• Asian-Australasian Journal of Animal Sciences
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• 제22권9호
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• pp.1248-1255
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• 2009

This study was carried out to evaluate the fatty acid composition in Nellore cows supplemented with either linseed (n-3) or canola grains (n-6 and n-9). Fifteen Nellore cows, aged five years and bodyweight 550 kg${\pm}$48 kg, were randomly distributed to the following treatments: CON (control), LIN (linseed) and CAN (canola grains). The cows were fed for 80 days. The concentrations of C18:0, C18:2 n-6 and C20:3 n-6 fatty acid were higher (p<0.10) in CON blood plasma in comparison to follicular liquid. Likewise, PUFA, n-6 contents, PUFA:SFA and n-6:n-3 ratios were higher (p<0.10) in blood plasma. On the other hand, C18:1 n-9, C22:5 n-3, MUFA and n-3 contents were lower (p<0.10) in blood plasma. C18:0, C18:2 n-6, C18:3 n-3, C22:5 n-3, PUFA, n-6, n-3 contents and PUFA:SFA ratio were higher (p<0.10) in LIN blood plasma than in the follicular liquid. Nevertheless, C14:0, C16:0, C16:1 n-7, PUFA, C16:0, C18:1 n-9 and MUFA contents were lower (p<0.10) in LIN blood plasma. On treatment CAN, the C18:0 and SFA contents, and n-6:n-3 ratios were higher (p<0.10) in blood plasma. However, C20:3 n-6, C22:5 n-3, PUFA and n-3 contents were lower (p<0.10) in blood plasma. C16:0, C18:0, PUFA, SFA contents and PUFA:SFA ratio did not differ (p>0.10) among the treatments. C14:0, C16:1 n-7, C18:2 n-6 and n-6 contents were higher (p<0.10) for CON and CAN than LIN. C17:1 n-7, C20:4 n-6 and C 22:0 contents were higher (p<0.10) for CAN than CON and LIN. C18:1 n-9, C18:3 n-3, MUFA and n-3 contents were higher (p<0.10) for LIN and CAN than CON. C20:3 n-6 content and n-6:n-3 ratio were higher (p<0.10) for CON than LIN and CAN. C22:5 n-3 content were higher (p<0.10) for CON and LIN than CAN. The concentrations of fatty acids in blood plasma and follicular liquid were not correlated for any fatty acid, independent of the treatment studied. Canola grain added to the diet of Nellore cows resulted in increased concentrations of fatty acids n-6 and n-3 in follicular liquid.

• Journal of Animal Science and Technology
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• 제59권12호
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• pp.28.1-28.8
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• 2017

Background: Major amino acids in pig diets are Lys, Met, Thr, and Trp, but little is known about the requirements for the other essential amino acids, especially on odorous compounds and microbial characteristics in feces of growing-finishing pigs. To this end, different levels of amino acid composition added to diets to investigate the effects of amino acid composition on microbial characteristics and odorous compounds concentration. Methods: A total eight (n = 8) barrows (Landrace ${\times}$ Yorkshire ${\times}$ Duroc) with an average bodyweight of $89.38{\pm}3.3kg$ were individually fed diets formulated by Korean Feeding Standards 2007 (old version) or 2012 (updated with ideal protein concept) in metabolism crates with two replication. After 15-day adaptation period, fresh faecal samples were collected directly from pigs every week for 4 weeks and analysed for total volatile fatty acids (VFA), phenols and indoles by using gas chromatography. The nitrogen was determined by Kjeldahl method. Bacterial communities were detected by using a 454 FLX titanium pyrosequencing system. Results: Level of VFA tended to be greater in 2012 than 2007 group. Among VFAs, 2012 group had greater (p < 0.05) level of short chain fatty acids (SCFA) than control.Concentration of odorous compounds in feces was also affected by amino acid composition in pig diet. Levels of ammonium and indoles tended to be higher in 2012 group when compared with 2007 group.Concentration of phenols, p-cresol, biochemical oxygen demand, and total Kjeldahl nitrogen, however, were lower (P < 0.05) in 2012 treatment group compare to 2007. The proportion of Firmicute phylum were decreased, while the Bacteriodetes phylum proportion increased and bacterial genera includingCoprococcus, Bacillus, and Bacteroides increased (p < 0.05) in 2012 compare to 2007 group. Conclusion: Results from our current study indicates that well balanced amino acid composition reduces odor by modulating the gut microbial community. Administration of pig diet formulated with the ideal protein concept may help improve gut fermentation as well as reduce the odor causing compounds in pig manure.

