• 감성과학
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• 제8권4호
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• pp.423-433
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• 2005

컴퓨터 하드웨어의 발전은 예술과 기술의 결합을 더욱 결속시키는 계기가 된다. 기계간의 상호작용에서 관객이 간섭할 수 있게 되면 그 결과물은 관객이 작품을 완성한다고 한다 기술의 업그레이드가 진화하며 재사용 되는 경우를 '피드백'이라 말할 수 있다. 백남준의 '참여 TV'는 기계가 인간의 행동에 반응하여 시지각 정보를 실시간으로 행위의 정도에 따라 반응하여 결과물을 보여주는 기본 방식을 갖춘 작품이다 댄 그래함의 작품에서 시공의 피드백을 경험할 수 있다. '연속되는 과거로 이루어지는 현재'라는 설치를 통해 관객의 영상이 거울에 피드백되는 상황 속에서 모니터에서는 5초 전 관객이 했던 영상을 재생시키는 장치를 하였다. 빌 비올라의 영상과 음향의 실시간 확대라는 점에서 감성을 자극하는 공명의 피드백을 일으키고 있는 작품처럼 우연의 시간과 관객의 등장을 바탕으로 한 작가의 철학이 읽혀지는 작품으로 승화되어야 하는 것이 현대 미디어 아트가 풀어 나가야 할 근본적인 화두이다. 스텔락은 몸과 기계 그리고 신경과 두뇌가 발생시키는 신호 체계에 많은 관심을 보였다. 미디어인 몸과 터미널 디스플레이 행위자로 결국 맥루한이 말한 몸의 확장으로서의 미디어 자체가 되었다. 제프리 쇼는 '명료한 도시 legible City'에서 관객의 몸이 관여하여 페달의 속도와 핸들의 움직임에 영상이 반응한다. 작품 'RE:MARK'는 에드몽 뀨쇼(Edmond Couchot)의 '바람에 심는다.' 나 백남준의 '참여 TV'에서 보여주었듯이 마이크를 입력장치로 사용하여 인터렉션을 유도하였다. 피아간의 피드백이 없으면 커뮤니케이션도 없다. 실감을 느끼는 관객들은 작품에 감동한다 피드백 현상이 자연스러워야 하는 이유이다. 작가 정신의 숭고한 고유-독창성(Originality)이 피드백의 경로를 투과하면서 자신을 남에게 투영시켜 얻어내는 것이 고유성의 변종이다. 피드백은 단순한 작품의 일부가 아니라 작품을 이루는 뼈대이다 기술의 과시만으로는 예술의 행위가 될 수 없다. 작가의 예술성이 관객의 감성에 피드백 되도록 노력해야 한다 그러기 위해서는 예정된 피드백이란 느낌을 관객이 갖지 않도록 하여야 한다. 인터렉티브 미디어 아트는 초기의 형태에서 벗어나 새로운 집적된 피드백 기술로 전환하여야 할 시기가 온 것이다.

• 감성과학
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• 제20권1호
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• pp.49-56
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• 2017

본 연구의 목적은 가상현실 볼링이 정서에 미치는 영향을 연구하기 위함이다. 본 실험에 참여한 16명의 피험자는 볼링 경력 3년, 나이는 26-35세, 평균나이 29.6세의 볼러들로 구성하였다. 피험자들이 볼링을 다섯 게임 하는 동안 전두엽의 뇌파를 측정하였으며, 게임이 끝난 후 곧바로 시각상사척도를 사용해 게임 후의 정서를 측정하였다. 뇌파측정부위는 전전두엽, 전두엽, 외측 전두엽이었다. 자료 분석은 전두엽의 알파파에 대해 일원분산분석을 실시하였다. 종속변수는 알파파워 값이다. 연구결과 스트라이크를 쳤을 때는 시각사상척도 값이 스페어나 스페어 처리를 처리했을 때보다 높게 나타났다. 또한 스페어를 처리했을 때는 스페어 미스보다 시각사상척도 값이 높게 나타났다. 대뇌 반구 비대칭 점수와 관련해선 다음과 같은 결과를 얻었다. 좌측 전두엽의 알파파는 스트라이크와 스페어를 처리했을 때 활성화되는 것으로 나타났다. 한편 스페어 미스를 했을 때는 우측 전두엽의 알파파가 활성화되었다. 결론적으로 이 연구는 경쟁에서 성취 또는 성공을 했을 때 긍정적인 정서가 나타나고, 반대로 실패했을 경우에는 부정적인 정서가 나타난다는 것을 보여주었다. 또한 이 연구는 경기 중에 발생하는 수행의 결과가 선수의 정서에 직접적으로 영향을 미친다는 것을 신경생리학적으로 입증해 주었다.

