• 한국식품영양과학회지
• /
• 제30권3호
• /
• pp.521-527
• /
• 2001

The effects of dietary restriction (DR) on antioxidant enzymes were studied in liver, lung and erythrocytes of diabetic rats. Experimental animals used Sprague-Dawley (SD; body weight 350$\pm$20g) male rats and Otsuka Long Evans Tokushima fatty (OLETE; body weight 5--$\pm$30g) male rats, as a model of type 2 diabetes mellitus. Type I diabetes was induced in SD rats by intramuscular injection of alloxan (80 mg/kg BW). Animals were randomly assigned either to continue the ad libitum diet or 40% DR (60% intake of ad libitum diet) groups. The body weight was measured at every 2 weeks to 4 months following DR. The activities of antioxidant enzymes (superoxide dismutase (SOD), catalase, glutathione peroxidase (GSHPx) were measured in liver, lung and erythrocytes and the concentration of TBARS as a marker of reactive oxygen species-induced tissue injry was also measured in rats after 4 months 40% DR. The body weight 4 months after 40% DR of control SD, alloxian-diabetid SD and OLETE rats were 80%, 98% and 75% of each control groups, respectively. The activities of SOD, catalase and GSHPx in lung and erythrocytes of rats were not change by 40% DR but in 4 month 40% DR rat liver, the activities of SOD and catalase were increased in control SD, alloxan-diabetic SD, and OLETF groups. The concentration of TBARS in lung and erythrocytes was also not changed by 40% DR, while liver TBARS concentration was decreased in OLETF and control SD rats compared to each non-DR control rats. These results suggested that the body weight changes in diabetic rats by DR was more prominent in type 2 diabetes and changes of antioxidant enzymes is most prominent in liver by DR either type 1 and 2 diabetic rats.

• Asian-Australasian Journal of Animal Sciences
• /
• 제11권1호
• /
• pp.21-29
• /
• 1998

Four feeding trials with 260 pigs were conducted to evaluate the effects of supplementing the diet with different amino acids on growth performance and blood metabolites for weanling, growing and finishing pigs. One hundred twenty weanling pigs (Exp. 1, BW 8 kg), eighty growing pigs (Exp. 2. BW 20 kg), thirty growing pigs (Exp. 3, BW 29 kg) and thirty finishing pigs (Exp. 4, BW 50 kg) were randomly allotted to different dietary treatments according to sex and body weight. Pigs weight and feed consumption were measured at initiation and termination of each trial with 4 weeks. At the end of trial, blood samples from three pigs selected in each pen (Exp. 1) and each pig (Exp. 2) were obtained to determine the level of blood urea nitrogen, glucose, insulin and cortisol in the serum. In Exp. 1, pigs fed diet supplemented both with lysine and methionine had the best feed conversion ratio (p < 0.05), but no significant differences (p > 0.05) were observed in ADG and ADFI. Pigs receiving control diet obtained the obtained the optimal ADG (p < 0.05), ADFI (p < 0.05) and F/G for the whole period. No differences were detected in serum glucose, insulin and cortisol concentrations. In Exp. 2, pigs receiving the control diet exhibited the lowest serum urea nitrogen (p < 0.05), ADG, F/G and serum insulin concentration increased linearly (p < 0.05) with the inclusion of lysine, methionine, threonine and tryptophan in diets. No significant differences (p > 0.05) were detected for glucose and cortisol content in pigs serum among dietary treatments. In Exp. 3 and 4, pigs growth rate increased linearly (p < 0.01), and feed conversion efficiency was also improves by addition of lysine, methionine, threonine and tryptophan. In conclusion, pigs fed diets supplemented with lysine, methionine, threonine and tryptophan together obtained optimal growth performance in growing and finishing periods.

