes such as pS2 and ESR1 and decreased the protein level of ER${\alpha}$. In order to examine in vivo estrogenic activity of KRG, sixteen female Sprague-Dawley rats separated into four groups were studied for nine weeks: non-ovariectomized (OVX) rats treated with olive oil, OVX rats treated with olive oil, OVX rats treated with 17-${\beta}$-estradiol (E2) in olive oil, and OVX rats treated with KRG extract in olive oil. The experiments were repeated for three times and the data of twelve rats were combined. Body weight of OVX rats was greater than that of sham-operated control rats and was decreased by E2 treatment. Uterine weight increased after E2 treatment compared to OVX rats. However, no difference in body or uterine weight was observed with KRG intake. KRG induced reductions in total cholesterol, low density lipoprotein cholesterol/total cholesterol, high density lipoprotein cholesterol/total cholesterol, and low density lipoprotein cholesterol/high density lipoprotein cholesterol, but not to the same degree as did E2 intake. These results show that KRG does contain estrogenic activity as manifested by in vitro study but the activity is not strong enough to elicit physiological responses.

• 한국농림기상학회지
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• 제10권3호
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• pp.102-106
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• 2008

상수리나무임분을 대상으로 산림지역에서 쉽게 측정이 가능한 임목밀도, 평균 흉고직경, 평균 수고, 흉고단면적합 등의 임분특성과 낙엽낙지량과의 관계를 조사한 결과 임분 특성은 꽃과 기타물질을 제외하고 낙엽낙지 구성성분과 유의적인 상관관계가 없는 것으로 나타났다. 약 20개월의 조사기간동안 낙엽낙지 구성성분 중 가장 높은 비율을 차지하는 낙엽량의 경우 흉고단면적합과 상관관계가 없는 것으로 나타났고, 임목밀도도 낙엽량에 유의적인 영향을 미치지 않는 것으로 나타났다. 평균 흉고직경의 경우 임목밀도의 영향을 많이 받기때문에 임목밀도의 결과와 유사하며, 평균 수고와 낙엽량의 경우도 유의적인 상관관계가 없는 것으로 나타났다. 이는 성숙한 상수리나무임분의 경우 임관이 울폐되어, 여러 가지 임분특성이 낙엽낙지량에 미치는 영향이 크지 않기 때문으로 그 결과 낙엽낙지량의 년 변화도 유의적인 차이가 없는 것으로 나타났다.

• 한국산림과학회지
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• 제96권5호
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• pp.602-608
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• 2007

Forest plantations become important strategy not merely for the financial aspect, but for carbon sequestration and ecosystem stability. Forest plantations increase the density of the forest biomass, which reduce the increase in atmospheric carbon dioxide. Biomass density is also a useful variable for comparing structural and functional attributes of forest ecosystems across a wide range of environmental conditions. In this study, carbon sequestration of teak (Tectona grandis Linn. f.) in the individual tree and plantation levels estimation was carried out Site-specific allometric equation for the estimation of teak tree biomass was developed based on the direct measurement of fifteen (15) harvested trees in the Oak-twin Township of the Bago Yoma Region, Myanmar. A regression equation of the diameter at breast height (DBH) and the aboveground biomass (carbon content) was constructed to estimate the carbon storage level of plantations, which averaged 79 ton/ha. The average carbon accumulation in the soil (up to 30 cm in depth) was estimated 38.89 ton/ha, The highest mean annual increment (MAI) of total carbon was found in the 6-yr-old teak plantation (12.10 ton/ha/yr) whereas the lowest MAI was in the 26-yr-old teak plantation (4.31 ton/ha/yr).

• Asian-Australasian Journal of Animal Sciences
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• 제16권10호
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• pp.1501-1509
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• 2003

