• 제목/요약/키워드: Bone graft substitute$\beta$-TCP

검색결과 7건 처리시간 0.021초

대식세포가 β-tricalcium Phosphate 뼈이식제의 생분해에 미치는 영향 (Effects of Macrophage on Biodegradation of β-tricalcium Phosphate Bone Graft Substitute)

  • 김영희;;변인선;오익현;민영기;양훈모;이병택;송호연
    • 한국세라믹학회지
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    • 제45권10호
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    • pp.618-624
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    • 2008
  • Various calcium phosphate bioceramics are distinguished by their excellent biocompatibility and osteoconductivity. Especially, the exceptional biodegradability of $\beta$-TCP makes it a bone graft substitute of choice in many clinical applications. The activation of osteoclasts, differentiated from macrophage precursor cells, trigger a cell-mediated resorption mechanism that renders $\beta$-TCP biodegradable. Based on this evidence, we studied the biodegradation process of granular-type $\beta$-TCP bone graft substitute through in vitro and in vivo studies. Raw 264.7 cells treated with RANKL and M-CSF differentiated into osteoclasts with macrophage-like properties, as observed with TRAP stain. These osteoclasts were cultured with $\beta$-TCP nano powders synthesized by microwave-assisted process. We confirmed the phagocytosis of osteoclasts by observing $\beta$-TCP particles in their phagosomes via electron microscopy. No damage to the osteoclasts during phagocytosis was observed, nor did the $\beta$-TCP powders show any sign of cytotoxicity. We also observed the histological changes in subcutaneous tissues of rats implanted with granule-type $\beta$-TCP synthesized by fibrous monolithic process. The $\beta$-TCP bone graft substitute was well surrounded with fibrous tissue, and 4 months after implantation, 60% of its mass had been biodegraded. Also, histological findings via H&E stain showed a higher level of infiltration of lymphocytes as well as macrophages around the granule-type $\beta$-TCP. From the results, we have concluded that macrophages play an important role in the biodegradation process of $\beta$-TCP bone graft substitutes.

The BMPs expression and histomorphometric study of ${\beta}-TCP$ / rhBMP-2 Grafting on the rabbit cranial bone defects

  • Lim, Byung-Sup;Jeon, Jae-Yoon;Park, Chang-Joo;Im, Jae-Jung;Hwang, Kyung-Gyun;Shim, Kwang-Sup
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • 제34권1호
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    • pp.49-58
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    • 2008
  • Objective: The Purpose of the study was to investigate the bone morphogenic protein expression of rhBMP-2(recombinant human bone morphogenic protein-2) as singnaling molecule and ${\beta}-TCP$(Tricalcium phosphate) as a bone substitute and carrier medium of rhBMP-2. Materials and Methods: 16 rabbits divided into 2 group of each 8 rabbit. Two standardized bone defect, round bilateral defect was made in the cranium of the 8 rabbit of first group, and was grafted with $150{\sim}500{\mu}m$ diameter ${\beta}-TCP$ 0.25g in one side, which was soaked with rhBMP-2, and autogenous bone was grafted on another side as a positive control. Second group of 8 rabbit, only ${\beta}-TCP$ was grafted with same size and same manner. After 2, 4, 8, and 12 weeks, specimen was taken for microscopic immunohiostochemical and histomorphometric analysis. Result: Grafting ${\beta}-TCP$ with rhBMP show the early formation of the bone regenerative factor (BMP-4) and more quantity of new bone formation than only use of ${\beta}-TCP$ (8,12 week), even show less new bone formation than autogenous bone. Conclusion : The experimental study result that ${\beta}-TCP$ graft combination with rhBMP-2 as a delivery system is an effective with osteoinductive capacity and biodegradable properties, so that provide clinical availibility of composite use in reconstruction of bony defect.

