Two sets of data on carcass traits and beef cut parameters were used to investigate the relationships between carcass and beef cut measurements, which can be used to make predictions of retail cut percentages. One set had a total of 1,141 measurements of Hanwoo cattle of three different sex origins, which were slaughtered in an abattoir located at the National Institute of Animal Science, RDA, Korea from 1996 to 2008. To develop prediction models for retail cut percentage with higher accuracies than the current model, another set consisting of a total of 13,389 records of carcass and beef cut traits were collected from 30 abattoirs and butcheries in Korea from 2008 to 2009. Bulls yielded heavier and leaner carcasses than steers. High correlation coefficients were estimated between amount of body fat and percent retail cut (-0.82) as well as between back fat thickness (BF) and percent retail cut (-0.62). The amount of retail cut, however, was highly correlated with body weight before slaughter (BW, 0.95) or with cold carcass weight (CWT, 0.94). Relationships between percent retail cut and measurable beef yield traits, BF, loin eye area (LEA) or CWT varied by sex class, which must be considered for development of a prediction model with high accuracy. Models of data for all breeds and sexes fit the effects of breed, sex, and interaction of abattoir by butchers, whereas models of data for each breed and sex fit the effect of interaction of abattoir by butcher only. Due to possible future changes in back fat control, we performed a log transformation of BF. Our new models fit better than the currently used model.
Objectives : The objectives of this study is to develop a mouse model similar to Taeeum-type by inducing Lung fibrosis with bleomycin, and to determine adequate concentration of bleomycin. Methods : The subjects were divided into six groups: normal, obesity induced group, and bleomycin administered 0.015U, 0.03U, 0.06U, and 0.09U(U/100g bw) concentrations respectively. Each concentration of bleomycin was dissolved in distilled water, and administered through Intra-Nazal-Trachea injection method. Food intake and body weight were measured at regular time weekly. At the end of the experiment, blood was gathered by cardiac puncture for biochemical examinations, organs were removed for histological examinations, and weigh and mRNA genes was analyzed. Result : Mice administered with bleomycin at 0.015U and 0.03U showed body and fat weight gain, and increased blood total cholesterol, LDL-cholesterol, glucose, and free fatty acid level. Fat related genes also showed higher level than the control group. Obesity was most strongly induced in the mice administered with 0.03U of bleomycin. On the other hand, when bleomycin was administered at concentrations above 0.06U, a model of obesity mouse was not created due to rapid emphysema inflammation and weakness. Conclusions : Mice were most vulnerable to obesity when bleomycin was administered at a concentration of 0.3 to cause liver damage. Bleomycin concentration over 0.06U did not cause obesity-induced mice, due to severe damage in liver.
An experiment was conducted to evaluate the physiological changes of laying hens subjected to feed removal during induced molting while received probiotics in the drinking water. Post-molt performance and egg quality criteria were also studied. Ninety 78-week-old Hy-line W36 laying hens were divided into two treatment groups according to equal body weight and subjected to induced molting by continuous feed removal until around 30% BW reduction. The experiment lasted 12 wks consisting of 4-wk molting and 8-wk post-molt periods. Treatment 1 received no probiotics and was considered as the control. Treatment 2 was similar to the control except that hens received probiotics in the drinking water at 400 mg/L during feed deprivation. The results indicated that hens in both groups went out of production by Day 5. However, hens received probiotics reached 5 and 50% egg production sooner than the control (30 and 52 days vs. 31 and 54 days). Starvation during molting increased heterophil to lymphocyte (H/L) ratio, hematocrit and plasma T4 and $Na^+$ levels while plasma T3 and Cl- levels were decreased. Probiotics had no significant impact on BW reduction during molt. Post-molt egg production and egg mass were higher in hens which previously received probiotics, but these responses were not significant. However, feed conversion ratio was significantly better in hens which received probiotics. Hematocrit, plasma thyroid hormone concentrations (T3 and T4) and plasma $Na^+$, $K^+$ and Cl- levels during molting were not significantly influenced by supplementation of probiotics. However, H/L ratio showed a significant (p<0.05) reduction in birds which received probiotics suggesting beneficial effects of this product for feed-deprived laying hens. No significant difference was observed in post-molt egg quality criteria.