• 대한유전성대사질환학회지
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• 제5권1호
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• pp.48-56
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• 2005

Purpose: Rett syndrome (RTT) is an X-linked dominant neurodevelopmental disorder affecting 1 per 10,000~15,000 female births worldwide. The disease-causing gene has been identified as MECP2 (methyl-CpG-binding protein). In this study, we carried out diagnostic mutational analysis of MECP2 gene in RTT patients. Methods: We analyzed four exons and putative promoter of MECP2 gene from the peripheral blood of 43 Korean patients with RTT by PCR-RFLP and direct sequencing. Results: Mutations were detected in MECP2 gene about 60.5% of patients. The mutations consisted of 14 different types including 9 missense mutations, 4 nonsense mutations and 1 frameshift mutation. Of these, three mutations (G161E, T311M, P385fsX409) were newly identified and these were determined as disease-causing mutations by PCR-RFLP and direct sequencing analysis. Most of the mutations were located within MBD (42.3%) and TRD (50%). T158M, R270X, and R306C mutations were identified with high frequency. An intronic SNP (IVS3+23C>G) was newly identified in only three of the patients. It may be a disease-related and Korea-specific SNP with RTT. The L100V and A201V have been reported to be unclassified variant and SNP. However, these mutations were not found in more than 100 normal Korean control samples. These base substitutions seem to be the disease-causing mutations in Korean RTT contrary to previous studies. Conclusion: Disease-causing mutations and polymorphisms would be very important for diagnosing of RTT in Korean. The experimental procedure used in this study might be considered for molecular biologic diagnosis used in clinical field.

• Fisheries and Aquatic Sciences
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• 제21권2호
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• pp.6.1-6.10
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• 2018

The intention of this study was to identify the bacterial pathogens infecting Oreochromis niloticus (Nile tilapia) and Clarias gariepinus (African catfish), and to establish the antibiotic susceptibility of fish bacteria in Uganda. A total of 288 fish samples from 40 fish farms (ponds, cages, and tanks) and 8 wild water sites were aseptically collected and bacteria isolated from the head kidney, liver, brain and spleen. The isolates were identified by their morphological characteristics, conventional biochemical tests and Analytical Profile Index test kits. Antibiotic susceptibility of selected bacteria was determined by the Kirby-Bauer disc diffusion method. The following well-known fish pathogens were identified at a farm prevalence of; Aeromonas hydrophila (43.8%), Aeromonas sobria (20.8%), Edwardsiella tarda (8.3%), Flavobacterium spp. (4.2%) and Streptococcus spp. (6.3%). Other bacteria with varying significance as fish pathogens were also identified including Plesiomonas shigelloides (25.0%), Chryseobacterium indoligenes (12.5%), Pseudomonas fluorescens (10.4%), Pseudomonas aeruginosa (4.2%), Pseudomonas stutzeri (2.1%), Vibrio cholerae (10.4%), Proteus spp. (6.3%), Citrobacter spp. (4.2%), Klebsiella spp. (4.2%) Serratia marcescens (4.2%), Burkholderia cepacia (2.1%), Comamonas testosteroni (8.3%) and Ralstonia picketti (2.1%). Aeromonas spp., Edwardsiella tarda and Streptococcus spp. were commonly isolated from diseased fish. Aeromonas spp. (n = 82) and Plesiomonas shigelloides (n = 73) were evaluated for antibiotic susceptibility. All isolates tested were susceptible to at-least ten (10) of the fourteen antibiotics evaluated. High levels of resistance were however expressed by all isolates to penicillin, oxacillin and ampicillin. This observed resistance is most probably intrinsic to those bacteria, suggesting minimal levels of acquired antibiotic resistance in fish bacteria from the study area. To our knowledge, this is the first study to establish the occurrence of several bacteria species infecting fish; and to determine antibiotic susceptibility of fish bacteria in Uganda. The current study provides baseline information for future reference and fish disease management in the country.

