• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.27-27
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• 2015

Clubroot (Plasmodiophora brassicae) is a serious agricultural problem affecting Brassica crop production worldwide. It also infects the model plant Arabidopsis thaliana. During infection, this biotrophic pathogen manipulates the development and metabolism of its host leading to the development of galls in the root and hypocotyl. In turn, its own development is strongly influenced by the host. The aim of this study is to investigate the metabolism of clubroot-infected plants using a combination of transcriptomic and metabolomic approaches. We have used direct injection mass spectrometry to obtain a metabolic fingerprint of when changes in the metabolome occur and linked this with changes in host and pathogen gene expression. We have identified alterations in carbohydrate metabolism that occur during P. brassicae infection of A. thaliana plants. Transcriptomic analysis showed that host genes associated with sugar transport and metabolism were induced during gall formation and that the pathogen also expresses genes associated with these processes. We have examined the impact of inactivating host sucrose synthase, cytosolic invertase and sugar permeases on gall formation, identifying host genes that are required for gall formation. We have also explored how sugar status is changed in root tissue, developing and mature leaf during infection of wild type and mutant plants.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.2
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• pp.95-104
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• 1999

For the using as the guideline to evaluate the grassland based on the botanical composition, the ecological behaviour which was expressed of nine(1~9:reaction and nitrogen figure) or twelve(1~12:moisture figure) division scale was given to the each plant species in grassland. At first, the ecological behaviours(moisture, reaction and nitrogen figure) were given to the 141 main forage plants of grassland in Korea. In the improved grassland, moisture, reaction and nitrogen figure of the ecological behaviour were much more increased 0.73, 0.94 and 0.95 than those of the waste type grassland, respectively. Therefore, the ecological behaviour of grassland was not only possible to express the vegetational conditions of various plant communities in some simple figures, but also compared with different types of grassland at the same time.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.11
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• pp.1611-1616
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• 2002

The alkanes in plant materials can potentially be used as markers to estimate composition and digestibility of diet of deer for the development of feeding strategies, but the analysis of alkanes in plant materials and deer faeces is time-consuming and expensive. In this study, 242 faecal samples and 119 pasture samples were scanned using near infrared spectroscopy (NIR) and the concentrations of alkanes in these samples were analysed to develop calibrations for predicting alkane concentrations in pastures and deer faeces. The $R^2$ values for NIR calibrations were <0.6 for $C_{24}$, $C_{26}$, $C_{32}$ and $C_{36}$, but were >0.8 for other alkanes for faecal samples. The $R^2$ values were >0.87 for alkanes with chains from $C_{27}$ to C35 for pasture samples. However, NIR was unable to predict concentrations of alkanes with chains of $C_{24}$, $C_{26}$, $C_{32}$ and $C_{36}$ in faecal samples and $C_{24}$, C25, $C_{26}$ and $C_{36}$ in pasture samples. While the use of these NIR calibrations will accelerate the estimation of diet digestibility, dietary components and botanical composition of pastures, the influence of the type of pasture on NIR calibration will require further examination.

• Korean Journal of Plant Taxonomy
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• v.46 no.3
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• pp.295-300
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• 2016

Sisyrinchium micranthum Cav., a newly naturalized species, was found in Seogwipo, Jeju-do. This species is native to Central and South America and is reported as a widely invasive species around the world. Sisyrinchium micranthum is a sister species of S. rosulatum E. P. Bicknell (Deung-Sim-But-Kkot in Korean), reported as an invasive species earlier in Korea, and both species are members of the subgenus Echthronema. Sisyrinchium micranthum is distinguished from S. rosulatum by the urceolate shape of its flowers and by the large size of its fruits and seeds. Its local name is "Yeon-Deung-Sim-But-Kkot" based on the light blue corolla. We also found that Sisyrinchium angustifolium Mill., previously used for Deung-Sim-But-Kkot, was misapplied. It belongs to the subgenus Sisyrinchium and is morphologically very different from S. rosulatum. We corrected the scientific name of Deung-Sim-But-Kkot by changing it from S. angustifolium to S. rosulatum based on the type specimens, original descriptions, and recent studies.

• The Korea Journal of Herbology
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• v.26 no.1
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• pp.1-6
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• 2011

Objectives : Due to morphological similarity, many substitutes and counterfeits have been used as Moktong for thousands of years. This study was designed to determine discriminative criteria for identifying medicinal herbs used as Moktong. Methods : Akebia quinata, A. trifoliata, Clematis armandii, and Aristolochia manshuriensis were collected in various regions. Samples were authenticated and examined by observation and microscopy with paraffin embedding and triple staining using the modified Ju staining method. Results : Three different types of features to establish discriminative criteria for four kinds of Moktong were identified. Botanical features include differences in phyllotaxy, leaf shape, and caulescent. External morphological features were arrangement and size of fine holes(xylem), and overall shape of the section. Internal morphological features include the number of medullary rays, shape of the pitch, type of tissues rounding pitch, appearance of annual rings, shape and amount of crystals(calcium-oxalate), and the appearance of cork cambium. Further details(e.g. identification keys) are in the article. Conclusions : These criteria could provide an effective method for identifying numerous kinds of Moktong distributed in markets throughout northeast Asian nations.

