• Journal of Pharmaceutical Investigation
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• v.40 no.2
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• pp.101-107
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• 2010

A rapid and sensitive reversed-phase high performance liquid chromatography (HPLC) method was developed for the determination of N-(-4-Chlorophenyl)-6-hydroxy-7-methoxy-2-chromanecarboxamide (KAL-1120), a novel anti-inflammation agent, in the rat plasma. The method was applied to analyze the compound in the biological fluids such as bile, urine and tissue homogenates. After liquid-liquid extraction, the compound was analyzed on an HPLC system with ultraviolet detection at 275 nm. HPLC was carried out using reversed-phase isocratic elution with a $C_{18}$ column, a mobile phase of a mixture of acetonitril (40 v/v%) at a flow rate of 1.0 mL/min. The chromatograms showed good resolution and sensitivity and no interference of plasma. The calibration curve for the drug in plasma was linear over the concentration range of 0.05-50 ${\mu}g$/mL. The intra- and inter-day assay accuracies of this method ranged from 0.06% to 9.33% of normal values and the precision did not exceed 6.28% of relative standard deviation. The plasma concentration of KAL-1120 decreased to below the quantifiable limit at 1.5 hr after the i.v. bolus administration of 2-10 mg/kg to rats ($t_{1/2,({\alpha})}$ and $t_{1/2,({\beta})$ of 2.15 and 26.7 min at a dose of 2 mg/kg, 3.91 and 33.0 min at a dose of 10 mg/kg, respectively). The steady-state volume of distribution ($V_{dss}$) and the total body clearance ($CL_t$) were not significantly altered in rats given doses from 2 to 10 mg/kg. Of the various tissues tested, KAL-1120 was mainly distributed in the lung and heart after i.v. bolus administration. KAL-1120 was detected in the bile by 30 min after its i.v. bolus administration. However, the concentration in the urine after i.v. bolus administration became too low to measure, suggesting that KAL-1120 is mostly excreted in the bile. In conclusion, this analytical method was suitable for the preclinical pharmacokinetic studies of KAL-1120 in rats.

• Journal of the Korean Society of Clothing and Textiles
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• v.3 no.1
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• pp.31-47
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• 1979

1. The old Korean costume had two different kind of dress, one was a military uniform (Goon bok) for military only and the other was uniform (Yoongbok) for civilian and militarian. 2. The military uniform (Goonbok) were dressed under armour, was for war time. And the civilian and military uniform were dressed under Mo (Hat) and Po (Coat dress), was for War time or emergent case. 3. Armour were made of leather in ancient times but later they were made of metal. 4. In generally, armour is classified; 1) To protect neck 2) To protect shoulders and arms 3) To protect breast 4) To protect both legs 5) To protect hands 5. Armour and military uniform (Goonbok) for military only. at the time of three Nations (Ko-kuryo, Bakje. Silla) 1) Armour-a) Identified by found relics or ancient wall picture. b) They had improver! armour. c) Armour of three Nations were resembled each other. 2) Military uniform(Goonbok) a) The Jeogori reached to hip area was called Jangyoo. b) The pants were tight trousers. 6. Armour and military uniform (Goonbok) for military only at Koryo Dynasty. 1) They had improved armour like three nation's age. 2) They were made of iron. leather, paper or cloth 3) The color was white. puple. red. 4) Military uniform(Goonbok) a) Hat-(1) Banggak (2) Josamoja (3) Ibgak (4) Jakwan (5) Sabgak (6) Sumale (7) Jaragwan (8) Kummoja (9) Mubyunkwan (10) Pyungyunchek b) Dress-(l) Jayeisokade (2) Bosanghwa Gayendae (3) Hongbeja Rokrahansam (4) Jag- ongbok Hongeung (5) Jagongbok Jogeung (6) Kumyeisokdae (7) Bilapeja Rokrahansam (8) Jasupoto (9) Kumyei Honggung (10) Kumyei Dokuyeunsokdae (11) Bibosunghwa Dongokumdae (12) Bidaesuyei Kayeundae (13) Jasosulansam (14) Biyeiko (15) Chung-yei Dongsim Sokdae 7. Armour and military uniform of Lee Dynasty 1) Armour-a) the Helmets were attached with visor ar without visor, and later it was added the neck protecter. b) dresses were given various names according to the materials used. for example, Suiejakap or Kyungfunkap. 2) The military uniform (Goonbok) were composed by molip, Hyunchungsakpuja, Jundae, Soowhaja. 8. Military uniform (yoongbok) for civilian and militarian 1) The unifom was developped through Imjin war, Byungja war since the middle of Lee Dynasty. 2) The military uniform (Goonbok), (Yoongbok), armour were by the established dress reg-ulation of imperial ordinance dated April 8th, 1895 which falls 35th year of Kojong. the dress regulation was based upon the western uniform.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.2
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• pp.194-199
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• 2011