Nejad, Jalil Ghassemi;Lee, Bae-Hun;Kim, Byong-Wan;Ohh, Sang-Jip;Sung, Kyung Il
Asian-Australasian Journal of Animal Sciences
/
v.29
no.3
/
pp.378-383
/
2016
The objective of this study was to determine the effects of chromium methionine (Cr-Met) chelate supplementation on blood metabolites and fatty acid profile of beef from Holstein steers during late fattening period. Fifteen Holstein steers were allotted randomly into two groups including the control (non Cr-Met feeding, NCM, ave. body weight [BW] = $483{\pm}25.7kg$) and the treatment (Cr-Met feeding for 4 months, 4CM, ave. $BW=486{\pm}27.5kg$) group. The feeding amount of Cr-Met to animals was limited to 400 ppb/cow/d and was supplemented to total mixed ration. No difference in blood albumin, alkaline phosphatase, urea-nitrogen, calcium, creatine, glucose, total protein, triglyceride, and cholesterol were observed between the treatment groups (p>0.05). The level of high density lipoprotein was higher in the 4CM group than the NCM group, whereas low density lipoprotein was lower in the 4CM group (p<0.05). The fatty acid composition (caprate, laurate, myristate, pentadecanoate, palmitate, palmitoleate, margarate, cis-11 heptadodecanoate, stearate, oleate, trans-vaccenate, linoleate, cis-11 eicosenoate, docosa hexaenoic acid, and docosa pentaenoic acid) of the beef showed no difference between the two groups (p>0.05). The arachidonic acid level tended to be higher in the 4CM than the NCM group (p = 0.07). Cr-Met had no influence (p>0.05) on the ratio of saturated, unsaturated, unsaturated/saturated, monounsaturated/saturated and polyunsaturated/saturated fatty acids whereas the ratio of polyunsaturated fatty acids (PUFA) in the 4CM group was comparatively higher than the NCM group (p<0.05). This study concluded that feeding Cr-Met supplementation in 400 ppb/d to Holstein steers for 4 months during late fattening period can improve some blood metabolites and beef quality by increasing PUFA and gamma-linoleate compositions of beef.
Objective: Shearing is one of the practices that is applied periodically to fiber producing animals, which can also alter resistance of animals to high temperatures in especially summer months. This study aimed to investigate effects of shearing on some physiological and hormonal parameters in Akkaraman sheep during summer season. Methods: This study was carried out on 39 non-pregnant Akkaraman ewes (aged 1.5 years at the beginning of experiment). The 39 ewes were chosen randomly from the flock belonging to the Erciyes University and they were assigned to two groups as follows: i) group A (n = 20) designed as the control group, they were shorn and group B (n = 19) designed as the experimental group, they were unshorn. Prior to the shearing (-1 day) and on days 1, 7, 15, 30, 45, 60, 75, and 90 following the shearing, blood samples were taken from the vena jugularis of each sheep. Cortisol, β-endorphin, growth hormone (GH), thyroxine (T4), triiodothyronine (T3), and heat shock protein 70 (HSP-70) concentrations were determined using the enzyme immunoassay method. Body weight (BW), rectal temperature (RT), pulse rate (PR), and respiratory rate (RR) of each sheep were recorded at the same time. The data obtained were analyzed using two-way repeated measures analysis of variance. Results: Statistical analysis showed a significant effect of shearing×period interaction (p<0.01) and a significant effect of period (p<0.01) on BW, HSP-70, cortisol, T4 and RT, PR, GH, β-endorphin, T3, respectively. Also these analysis showed no significant effect of shearing×period interaction or period on RR. Conclusion: The results showed that the thermoregulation abilities of sheep were affected by shearing treatment and the shorn ewes were less affected by heat stress. In conclusion, based on the data of this study, shearing can be considered as a necessary management practice that requires protection for sheep from the effect of heat stress.
