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Exo-Polysaccharide Production in Liquid Culture of Pleurotus ferulae

  • CHOI DU BOK;KANG SI HYUNG;SONG YON HO;KWUN KYU HYUK;JEONG KYOUNG JU;CHA WOL SUK
    • Journal of Microbiology and Biotechnology
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    • v.15 no.2
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    • pp.368-375
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    • 2005
  • Batch cultures were carried out to optimize the exo-polysaccharide production by liquid cultures of Pleurotus ferulae. Among the various carbon sources, when $5\%$ of glucose was used, the maximum mycelial growth and exo-polysaccharide concentration reached were 8.78 g/l and 3.59 g/l, respectively. Yeast extract and polypeptone were identified as the most suitable nitrogen sources. In particular, when a mixture of $1\%$ of polypeptone and $0.8\%$ of yeast extract was used, 9.52 g/l of mycelial growth and 4.09 g/l of exo-polysaccharide were obtained. In the case of mineral sources, K$_2$HPO$_4$ and MgSO$_4$$\codt$7H$_2$0 were found to be the best mineral sources for mycelial growth and exo-polysaccharide production. Under the optimized culture conditions, the agitation speed and aeration were investigated for mycelial growth and exo­polysaccharide production in a jar fermentor. The maximum mycelial growth and exo-polysaccharide concentration at 1.5 vvm and 200 rpm obtained were 13.2 g/l and 4.95 g/l, respectively, after 10 days of culture, which were $76\%$ and $79\%$ higher than those of the basal medium. The specific growth rate was decreased with the increase of mycelial growth. However, the specific production rate of the exo-polysaccharide was proportionally increased with the specific growth rate. The proposed model profiles showed good agreement with the experimental results for the mycelial growth and exo-polysaccharide production. The specific production rate using the optimized medium was higher than that of basal medium.

Experimental study of Nutrient Removal by Endogeneous Nitrate Respiration (ENR) Mechanism in domestic wastewater (질산성질소의 내생탈질기작을 이용한 하수내 영양소 제거에 관한 실험적 연구)

  • Park, Myung-Gyun;Ahn, Won-Sik;Lee, Eui-Sin;Heo, Yong-Rok;Park, Chong-Bok
    • Clean Technology
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    • v.8 no.2
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    • pp.77-83
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    • 2002
  • The purpose of this study is to develop the efficient nutrient removal process and to verify operation and design parameters in domestic sewage. Endogenous nitrate respiration (ENR) was used for denitrification of nitrate in return sludge without additional organic carbon source. ENR reactor before the anaerobic tank enable to reduce nitrate below 3 mg/L and increase phosphate release at anaerobic reaction. Primary effluent during pilot scale plant were shown as TCOD/TP ratio of 40~60 and TCOD/TKN ratio of 5~7. Effluent concentrations were 10 to 12mg/L as TN and 1mg/L as TP respectively. In lab scale experiments endogenous denitrification rate of ENR reactor ranges from 0.042 to $0.057gNO_3-N/gMv.d.$ $SP_{rel}/SCOD_{rm}$ was shown as from 0.13 to 0.17 in anaerobic reaction. These kinetic parameters are expected to be available for BNR(Biological Nutrient Removal) plant design and ENR reaction is available for nutrient removal in low strength wastewater.

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Evaluation of the Inhibitory Effect of Cu2+, Al3+ and Zn2+ on the Activated Sludge (Cu2+, Al3+, Zn2+이 활성슬러지에 미치는 저해영향 평가)

