• Title/Summary/Keyword: Bovine heart

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Investigation into the Distribution of Total, Free, Peptide-bound, Protein-bound, Soluble-and Insoluble-Collagen Hydroxyproline in Various Bovine Tissues

  • Siddiqi, Nikhat J.;Alhomida, Abdullah S.
    • BMB Reports
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    • v.36 no.2
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    • pp.154-158
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    • 2003
  • Collagen is a family of proteins which consists of several genetically distinct molecular species and is intimately involved in tissue organization, function, differentiation and development. The purpose of this study was to investigate the concentration of different hydroxyproline (Hyp) fractions viz., total, free, peptide-bound, protein-bound, soluble- and insoluble-collagen hydroxyproline (Hyp) in various bovine tissues. Results showed that liver had the highest concentration of free Hyp followed by kidney, brain, spleen, lungs, muscle and heart. Liver also had the highest concentration of peptide-bound collagen Hyp followed by kidney, heart, spleen, lungs, brain and muscle. The concentration of protein-bound collagen Hyp was highest in the liver, followed by kidney, spleen, lungs, muscle, brain and heart. Total Hyp was highest in the liver, followed by kidney, spleen, brain, heart, muscle and lungs. Liver also had significantly high concentration of collagen as compared to other tissues examined (P<0.001). Spleen had the significantly higher concentration of soluble-collagen Hyp when compared to other tissues (P<0.001). This was followed by heart, muscle, lungs, brain, kidney and liver. Heart had the highest concentration of insoluble-collagen Hyp followed by lungs, kidney, liver, muscle, spleen and brain. The variation among the insoluble-collagen Hyp concentration of heart and muscle, spleen and brain was significant (P<0.001). We speculate that these differences could be due to the variation in turn over of rate of collagen metabolism in this species.

Purification and Comparison of NADH-Cytochrome $b_5$ Reductase from Mitochondrial Outer Membrane of Bovine Heart and Turnip

  • 이재양;김영호;이상직
    • Bulletin of the Korean Chemical Society
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    • v.19 no.2
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    • pp.160-164
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    • 1998
  • The NADH-cytochrome b5 reductase (NCBR), a mitochondrial external electron carrier, was purified from bovine heart and turnip and their properties were examined. The mitochondrial outer membranes separated were subjected to NCBR isolation through DEAE-Cellulose ion exchange, DEAE-Sephadex gel chromatography, and hydroxyapatite adsorption chromatography. These processes yielded the purification folds of 88 and 42 and the recovery percentages of 0.2%, 5.67% for turnip and bovine heart, respectively. The molecular weight of the NCBR from the two sources was estimated to be 35,000 using SDS polyacrylamide gel electrophoresis. The Michaelis constant Km and maximum velocity Vmax were determined by measuring the NADH-ferricyanide redox system as well as the NADPH-ferricyanide redox system. The kinetics showed that both NCBRs had higher affinities for NADH than artificial electron-acceptor substrate ferricyanide. Although NADPH had a lower affinity for the enzymes than NADH, this study showed the 2'-phosphate dinucleotide could be used as a substrate.

A Study on the Bovine Sarcocystis -(Report II ):A Survey on the Bovine Sarcocystis in the Muscles of Heart, Liver, Kidney, Tongue, Diaphragm and Masseter - (소의 주근포자충에 관한 연구 -(제 II 보) : 심장, 간장, 신장, 설, 횡격막 및 교근내 주근포자충의 조사연구-)

  • Yoon Hwa-Joong;Ra Seong-An
    • Journal of the korean veterinary medical association
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    • v.24 no.2
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    • pp.92-97
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    • 1988
  • One hundred eighteen cattle in the slaughter houses of kyeong ki-do were examined for Sarcocystis cruzi using macroscopic and microscopic method of detection. Sausage-shaped Sarcocystis were detected from the heart, diaphragm, tongue, masseter, liver, kid

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Bovine abortion associated with neospora in Korea (Neospora에 의한 소 유산 발생)

  • Kim, Dae-yong;Hwang, Woo-suk;Kim, Jae-hoon;Hur, Kwon;Hwang, Ui-Gyeong;Lee, Byeong-chun;Jean, Young-hwa;Rhee, Jae-chin;Choi, Sang-ho
    • Korean Journal of Veterinary Research
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    • v.37 no.3
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    • pp.607-612
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    • 1997
  • A case of neosporosis is described in a 6-month-old aborted bovine fetus. Grossly, numerous, well-demarcated, 2-3mm, yellowish white foci were scattered in the heart and skeletal muscle. Histopathologic changes were confined to the brain, heart, and skeletal muscle. Multifocal areas of necrotizing encephalitis were observed in the cerebrum as well as cerebellum. Severe multifocal to coalescing nonsuppurative inflammation was observed in the myocardium and skeletal muscle. The tachyzoites of Neospora caninum were demonstrated in the brain, myocardium and skeletal muscle with immunohistochemical method using Neospora caninum-specific antibody. Based on the histopathology and immunohistochemistry, this case was diagnosed as neosporosis in aborted Korean cattle. This is believed to be the first reported case of bovine neosporosis in Korea.