Background: Physical and chemical properties of feedstuffs can be changed by feed processing. Moreover, through various mechanisms, feed processing can affect growth performance and feed efficiency of swine, nutrition value of the feed. Weaning-to service-intervals (WSI), subsequent farrowing rates, and total-born litter sizes were determined by feed intake and metabolic state during lactation. Methods: A total of 20 sows (Landrace ${\times}$ Yorkshire) with an average body weight (BW) of 266.1 kg 4 d before farrowing were used to determine the effect of feed processing on the performance of lactating sows and their offspring. The following two dietary treatments were used: 1) Crumble diet (C); and 2) Mash diet (M). Ten replications were used for each treatment. Back fat thickness of sows was measured 6 cm off the midline at the 10th rib using a real-time ultrasound instrument at 4 d before farrowing, 1 d after farrowing, and during weaning. Sow BW were also checked at 4 d before farrowing, 1 d after farrowing, and during weaning. Fecal score of sows were assessed on d 14. Fecal score of piglets were observed on d 7, 15, and 24. Data were analyzed using t-test procedure of SAS (2014) with sow as experimental unit. Results: No significant (p > 0.05) difference was observed in the reproduction performance of sows between the two treatments. In addition, there was no significant (p > 0.05) difference in the growth performance of piglets between the two treatments. Fecal score of sows or piglets showed no significant (p > 0.05) difference either. Conclusions: In conclusion, different feed processing (mash or crumble) did not make any significant difference on the performance of lactation sow or their piglets.
The effects of genetic, physiological, and environmental factors(temperature, and type of watering system) on growth factors, accumulation of abdominal fat(AF), and the association between growth factors and AF of broilers and Athens-Canadian Randombred (ACRB) were studied in a series of nine trials. The final BW(6 or 7 wk) and 4-6 or 5-7 wk gain (G) were greater in groups raised at 21.1˚C than 26.7˚C. There was no consistent effects of environmental temperature on feed conversion ratio (FCR) although the FCR was significantly (P$\leq$.o5) reduced in 21. 1˚C group compared with the 26.7˚C group in two of six trials. The overall mean weight of AF relative to BW at 43 or 50 days of age were not significantly different between temperature groups: the means of AF of 21.1˚C and 26.7˚C groups were 1.81 and 1.78%, respectively. Environmental temperature had no consistent effect on the relationship between 4-6 or 5-7 wk C and 43 or 50 days AF, and 4-6 wk FCR and 43-days AF ; however, the association of 50 days AF with 5-7 wk FCR was highly significant in both temperature groups. The overall mean of correlation coefficient between FCR and AF was very low (r=.107). The waterer types did not significantly affect the AF. Generally, the effect of sex on the relationship between G and AF was not consistent in both sex groups. However, the association of AF with G was much greater in ACRB group compared with broiler group. The initial body weights (4 or S wk) was significantly (P$\leq$.001) correlated with AF in ACRB group, but not in the broiler groups. The effect of sire on AF was very high(P$\leq$.0001) in five of six trials.
The purpose of this study was to investigate the effects of glucuronic acid (isolated from xylan) on antioxidative defense system in rat after aerobic exercise. The glucuronic acid was isolated from xylan. Sprague-Dawley male rats weighing 150$\pm$10 g were randomly assigned to one normal group and three exercise training groups. Exercise training groups were classified to T (glucuronic acid free diet), TU (250 mg glucuronic acid/kg bw) and 2TU (500 mg glucuronic acid/kg bw) according to the level of glucuronic acid supplementation before exercise training. The experimental rats in exercise training groups (T, TU and 2TU) were exercised on glucuronic acid supplementation or rats in normal group (N) were confined in cage for 4 weeks. And rats were sacrificed with an overdose of pentobarbital injection just after running. Body weight, food intakes and food efficiency ratio (FER) were lower in the exercise training group than in the normal group. White gastrocnemius xanthine oxidase (XOD) activity in the T group was 85% greater than that of the normal group, whereas in the TU and 2TU groups it did not differ from the normal group. White gastrocnemius superoxide dismutase (SOD) activity in T group, that was decreased by 22% compared with that of N group, but those of TU and 2TU groups were increased by 38% and 42%, respectively, compared with that of T group. White gastrocnemius glutathione peroxidase (GSHpx) activity in T group, that was decreased by 42% compared with that of N group, but those of TU and 2TU groups were increased by 67% and 68%, respectively, compared with that of T group. Glutathione S-transferase (GST) activity of white gastrocnemius in N group was not significantly different from that in the T and TU groups, but 2TU group were increased by 12%. Contents of thiobarbituric acid reactive substance (TBARS) in T group was increased by 54%, compared with that of normal group but those of TU group and 2TU group were lower 44% and 36% than that of T group. In conclusion, the effects of glucuronic acids in exercise training rats would appear to reduce peroxidation of tissue as an antioxidative defense mechanism.