  • Kim, Chang-Gyu;Kim, Yo-Yong;Park, Ik-Beom;Song, Jin-Ho;Nam, Woo-Kyong;Han, Song-Hee;Kim, Bok-Jun;Oh, Jo-Gyo
    • Journal of Korean Society on Water Environment
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    • v.31 no.2
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    • pp.87-93
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    • 2015
  • In this study, the inhibitory effect of toxic metals was investigated on the activated sludge of the municipal sewage treatment plant. The allowable concentration of toxic metals was also estimated for the stable operation of the biological treatment process. The single and mixture toxicity of $Cu^{2+}$, $Al^{3+}$ and $Zn^{2+}$ were evaluated for the activated sludge microorganisms. As a result, nitrifying microorganisms were more susceptible than heterotrophic microorganisms. $IC_{10}$ (Inhibition Concentration of 10%) of $Cu^{2+}$, $Al^{3+}$ and $Zn^{2+}$ for the nitrifying microorganisms was 3 mg/L, 7 mg/L and 25 mg/L, respectively. The mixture toxicity showed three times more sensitive than the single toxicity. The concentrations of $Cu^{2+}$, $Al^{3+}$ and $Zn^{2+}$ to minimize the inhibitory effect on organic matter removal and nitrification in batch experiments were found to be 1.3 mg/L, 2.5 mg/L and 6.3 mg/L.

Distribution characteristic of invasive alien plants in Jeju Island

  • Ryu, Tae-Bok;Kim, Mi-Jeoung;Lee, Chang-Woo;Kim, Deok-Ki;Choi, Dong-Hui;Lee, Hyohyemi;Jeong, Hye-Ran;Lee, Do-Hun;Kim, Nam-Young
    • Journal of Ecology and Environment
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    • v.41 no.6
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    • pp.173-181
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    • 2017
  • Background: This study was undertaken to analyze the distribution and ecological characteristics of invasive alien plant species on Jeju Island, and to provide basic data for their management and control. Results: A field research was conducted at 436 locations on Jeju Island. The field research identified nine species of invasive alien species growing on Jeju Island. Based on the distribution pattern, Hypochaeris radicata L., Rumex acetosella, and Ambrosia artemisiifolia L. were found to be distributed horizontally throughout Jeju Island, with vertical growth in two or more vegetation zones, from warm temperate to the subalpine zone. Widely distributed species penetrate various habitats, such as grasslands, ranches, roadsides, farmlands, and empty lots, and have an immensely negative impact on the ecosystem, including declining biodiversity on Jeju Island. Paspalum distichum var. indutum Shinners, Paspalum distichum L., Solanum carolinense L., and Aster pilosus Willd. were distributed in some areas as a biased distribution species, whereas Lactuca scariola L. and Solidago altissima L. were found only in certain areas as centralized distribution species. Conclusions: The centralized distribution species and biased distribution species of the invasive plants in the ecosystem of Jeju Island should be physically eliminated, keeping in mind the short- and mid-term perspectives and monitoring, and by considering expansion of additional distribution areas. Due to limitations of physical/chemical elimination, time, and cost, widely distributed species require to be eliminated and managed, mainly to restore the integrity of the ecosystem, by planting native species to reestablish the habitat.

Weed Control Technology with Low Concentration Ethanol in Protected Cereal Crop Cultivation (비닐하우스 내 조 재배시 저농도 에탄올을 이용한 잡초억제 효과)

  • Hwang, Jae-Bok;Yun, Eul-Soo;Park, Chang-Young;Jung, Ki-Youl;Choi, Young-Dae;Jeon, Sung-Ho;Lee, Yong-Hwan
    • Korean Journal of Weed Science
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    • v.32 no.2
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    • pp.115-120
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    • 2012
  • Weed control is of fundamental importance when planting cereal crops, particularly during the establishment phase. Weeds compete for nutrients, water and light, and can severely threaten the survival and early growth of newly planted crops. Field experiment was conducted to evaluate the effect of ethanol treatment on the weed control efficacy in cereal crops in protected vinylhouse. Five treatments like water, 25 L, 50 L, 75 L, and 100 L per $m^{-2}$ at 2% ethanol were applied and covered with vinyl on 10 days before foxtail millet seeding. After that, foxtail millet seeds were sown on June 20 at $60{\times}10cm$ planting distance and weed control efficacy was recorded at 30, and 50 days after seeding based on the dry weight. Soil oxidation-reduction potential was recorded -200~-400 mV after treatment 2 days. Weed control efficacy of at 75 L, and 100 L per $m^{-2}$ was observed 92%, 84% in 2% ETOH, respectively. Yields of foxtail millet at 75 L, and 100 L per $m^{-2}$ were 1,570 kg $ha^{-1}$, 1,230 kg $ha^{-1}$, respectively.