• 한국양식학회지
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• 제20권1호
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• pp.65-72
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• 2007

우리나라 주요 담수어종인 미꾸라지(Misgurnus mizolepis)로 부터 apolipoprotein A-I (apoA-I) cDNA를 분리하고 그 구조, 분자 계통 및 발현 특징을 분석하였다. 미꾸라지 apoA-I cDNA는 254개의 아미노산을 암호화하고 있는 762 bp의 ORF를 포함하고 있었으며 아울러 24 bp의 5'UTR 및 293 bp의 3'UTR(종결 코돈 및 poly A tail 제외)를 갖고 있었다. 미꾸라지 apoA-I은 여타 척추동물 apoA-I과의 다중배열 시 염기서열에서는 많은 차이를 나타내었지만 단백질의 구조적 특징은 높은 상동성을 보였고, 또한 척추동물의 apoA-I들과의 분자계통을 분석한 결과, 종래 알려진 분류학적 위치와 비교적 잘 일치하였다. 미꾸라지 apoA-I mRNA는 RT-PCR 분석을 통해 간 및 뇌 조직에서 분석한 다른 조직보다 유의적으로 높게 발현하는 것으로 나타났고, 특히 간에서 가장 높은 발현을 보였다. 수정시부터 부화 후 14일까지 초기 발생 및 치어에서의 apoA-I mRNA 발현을 조사한 결과 수정 8시간째부터 급격한 발현의 증가가 시작되어 이후 지속적으로 높은 발현 수준을 유지하였다.

• Journal of Ginseng Research
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• 제41권2호
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• pp.151-158
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• 2017

Background: Chong-Myung-Tang (CMT) extract is widely used in Korea as a traditional herbal tonic for increasing memory capacity in high-school students and also for numerous body ailments since centuries. The use of CMT to improve the learning capacity has been attributed to various plant constituents, especially black ginseng, in it. Therefore, in this study, we have first investigated whether black ginseng-enriched CMT extracts affected spatial learning using the Morris water maze (MWM) test. Their molecular mechanism of action underlying improvement of learning and memory was examined in vitro. Methods: We used two types of black ginseng-enriched CMT extracts, designated as CM-1 and CM-2, and evaluated their efficacy in the MWM test for spatial learning behavior and their anti-inflammatory effects in BV2 microglial cells. Results: Our results show that both black ginseng-enriched CMT extracts improved the learning behavior in scopolamine-induced impairment in the water maze test. Moreover, these extracts also inhibited nitric oxide production in BV2 cells, with significant suppression of expression of proinflammatory cytokines, especially inducible nitric oxide synthase, cyclooxygenase-2, and $interleukin-1{\beta}$. The protein expression of mitogen-activated protein kinase and nuclear $factor-{\kappa}B$ pathway factors was also diminished by black ginseng-enriched CMT extracts, indicating that it not only improves the memory impairment, but also acts a potent anti-inflammatory agent for neuroinflammatory diseases. Conclusion: Our research for the first time provides the scientific evidence that consumption of black ginseng-enriched CMT extract as a brain tonic improves memory impairment. Thus, our study results can be taken as a reference for future neurobehavioral studies.

• 대한수의학회지
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• 제40권2호
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• pp.197-211
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• 2000

The pineal body have been known to be affected by superior cervical ganglia, and most of its nerve fibers containing peptidergic neurotransmitters have been considered to be originated from this ganglia. To confirm this relationships, some peptidergic neurotransmitters were identified in both of pineal body and superior cervical ganglia of the Korean native goat, which were divided into two group; breeding season and non-breeding season. The localizations of two catecholamine-synthesizing enzymes; tyrosine hydroxylase (TH) and dopamine beta-hydroxylase (DBH), were investigated by immunohistochemistry in the superior cervical ganglia and the pineal body of adult Korean native goats. Substance P (SP), calcitonin gene-related peptide (CGRP), vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY) and galanin (GAL) were also identified in these organs by immunohistochemical and double immunofluorescent methods. In superior cervical ganglia, immunoreactivities for TH and DBH were confirmed in the same ganglion cells. The immunoreactivites for SP, VIP(only in male), NPY and GAL were identified in both of ganglion cell bodies and nerve fibers in the ganglia. CGRP immunoreactivity, however, was observed only in nerve fibers. Most NPY- and VIP-immunoreactive(IR) ganglion cells also contained TH. SP and TH were colocalized in the cell bodies, but not in the nerve fibers. TH immunoreactivity was shown in almost all of ganglion cells in the superior cervical ganglia. The immunoreactivity for NPY had some seasonal variation and was stronger in breeding season than in non-breeding season. In pineal body, lots of TH-IR fibers were observed throughout the parenchyma including the pineal stalk and most of them also contained DBH. SP- and NPY-IR fibers were also immunostained with TH or DBH. But a few SP- and NPY-IR fibers were not colocalized with TH or DBH. Exceptionally, a bipolar neuron-like cell was observed to be immunostained with NPY in the pineal body. A few CGRP and GAL-IR fibers were observed, while VIP-IR fibers were not present. It is concluded that most TH- and DBH-IR fibers as well as the peptidergic immunoreactive fibers of the pineal body might be originated from the superior cervical ganglia. Some peptidergic immunoreactive fibers, however, might be come from other regions of brain. We also suggest that NPY in pineal body plays a important role for pineal function. The seasonal variation of NPY immunoreactivity indicates that the synthesis and use of NPY may be different between in breeding and non-breeding seasons.