The simultaneous addition of xylanase (5,600 EXU/kg) and phytase (500 FTU/kg) feed enzymes to wheat-based broiler diets was investigated. Starter, grower and finisher diets, with three tiers of nutrient specifications, were fed to 1,440 broiler chicks kept on deep litter from 1-42 days post-hatch, without and with xylanase plus phytase, to determine the effects of diet type and enzyme supplementation on growth performance. The nutrient specifications of type A diets were standard; energy density and protein/amino acid levels were reduced on a least-cost basis to formulate type B diets and further reduced to type C diets. Phosphorus (P) and calcium (Ca) levels were adjusted in supplemented diets. From 1-42 days post-hatch, diet type significantly influenced growth performance. Birds on type C diets had lower growth rates (2,429 vs. 2,631 g/bird; p<0.001), higher feed intakes (4,753 vs. 4,534 g/bird; p<0.005) and less efficient feed conversion (1.96 vs. 1.72; p<0.001) than birds offered type A diets. Enzyme supplementation increased growth rates by 3.2% (2,580 vs. 2,501 g/bird; p<0.005) and improved feed efficiency by 2.7% (1.80 vs. 1.85; p<0.05) over the entire feeding period. There were no interactions between diet type and enzyme supplementation. At 21 days, 5 out of 30 birds per pen were transferred to cages to ascertain treatment effect on apparent metabolisable energy (AME) and nitrogen (N) retention. Xylanase plus phytase enhanced AME (13.48 to 13.91 MJ/kg DM; p<0.001) and N retention (56.3 to 59.7%; p<0.005). Carcass and breast weights of the caged birds were determined following commercial processing. Diet type significantly influenced breast weight, carcass weight and yield. Birds offered Type A diets, in comparison to Type C diets, supported heavier breast (467 vs. 424 g; p<0.001) and carcass weights (1,868 vs. 1,699 g; p<0.001) with superior carcass yields (71.8 vs. 70.6%; p<0.005). Enzyme addition increased carcass weight by 3.9% (1,752 vs. 1,821 g; p<0.005) and breast weight by 5.8% (431 vs. 456 g; p<0.01) without influencing yields. Feed ingredient costs per kg live weight gain and per kg carcass weight indicated that enzyme addition was economically feasible, where supplementation of Type A diets generated the most effective results. Importantly, soluble and total non-starch polysaccharide and phytate contents of the wheat used were typical by local standards. This study confirms the potential of supplementing wheat-based broiler diets with xylanase plus phytase but further investigations are required to define the most appropriate inclusion rates and dietary nutrient specifications in this context.

• 한국가금학회지
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• 제37권3호
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• pp.207-213
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• 2010

본 시험은 목초액이 토종닭 한협 3호의 생산성, 유해 가스 발생량, 혈액 성상 그리고 가슴육의 지방산 함량에 미치는 영향을 평가하기 위하여 토종닭 사료에 수준별로 첨가하여 70일 동안 급여하였다. 목초액은 0, 0.1, 0.2% 수준으로 첨가 급여하였고, 처리구당 5반복, 반복당 16수씩 총 240수를 시험에 이용하였다. 시험 결과, 목초액은 생산성에 있어서 큰 영향을 미치지 못하였으나, 암모니아 가스 발생량은 대조구에 비하여 유의하게 감소시켰다(p<0.05). 혈중 total cholesterol과 HDL-cholesterol 농도에 있어서는 목초액 처리구가 대조구에 비하여 유의하게 증가한 반면 triglyceride의 농도는 감소하였다(p<0.05). 가슴육의 포화지방산인 myristic acid와 stearic acid는 목초액을 급여함에 따라 대조구에 비하여 유의적으로 감소하였으나, 불포화지방산인 oleic acid와 linolenic acid는 유의적으로 증가하였다(P<0.05). 따라서 본 연구 결과, 목초액은 유해 가스 저감 효과가 있으며, 토종닭의 혈중 지질 수준을 개선시키고, 가슴육의 포화지방산 함량을 감소시키고 단쇄 불포화지방산의 함량은 증가시키는 것으로 판단된다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권24호
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• pp.10659-10663
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• 2015

Background: Rapamycin is an effective anti-angiogenic drug. However, the mode of its action remains unclear. Therefore, in this study, we aimed to elucidate the antitumor mechanism of rapamycin, hypothetically via apoptotic promotion, using MCF-7 breast cancer cells. Materials and Methods: MCF-7 cells were plated at a density of $1{\times}10^5$ cells/well in 6-well plates. After 24h, cells were treated with a series of concentrations of rapamycin while only adding DMEM medium with PEG for the control regiment and grown at $37^{\circ}C$, 5% $CO_2$ and 95% air for 72h. Trypan blue was used to determine the cell viability and proliferation. Untreated and rapamycin-treated MCF-7 cells were also examined for morphological changes with an inverted-phase contrast microscope. Alteration in cell morphology was ascertained, along with a stage in the cell cycle and proliferation. In addition, cytotoxicity testing was performed using normal mouse breast mammary pads. Results: Our results clearly showed that rapamycin exhibited inhibitory activity on MCF-7 cell lines. The $IC_{50}$ value of rapamycin on the MCF-7 cells was determined as $0.4{\mu}g/ml$ (p<0.05). Direct observation by inverted microscopy demonstrated that the MCF-7 cells treated with rapamycin showed characteristic features of apoptosis including cell shrinkage, vascularization and autophagy. Cells underwent early apoptosis up to 24% after 72h. Analysis of the cell cycle showed an increase in the G0G1 phase cell population and a corresponding decrease in the S and G2M phase populations, from 81.5% to 91.3% and 17.3% to 7.9%, respectively. Conclusions: This study demonstrated that rapamycin may potentially act as an anti-cancer agent via the inhibition of growth with some morphological changes of the MCF-7 cancer cells, arrest cell cycle progression at G0/G1 phase and induction of apoptosis in late stage of apoptosis. Further studies are needed to further characterize the mode of action of rapamycin as an anti-cancer agent.