정형외과 수술에서 골대체제로써 Beta-Tricalcium Phosphate 사용에 대한 방사선학적 결과 (Radiologic Outcome of Beta-Tricalcium Phosphate as a Bone Substitute in Orthopaedic Surgery)

  • 박형석;문정은;조용진
    • 보건의료산업학회지
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    • 제13권4호
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    • pp.133-144
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    • 2019
  • Objectieves: 본 논문의 목적은 정형외과 수술 중 골결손에 대하여 β-TCP를 이용하여 치료 받은 대상자의 방사선학적 결과를 평가하기 위함이다. Methods: 총 49명의 대상자 중 24명 (49.0%)는 남자 였으며, 25명 (51%)는 여자였다. 요인 분석을 위하여 의무 기록 검토가 시행되었다. 각 시기별로 골대체재의 흡수 정도는 Irwin 의 방사선학적 체계에 따라서 평가되었다. Results: 술 후 3개월 째, 세 가지 측정 변수인 골대체제 이식 견고성, 진단 당시 나이, 병변의 부피에 의한 회귀 모형의 설명력은 65.6% 였다. 술 후 6개월 째, 두 가지 측정 변수인 병변의 부피, 골대체제 이식 견고성에 의한 회귀 모형의 설명력은 32.9% 였다. 술 후 9개월 째, 두 가지 측정 변수인 병변의 장축 길이, 골대체제 이식 견고성에 의한 회귀 모형의 설명력은 30.8% 였다. Conclusions: 골대체제 이식 견고성, 병변의 부피, 진단 당시 나이 그리고 병변의 장측 길이가 골대체제의 흡수에 영향을 미치는 인자였다.

폴리머 적층 시스템을 이용한 β-TCP 혼합 비율에 따른 PCL/β-TCP 인공지지체의 제작 (Fabrication of Blended PCL/β-TCP Scaffolds by Mixture Ratio of β-TCP using Polymer Deposition System)

  • 하성우;김종영
    • 한국정밀공학회지
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    • 제31권9호
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    • pp.791-797
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    • 2014
  • Abstract Scaffold used as a carrier of the cell has been actively conducted using plenty of technology in tissue engineering. ${\beta}$-tricalcium phosphate (${\beta}$-TCP) material has shown good biocompatibility and osteoconductive ability when it was implanted as a bone graft substitute in osseous defect in human and animal studies for bone regeneration. In this study, we fabricated the blended polycaprolactone (PCL) and ${\beta}$-TCP scaffold by the polymer deposition system (PDS). The PCL/${\beta}$-TCP scaffold was fabricated at a temperature of $110^{\circ}C$, pressure of 650 kPa, and scan velocity of 100 mm/sec. The Overall geometry and size of the scaffold were fixed circle type with a diameter of 10 mm and a height of 4 mm. PCL/${\beta}$-TCP scaffold was observed by scanning electron microscopy. Cell attachment and proliferation of the scaffold containing 30 wt% ${\beta}$-TCP was superior to those containing 10 wt% and 20 wt% ${\beta}$-TCP.

가토 상악동에 이식된 ${\beta}$-TCP의 골치유에 관한 실험적 연구 (THE EXPERIMENTAL STUDY OF THE BONE REGENERATION ON RABBIT MAXILLARY SINUS GRAFTING WITH ${\beta}$-TCP)

