• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.45-45
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• 2018

We had already reported the successful germination for green pods of purple lady's slipper orchid (Cypripedium macranthos Sw.). The green pod methods is to take immature seeds in green capsules, sterilize the capsule, and take out the sterile seeds. This method, however, needs very critical time of harvest. The critical time of seed harvest changes depending upon the species, condition of the specimen, and climatic influence, and the right time lies between 5 and 12 weeks after fertilization. In this study, the mature seeds were collected after 120-130 days with hand-polination of lady's slipper orchids. Mature seeds are usually dormant and it has to be overcome, either with hormone or storing the seeds near freezing for two or three months to break dormancy. The seeds were first surface sterilized with 70% ethanol and then transferred 1% NaOCl for 10-15 minutes, followed by rinses 3 times with sterilized distilled water. The cypripedium seeds consists of an embryo within a seed coat known as a testa. The testa is water repellent and the seed has a large air space between the embryo and testa so the seed tends to float on water. We had resolved the problems with vacuum pump to soak water into the testa before sterilization. The seeds were placed on liquid or agar solidified germination media. Cultures were incubated at $24{\pm}1^{\circ}C$ in dark. The seeds were germinated in 6-8 weeks in liquid suspension culture (germination percentage over 18%); however, the seeds on agar solidified media took more than 5 months to germinate and the germination percentage less than 5%. The most effective media for liquid culture was 1/4 strength Murashige and Skoog (MS) medium with 50 ml/l coconut water ($4brix^{\circ}$) at pH 5.8.

• Journal of Ginseng Research
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• v.43 no.1
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• pp.38-48
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• 2019

Background: Interspecific ginseng hybrid, Panax ginseng ${\times}$ Panax quenquifolius (Pgq) has vigorous growth and produces larger roots than its parents. However, F1 progenies are complete male sterile. Plant tissue culture technology can circumvent the issue and propagate the hybrid. Methods: Murashige and Skoog (MS) medium with different concentrations (0, 2, 4, and 6 mg/L) of 2,4-dichlorophenoxyacetic acid (2,4-D) was used for callus induction and somatic embryogenesis (SE). The embryos, after culturing on $GA_3$ supplemented medium, were transferred to hormone free 1/2 Schenk and Hildebrandt (SH) medium. The developed taproots with dormant buds were treated with $GA_3$ to break the bud dormancy, and transferred to soil. Hybrid Pgq plants were verified by random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analyses and by LC-IT-TOF-MS. Results: We conducted a comparative study of somatic embryogenesis (SE) in Pgq and its parents, and attempted to establish the soil transfer of in vitro propagated Pgq tap roots. The Pgq explants showed higher rate of embryogenesis (~56% at 2 mg/L 2,4-D concentration) as well as higher number of embryos per explants (~7 at the same 2,4-D concentration) compared to its either parents. The germinated embryos, after culturing on $GA_3$ supplemented medium, were transferred to hormone free 1/2 SH medium to support the continued growth and kept until nutrient depletion induced senescence (NuDIS) of leaf defoliation occurred (4 months). By that time, thickened tap roots with well-developed lateral roots and dormant buds were obtained. All Pgq tap roots pretreated with 20 mg/L $GA_3$ for at least a week produced new shoots after soil transfer. We selected the discriminatory RAPD and ISSR markers to find the interspecific ginseng hybrid among its parents. The $F_1$ hybrid (Pgq) contained species specific 2 ginsenosides (ginsenoside Rf in P. ginseng and pseudoginsenosides $F_{11}$ in P. quinquefolius), and higher amount of other ginsenosides than its parents. Conclusion: Micropropagation of interspecific hybrid ginseng can give an opportunity for continuous production of plants.

• Korean Journal of Environmental Agriculture
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• v.33 no.4
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• pp.358-363
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• 2014

BACKGROUND: Many strawberry growers are utilizing daylength extension by using incandescent bulb or fluorescent lamp to break dormancy of strawberry induced by low temperature and short day conditions. Conventional incandescent bulb and fluorescent lamp consume a lot of electricity and have short longevity. Red light known for most efficient wavelength for daylength extension light of short-day plant and long-day plant. This study was conducted to verify the effects of red light to enhance growth and to increase production of strawberry (Fragaria ${\times}$ ananassa Duch. cvs. "Seolhyang") METHODS AND RESULTS: Three red light (660nm) of 0.70, 0.87, and $1.05{\mu}mol/m^2/s$ (PAR) and conventional incandescent bulb of 40 Lux were treated respectively under the pot experiment. All treatment irradiated from 18:00 to 24:00 for 6 hours. Red light treatment tend to increase leaf stem number, flower stem number, weight of flower stem, crown weight, root weight, and leaf area of strawberry then incandescent bulb treatment. In field experiment, red light of $0.7{\mu}mol/m^2/s$ (PAR) and conventional incandescent bulb of 40 Lux were irradiated respectively. Field experiment showed that the leaf number, leaf weight, and crown weight of strawberry increased than those of incandescent bulb control with red LED of $0.7{\mu}mol/m^2/s$ (PAR). Red LED treatment increased the fruit number over 15g than incandescent bulb. Furthermore, red LED treatment decreased fruit number below 15g of strawberry than incandescent bulb treatment. Therefore, We believed that red LED treatment increased marketable fruit number by increment of weight of each fruit. Consequently, marketable fruit number, fruit weight, and fruit production of strawberry were increased than those of incandescent bulb by 5 %, 2.9 %, and 8.5 % respectively, but not showed significantly differences. CONCLUSION: These results presumably due to directly enhanced photosynthesis of strawberry leaves and activated action of Pfr phytochrome form by red light. In conclusion, red LED of 660nm could be used for daylength extension light source to enhance production of strawberry.

• Korean Journal of Medicinal Crop Science
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• v.5 no.3
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• pp.169-176
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• 1997

Gibberellin (GA) has been widely used to break seed dormancy for better stand establishment. The experiment was done to clarify the effect of $GA_3$ (concentration; period) and light quality (red; white; dark) after sowing on seed germination and radicle elongation of Campanulaceae (Platycodon grandiflorum; Codonopsis lanceolata; C. pilosula) to get an information on their field emergence. The germination test was carried out with 12 hours irradiation for 9 days after priming treatment. In the darkness, the mean germination rate of all the species was decreased in the order to P. grandiflorum, C. lanceolata, C. pilosula. Their mean germination rates and radicle lengths were increased with increased concentration to 0.1mM of $GA_3$. Earlier germination rate was higher but later one was less in 4-day $GA_3$ treatment than in 1- or 2-day $GA_3$ treatment. Light treatment. especially red light given after $GA_3$ treatment. eliminated the $GA_3$ treatment effect. Red light done after $GA_3$ treatment nearly blocked the germination of P. grandiflorum and C. pilosula but delayed that of C. pilosula compared to the other light quality treatments having the similar rate. In addition. the radicle elongation of all three species affected by light quality treatment showed the same result as the germination rate.