Chu, K.S.;Kim, J.H.;Chae, B.J.;Chung, Y.K.;Han, In K.
Asian-Australasian Journal of Animal Sciences
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v.11
no.3
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pp.249-254
/
1998
Two experiments were conducted to evaluate the effects of processing of barley on the growth performance and ileal and fecal digestibility of growing pigs. In Exp. 1, a total of 20 cannulated pigs (10.80 kg BW) were allotted to four treatments. Treatments were coarse ground barley as a control (CON), finely ground barley (FINE), extruded barley (EXT) and enzyme supplemented coarse ground barley (ENZ). In Exp. 2, a total of 100 growing pigs (36.50 kg BW) were allocated to the same treatments in completely randomized block design based on sex and body weight. In the first trial, pigs fed extruded barley showed significantly higher crude protein digestibility over pigs fed finely ground barley (p < 0.05). Pigs fed finely ground barley generally showed lower nutrients digestibility. Extrusion and ${\beta}$-glucanase supplementation showed a trend to improve nutrients digestibility. However, fine grinding rather reduced nutrients digestibility. The similar trend was found in the digestibility of essential amino acids. Fine grinding of barley significantly reduced amino acids digestibility. Extrusion and enzyme supplementation were found to improve amino acids digestibility of barley in growing pigs. In the growth trial, pigs fed extruded barley grew significantly faster than any other processed barley fed pigs. And extrusion of barley significantly improved feed/gain of pigs (p < 0.05). Fine grinding of barley and enzyme supplementation did not improve growth performance of pigs. In conclusion, fine grinding and enzyme supplementation does not appear to be an economical feed processing for growing pigs when barley is employed in the diets, while extrusion can be recommended as an effective feed processing technique for barley.
Noh, Hye-Ji;Koh, Hong Bum;Kim, Hee-Kyoung;Cho, Hyang Hyun;Lee, Jeongmin
Nutrition Research and Practice
/
v.11
no.1
/
pp.11-16
/
2017
BACKGROUND/OBJECTIVES: Helicobacter pylori (H. pylori) colonization of the stomach mucosa and duodenum is the major cause of acute and chronic gastroduodenal pathology in humans. Efforts to find effective anti-bacterial strategies against H. pylori for the non-antibiotic control of H. pylori infection are urgently required. In this study, we used whey to prepare glycomacropeptide (GMP), from which sialic acid (G-SA) was enzymatically isolated. We investigated the anti-bacterial effects of G-SA against H. pylori in vitro and in an H. pylori-infected murine model. MATERIALS/METHODS: The anti-bacterial activity of G-SA was measured in vitro using the macrodilution method, and interleukin-8 (IL-8) production was measured in H. pylori and AGS cell co-cultures by ELISA. For in vivo study, G-SA 5 g/kg body weight (bw)/day and H. pylori were administered to mice three times over one week. After one week, G-SA 5 g/kg bw/day alone was administered every day for one week. Tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), IL-$1{\beta}$, IL-6, and IL-10 levels were measured by ELISA to determine the anti-inflammatory effects of G-SA. In addition, real-time PCR was performed to measure the genetic expression of cytotoxin-associated gene A (cagA). RESULTS: G-SA inhibited the growth of H. pylori and suppressed IL-8 production in H. pylori and in AGS cell co-cultures in vitro. In the in vivo assay, administration of G-SA reduced levels of IL-$1{\beta}$ and IL-6 pro-inflammatory cytokines whereas IL-10 level increased. Also, G-SA suppressed the expression of cagA in the stomach of H. pylori-infected mice. CONCLUSION: G-SA possesses anti-H. pylori activity as well as an anti-H. pylori-induced gastric inflammatory effect in an experimental H. pylori-infected murine model. G-SA has potential as an alternative to antibiotics for the prevention of H. pylori infection and H. pylori-induced gastric disease prevention.
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