• 농업과학연구
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• 제44권4호
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• pp.531-540
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• 2017

A study was conducted to compare growth performance of six female commercial Korean native chicken (KNC) crossbreeds from hatching to twelve weeks of age. Three hundred and twelve, 1-day-old female commercial KNC were used within 1 paternal line and 6 maternal lines. The chickens were allocated to 24 battery cages to give 4 replicates per strain with 13 chickens per cage. The chickens were reared under continuous lighting (24 h) and water was available at all times. Ad-libitum feeding was practiced throughout the experimental period. Among the six different strains, 2A had the greatest bodyweight (BW) at 42 days after hatching (p < 0.05). No BW difference between six crossbreed strains (p > 0.05) was found thereafter. Crossbreed 1A had the higher average daily gain (ADG) than crossbreed 2A and 3A chickens (p < 0.05), whereas crossbreed 4A, 5A, and 6A had similar ADGs to that of crossbreed 1A (p > 0.05) at 84 days after hatching. Furthermore, crossbreed 4A had a great average daily feed intake (ADFI) from hatching to 84 days (p < 0.05). Nonetheless, there was no difference in the feed conversion ratio (FCR) and uniformity between six crossbreed strains for the experimental period (p > 0.05). Despite that 1A, 4A, and 6A had the higher viability (p < 0.05) than crossbreed 2A and 5A, they had a similar viability than crossbreed 3A (p > 0.05). With this in mind, crossbreed 2A had greater BW, ADG, and FCR than other chicken crossbreeds from hatching to 84 days, although they had a lower viability than others.

• Asian-Australasian Journal of Animal Sciences
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• 제15권3호
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• pp.390-395
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• 2002

Two experiments were conducted to study the performance and carcass characteristics of Hanwoo (Korean native beef cattle) steers (Experiment 1) and the nutrient digestibility of sheep (Experiment 2) when the animals fed diets containing four levels of dried wormwood (Artemisia sp.). For both experiments the animals were given a basal diet consisting of rice straw and concentrate mixed at 3:7 ratio (on DM basis). In Experiment 1, the treatments were designed as a completely randomized design with two feeding periods. Steers were allotted in one of four dietary treatments, which were designed to progressively substitute dried wormwood for 0, 3, 5 and 10% of the rice straw in the basal diet. Bodyweight gain and average daily gain (ADG) of Hanwoo steers fed diets containing the 5 and 10% wormwood inclusion were greater (p<0.05) than the 3% wormwood-feeding group. Total and daily intakes of roughage and concentrate were not altered by all levels of dried wormwood inclusion. However, the 10% dried wormwood inclusion led to increased total feed intake (p<0.05) compared with that of the 0 and 3% of dried wormwood inclusion. Carcass weight, carcass yield and backfat thickness were not altered by dried wormwood inclusion. The highest level of dried wormwood inclusion resulted in significantly increased (p<0.05) loin-eye area compared with the 0 and 5% levels of dried wormwood inclusion. Experiment 2 was designed by a $4{\times}4$ Latin square with four periods. Sheep were allocated in one of four dietary treatments as same as in Experiment 1. Digestibilities of DM and TDN were significantly increased (p<0.05) in sheep fed the diet containing all three levels of dried wormwood inclusion compared with the control treatment. Digestibilities of CP and crude fiber in the 5% dried wormwood inclusion highly increased (p<0.05) up to 8.2 and 5.5% respectively relative to the control treatment. The palatability was significantly improved (p<0.05) by the 5% dried wormwood inclusion compared with the control treatment. It is concluded that feeding diets containing dried wormwood substituted for equal weights of rice straw at 5 and 10% levels would be anticipated to provide better quality roughage for beef cattle production and higher income for beef cattle producers.

• 한국산학기술학회논문지
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• 제21권10호
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• pp.231-239
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• 2020

본 연구의 목적은 TCDD로 인해 발생하는 산화적 스트레스에 대한 GYB의 내분비 기능 및 항산화 효능에 미치는 영향을 평가하기 위한 것이다. 2017년도에, 산화적 스트레스에 대한 GYB의 효능을 평가하기 위해 35마리의 수컷 SD랫드를 5개의 그룹으로 나누어 실험하였다. 정상 대조군은 vehicle 및 saline을 투여하였으며, TCDD 단독투여그룹은 TCDD(2 ㎍/kg, weeks) 및 생리식염수를 복강 내 투여하였으며, 시험 군은 6주 동안 GYB (75, 150, 300 mg/kg)를 3가지 농도로 나누어 경구로 투여하였다. 체중은 NC 그룹의 랫드와 비교하였을 때 TCDD 노출 후 6주 후에 유의하게 감소하였다(p<0.001). 그러나 TCDD에 의한 체중 감소는 GYB 300 mg/kg 투여에 의해 현저하게 보호되었다(p<0.01). TCDD로 유도된 랫드의 간은 세포질의 공포 변성이 나타났으며, 간의 동양 혈관과 중량의 증가를 나타냈다. MDA, SOD를 측정한 결과, TCDD 투여에 의하여 두 항목 모두 감소하는 경향이 나타났다. 반면, GYB 투여에 의한 변화는 나타나지 않았고, SOD 결과에서 GYB 300군에서 NC군 대비 유의성이 관찰(p<0.05) 되었다. 이러한 발견은 GYB가 랫드에서 TCDD 유도 간 독성에 대해 보호 효과를 가질 수 있음을 증명하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권2호
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• pp.189-197
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• 2006

