• The Korean Journal of Physiology and Pharmacology
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• 제21권1호
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• pp.125-131
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• 2017

Status epilepticus is the most common serious neurological condition triggered by abnormal electrical activity, leading to severe and widespread cell loss in the brain. Lithium has been one of the main drugs used for the treatment of bipolar disorder for decades, and its anticonvulsant and neuroprotective properties have been described in several neurological disease models. However, the therapeutic mechanisms underlying lithium's actions remain poorly understood. The muscarinic receptor agonist pilocarpine is used to induce status epilepticus, which is followed by hippocampal damage. The present study was designed to investigate the effects of lithium post-treatment on seizure susceptibility and hippocampal neuropathological changes following pilocarpine-induced status epilepticus. Status epilepticus was induced by administration of pilocarpine hydrochloride (320 mg/kg, i.p.) in C57BL/6 mice at 8 weeks of age. Lithium (80 mg/kg, i.p.) was administered 15 minutes after the pilocarpine injection. After the lithium injection, status epilepticus onset time and mortality were recorded. Lithium significantly delayed the onset time of status epilepticus and reduced mortality compared to the vehicle-treated group. Moreover, lithium effectively blocked pilocarpine-induced neuronal death in the hippocampus as estimated by cresyl violet and Fluoro-Jade B staining. However, lithium did not reduce glial activation following pilocarpine-induced status epilepticus. These results suggest that lithium has a neuroprotective effect and would be useful in the treatment of neurological disorders, in particular status epilepticus.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.22-22
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• 2018

Based on medicinal plant references from Vietnam, 1884 flowering plant species (194 families, 956 genera) can be used to treat 30 diseases or have 4 valuable uses such as Tranquillizer, Detoxify, Galactopoietic and Diuretic. 23 species (15 families, 18 genera) for Tranquillizer, 94 species (50 families, 79 genera) for Vaginitis, 18 species (13 families, 15 genera) for Paralytic, 6 species (6 families, 6 genera) for Obese, 60 species (28 families, 50 genera) for Flu, 63 species (37 families, 56 genera) for Eyesore, 96 species (45 families, 77 genera) for Toothache, 97 species, (50 families, 86 genera) for Detoxify, 18 species (18 families, 18 genera) for Syphilis, 80 species (50 families, 71 genera) for Asthma, 17 species (8 families, 11 genera) for HIV AIDS, 56 species (41 families, 54 genera) for Gonorrhoea, 378 species (108 families, 56 genera) for Dysentery, 31 species (22 families, 29 genera) for Galactopoietic, 131 species (69 families, 116 genera) for Diuretic, 11 species (9 families, 9 genera) for Mump, 737 species (129 families, 626 genera) for "Snack bite", 23 species (18 families, 22 genera) for Urolithiasis, 134 species (56 families, 122 genera) for Malaria, 462 species (113 families, 323 genera) for Rheumatism, 55 species (34 families, 49 genera) for Diabetes, 87 species (42 families, 70 genera) for Heart and blood pressure diseases, 70 species (46 families, 63 genera) for Haemorrhoids, 21 species (16 families, 20 genera) for Cancer, 42 species (27 families, 38 genera) for Gastritis, 154 species (66 families, 129 genera) for Hepatitis, 5 species (5 families, 5 genera) for Keratitis, 81 species (42 families, 75 genera) for Sore throat, 11 families (9 families, 11 genera) for Encephalitis, 72 species (41 families, 66 genera) for Nephritis, 10 species (6 families, 8 genera) for Sinusitis, 22 species (17 families, 20 genera) for Sterile, 19 species (14 families, 17 genera) for Cirrhosis, 3 species (3 families, 3 genera) for Brain hemorrhage. Each species can be used to treat some diseases. The information of species can be used to orient researches fast and effectively.

• Asian Pacific Journal of Cancer Prevention
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• 제14권1호
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• pp.309-314
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• 2013

Aim: Brain tumors almost universally have fatal outcomes; new therapeutics are desperately needed and will only come from improved understandins of glioma biology. Methods: Exosomes are endosomally derived 30~100 nm membranous vesicles released from many cell types. Examples from GL26 cells were here purified using density gradient ultracentrifugation and monitored for effects on GL26 tumor growth in C57BL/6j mice (H-2b). Lactate dehydrogenase release assays were used to detect the cytotoxic activity of CD8+T and NK cells. Percentages of immune cells producing intracellular cytokines were analyzed by FACS. Results: In this study, exosomes from murine-derived GL26 cells significantly promoted in vivo tumor growth in GL26-bearing B6 mice. Then we further analyzed the effects of the GL26 cells-derived exosomes on immune cells including CD8+T, CD4+T and NK cells. Inhibition of CD8+T cell cytotoxic activity was demonstrated by CD8+T cell depletion assays in vivo and LDH release assays in vitro. The treatment of mice with exosomes also led to a reduction in the percentages of CD8+T cells in splenocytes as determined by FACS analysis. Key features of CD8+T cell activity were inhibited, including release of IFN-gamma and granzyme B. There were no effects of exosomes on CD4+T cells and NK cells. Conclusion: Based on our data, for the first time we demonstrated that exosomes from murine derived GL26 cells promote the tumor growth by inhibition of CD8+T cells in vivo and thus may be a potential therapeutic target.

