• 대한수의학회지
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• 제32권1호
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• pp.77-82
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• 1992

세포 비병원성 소 설사병 바이러스가 감염된 우태아 폐세포를 이용 바이러스를 순수분리하여 이화학적 성상을 검사하였던 바 다음의 결과를 얻었다. 바이러스의 비중은 $1.090{\sim}1,114gm/cm^3$로 검출되었으며 $1,098gm/cm^3$에서 최대 감염가를 나타내었다. 면역 전자현미경을 이용한 형태학적 분석결과 30~80nm의 바이러스성 입자를 관찰할 수 있었으며 유전인자의 추출 분석결과 병원성 바이러스와의 전기영동상 유전자 규모에 있어서 차이는 인정되지 않았다. 그러나 비세포 병원성 소 설사병 바이러스는 병원성 바이러스에 비하여 세포외 배출이 적었으며 세포내에서 보다 많이 존재하는 것으로 관찰되었다.

• 한국동물위생학회지
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• 제47권2호
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• pp.73-79
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• 2024

Bovine viral diarrhea virus (BVDV) is distributed in cattle worldwide and causes significant economic losses to the livestock industry. Identification and remove of BVDV persistently infected (PI) cattle is very important to control BVDV infection in cattle herd. The objective of this study is to investigate the infection status of BVDV infection in Korean native cattle (Bos taurus coreanae) farms located in Jeonbuk State. From 2021 to 2022, a total of 1,497 samples were collected from 17 cattle farms and tested for BVDV antigen using a commercial ELISA kit. By the first ELISA testing, 24 cattle from six farms were positive for BVDV antigen, showing the farm-level or cattle-level prevalence of 35.3% or 1.6%, respectively. By the second ELISA testing which carried out with the first ELISA-positive samples after three-weeks, 12 cattle (0.8%) from five farms (29.4%) were positive for BVDV antigen, indicating these cattle were PI cattle. Genotypes of BVDV were determined with 12 BVDV-positive samples using a previously described RT-PCR assay and the results showed that 3 (25.0%) and 9 (75.0%) were confirmed to be type 1 and type 2, respectively. These results will be helpful to establish the effective control strategy for BVDV in cattle farms in Jeonbuk State.

• 한국임상수의학회지
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• 제29권5호
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• pp.395-399
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• 2012

2011년 경북 영주의 여러 농가에서 이병율과 치사율이 높은 소 바이러스성 설사 바이러스(BVDV)가 발생하였다. 유전자 분석 결과 두 개의 유전자형 BVDV-1b (n = 21)와 BVDV-2a (n = 7)이 확인되었다. 검사결과, 이 지역의 농가에서는 BVDV-1b 유전자형이 가장 많이 검출되었고, 또한 몇 농가에서 BVDV-2a 유전자형도 검출되었다. 이들 농가에서 발생한 BVDV-1b 감염은 BVDV-2 감염과 유사한 중증의 급성 임상증상을 보여 주었다. 이 결과는 한우 송아지에서 급성이면서 치명적인 BVDV-1b 발생을 보고한다.

• 대한수의학회지
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• 제39권4호
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• pp.743-750
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• 1999

Bovine viral diarrhea viruses (BVDVs) were isolated from cattle with respiratory and diarrhea signs as well as persistently infected cattle. These isolates were analysed serologically to characterize serogroups and to compare serological relationship with reference viruses of type I and II. Most isolates from calf diarrheal cases and persistently infected individuals showed a significant difference in cross-neutralization test with the viruses isolated from nasal discharges showing severe respiratory signs. Serologically most of the commercial vaccine strains could be classified into classical BVDV (type I) such as NADL strain. This serological difference among BVDV isolates suggested the need for new vaccines to protect cattle from both respiratory and enteric BVDV infections in field. The immunogenicity of BVDVs which showed a good propagation capability in MDBK cells and high rates of neutralizing activity (isolate : KD26-1, PHG, B5 and 95002) against all viruses used in this study, was confirmed in guinea pig when treated as single or combined groups.

• 대한수의학회지
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• 제32권3호
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• pp.389-398
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• 1992

Molecular cloning was carried out on the Danish strain of bovine viral diarrhea virus(BVDV) to construct strategy for the diagnostic tools and effective vaccine of BVD afterwards. A recombinant DNA clone(No. 29) was established successfully from cDNA for viral RNA tailed with adenine homopolymer at 3'-end. $^{32}P$-labeled DNA probes of 300~1,800bp fragments, originating from the clone 29, directed specific DNA-RNA hybridization results with BVDV RNA. Recombinant DNA of the clone 29 was about 5,200bp representing 41.6% of the full length of Danish strain's RNA, and restriction sites were recognized for EcoR I, Sst I, Hin d III and Pst I restriction enzymes in the DNA fragment.