This study was conducted to evaluate fertigation effects of pig slurry (PS) and chemical fertilizer (CF) in cucumber by investigating the growth and yield, nutrient content and uptake, nutrient use efficiency, and soil characteristics in greenhouse cultivation. The cropping patterns of cucumber were semi-forcing culture and retarding culture, and the experiment involves four treatments: No fertilizer (NF), $26mg\;L^{-1}$ and $52mg\;L^{-1}$ of N application by slurry composting biofiltration liquefied fertilizer (SCBLF), and $52mg\;L^{-1}$ of N treatment by chemical fertilizer. The difference on the plant height of cucumber between SCBLF and CF treatments was no significant, but fresh weight and dry weight of stem and root were higher in $26mg\;L^{-1}$ SCBLF treatment. The Ca content of the leaf was lower in the treatments of SCBLF and the K content of the root was lower in the $52mg\;L^{-1}$ CF treatment. The Ca content of the stem was lowest in the $52mg\;L^{-1}$ CF treatment and the mineral content of the fruit showed no significant difference. In case of semi-forcing culture, the gross yield was lower in the $52mg\;L^{-1}$ CF treatment and the yield of unmarketable fruit was lower in the SCBLF treatments. The nutrient use efficiency of cucumber is as follows: K (8.3 ~ 30.9%), N (4.2 ~ 13.0%), P (1.9 ~ 2.0%). The SCBLF treatments showed higher figure in the soil pH than that of the CF treatment, while EC and the content of $NO_3$-N were higher in the CF treatment than the former. The content of exchangeable K was higher in the plot treated with $52mg\;L^{-1}$ SCBLF, and there were no significant differences in the content of Ca and Mg between the treatments. In conclusion, it is suggested that the application of liquefied manure made from pig slurry may be able to replace the use of chemical fertilizer in nitrogen and potassium.

• Korean Journal of Agricultural Science
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• v.7 no.2
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• pp.65-76
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• 1980

In order to determine the effects of cytokinin and auxin on organ formation from tissue of garlic cloves, leaf blades and basal tissues contained meristem of garlic (Allium sativum L.) cloves harvested in 1979 (old cloves) and 1980 (new cloves) were explanted on a MS medium contained various levels of BA ($N^6$-benzyl amino purine), NAA (naphthalene acetic acid), and 2, 4-D (2, 4-dichlorophenoxyacetic acid). And some of the new cloves were explanted on a media contained BA and NAA after chilling treatment at $4^{\circ}C$ for 10, 20, 30 and 40 days. 1. In a culture of leaf blades of old cloves, shoots were differentiated on a medium supplemented with 2mg/l of BA and NAA. 2. Callus was grown as a quite straw-coloured globular mass on a medium contained 0.2 or 2mg/l 2.4-D. 3. As subcultures of globular calli, shoots and roots were differentiated on a medium contained 2mg/l BA and 0.5 or 1 mg/l NAA, whereas no shoots was shown on a conterol. 4. Shoots were differentiated in a culture of leaf blades of new cloves, but they were not in an old cloves in control, and better effect was shown on a medium contained 2mg/l BA and 1mg/l NAA. However shoots were no differentiated from leaf blades chilled at $4^{\circ}C$ for 30 or 40 days at the same condition. 5. Large numbers of adventitious shoots could be obtained from basal region of garlic cultured on a medium contained 1mg/l BA and 4mg/l NAA, or 2mg/l BA and 2mg/l NAA.