• 한국전자통신학회논문지
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• 제6권6호
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• pp.839-847
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• 2011

원자력발전소의 계측제어계통은 제어, 감시기능을 수행하여 안전운전을 위한 두뇌 역할을 하는 핵심적인 분야이다. 최근 계측제어계통은 마이크로프로세서기반의 디지털 기술을 받아들여 디지털화되었다. 그러나 계측제어계통의 디지털시스템은 아날로그 기반 시스템에 비해 사이버위협에 매우 취약하여, 사이버공격에 의해 발전소 안전에 부정적인 영향을 받을 수 있다. 따라서 사이버침해에 대응할 수 있는 사이버 보안 대책이 계측제어계통에 요구된다. 사이버 보안성이 우수한 계통 설계를 위해서는 계측제어계통을 구성하는 자산에 대한 효과적인 자산분석이 요구된다. 본 연구에서는 원자로 계측제어설계의 사이버보안 적합성을 분석하기 위한 전 단계로 계측제어계통의 디지털 자산을 분석하기 위한 방법론을 제안한다. 제안된 디지털자산 분석 방법은 자산식별, 식별된 자산에 대한 평가방법으로 구성된다. 제안된 자산분석방법은 원자력발전소 계측제어계통의 사이버보안을 위한 자산분석에 응용하였다.

• BMB Reports
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• 제49권4호
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• pp.220-225
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• 2016

Cancer cells have different characteristics due to the genetic differences where these unique features may strongly influence the effectiveness of therapeutic interventions. Here, we show that the spontaneous reactivation of extracellular signalregulated kinase (ERK), distinct from conventional ERK activation, represents a potent mechanism for cancer cell survival. We studied ERK1/2 activation in vitro in SW480 colorectal cancer cells. Although ERK signaling tends to be transiently activated, we observed the delayed reactivation of ERK1/2 in epidermal growth factor (EGF)-stimulated SW480 cells. This effect was observed even after EGF withdrawal. While phosphorylated ERK1/2 translocated into the nucleus following its primary activation, it remained in the cytoplasm during late-phase activation. The inhibition of primary ERK1/2 activation or protein trafficking, blocked reactivation and concurrently increased caspase 3 activity. Our results suggest that the biphasic activation of ERK1/2 plays a role in cancer cell survival; thus, regulation of ERK1/2 activation may improve the efficacy of cancer therapies that target ERK signaling.

• Molecular & Cellular Toxicology
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• 제2권2호
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• pp.120-125
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• 2006

Phenytoin is an anti-epileptic. It works by slowing down impulses in the brain that cause seizures. The recent microarray technology enables us to understand possible mechanisms of genes related to compounds which have toxicity in biological system. We have studied that the effect of a compound related to hepatotoxin in vitro system using a rat whole genome microarray. In this study, we have used a rat liver epithelial cell line WB-F344 and phenytoin as a hepatotoxin. WB-F344 was treated with phenytoin for 1 to 24 hours. Total RNA was isolated at times 1, 6 and 24h following treatment of phenytoin, and hybridized to the microarray containing about 22,000 rat genes. After analysis with clustering methods, we have identified a total of 1,455 differentially expressed genes during the time course. Interestingly, about 1,049 genes exhibited differential expression pattern in response to phenytoin in early time. Therefore, the identification of genes associated with phenytoin in early response may give important insights into various toxicogenomic studies in vitro system.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제11권sup1호
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• pp.72-82
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• 2008

Wilson disease (WD) is an autosomal recessive disorder of copper metabolism that results in accumulation of copper primarily in the liver, the brain and the cornea. Mutations in the WD gene, ATP7B cause failure of copper excretion from hepatocyte into bile and a defective synthesis of ceruloplasmin. More than 370 mutations are now recognized, scattering throughout the ATP7B gene. Since WD has protean clinical presentations, awareness of WD in clinical practice is important for the early diagnosis and prevention of accumulated copper toxicity. None of the laboratory parameters alone allows a definite diagnosis of WD. There are numerous pitfalls in the diagnosis of WD. Low serum ceruloplasmin concentrations, increased 24 hour urinary copper excretion, increased hepatic copper concentrations and the presence of Kayser-Fleischer rings in the cornea are major diagnostic points. A combination of any two of these 4 laboratory findings is strong support for a diagnosis of WD. Molecular methods are now being used to aid diagnosis. Molecular genetic testing has confirmed the diagnosis in individuals in whom the diagnosis is not clearly established biochemically and clinically. Siblings should be screened for WD once an index case has been diagnosed. Discrimination of heterozygotes from asymptomatic patients is essential to avoid inappropriate lifelong therapy for heterozygotes. Genetic testing, either by haplotype analysis or by mutation analysis, is the only reliable tool for differentiating heterozygote carriers from affected asymptomatic patients. Currently, genetic testing is of limited value in the primary diagnosis. However, genetic testing will soon play an essential role in diagnosing WD as rapid advancement of biomedical technology will allow more rapid, easier and less expensive mutation detection.