• 대한수의학회지
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• 제46권3호
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• pp.215-224
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• 2006

In order to search the availability of the lectin extracted from Korean mistletoe(Viscum album coloratum) as an adjuvant for the avian vaccines, attempts were made to determine toxicity of the lectin in chicks and its immunostimulating activity on the inactivated vaccines against Newcastle disease virus(NDV). For the determination of toxicity, the lectin was injected into the thigh muscle of SPF chicks(Charles River) of 1-week-old and observed hematologically and pathologically. For the determination of immunostimulating effects, lectin-adjuvanted, inactivated NDV vaccines were injected into the thigh muscle of SPF chicks in the same age group. Sera of the chicks were examined for the hemagglutination-inhibition(HI) antibodies induced, their HI titers and reaction to the NDV antigens. The data were further compared with those from aluminum hydroxide [$Al(OH)_3$]-adjuvanted vaccines and vaccines without adjuvant, and the results are as follows. There were no significant changes observed in the values of RBC, WBC, Hb, PCV, MCV, MCH, MCHC, AST, ALT, BUN, creatinine and total proteins in the chicks administered with lectin of 1.1, 2.2 and $22.2{\mu}g/kg$ body weight, which means the lectin has no effects on blood values and functions of liver and kidney. In histopathologic observation, no lesions were observed in the brain, heart, liver, lung, spleen, kidney, thymus and bursa of Fabricius of the chicks administered with lectin of 1.1, 2.2 and $22.2{\mu}g/kg$ body weight. There were inflammatory lesions, such as congestion, hemorrhage, edema, infiltration of macrophages and coagulation necrosis observed in the thigh muscle of chicks administered with lectin of $22.2{\mu}g/kg$ body weight, whereas no changes were observed in 1.1 and $22.2{\mu}g/kg$ lectin administered chicks. In chicks immunized with lectin($4.4{\mu}g/kg$ of body weight)-adjuvanted B1, LaSota and Ulster 2C vaccines, HI titers in reciprocal values for $log_2$ were 1.8-2.2 at 1 week after vaccination, which was similar with those of 1.5-2.9 by $Al(OH)_3$-adjuvanted vaccines. The HI titers by the lectin-adjuvanted vaccines reached to 3.9-5.3 at 4 weeks, whereas those by the $Al(OH)_3$-adjuvanted vaccines were more high as 7.3-9.3. Meanwhile, the immunostimulating effects of the lectin were recognized while compared to the HI titers with 2.4-3.7 in chicks immunized with vaccines without adjuvants at 4 weeks after vaccination. The chicks immunized with lectin-adjuvanted vaccines were enough to resist challenges by Kyojeongwon strain, a very virulent NDV at 4 weeks after vaccination as well as chicks immunized with $Al(OH)_3$-adjuvanted vaccines. The HI titers by the lectin-adjuvanted vaccines reached to high level as 8.7-10.3 as those with 8.2-9.6 by the $Al(OH)_3$-adjuvanted vaccines at 6 weeks after vaccination, which may be the booster effects by the challenge virus. Antibodies specific to the HN and F antigens of NDV were observed in the sera of both chicks immunized with lectin-adjuvanted vaccines and $Al(OH)_3$-adjuvanted vaccines.