• Journal of Mushroom
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• v.18 no.4
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• pp.357-364
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• 2020

The grade and price of Lentinula edodes largely differs in preference depending on the product area and seasonal factors. The product amount of autumn L. edodes was higher than that of spring L. edodes, but high quality, which is divided into "Hwago" is low in preference. Mostly, the autumn L. edodes is obtained as powder; hence, it is necessary to develop a processing method to utilize its flavor and aroma at an affordable price. Additionally, we estimated the content of 𝛽-glucan, ergosterol, vitamin D₂, reducing sugars, and free amino acids and evaluated the anti-inflammatory activity of saccharification powder of log-cultivated L. edodes. In the saccharification powders obtained via 7 min of UV irradiation of log-cultivated L. edodes, 𝛽-glucan and vitamin D₂ contents were found to be the highest, whereas ergosterol content was found to be the lowest. The content of reducing sugars ranged from 62.4 mg/L to 68.2 mg/L. The free amino acids were higher in these saccharification powders than in the control. Subsequently, RAW 264.7 cells were treated with different concentrations (10, 50, 100, 200, 300, and 500 ㎍/mL) of the saccharification powders of log-cultivated L. edodes obtained via different UV irradiation time applications. The cells showed good viability; the anti-inflammatory effect was found to be the highest at 7 min UV irradiation. Therefore, 7 min of UV irradiation was determined to be the optimum condition for manufacturing saccharification powders of log-cultivated L. edodes. Hence, saccharification powders of log-cultivated L. edodes may be used as a raw material for natural sweeteners, food additives, and in the food industry.

• Korean Journal of Agricultural Science
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• v.36 no.2
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• pp.129-134
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• 2009

Objectives of this study was to find the effect on two different nitrogen levels about agricultural characteristics of CNU rice lines developed at Crop Genetics and Breeding Laboratory, Chungnam Nat'l Univ.. Materials used were two black and one white glutinous rice lines, which were transplanted by hand with $30{\times}15cm$ at CNU paddy field on May 23, 2009. These lines was designed with two replication by two nitrogen levels as 7kg and 9kg per 10a. As the result; Culm length of all lines used were higher at 7kg than 9kg per 10a while panicle length was longer at 9kg nitrogen level. Tillers per plant of CNU 08-01 and CNU 08-02 lines except CNU 08-101 showed highly at 9kg level, but that of other leading varieties were not constant trends in spite of increasing nitrogen. Ripening ratio of CNU 08-01 and 08-02 lines were higher at 7kg but CNU 08-101 line was higher at 9kg. 1,000 grain weight and unhulled rice yield per 20 plants in all CNU lines were highly appeared at 9kg levels per 10a.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.44 no.9
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• pp.54-62
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• 2007

In this paper, a novel wideband notched compact UWB antenna is designed to satisfy the licensed UWB frequency bandwidth($3.1{\sim}4.8$ GHz, $7.1{\sim}10.2$ GHz) by symmetrically arranging two adjacent sectorial loop antennas. The wideband($4.8{\sim}7.1$ GHz) notch can be obtained by inserting the inverted-L shaped slits on the patch. The designed UWB antenna has return loss lower than -10dB at 3.1 GHz and over, group delay value lower than 1 ns and the linear phase property. The optimized UWB antenna inserted the inverted-L shaped slits has return loss great than -10dB, 5 ns of group delay, nonlinear phase and decreased gain properties over the frequency band, 4.8 GHz to 7.1 GHz.