• The Journal of Korean Physical Therapy
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• 제27권1호
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• pp.38-42
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• 2015

Purpose: Accuracy and variability of movement in daily life require synchronization of muscular activities through a specific chronological order of motor performance, which is controlled by higher neural substrates and/or lower motor centers. We attempted to investigate whether transcranial direct current stimulation (tDCS) over primary sensorimotor areas (SM1) could influence movement variability in healthy subjects, using a tapping task. Methods: Twenty six right-handed healthy subjects with no neurological or psychiatric disorders participated in this study. They were randomly and equally assigned to the real tDCS group or sham control group. Direct current with intensity of 1 mA was delivered over their right SM1 for 15 minutes. For estimation of movement variability before and after tDCS, tapping task was measured, and variability was calculated as standard deviation of the inter-tap interval (SD-ITI). Results: At the baseline test, there was no significant difference in SD-ITI between the two groups. In two-way ANOVA with repeated measurement no significant differences were found in a large main effect of group and interaction effect between two main factors (i.e., group factor and time factor (pre-post test)). However, significant findings were observed in a large main effect of the pre-post test. Conclusion: Our findings showed that the anodal tDCS over SM1 for 15 minutes with intensity of 1 mA could enhance consistency of motor execution in a repetitive-simple tapping task. We suggest that tDCS has potential as an adjuvant brain facilitator for improving rhythm and consistency of movement in healthy individuals.

• Asian Pacific Journal of Cancer Prevention
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• 제16권15호
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• pp.6327-6330
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• 2015

Background: Glioblastoma is a highly aggressive and malignant brain tumor. Risk factors are largely unknown however, although several biomarkers have been identified which may support development, angiogenesis and invasion of tumor cells. One of these biomarkers is PAI-1.4G/5G and A-844G are two common polymorphisms in the gene promotor of PAI 1 that may be related to high transcription and expression of this gene. Studies have shown that the prevalence of the 4G and 844G allele is significantly higher in patients with some cancers and genetic disorders. Materials and Methods: We here assessed the association of 4G/5G and A-844G polymorphisms with glioblastoma cancer risk in Iranians in a case-control study. All 71 patients with clinically confirmed and 140 volunteers with no history and symptoms of glioblastoma as control group were screened for 4G/5G and A-844G polymorphisms of PAI-1, using ARMS-PCR. Genotype and allele frequencies of case and control groups were analyzed using the DeFinetti program. Results: Our results showed significant associations between 4G/5G (p=0.01824) and A-844G (p = 0.02012) polymorphisms of the PAI-1 gene with glioblastoma cancer risk in our Iranian population. Conclusions: The results of this study supporting an association of the PAI-1 4G/5G (p=0.01824) and A-844G (p = 0.02012) polymorphisms with increasing glioblastoma cancer risk in Iranian patients.

• Archives of Plastic Surgery
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• 제40권6호
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• pp.687-696
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• 2013

Background Ischemic preconditioning has been shown to improve the outcomes of hypoxic tolerance of the heart, brain, lung, liver, jejunum, skin, and muscle tissues. However, to date, no report of ischemic preconditioning on vascularized bone grafts has been published. Methods Sixteen rabbits were divided into four groups with ischemic times of 2, 6, 14, and 18 hours. Half of the rabbits in each group underwent ischemic preconditioning. The osteomyocutaneous flaps consisted of the tibia bone, from which the overlying muscle and skin were raised. The technique of ischemic preconditioning involved applying a vascular clamp to the pedicle for 3 cycles of 10 minutes each. The rabbits then underwent serial plain radiography and computed tomography imaging on the first, second, fourth, and sixth postoperative weeks. Following this, all of the rabbits were sacrificed and histological examinations were performed. Results The results showed that for clinical analysis of the skin flaps and bone grafts, the preconditioned groups showed better survivability. In the plain radiographs, except for two non-preconditioned rabbits with intraoperative ischemic times of 6 hours, all began to show early callus formation at the fourth week. The computed tomography findings showed more callus formation in the preconditioned groups for all of the ischemic times except for the 18- hour group. The histological findings correlated with the radiological findings. There was no statistical significance in the difference between the two groups. Conclusions In conclusion, ischemic preconditioning improved the survivability of skin flaps and increased callus formation during the healing process of vascularized bone grafts.