• Animal Bioscience
• /
• 제34권4호
• /
• pp.471-481
• /
• 2021

Objective: A comprehensive study was conducted to study the effects of partition of variance on accuracy of genetic parameters and genetic trends of economic traits in Vanaraja male line/project directorate-1 (PD-1) chicken. Methods: Variance component analysis utilizing restricted maximum likelihood animal model was carried out with five generations data to delineate the population status, direct additive, maternal genetic, permanent environmental effects, besides genetic trends and performance of economic traits in PD-1 chickens. Genetic trend was estimated by regression of the estimated average breeding values (BV) on generations. Results: The body weight (BW) and shank length (SL) varied significantly (p≤0.01) among the generations, hatches and sexes. The least squares mean of SL at six weeks, the primary trait was 77.44±0.05 mm. All the production traits, viz., BWs, age at sexual maturity, egg production (EP) and egg weight were significantly influenced by generation. Model four with additive, maternal permanent environmental and residual effects was the best model for juvenile growth traits, except for zero-day BW. The heritability estimates for BW and SL at six weeks (SL6) were 0.20±0.03 and 0.17±0.03, respectively. The BV of SL6 in the population increased linearly from 0.03 to 3.62 mm due to selection. Genetic trend was significant (p≤0.05) for SL6, BW6, and production traits. The average genetic gain of EP40 for each generation was significant (p≤0.05) with an average increase of 0.38 eggs per generation. The average inbreeding coefficient was 0.02 in PD-1 line. Conclusion: The population was in ideal condition with negligible inbreeding and the selection was quite effective with significant genetic gains in each generation for primary trait of selection. The animal model minimized the over-estimation of genetic parameters and improved the accuracy of the BV, thus enabling the breeder to select the suitable breeding strategy for genetic improvement.

• Journal of Animal Science and Technology
• /
• 제66권1호
• /
• pp.135-144
• /
• 2024

The objective of this study was to investigate the influence of feeding frequency on a sow's reproductive performance and stress response during gestation. A total of twenty multiparous sows (Yorkshire × Landrace) were used in a completely randomized design based on their parity, body weight (BW), and backfat thickness (BFT), and the sows were allotted to two different feeding systems: 1) once daily feeding (OF) and 2) twice daily feeding (TF) in corn-soybean meal based diets. The gestation diet was formulated to contain 3,265 kcal of metabolizable energy (ME) / kg, 12.90% of crude protein (CP), and 0.75 % of total lysine. The lactation diet was formulated to contain 3,265 kcal of ME / kg, 16.80% of CP, and 1.08% of total lysine and provided ad libitum during lactation. In gestation, sow BFT and BF changes were not affected by feeding frequency, but higher BW and BW gain from day 35 to 90 and day 35 to 110 were observed in OF sow (p < 0.10). In lactation, feeding frequency did not influence on BW, BW gain, BFT, BF changes, average daily feed intake, and wean-to-estrus interval. Also, there were no differences in litter size, litter weight and piglet weight in lactating sows. OF sows had higher (p < 0.05; p < 0.10) protein, solid-not-fat, and total solid concentrations in colostrum compared to TF sows, while OF sows had a lower (p < 0.05) lactose concentration in colostrum compared to TF sows. Sows in OF showed significantly lower average daily water consumption (ADWC) from day 35 to 110 of gestation (p < 0.05). While there were no significant differences in stereotypic behaviors and salivary cortisol levels during gestation between treatments, the OF sows showed less time spending on the activity at day 105 (p < 0.05). In conclusion, reduced feeding frequency increased BW gain during gestation, decreased activation time, and changed the colostrum composition. This information may contribute to the understanding of the physiological and behavioral change of gestating sows by manipulating feeding frequency.

• 한국수산과학회지
• /
• 제47권6호
• /
• pp.1063-1068
• /
• 2014

We investigated the effects of feeding rate on the growth performance and blood components of sub-adult olive flounder Paralichthys olivaceus. Optimum feeding rate (initial fish mean weight : $370{\pm}5.72g$) was determined under the low water temperature. Two replicated groups of fish were fed a commercial diet at rates of 0%, 0.3%, 0.4%, 0.5%, and 0.6% body weight (BW) per day, and to satiation (0.75%). Feeding trial was conducted under a flow-through system with 12 1.2-metric ton aquaria receiving filtered seawater at $12-14^{\circ}C$ for 4 weeks. Weight gain (WG) and specific growth rate (SGR) for fish fed at 0.6% BW per day was significantly higher than that of unfed fish (0%) and fish fed at 0.3% and 0.4% BW per day. There were no significant differences in WG and SGR among fish fed at 0.5%, 0.6%, and 0.75%. These parameters were negative and significantly lower in the starved fish than in fish fed the experimental diet at all feeding rates. Survival for unfed fish (0%) was significantly lower than that of fish fed at 0.4%, 0.5%, and 0.6%. Hematocrit and hemoglobin content of fish fed at 0% and 0.75% (satiation) were significantly lower than that of fish fed at 0.4% BW per day. Total protein content in unfed fish was significantly lower than those in other treatments. Broken-line regression analysis of weight gain showed that the optimum feeding rate of olive flounder weighing 370 g was 0.51% BW per day at the low water temperature.