An Assessment of Air Sampling Location for Stack Monitoring in Nuclear Facility (원자력시설 굴뚝 내 공기시료채취 위치의 적절성 평가)

  • Lee, JungBok;Kim, TaeHyoung;Lee, JongIl;Kim, BongHwan
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.15 no.2
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    • pp.173-180
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    • 2017
  • In this study, air sampling locations in the stack of the Advanced Fuel Science Building (AFSB) at the Korea Atomic Energy Research Institute (KAERI) were assessed according to the ANSI/HPS N13.1-1999 specification. The velocity profile, flow angle and $10{\mu}m$ aerosol particle profile at the cross-section as functions of stack height L and stack diameter D (L/D) were assessed according to the sampling location criteria using COMSOL. The criteria for the velocity profile were found to be met at 5 L/D or more for the height, and the criteria for the average flow angle were met at all locations through this assessment. The criteria for the particle profile were met at 5 L/D and 9 L/D. However, the particle profile at the cross-section of each sampling location was found to be non-uniform. In order to establish uniformity of the particle profile, a static mixer and a perimeter ring were modeled, after which the degrees of effectiveness of these components were compared. Modeling using the static mixer indicated that the sampling locations that met the criteria for the particle profile were 5-10 L/D. When modeling using the perimeter ring, the sampling locations that met the criteria for particle profile were 5 L/D and 7-10 L/D. The criteria for the velocity profile and the average flow angle were also met at the sampling locations that met the criteria for the particle profile. The methodologies used in this study can also be applied during assessments of air sampling locations when monitoring stacks at new nuclear facilities as well as existing nuclear facilities.

Integer Factorization for Decryption (암호해독을 위한 소인수분해)

  • Lee, Sang-Un;Choi, Myeong-Bok
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.13 no.6
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    • pp.221-228
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    • 2013
  • It is impossible directly to find a prime number p,q of a large semiprime n = pq using Trial Division method. So the most of the factorization algorithms use the indirection method which finds a prime number of p = GCD(a-b, n), q=GCD(a+b, n); get with a congruence of squares of $a^2{\equiv}b^2$ (mod n). It is just known the fact which the area that selects p and q about n=pq is between $10{\cdots}00$ < p < $\sqrt{n}$ and $\sqrt{n}$ < q < $99{\cdots}9$ based on $\sqrt{n}$ in the range, [$10{\cdots}01$, $99{\cdots}9$] of $l(p)=l(q)=l(\sqrt{n})=0.5l(n)$. This paper proposes the method that reduces the range of p using information obtained from n. The proposed method uses the method that sets to $p_{min}=n_{LR}$, $q_{min}=n_{RL}$; divide into $n=n_{LR}+n_{RL}$, $l(n_{LR})=l(n_{RL})=l(\sqrt{n})$. The proposed method is more effective from minimum 17.79% to maxmimum 90.17% than the method that reduces using $\sqrt{n}$ information.

Introduction of two-step culture method for multiple seed bulb development from shoot tip culture of garlic (Allium sativium L.) (마늘의 경정배양에서 기내인경구 대량생산을 위한 2단계 배양의 도입)

