• Natural Product Sciences
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• v.26 no.2
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• pp.132-135
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• 2020

Volatile constituents obtained by water distillation from the aerial parts and root of Onosma dichroanthum Boiss (Boraginaceae) native to the north of Iran were investigated by GC and GC/MS for the first time. Palmitic acid (39.61%) and decane (31.39%) were the major components in the root while decane (26.26%) and phytol (25.52%) were the predominant constituents in the aerial parts. Ketones, aldehydes, alkanes, fatty acids, oxygenated diterpenes and sesquiterpenes were characterized as the most phytochemicals in the aerial parts. Alkanes and fatty acids were identified as the main groups in the root volatile substances. There were two ketone derivatives, belong to apocarotenoids, in the aerial parts; β-ionone and hexahydrofarnesyl acetone.

• YAKHAK HOEJI
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• v.59 no.5
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• pp.222-229
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• 2015

Lithospermi radix, the dried roots of Litospermum erythrorhizon Sieboid et Zuccarini (Boraginaceae), has long been used to treat detoxification and inflammation. In this study, we isolated two main quinoid compounds, ${\beta}$-hydroxyisovalerylshikonin (1) and acetylshikonin (2) from L. erythrorhizon. As acetylshikonin is considered as a marker compound of L. erythrorhizon, a rapid analysis method for the simultaneous determination of quinoid compounds including 2 was also developed by HPLC (High Performance Liquid Chromatography) and validation of this analytical method. By the developed method, two quinoid marker compounds (${\beta}$-hydroxyisovalerylshikonin and acetylshikonin) were successfully quantified in 31 commercial samples which were collected from different regions. The contents were 0.20% (${\beta}$-hydroxyisovalerylshikonin) and 0.22% (acetylshikonin), respectively.

• Archives of Pharmacal Research
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• v.28 no.4
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• pp.400-404
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• 2005

Activity-guided fractionation of a hexane-soluble extract of the roots of Lithospermum erythrorhizon, using a mouse brain monoamine oxidase (MAO) inhibition assay, led to the isolation of two known naphthoquinones, acetylshikonin and shikonin, and a furylhydroquinone, shikonofuran E. These compounds were shown to inhibit MAO with $IC_{50}$ values of 10.0, 13.3, and $59.1 {\mu}M$, respectively. Although no specificity for MAO-A and MAO-B was shown by acetylshikonin and shikonin, a Lineweaver-Burk plot analysis indicated that the inhibition was competitive for both MAO-A and MAO-B activity.

• Korean Journal of Plant Taxonomy
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• v.47 no.3
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• pp.199-206
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• 2017

The last four years of joint botanical collections by the governments of Myanmar and South Korea in Natma Taung National Park and adjacent areas in the Chin State of Myanmar have revealed the presence of 20 naturally occurring species of angiosperms new to the flora of Myanmar. Plants not previously recorded include species originally considered to be only found in neighboring mega-diverse countries. Examples (e.g., for India) include Boehmeria manipurensis Friis & Wilmot-Dear (Urticaceae), Trigonotis hookeri Benth. ex C. B. Clarke (Boraginaceae) and Mycetia radiciflora (C. B. Clarke) Airy Shaw (Rubiaceae); those for China include Microtoena delavayi Prain (Lamiaceae), Pimpinella kingdon-wardii H. Wolff (Apiaceae) and Senecio diversipinnus Y. Ling (Asteraceae). The data presented in this report are expected to be useful sources for phytogeographical studies of these species.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.6
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• pp.789-795
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• 2010

In order to set up an accurate quality criteria for the Boraginaceae that have been traditionally used for medical purposes and food colorant, and to assess its viability as functional food ingredient, antioxidant tests were conducted on the wild and cultivated plants. Variety of indicators including total contents of phenol, DPPH, SOD-liked effect, hydroxy radical-scavenging effect, lecithin oxidation inhibitory effect, etc were analyzed. Wild and cultivated gromwell's total contents of phenol in their methanol extracts were 0.14% and 0.13%, while they were most active in ethyl acetate extracts and n-hexane extracts, respectively. $IC_{50}$ values of methanol extract of the wild and cultivated plants were 794.41 ${\mu}g$/mL and 971.86 ${\mu}g$/mL, indicating that the wild plant is more responsive (p<0.05) to low concentration. Also the wild and cultivated plants were most active in ethyl acetate fraction and n-hexane extracts when their $IC_{50}$ values were measured by each solvent extracts. SOD-liked effects of both plants were concentration dependent while methanol extracts were more active (p<0.05) in 500 ${\mu}g$/mL than other solvent extracts. Hydroxy radical-scavenging effect of both plants showed less than 50% activity in concentration of 1,000 ${\mu}g$/mL except in chloroform fraction and n-hexane fraction. Lecithin oxidation inhibitory effects of the wild and cultivated plants were active in methanol and solvent extracts of 200~1000 ${\mu}g$/mL. Especially it showed 90% of high inhibitory effect in 1,000 ${\mu}g$/mL of chloroform fraction. Hence, both wild and cultivated Boraginaceae were analyzed to be viable as functional food ingredient.