• 대한수의학회지
• /
• 제61권4호
• /
• pp.38.1-38.8
• /
• 2021

This study examined the anti-diabetic effects of aqueous extracts of Dendropanax morbifera leaves (DMWEs) in streptozotocin-induced diabetic Sprague-Dawley (SD) rats. Thirty male SD rats (body weight [BW], 250.4 ± 19.7 g) were divided into the following six groups: normal control rats (NC), diabetic control rats (DC), diabetic rats treated with metformin HCl 100 mg/kg BW (DT), diabetic rats treated with DMWEs 50 mg/kg BW (DM-50), diabetic rats treated with DMWEs 100 mg/kg BW (DM-100), and diabetic rats treated with DMWEs 200 mg/kg BW (DM-200). From two weeks of administration of DMWEs, the BW of all groups treated with DMWEs increased significantly compared to DC (p < 0.05). At four weeks after treatment, the blood glucose levels in DT, DM-100, and DM-200 decreased below 200 mg/dL, while the glycated hemoglobin concentrations in all groups administered DMWEs were similar to those of NC and DT. Regarding the blood biochemical parameters, the levels of aspartate transaminase, alanine transaminase, blood urea nitrogen, and creatinine in DM-100 and DM-200 were similar to those in NC and DT. Overall, these results highlight the effectiveness of DM-100 in the treatment of diabetes.

• Asian-Australasian Journal of Animal Sciences
• /
• 제32권6호
• /
• pp.842-848
• /
• 2019

Objective: Heat stress poses an increasing threat for poultry production. Some amino acids have been found to play critical roles in affording thermotolerance. Recently, it was found that in ovo administration of L-leucine (L-Leu) altered amino acid metabolism and afforded thermotolerance in heat-exposed broiler chicks. Methods: In this study, two doses (35 and $70{\mu}mol/egg$) of L-Leu were administered in ovo on embryonic day 7 to determine their effect on rectal temperature (RT), body weight (BW) and thyroid hormones at hatching. Changes in RT, BW, and thermotolerance in post-hatched chicks were also analyzed. Results: It was found that in ovo administration of L-Leu dose-dependently reduced RT and plasma thyroxine ($T_4$) level just after hatching. In post-hatched neonatal broiler chicks, however, the higher dose of L-Leu administered in ovo significantly increased RT without affecting BW gain. In chicks that had been exposed to heat stress, the RT was significantly lowered by in ovo administration of L-Leu (high dose) in comparison with the control chicks under the same high ambient temperature (HT: $35^{\circ}C{\pm}1^{\circ}C$, 120 min). Conclusion: In ovo administration of L-Leu in a high dose contributed to an increased daily body temperature and afforded thermotolerance under HT in neonatal broiler chicks.

• Animal Bioscience
• /
• 제37권8호
• /
• pp.1463-1473
• /
• 2024

Objective: Three experiments were conducted to evaluate the effects of Saccharomyces yeast postbiotics (SYP) in feeds for sows on the growth of offspring (Exp. 1), for nursery pigs on their growth (Exp. 2), and for nursery and finishing pigs on their growth (Exp. 3). Methods: Exp. 1 had 80 sows at breeding assigned to 4 groups with SYP at 0, 0.050, 0.175, and 0.500 g/kg. Offspring were fed a common diet for 126 d. Exp. 2 had 144 barrows at 8 kg body weight (BW) allotted to CON (no SYP); YPC (SYP at 0.175 g/kg; d 0 to 42); and YPD (SYP at 1.25, 0.75, and 0 g/kg; d 0 to 7, d 8 to 21, and d 22 to 42, respectively) with 8 pens/treatment (6 pigs/pen). Exp. 3 had 96 barrows at 8 kg BW allotted to CON (no SYP); YPN (SYP at 0.175 g/kg; d 0 to 42); YPF (SYP at 0.100 g/kg; d 43 to 119); and YPA (SYP at 0.175 and 0.100 g/kg; d 0 to 42 and d 43 to 119, respectively) with 8 pens/treatment (3 pigs/pen). Results: In Exp. 1, increasing SYP increased (p<0.05, quadratic) the sow body score (maximum at 0.30% SYP), reduced (p<0.05, quadratic) the days-wean-to-estrus (minimum at 0.27% SYP), and increased (p<0.05) offspring BW at weaning and their average daily gain (ADG) and feed efficiency (G:F) at d 126. In Exp. 2, ADG, average daily feed intake (ADFI), and G:F of YPC were the greatest (p<0.05). The ADG and ADFI of YPD were greater (p<0.05) than CON. Fecal score of YPC and YPD was smaller (p<0.05) than CON. In Exp. 3, YPA had the greatest (p<0.05) ADG and YPN and YPF had greater (p<0.05) ADG than CON. Conclusion: SYP enhanced sow performance, offspring growth, growth of nursery and growing pigs with the greater efficacy at 0.27 to 0.32 g/kg feed.