  • Hwang, Hye-Yeon;Lee, Young-Bok
    • Journal of Plant Biotechnology
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    • v.35 no.1
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    • pp.75-80
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    • 2008
  • In vitro culture of shoot tip of garlic (Allium sativium L. cv. Seosan) was carried out to find medium condition of the induction of multiple shoots and bulbing for muliproduction of virus-free seed bulbs. For this work, tank culture was introduced. In shoot tip culture on MS solid medium the induction of multiple shoots and bulbing were better by adding 3% sucrose than 8%. Supplementation with 2mg/L 2ip and 0.2 mg/L IAA in this medium was effective. Three point three shoots including 2.7 bulbs were formed from a shoot tip after cultivation for 30 days on this medium. Bulbing of garlic in liquid culture with plastic water tank of 20L supplied air at the side of the lower part was better by adding 3% sucrose than 8% by subculture for 45 days with shoots obtained from shoot tip culture for 30 days on soid MS medium. Shoot growth was vigorous at 3% sucrose however bulb growth was more effective on the medium of 8% sucrose. Because of the effectiveness on solid medium added 3% sucrose, 2 mg/L 2ip and 0.2 mg/L IAA for initial production of multi-shoot in stem tip culture and the effectiveness in liquid culture with water tank for growth of bulbs, the method of two-step culture could be introduced for the multiple production of seed bulb of high quality. It was more desirable by supply of 0.2 mg/L BA and 0.02 mg/L NAA at tank culture time. But growth of the bulbs became poor by increasing concentration of NAA of the medium.

Inactivation of Escherichia coli, Saccharomyces cerevisiae, and Lactobacillus brevis in Low-fat Milk by Pulsed Electric Field Treatment: A Pilot-scale Study

  • Lee, Gun Joon;Han, Bok Kung;Choi, Hyuk Joon;Kang, Shin Ho;Baick, Seung Chun;Lee, Dong-Un
    • Food Science of Animal Resources
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    • v.35 no.6
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    • pp.800-806
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    • 2015
  • We investigated the effects of a pulsed electric field (PEF) treatment on microbial inactivation and the physical properties of low-fat milk. Milk inoculated with Escherichia coli, Saccharomyces cerevisiae, or Lactobacillus brevis was supplied to a pilot-scale PEF treatment system at a flow rate of 30 L/h. Pulses with an electric field strength of 10 kV/cm and a pulse width of 30 µs were applied to the milk with total pulse energies of 50-250 kJ/L achieved by varying the pulse frequency. The inactivation curves of the test microorganisms were biphasic with an initial lag phase (or shoulder) followed by a phase of rapid inactivation. PEF treatments with a total pulse energy of 200 kJ/L resulted in a 4.5-log reduction in E. coli, a 4.4-log reduction in L. brevis, and a 6.0-log reduction in S. cerevisiae. Total pulse energies of 200 and 250 kJ/L resulted in greater than 5-log reductions in microbial counts in stored PEF-treated milk, and the growth of surviving microorganisms was slow during storage for 15 d at 4℃. PEF treatment did not change milk physical properties such as pH, color, or particle-size distribution (p<0.05). These results indicate that a relatively low electric-field strength of 10 kV/cm can be used to pasteurize low-fat milk.

Proximate Composition, and ʟ-Carnitine and Betaine Contents in Meat from Korean Indigenous Chicken

  • Jung, Samooel;Bae, Young Sik;Yong, Hae In;Lee, Hyun Jung;Seo, Dong Won;Park, Hee Bok;Lee, Jun Heon;Jo, Cheorun
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.12
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    • pp.1760-1766
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    • 2015
  • This study investigated the proximate composition and $\small{L}$-carnitine and betaine content of meats from 5 lines of Korean indigenous chicken (KIC) for developing highly nutritious meat breeds with health benefits from the bioactive compounds such as $\small{L}$-carnitine and betaine in meat. In addition, the relevance of gender (male and female) and meat type (breast and thigh meat) was examined. A total of 595 F1 progeny (black [B], grey-brown [G], red-brown [R], white [W], and yellow-brown [Y]) from 70 full-sib families were used. The moisture, protein, fat, and ash contents of the meats were significantly affected by line, gender, and meat type (p<0.05). The males in line G and females in line B showed the highest protein and the lowest fat content of the meats. $\small{L}$-carnitine and betaine content showed effects of meat type, line, and gender (p<0.05). The highest $\small{L}$-carnitine content was found in breast and thigh meats from line Y in both genders. The breast meat from line G and the thigh meat from line R had the highest betaine content in males. The female breast and thigh meats showed the highest betaine content in line R. These data could be valuable for establishing selection strategies for developing highly nutritious chicken meat breeds in Korea.