• Korean Journal of Plant Resources
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• v.25 no.6
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• pp.753-761
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• 2012

We performed phylogenetic analyses of a total of 21 acessions covering 5 species in the Korean Trigonotis and one outgroup species using nuclear ribosomal ITS and chloroplast rbcL, matK, ndhF sequences. Outgroup were chosen from the closely related genus Lithospermum zollingeri. Both parsimony and Bayesian Inference methods were used to reconstruct the evolutionary history of the group. The evidence collected indicated that phylogenetic relationships among Korean Trigonotis species are unresolved based on nuclear marker (ITS), as the same as based on separated chloroplast sequences. While the phylogenetic relationships of Korean Trigonotis species almost clearly were resolved in combined chloroplast sequences. Thus, the members of Trigonotis coreana can be distinguished to the members of Trigonotis peduncularis in combined cpDNA sequences and Trigonotis nakaii was treated as a synonymed to Trigonotis radicans var. sericea. In addition, the MP and BI analysis showed Trigonotis icumae as sister of the remained Korean Trigonotis species based on combined molecular markers (BI: PP = 1).

• Korean Journal of Medicinal Crop Science
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• v.18 no.5
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• pp.311-314
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• 2010

The content of luteolin in the different parts of Lithospermum erythrorhizon was determined by high performance liquid chromatography (HPLC). Luteolin was quantified by a reverse-phase column with gradient solvent program (water : ACN = 90 : 10 to 77 : 23 for 60 min). UV detection was conducted at 330 nm. The content of luteolin was measured in the seeds (0.28 mg/g), stems (0.30 mg/g), and roots (0.16 mg/g) of L. erythrorhizon. The presence of luteolin in Lithospermum species is important in agricultural crop production for increasing the amounts of clinically available medicine and health supplements.

• The Korean Journal of Physiology and Pharmacology
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• v.23 no.6
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• pp.501-508
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• 2019

Rosmarinic acid (RA) is a natural polyphenolic compound that exists in many medicinal species of Boraginaceae and Lamiaceae. The previous studies have revealed that RA had therapeutic effects on hepatocellular carcinoma (HCC) in the H22-xenograft models by inhibiting the inflammatory cytokines and $NF-{\kappa}B$ p65 pathway in the tumor microenvironment. However, its molecular mechanisms of immunoregulation and pro-apoptotic effect in HCC have not been fully explored. In the present study, RA at 75, 150, and 300 mg/kg was given to H22 tumor-bearing mice via gavage once a day for 10 days. The results showed that RA can effectively inhibit the tumor growth through regulating the ratio of $CD4^+/CD8^+$ and the secretion of interleukin (IL)-2 and interferon-${\gamma}$, inhibiting the expressions of IL-6, IL-10 and signal transducer and activator of transcription 3, thereby up-regulating Bax and Caspase-3 and down-regulating Bcl-2. The underlying mechanisms involved regulation of immune response and induction of HCC cell apoptosis. These results may provide a more comprehensive perspective to clarify the anti-tumor mechanism of RA in HCC.

• Korean Journal of Plant Taxonomy
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• v.52 no.3
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• pp.173-183
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• 2022

Through the collection of herbarium specimens in Bhutan over the years, new plant species are discovered nearly annually. Thus, this paper reports two new genera and 19 new records of flowering plant species from Bhutan. The new genera include Eurycorymbus of the family Sapindaceae and Homalium of the family Salicaceae. The new records of plant species are Eranthemum erythrochilum (Acanthaceae), Hemidesmus indicus (Apocynaceae), Ilex umbellulata (Aquifoliaceae), Canarium strictum (Burseraceae), Ehretia acuminate (Boraginaceae), Vaccinium sikkimense (Ericaceae), Nothapodytes foetida (Icacinaceae), Machilus edulis (Lauraceae), Grewia asiatica (Malvaceae), Hibiscus fragrans (Malvaceae), Cipadessa baccifera (Meliaceae), Baccaurea javanica (Phyllanthaceae), Canthiumera glabra (Rubiaceae), Homalium napaulense (Salicaceae), Eurycorymbus cavaleriei (Sapindaceae), Acmella radicans (Asteraceae), Silene latifolia (Caryophyllaceae), Cleome rutidosperma (Cleomaceae), and Cuphea carthagenensis (Lythraceae). Morphological determinations of the genera and species were carried out at the National Herbarium (THIM) of the National Biodiversity Centre of Bhutan. Brief descriptions of the species, phenology, and photo plates are provided in this annotated checklist.

• Korean Journal of Plant Resources
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• v.25 no.1
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• pp.31-47
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• 2012

Distributional surveys for vascular plants were carried out in Daecheong island, Ongjin-gun, South Korea, from 2008 to 2010. The vascular plants recognized from the island were in tatal 402 taxa that were of 90 families, 269 genus, 350 species, 3 subspecies, 43 varieties, 6 forms. Among the investigated 402 taxa, 2 Korean endemic plants, 8 rare and endangered plants which was designated by Korea Forest Service, 35 specially plants designated by the Ministry of Environment were included. The naturalized plants were identified as 40 taxa and the percentage of naturalized plants index was 9.9%. In addition, an unrecorded species, Thyrocarpus glochidiatus Maxim (Boraginaceae), was recognized from the island.