Twenty small-scale farms of two villages (A and B) were surveyed to identify the feeding traits, milk productivity and nutritional status of lactating cattle and buffalo in Terai, Nepal. Constituents and dry matter (DM) of feed supplied, body condition score (BCS), heart girth (HG), bodyweight (BW), milk yield (MY) and plasma metabolites were obtained in the pasture-sufficient, pasture-decreasing and fodder-shortage periods. Milk yield of 305-day lactation was estimated by the daily MY. The supplies of rice straw and native grass were lower and higher in the pasture-sufficient period than in the other periods, respectively (5.5 kg/day vs. 9.8 kg/day and 3.2 kg/day vs. 0.4 kg/day, respectively, p<0.01). The roughage-supplement rates of the animals were higher in village A than in village B (5.0 vs. 2.2 in cattle and 9.3 vs. 1.8 in buffalo, p<0.01). The variance of feed constituents among the periods and between the villages induced different supplies of CP, NDF and TDN. The concentrations of CP and TDN in the cattle feed were higher in the pasture-sufficient period than in the other periods (9.1% vs. 7.3% and 57.4% vs. 51.0%, respectively, p<0.01). The supplies of CP for cattle and buffalo, and of TDN for buffalo were lower in village A than in village B (7.5% vs. 8.7% and 6.6% vs. 9.1% [p<0.01], and 53.1% vs. 56.2% [p<0.05], respectively). The BCS, HG and BW of the animals were lower in village A than in village B (2.51 vs. 2.86, 156 cm vs. 170 cm and 300 kg vs. 318 kg, respectively in cattle, 2.83 vs. 4.00, 186 cm vs. 216 cm and 429 kg vs. 531 kg, respectively in buffalo, p<0.01). The cattle yielded more milk in the pasture-sufficient period than in the other periods (7.9 liters/day vs. 6.6 liters/day, p<0.01). The 305-day MY of cattle that calved in the fodder-shortage period was lower than that of cattle that calved in the other periods (1,900 liters vs. 2,251 liters, p<0.01). The MYs of cattle and buffalo were lower in village A than in village B (6.2 liters/day vs. 8.1 liters/day and 3.7 liters/day vs. 7.7 liters/day, respectively, p<0.01). The 305-day MY of cattle was lower in village A than in village B (1,935 liters vs. 2,409 liters, p<0.01). The concentrations of plasma albumin and urea nitrogen in cattle were lower in village A than in village B (3.2 g/dl vs. 3.4 g/dl [p<0.01] and 7.4 mg/dl vs. 10.2 mg/dl [p<0.05], respectively). The different supplies of CP, NDF and TDN among the periods and between the villages might have affected MY and nutritional status in cattle and buffalo. It was likely that the lower supplies of CP and TDN for cattle that calved in the fodder-shortage period and in village A lowered the 305-day MY of cattle.

• 한국동물위생학회지
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• 제29권1호
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• pp.55-63
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• 2006

Alcoholism and alcohol abuse are major public health concerns. This is linked to the injury of many organs, especially liver. Experiments were peformed to know the acute effects of LeeKwaDoo (LKD) induced by two-third partial hepatectomy (PH) in rats. In liver samples, regeneration parameters and histological assessment were performed. For the blood biochemical study, the blood were assayed with AST, ALT. The portal branch of liver lobes was ligated in the male Sprague-Dowley rats, two-thirds partial hepatectomies were also performed. It was estimated bodyweight and relative liver weight for the index of liver mass. For the marker of blood chemistry, we investigate the serum sample of rats and demonstrated the level of AST, ALT. Remaining tissues of liver developed as microscopic structures. Resection of the lobes in PH+LKD group resulted in a marked change of liver weight, blood chemistry and histological changes. The initiation of the proliferative response in PH group stimulated as well as reduction of the liver mass. On the other hands, the Initiation of the proliferative response in PH+LKD group delayed. Eventually, both PH group and PH+LKD group was restored relative liver weigh after 7 day. In conclusion, the acute adminstration of LKD seems to inhibit the initial response of liver regeneration through alcohol effects.