  • 박정하;황경균;박창주;최용수;마평수;백승삼;심광섭
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • 제32권2호
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    • pp.107-116
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    • 2006
  • Purpose:Maxillay sinus grafting is an effective treatment procedure to improve bone height in the posterior maxillar area for implant installation. Beta-tricalciumphosphate(${\beta}$-TCP) was introduced to be grafting substitute material, providing a reasonable bio-degradation time, no need for harvesting procedure. The purpose of this study is to evaluate bone healing & regeneration phase using histomorphometric and immunohistochemical analysis. Material & Methods:Sixteen rabbits were divided into 4 groups. Bi-lateral maxillary sinus membranes were elevated at each rabbits, ${\beta}$-TCP was augmented in left sinus, autogenous bone was augmented in right sinus. The rabbits were sacrificed at 2, 4, 8 and 12 weeks. We investigated the bone regeneration & growth factor expression. Results: 1. The mean new bone volume formation was 28.99${\pm}$6.55%, 49.54${\pm}$5.47%, 69.09${\pm}$8.90% in autogenous grafted area, and 22.86${\pm}$5.56%, 24.00${\pm}$4.09%, 34.11${\pm}$3.37% in ${\beta}$-TCP area at 4, 8, 12 weeks. Therefore, new bone formation in autogenous bone was significantly higher than ${\beta}$-TCP (p<0.05). 2. The BMP 2/4 expression in autogenous bone grafted area was higher at 4, 8 weeks. 3. There was no difference in expression pattern of BMP-7/PDGF/VEGF during grafted bone regeneration. Conclusion:The authors we conclude that the autogenous bone graft was faster than ${\beta}$-TCP in bone regeneration, and the BMP 2/4 were more important in graft bone regeneration.

PMMA를 이용한 다공질 β-TCP 골충진제 제조 및 생체적합성 평가 (Fabrication of Porous β-TCP Bone Graft Substitutes Using PMMA Powder and their Biocompatibility Study)

  • 송호연;윤민호;김영희;민영기;양훈모;이병택
    • 한국재료학회지
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    • 제17권6호
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    • pp.318-322
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    • 2007
  • Porous ${\beta}-tricalcium$ phosphate $({\beta}-TCP)$ bioceramic was fabricated by pressureless sintering using commercial HAp and different volume percentages of PMMA powders (30-60 vol.%). The range of spherical pore size was about $200-250\;{\mu}m$ in diameter. By increasing the PMMA content, the number of pores and their morphology were dramatically changed as well as decreased the material properties. In case of using 60 vol.% PMMA content, network-type pores were found, due to the necking of the PMMA powders. The values of relative density, elastic modulus, bending strength and hardness of the 60 vol.% PMMA content sample, sintered at $1500^{\circ}C$, were about 46%, 22.2 GPa, 5MPa and 182 Hv respectively. Human osteoblast-like MG-63 cells and osteoclast-like Raw 264.7 cells were well grown and fully covered all of the porous ${\beta}-TCP$ bodies sintered at $1500^{\circ}C$.

합성골 이식재인 Macroporous biphasic calcium phosphate를 이용한 사람의 상악동 거상술-증례 보고 (Maxillary Sinus Augmentation Using Macroporous Biphasic Calcium Phosphate ($MBCP^{TM}$) : Three Case Report With Histologic Evaluation)

  • 이지현;정의원;김창성;최성호;조규성
    • Journal of Periodontal and Implant Science
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    • 제36권2호
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    • pp.567-577
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    • 2006
  • Background Several bone grafting materials have been used in sinus augmentation procedures. Macroporous Biphasic Calcium Phosphate($MBCP^{TM}$) consists of the mixture of 60% HA and 40% ${\beta}-TCP$. Therefore, it can provide good scaffold for the new bone to grow owing to HA, in the other hand, it can have bioactivity for bone remodeling owing to ${\beta}-TCP$. The purpose of this study was to evaluate bone formation following maxillary sinus augmentation using $MBCP^{TM}$ by means of histologic analysis. Material and Method $MBCP^{TM}$ was placed as a primary bone substitute for maxillary sinus augmentation. Three patients were selected after evalaution of their medical dental examination. $MBCP^{TM}$ only, $MBCP^{TM}$ combined with Irradicated cancellous bone and $MBCP^{TM}$ combined with autogenous bone were used for each patient. After about eight months, bone biopsies were harvested for histologic evaluation and fixtures installed. Results Eight months after surgery we observed new vital bone surrounding $MBCP^{TM}$ particle and the amount of new bone was about 30% even though there were discrepancies between specimens. This case report documents that $MBCP^{TM}$ when used as a grafting material for sinus floor augmentation whether combined other bone graft material or not, may lead to the predictable results for dental implants on posterior maxillary area with insufficient vertical height for fixture installation.