• KSBB Journal
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• v.14 no.5
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• pp.539-542
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• 1999

For the mass production of DFAIII and for the development of techniques of separation and purification of it, the methods of production of DFAIII and its recovery was investigated by fermentation with the strain of Arthrobacter ureafaciens KCTC 3387 and by enzyme reaction. In the first method, DFAIII was produced by fermentation with the strain of Arthrobacter ureafaciens KCTC 3387 and recovered from culture supernatant with silica gel gy filtration, in the second method, it was produced by enzyme reaction and recoverd with the same method of the first, and in third method it was produced by fermentation and recovered by addition of ethanol to the culture supernatnat.Against 25g/L of initial concentration of inulin, 1.57, 4.40, 0.34 g/L of powder of DFAIII was recovered respectively and the rate of recovery was 6.3, 17.6 1.4% and the purity was estimated at 81, 97, 87% respectively. For the production of DFAIII and its recovery, enzyme reaction method was the highest in the rate of recovery and its purity. By fermentation method, DFAIII was produced with 50% fo initial concentration of substrate but th rate of recovery was lower than enzyme reaction method and purity was lowest among the three methods. Ethanol pricipitation method showed the lowest rate of recovery.

• Journal of Sericultural and Entomological Science
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• v.47 no.1
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• pp.31-35
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• 2005

This study had done to find an easily-dyeing method for novices because the dyeing method of traditional indigo has not been general to the public so that only an expert could dye the fibers. The results are as the following. When the powders after burning the shell of cockle (tegillarca granosa) were added. pH of the solution was 12.35 The k/s value of 2.49 was the highest in the dye after dipping in the solution of indigo for 1-2 days and the k/s value was 3.10 when adding 20 g/l of the starch (55% corn-starchy products in Korean market) into the solution of indigo. In addtion, the k/s value was the highest when fermenting temperature was $30^{\circ}C$ and when the powders after burning the cockle shell were 4 g/l. There were no differences between water and lye of rice straw which had used for the ferment of indigo. The components of two dye which has traditionally made of the cockle shell and which has made of calcium hydroxide were all the same.

• Journal of Pharmaceutical Investigation
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• v.35 no.6
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• pp.437-443
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• 2005

A rapid, selective and sensitive reversed-phase HPLC method for the determination of a major metabolite of terfenadine, fexofenadine, in human serum was developed, validated, and applied to the pharmacokinetic study of terfenadine. Fexofenadine and internal standard, haloperidol were extracted from human serum by liquid-liquid extraction with acetonitrile and analyzed on a $Symmetry^{TM}$ C8 column with the mobile phase of 1% triethylamine phosphate (pH 3.7)-acetonitrile (67:33, v/v, adjusted to pH 5.6 with triethylamine). Detection wavelength of 230 nm for excitation, 280 nm for emission and flow rate of 1.0 mL/min were fixed for the study. The assay robustness for the changes of mobile phase pH, organic solvent content, and flow rate was confirmed by $3^{3}$ factorial design using a fixed fexofenadine concentration (50 ng/mL) with respect to its peak area and retention time. In addition, the ruggedness of this method was investigated at three different laboratories using same quality control (QC) samples. This method showed linear response over the concentration range of 10-500 ng/mL with correlation coefficients greater than 0.999. The lower limit of quantification using 0.5 mL of serum was 10 ng/mL, which was sensitive enough for the pharmacokinetic studies of terfenadine. The overall accuracy of the quality control samples ranged from 95.70 to 114.58% for fexofenadine with overall precision (% C.V.) being 3.53-14.39%. The relative mean recovery of fexofenadine for human serum was 90.17%. Stability studies (freeze-thaw, short-term, extracted serum sample and stock solution) showed that fexofenadine was stable during storage, or during the assay procedure in human serum. However, the storage at $-70^{\circ}C$ for 4 weeks showed that fexofenadine was not stable. The peak area and retention time of fexofenadine were not significantly affected by the changes of mobile phase pH, organic solvent content, and flow rate under the conditions studied. This method showed good ruggedness (within 15% C.V.) and was successfully used for the analysis of fexofenadine in human serum samples for the pharmacokinetic studies of orally administered Tafedine tablet (60 mg as terfenadine) at three different laboratories, demonstrating the suitability of the method.