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Production of Rabbit Anti-Bovine Heart Pyruvate Dehydrogenase Antibody (피루브산 탈수소 효소(송아지 심장)의 항체(토끼)생산)

  • 권무식
    • KSBB Journal
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    • v.5 no.4
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    • pp.315-321
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    • 1990
  • Rabbit anti-bovine heart PDH antiserum was raised against El(a, b) isolated from PDC, and then applied to detect Ela and Elb. Appropriate amounis of El were fractionated by SDS-PAGE and electrophoretically transferred to nitrocellulose membrane. The Ela and Elb on the membrane were incubated with anti-El antiserum and identified by GAR-HRP system. It has been found that the immunodetection sensitivity of Ela and Elb were directly proportional to the amount of antigen and transfer time. The lengthy transfer times increased the immunodetection sensitivity of Ela and Elb. The maximal detection sensitivity of Western blotting of Ela and Elb was achieved at 3.5 V/cm for 16-hour transfer under these experimental conditions.

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Norwood Procedure with Home-made Bovine Pericardial Patch (우심낭 조직편을 이용한 Norwood 술식 -2예 보고-)

  • 이석기;김웅한
    • Journal of Chest Surgery
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    • v.36 no.8
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    • pp.602-605
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    • 2003
  • A patch design using bovine pericardial patch for aortic enlargement in the Nowood procedure has been introduced to avoid problems related to homograft availability. We report 2 successful cases of Norwood procedure with home-made bovine pericardial patch. The first case was a 23-day-old (2.2 kg) patient with multi-level left ven-tricular outflow tract obstruction with ductal-dependent systemic circulation. The other case was a 9-day-old (3 kg) patient with hypoplastic lefi heart syndrome. This technique was relatively easy to perform, reproducible and quite effective like homograft patch.

A Survey of Sarcocystis Infections in the Slaughtered Cattle and Identification of Sarcocystis cruzi (도축우의 심장근육내 주육포자충 감염실태조사와 Sarcocystis cruzi의 동정)

  • 박양주;김종술;정동수;박양순;신명균;김교승
    • Korean Journal of Veterinary Service
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    • v.15 no.2
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    • pp.109-120
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    • 1992
  • 330 Samples of the slaughtered cattle heart muscle were collected from the abattoirs of five regions in Kangwon - do to reveal the frequency of sarcocystis infections during January through December in 1991. The samples were inspected for bradyzoites by the trypsin digestion technique and the possitive samples were fed to dogs and cats for the detection of sporocysts shed in the feces. The results obtained were summarized as follows : 1. The infection rate of bovine Sarcocystis investigated from 330 samples was 43.6%. 2. It revealed that the infection rate of Sarrocystis increased gradully with the advance in the age, 14.5% in below two years, 26.1% in the three years, 30% in four years, 54.7% in five years, 74.4% in six years, 90% in seven years and 100% in older than eight years. 3. The cyst walls detected out from the heart muscles were less than l${\mu}{\textrm}{m}$ in thickness and the size of bradyzoites were $11.8{\times}2.8{\mu}m$ in average. 4. The size of sporocysts shed in the feces of dogs were $15.8{\times}9.8{\mu}m$ in average and the prepatent periods ranged from 12 to 16days. 5. Sarcorystis found in the bovine heart muscles were identified as Sascocystis cruzi ( Hasselman, 1923) , wenyon, 1926.

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Properties of Frankfurter-type Sausages with Pork Back-fat Replaced with Bovine Heart Surimi-like Materials

  • Seo, Jin-Kyu;Yum, Hyeon-Woong;Kim, Gap-Don;Jeong, Jin-Yeon;Yang, Han-Sul
    • Food Science of Animal Resources
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    • v.36 no.4
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    • pp.523-530
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    • 2016
  • This study investigates the effect of bovine heart surimi-like material (BHSM) used as a back fat replacer, on the physicochemical and sensory characteristics of frankfurter-type sausages. Frankfurter-type sausage with added BHSM had a higher moisture content and lower fat content than the control. In addition, the samples with added BHSM had higher pH, cooking loss and 2-thiobarbituric acid-reactive substance (TBARS) value and lower water exudation than the control. The sausage formulation with 40% BHSM was more effective in delaying lipid oxidation without affecting cooking loss compared to the 60% BHSM treatment sample. Results showed that hardness values increased upon replacement with BHSM, and sausages manufactured with 40% BHSM had higher lightness and lower redness values. Panelists found there were no differences in color, odor, and tenderness scores and the overall acceptability score found that treatment samples containing 20% and 40% BHSM were preferable to the control after storage for 14 d. These results indicate that fat replacement with BHSM was beneficial to the quality of frankfurter-type sausages, and acceptable reduced-fat products can be produced when back fat is replaced with up to 40% BHSM.