• Journal of Species Research
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• v.4 no.2
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• pp.159-168
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• 2015

Although airborne pollen is invisible to the eye, it has been known as a major source to respiratory allergic reactions. For this reason, airborne pollen is monitoring in many countries to predict pollen concentration based on locality and season. However, the morphological characteristics of airborne pollen and their potential tendency as an allergen are still obscure. In the present study, we selected 52 airborne pollen samples based on previously reported data and investigated their detail pollen characteristics using LM and SEM. Major airborne pollen in Korea has sorted in 19 families (most angiosperms except four gymnosperm families), and all pollen grains are small to medium in size ($P=17.34-49.86{\mu}m$) apart from the bisaccate pollen grains of Pinaceae ($P=46.49-106.20{\mu}m$). The aperture number and shape vary from sulcate to polyporate. While the inaperture pollen has found only in gymnosperm (Cupressaceae and Taxaceae), triporate or polyporate is common pollen type in angiosperm. The sexine ornamentations could divide into several types, but the most sculpturing types are inconspicuous like psilate, rugulate and granulate. Reticulate pollen grains as a semitectum have occurred the species of genera Platanus and Fraxinus only. To estimate the possible relationships between pollen features and allergen, the results are discussed in botanical context.

• Korean Journal of Pharmacognosy
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• v.49 no.2
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• pp.145-154
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• 2018

Aconiti Kusnezoffii Tuber has been traditionally used to treat the symptoms of rheumatoid arthritis and joint pain. The main constituents are diterpenoid alkaloids such as benzoylmesaconine, benzoylaconine, mesaconitine, aconitine, and hypaconitine. In Korea, Aconiti Kusnezoffii Tuber is officially defined as the tubers of Aconitum kusnezoffii Reichb., A. ciliare Decasisne, and A. triphyllum Nakai. On the other hand, only the tuber of A. kusnezoffii is to be used in China. In order to identify the botanical origin of Aconiti Kusnezoffii Tuber circulated in Korea, we analyzed 24 samples of Aconiti Kusnezoffii Tuber obtained from local markets for comparative DNA analysis. The sequence analysis of nrRNA ITS 1 was useful to distinguish Aconitum species and revealed that the roots of A. karakolicum were circulated in Korean markets without discretion. HPLC quantitative analysis showed that aconitine was detected at the highest amount in A. karakolicum. Authentic diterpenoid alkaloids were coinjected for quantification of aconitine-type ingredients. All data were statistically grouped by Principal Component Analysis (PCA). This study suggests that both molecular and chemical analyses should be utilized for the standardization and the quality control for Aconiti Kusnezoffii Tuber.

• Proceedings of the Botanical Society of Korea Conference
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• 1999.07a
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• pp.51-56
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• 1999

Plastids, which are organelles unique to plant cells, bear their own genome that is organized into DNA-protein complexes (nucleoids). Regulation of gene expression in the plastid has been extensively investigated because this organelle plays an important role in photosynthesis. Few attempts, however, have been made to characterize the regulation of plastid gene expression at the chromosomal structure, using plastid nucleoids. In this report, we summarize the recent progress in the characterization of DNA-binding proteins in plastids, with special emphasis on CND41, a DNA binding protein, which we recently identified in the choloroplast nucleoids from photomixotrophically cultured tobacco cells. CND41 is a protein of 502 amino acids which consisted of a transit peptide of 120 amino acids and a mature protein of 382 amino acids. The N-terminal of the 'mature' protein has lysine-rich region which is essential for DNA-binding. CNA41 also showed significant identities to some aspartyl proteases. Protease activity of purified CND41 has been recently confirmed and characterized. On the other hand, characterization of accumulation of CND41 both in wild type and transgenic tobacco with reduced amount of CND41 suggests that CND41 is a negative regulator in chloroplast gene expression. Further investigation indicated that gene expression of CND41 is cell-specifically and developmentally regulated as well as sugar-induced expression. The reduction of CND41 expression in transgenic tobacco also brought the stunted plant growth due to the reduced cell length in stem. GA3 treatment on apical meristem reversed the dwarf phenotype in the transformants. Effects of CND41 expression on GA biosynthesis will be discussed.

• Korean Journal of Plant Taxonomy
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• v.45 no.2
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• pp.178-182
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• 2015

Stellaria sessiliflora Y. Yabe (Caryophyllaceae), which is known to be distributed throughout Japan excluding Okinawa, was recently found on Jeju Island in Korea. Reports on the Japanese flora state that this species is distributed on Jeju Island, and a photograph of one specimen (Slide No. 6294) collected from Jeju Island by Ohwi in May 1935 confirms this. Additionally, specimens at the National Arboretum and Korea Forest Research Institute indicate that S. sessiliflora has occurred on Jeju Island for a considerable amount of time. However, the species has never been officially listed in the flora of Korea. Stellaria sessiliflora is distinguished from S. media by having broader leaves with a slightly undulate margin, petiolate leaves on the upper part of the stem, and 10 stamens. The local name 'Geu-Neul-Byeol-Kkot' is given to the species based on its shady habitat under deciduous broadleaf forest. Details of the morphological characters, an illustration, the type specimen, voucher specimen data, and photographs of Stellaria sessiliflora in Korea are presented.

• Korean Journal of Plant Taxonomy
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• v.46 no.4
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• pp.348-355
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• 2016

We report previously unrecognized flowering plants of Lao PDR found during a botanical survey of Lao PDR in 2015-2016. We found Phyllagathis tuberosa (C. Hansen) Cellinese & S. S. Renner (Melastomataceae) and Tolypanthus pustulatus Barlow (Loranthaceae) in the Phou Khao Khouay National Biodiversity Conservation Area (PKKNBCA), previously only known from type localities in Thailand. This is the first report of Phyllagathis tuberosa and the genus Tolypanthus in the Lao PDR. We also found Lonicera bournei Hemsl. (Caprifoliaceae) in the proposed Phosabous National Protected Area (PNPA) of Xiangkhoang, neglected with regard to the flora of Laos since its initial report in early 20th century. The current report will augment our understanding of the floristic relationships among SE Asian countries and will help elucidate the biodiversity of Lao PDR.