• 한국임상수의학회지
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• 제29권2호
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• pp.130-133
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• 2012

동적 컴퓨터단층촬영을 이용하여 연령에 따른 CT-GFR 수치에 대한 패턴을 살펴보기 위해 본 연구를 실시하였다. 품종이나 성별에 대한 제한 없이 다양한 연령으로 15마리의 개를 이용하였다. 혈액검사, 요검사, 영상학적 검사를 실시하여 개의 건강 상태를 확인한 후, propofol-isoflurane을 이용한 호흡마취를 실시하여 CT 촬영을 하였다. Iohexol (1 ml/kg, 300 mgI/ml)을 3 ml/s 일정한 속도로 2분간 주입하면서 1.5초 간격으로 총 80개의 영상을 촬영하여 파트락 분석을 통해 사구체여과율 (ml/min/kg)을 측정하였다. 양측 신장의 사구체여과율의 범위는 2.57-6.60 ml/min/kg이었다. 연구에서 연령에 따라 사구체여과율의 유의적인 차이는 확인되지 않았다.

• 대한본초학회지
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• 제30권6호
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• pp.63-68
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• 2015

Objectives : Gastric lesions affect many people around the world and their development are results of the imbalance between destructive and protective factors in the gastric mucosa. Lycium chinense has been widely used as a traditional Korean medicine, it was recently reported that they have potent anti-inflammatory effects in chronic hepatitis models. Therefore, this study aimed to investigate the anti-inflammatory activity of Lycium chinense extract (LCE) on HCl-Ethanol induced gastric lesion mice.Methods : The ICR mice were divided randomly into five groups of six animals each. Group A was normal mice, and group B was treated orally with 0.5 ml 150 mM HCl-60% Ethanol. Mice in group C and D were pre-treatment of LCE (100 mg/kg and 200 mg/kg bodyweight, p.o before HCl/ethanol treatment) and group E was orally administered sucralfate (10 mg/kg).Results : 150mM HCl/60% ethanol-induced gastric mucosal injury mice were ameliorated mucosal damage upon histological evaluation by treatment of LCE. Pre-treatment of LCE attenuated reactive oxidative species (ROS) and produces peroxynitrite (ONOO^-) in stomach tissues. As results of stomach protein analyses, LCE effectively reduce inflammatory-related factors such as cyclooxygenase-2 (COX-2), tumor necrosis factor alpha (TNF-α), inducible nitric oxide synthase (iNOS) and interleukin-6 (IL-6) in gastric lesion mice. In addition, nuclear factor kappa B (NF-κB) and inhibitor of phosphorylation of nuclear factor kappa B (p-IκB) were down-regulated in LCE-administrated gastric lesion mice.Conclusions : Our discovery supports that the therapeutic activity of LCE ameliorate the development of gastric lesion via suppressing the oxidative stress and gastric partial inflammation induced by 150 mM HCl/60% ethanol.

• 농업과학연구
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• 제47권3호
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• pp.605-614
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• 2020

This study was conducted to compare the growth performances of three groups of commercial Korean native chickens (KNCs) including two strains of crossbreeds and H3 (Hanhyeop 3) from hatch to twelve weeks of age. (1A, 2A, and H3). A total of 468 one-day-old chicks were allocated in a completely randomized design with 15 replicates per treatment for the crossbreeds and 9 replicates per treatment for H3 (12 birds per cage). Commercial broiler diets (i.e., Week 0 - 5 crude protein [CP] 22.0%, metabolizable energy [ME] 3,025 kcal·kg^-1; week 5 - 8 CP 20.0%, ME 3,100 kcal·kg^-1; week 8 - 12 CP 19.0%, ME 3,150 kcal·kg^-1) were provided according to the Korean Feeding Standard for Poultry on an ad-libitum basis with fresh clean water during the twelve-week period. Body weight gain and shank length (SL) were measured weekly until week 6 and bi-weekly during week 6 to 12. Compared to H3, the two crossbreed groups had a higher body weight (BW) on weeks 3 to 8; however, the bodyweight of H3 on week 10 was significantly higher than the other groups (p < 0.05). Moreover, the average daily feed intake (ADFI) of H3 was higher than that of the two crossbreed groups from hatching to 84 days except for week 3, and H3 showed a lower average daily gain (ADG) on weeks 3 and 10 (p < 0.05). Furthermore, H3 had a higher feed conversion ratio compared to another crossbreed chicken on weeks 1 to 8 and the last week after hatching. Among all the groups, there was no significant difference for shank length during the experimental period.