• 환경생물
• /
• 제23권1호
• /
• pp.27-31
• /
• 2005

대하(Fenneropenaeus chinensis) 종묘생산에 관한 초기성장의 자료를 얻기 위하여, 충청남도 태안군 안면읍 창기리 앞바다에서 1993년 5월에 자망으로 모하를 채집하여 실험에 사용하였다. 모하의 평균 전장은 23.6±0.71cm, 평균 두흉갑장은 5.1±0.63cm, 평균 체중은 61.3±5.12 g이었다. 자연수온보다 4℃ 높은 22℃, 염분은 33‰ 조건에서 산란유발을 실시하였으며, 산란 후 FRP 1톤 용량의 원형수조에서 수온 24.0±0.5℃, 염분 33.0±1.0‰로 조절하여 수용 후 30시간 내외에서 90% 이상을 부화시켰다. 부화 후 Nauplius stage에는 먹이를 공급하지 않았으며, Zoea stage부터 먹이생물을 공급하였다. 이 시기부터 대량 배양한 Diatom(Chaetoceros simplex, Skeletonema costatum, Thalassiosira weissflogii)을 서로 같은 비율로 혼합하여 공급하였다. 먹이 밀도는 유생 100마리당 10만 cell mL/sup -1/로 유지하였으며, 이때 사육수온은 23.5±0.5℃였다. Nauplius의 형태는 앞부분은 둥글며 크고 뒷부분은 길고 가늘었다. 본 연구 결과, Nauplius의 크기는 부화 후 4일까지 평균 전장 0.21∼0.39mm였으며, 이후 다음 단계인 Zoea stage로 변태까지는 5회의 탈피 후 그 크기는 0.62∼l.03mm로 성장하였다. Zoea stage의 3번에 걸친 변태 후 Mysis stage는 부화 후 9일째에 평균 전장 1.28mm로 시작하여 3번의 변태를 거쳐 부화 후 17일째 평균 전장 5.98mm로 나타났다. Mysis stage의 3회 탈피 후에 Post larva에로의 변태는 부화 후 약 21일째 이었으며, 이후 점차로 수조 저면을 기어다니고 활발한 유영 능력을 보이는 치하로 변태하였다. 먹이생물을 혼합 공급한 본 연구는 여타 단독 먹이생물 공급 연구들과 비교시 높은 초기성장을 보였다. 또한 체중성장에 있어 변태에 의한 단계적인 성장을 나타냈다. 부화 후 25일까지의 전장에 대한 체중의 상대 성장식은 BW = 2E-5TL/sup 4.1707/ (r²= 0.9746) (TL: 전장; BW:체중)이었다.

• Journal of Animal Science and Technology
• /
• 제54권6호
• /
• pp.395-400
• /
• 2012

This study was conducted to estimate the growth curve parameters for the body weight (BW) and body length (BL) of miniature pigs in Korea. Growth curve parameters were estimated through a nonlinear regression model using Gompertz, Logistic, and von Bertalanffy methods. A total of 25 piglets were measured monthly from birth up to 15 months of age to estimate both body weight and length. Results showed that the estimated average values for the body weight (body length) were 31.83 kg (58.77 cm) for the mature weight (A), 3.06 (1.74) for the growth ratio (${\beta}$), and 0.28 (0.52) for the maturing rate (${\kappa}$). Average inflection points showing maximum growth rate estimated each month for body weight were 3.97 kg and 11.70 cm, while for the body length were 1.06 kg and 21.61 cm. Moreover, the estimated maturation rates of the body weight and length for the group of Sire 1 were 0.22 and 0.40 respectively, whereas for the group of Sire 2 these values were 0.34 and 0.39. On the other hand, for the groups of Dam 1, Dam 2, and Dam 3, maturation rates for their body weights were 0.26, 0.28 and 0.33 respectively, while for their body lengths these values were 0.43, 0.37, and 0.38, respectively. The study also indicated a negative relationship between the values of mature weight and maturity rate for the body weight will result to a higher inflection point which is in contrast for the body length where results show that a positive relationship between the values of mature length and the maturity rate will result to a higher inflection point. Furthermore, the growth performance of miniature pig varies across stages but using these estimated growth curve parameters could improve the genetic traits of miniature pig.