• 한국수산과학회지
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• 제34권3호
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• pp.245-253
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• 2001

곰소만 조간대에서 채취한 표층퇴적물에 대하여 입도분석을 수행하여 퇴적물의 조직 특성을 분석하였다. 코어 시료를 이용하여 퇴적물의 수리-역학적 특성을 실험적으로 도출하였으며, 퇴적물의 심도별 역학적 응력 및 과잉간극수압, 그리고 지반의 안정성을 수치해석적으로 분석하여 바지락 양식장 퇴적물의 거동 특성을 규명하였다. 곰소만 조간대내의 표층 퇴적물은 겨울에는 $5 \phi$의 조립 실트가 주를 이루는 단모드형을 나타내고, 여름에는 $5 \phi$의 조립 실트질 퇴적물에 $4 \phi$의 미세사질 퇴적물이 첨가된 경향을 보여준다 바지락 양식장의 퇴적환경은 평균 입도 $4\~5 \phi$범위, 모래함량 $50\%$ 미만, 니질 함량 $5\sim10\%$ 범위를 나타낸다. 퇴적양상은sandy silt이며, 분급도는 $1.0\sim2.0 \phi$를 나타내고, 왜도는 0.1-0.5의 범위에 포함되어 퇴적물의 퇴적 작용이 방해가 될만한 높은 에너지 상태가 지속되지 않는 환경이 바지락 양식에 양호한 것으로 조사되었다. 수치해석적 결과를 토대로 볼 때, 바지락 양식에 양호한 저질환경은 사질과 니질이 혼합되어 절대 투수계수가 $10^{-11}\sim10^{-12}m^2$ 범위인 경우로 사료되며, 수치해석 모델링 견과 니질 피복층의 두께가 4cm를 초과하게되면 바지락 서식에 대한 저질환경은 크게 저하되고 있는 것으로 고찰된다.

• 대한간호학회지
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• 제17권3호
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• pp.204-217
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• 1987

It is generally accepted that the delivery of health care is undergoing many changes specially those related to acute, contagious disease care and to the increase of chronic illnesses which can not be cured but are controlable. The health care practitioner can not be soley responsible for the control of their clients' care. Because the clients will play a vital role in controlling their illnesses, long term participation by both the health care provider and the client is necessary. Since most individuals with hypertension do not experience signs or symptoms, the disease is difficult to detect and even when diagnosed, clients do not comply well with their hypertension regimens. The noncompliant client is at increased risk for compliants involving the heart, brain, kidney and other organs. In an effort to explore methods of increasing patient participation in and adherence to treatment programs for hypertension, the researcher used health contracting to promote self care. The research questions are; 1) Will the health contracting increase compliance in health behavior and reduce the blood pressure\ulcorner 2) If clients comply with their regimens will this reduce their blood pressure\ulcorner The research design utilized in this study was a quasi-experimental design. A purposive sample, was abtained from two churches in the 1. area, consisting of 64 clients with hypertension. The data was collected from the middle of January to the 1st of September 1985. Randomization was only of the two church groups into experimental and control groups. Compliance with health behavior related to the hypertensive regimen, blood pressure and body weight were measured, compared and analyzed. In the experimental group measurements were made 6 times; one month before the education program after education program when health contracting was done and 4 more times once a month for 4 months. In the control group measurements were made 3 times; one month before the education program after the education program, and once 4 months later. There was no health contracting. The data were analyzed by t-test, Pearson correlation and ANOVA according to purpose of the study. The result of this study may be summarized as follows: The result related to the hypothesis on the effect of health contracting are as follows: H$_1$; “The hypothesis that the experimental group, with a health contractual agreement will demonstrate increased compliance levels for health behavior than the control group” was supported(t＝-5.29, df＝62, p＝.000). H$_2$; “The hypothesis that the experimental group, with a health contractual agreement, will demonstrate a greater reduction in blood pressure than the control group” was supported (for systolic blood pressure t＝2.72, df＝62, p＝.009, for diastolic blood pressure t＝1.95, df＝62, p＝.050). H$_3$; The hypothesis that the greater the compliance of the client with health behavior the lower the client's blood pressure will be was partially supported (for systolic pressure r＝-.2981, p＝.008, for diastolic pressure r＝-.1720, p＝.087). From the examination of the results of this study it can be concluded that the interaction between the nurse and the client, contracting to define goals and reinforcing compliant behavior, leads to improved compliance with health care behaviors and thus to an increase in the effectiveness of nursing care. Further consideration need to be given to the inclusion of the concept of health contracting in primary nursing and to further research in this area.