• 한국동물위생학회지
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• 제36권1호
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• pp.7-13
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• 2013

Bovine viral diarrhea virus (BVDV) is one of the most important disease viruses in cattle that can cause severe economical losses due to decreased fertility, abortion, diarrhea, and respiratory symptoms. Therefore, this study was aimed to investigate prevalence of BVDV infection (Transiently infection, Persistently infection) in dairy cattle in Gyeongnam southern area, Korea and use this data as the basis for establishing an eradication program and policy. A total of 44 bulk-tank milk samples (farms) collected in milk collecting center were tested for BVDV antibody using an ELISA. As the result, out of a total of 44 bulk-tank milk samples (farms), 38 (86.4%) samples were BVDV antibody positive. Blood samples (17 farms, n=543) were collected from BVDV antibody positive farms in bulk-tank milk, tested for BVDV antigen with ELISA and PCR. BVDV infected farms were 47% (8/17) and BVDV infected head were 2.2% (12/543). Persistently infected cattle (PI) were detected at 6 (35.3%) farms out of 17 farms and a total of 6 (1.1%) out of 543 head of cattle were identified as PI. The seropositive of BVDV antibody at farms and head were 100% (17/17) and 49.45% (91/184), respectively. The seroprevalence of BVDV antibody in PI infected farms (67.35%) much higher than that of BVDV antibody in transiently infected cattle (TI) infected farms (45%) and uninfected farms (34.48%). For eradication of BVDV infection in cattle populations, First of all, we should remove PI and need vaccination.

• 한국동물위생학회지
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• 제42권2호
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• pp.117-120
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• 2019

Bovine viral diarrhea Virus (BVDV) infections cause respiratory, gastrointestinal, and reproductive problems, such as infertility, abortion, stillbirth, and sickly offspring. Many countries have reduced the economic damage through the application of different control programmes, and some have successfully eradicated BVD. Detection and elimination of cattle persistently infected (PI) with BVDV is important for BVD eradication because PI cattle are a main source of BVD transmission. In this study, the prevalence of Korean native cattle persistently infected (PI) with BVDV was investigated and determined in 49 farms with 3,050 cattle. The all samples were collected by ear notch sampling. Korean native cattle with initial positives on antigen-ELISA (Ag-ELISA) were sampled again after 3~4 weeks and cattle with second positives in both Ag-ELISA and immunohistochemistry (IHC) were identified as PI cattle. Among the 49 farms, 14 (28.6%) farms had at least more than one PI cow and 21 (0.69%) of 3,050 cattle were determined as PI cattle. As a result of this work, it is suggested that national BVD eradication program is required to reduce economic losses by BVDV infection in Korean cattle industries.

• 대한수의학회지
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• 제32권1호
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• pp.83-90
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• 1992

소 설사병 바이러스 구조단백에 대한 단크론항체를 작성하여 혈청중화시험, 전기영동, 면역침전반응을 이용하여 분석하였던 바 다음의 결과를 얻었다. 중화능력이 있는 항체의 경우 56K내지 54K의 구조단백에 대응하였다. 그외 중화력을 나타내지 않는 항체는 45K와 36K의 바이러스 항원과 대응하였다. 순수정제된 바이러스의 전기영동 분석결과 12종 이상의 바이러스 단백성분이 구조단백질로서 검출되었으며 중화능력을 나타내는 항체를 이용한 면역침전 결과는 이들의 존재를 뒷받침하였다. 중화단백성분의 세포내 전구물질의 검출은 불가능하였으나 방사선동위원소 부착즉시 세포배지에서 바이러스의 존재를 확인할 수 있었다. Staphylococcus aureus $V_8$효소를 이용한 항원의 부분소화 분석결과 45K와 36K의 바이러스 항원은 서로 상관이 있는 것으로서 입증되었다.

• 한국동물위생학회지
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• 제39권1호
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• pp.35-39
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• 2016

The purpose of this study was to survey on infection status of pathogens of diarrhea from Korean indigenous goat. A total of 800 fecal samples was collected from 50 farms from January to October 2015 and was tested by automatic biochemical machine and polymerase chain reaction (PCR). The overall detection ratio of bacterial pathogens was 22.4% and viral pathogens was 16.3%, respectively. The detection ratio of Escherichia coli (E. coli), Salmonella spp., bovine viral diarrhea virus (BVDV), rotavirus and coronavirus were 21.5%, 0.9%, 7.6%, 5.6% and 3.0%, respectively. In the rates of mixed detection, single was 78.2%, double 8.4%, triple 11.6% and quadruple 1.8% in each sample and 38%, 12%, 16%, 20% in each farm, respectively.

• 한국임상수의학회지
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• 제39권1호
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• pp.38-43
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• 2022

A 5-month-old Holstein downer calf was presented, and bovine viral diarrhea virus was detected in the feces and the cerebrospinal fluid. Combined treatment of recombinant bovine somatotropin (rBST) administration and standing rehabilitation had been performed for two months, and the calf could maintain the standing position for a while. However, the symptoms were not improved, and the calf died due to ruminal tympany and aspiration pneumonia. Megaesophagus, abomasal ulcer, and a normal-sized but softened cerebellum were observed in clinical necropsy. Improvement of symptoms was confirmed by rBST, but was not reached the complete recovery of gait and standing position.