• Development and Reproduction
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• v.20 no.3
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• pp.187-196
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• 2016

The objective of the current study was to determine acute plasma stress responses in two size groups of juvenile Epinephelus akaara (average body weight: $8.4{\pm}2.1$ and $3.3{\pm}0.6g$; 150 and 120 days after hatch, respectively) exposed to abrupt salinity drops (from 34 practical salinity unit, PSU seawater to 18, 10 PSU (experiment 1) or 26, 18, 10 PSU (experiment 2), respectively). Plasma glucose, glutamic oxalate transaminase, glutamic pyruvate transaminase, red blood cell counts, and gill histology were determined during 72 h exposure. Significantly increased plasma glucose, glutamic oxalate transaminase levels, and red blood cell counts were observed in fish exposed to 18 or 10 PSU. Histological changes, such as hyperplasia and lifting of epithelium in the gill secondary lamellae, were also observed in fish exposed to 18 or 10 PSU at 72 h post-drop. E. akaara exposed to sudden salinity drops to 18 or 10 PSU still seems to undergo the primary adjustment phase before fish reaches a new homeostasis, whereas fish exposed to 26 PSU seems to mount osmotic changes. Therefore, the no observed adverse effect levels for 72 h acute salinity challenge was 26 PSU in our study, and salinity drop to 18 PSU and below can possibly cause acute adverse effect, in which fish could be vulnerable to additional stresses such as a temperature changes or handling stress.

• Journal of Dental Anesthesia and Pain Medicine
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• v.16 no.4
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• pp.289-294
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• 2016

Background: Control of postoperative pain is an important aspect of postoperative patient management. Among the methods of postoperative pain control, patient-controlled analgesia (PCA) has been the most commonly used. This study tested the convenience and safety of a PCA method in which the dose adjusted according to time. Methods: This study included 100 patients who had previously undergone orthognathic surgery, discectomy, or total hip arthroplasty, and wished to control their postoperative pain through PCA. In the test group (n = 50), the rate of infusion was changed over time, while in the control group (n = 50), drugs were administered at a fixed rate. Patients' pain scores on the visual analogue scale, number of rescue analgesic infusions, side effects, and patients' satisfaction with analgesia were compared between the two groups. Results: The patients and controls were matched for age, gender, height, weight, and body mass index. No significant difference in the mount of drug administered was found between the test and control groups at 0-24 h after the operation; however, a significant difference was observed at 24-48 h after the operation between the two groups. No difference was found in the postoperative pain score, number of side effects, and patient satisfaction between the two groups. Conclusions: Patient-controlled anesthesia administered at changing rates of infusion has similar numbers of side effects as infusion performed at a fixed rate; however, the former allows for efficient and safe management of postoperative pain even in small doses.

• Journal of Korean Neurosurgical Society
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• v.58 no.1
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• pp.9-13
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• 2015

Objective : To improve pedicle screw placement accuracy with minimal radiation and low cost, we developed specially designed K-wire with a marker. To evaluate the accuracy of thoracolumbar pedicle screws placed using the novel guide-pin and portable X-rays. Methods : Observational cohort study with computerized tomography (CT) analysis of in vivo and in vitro pedicle screw placement. Postoperative CT scans of 183 titanium pedicle screws (85 lumbar and 98 thoracic from T1 to L5) placed into 2 cadavers and 18 patients were assessed. A specially designed guide-pin with a marker was inserted into the pedicle to identify the correct starting point (2 mm lateral to the center of the pedicle) and aiming point (center of the pedicle isthmus) in posteroanterior and lateral X-rays. After radiographically confirming the exact starting and aiming points desired, a gearshift was inserted into the pedicle from the starting point into the vertebral body through the center of pedicle isthmus. Results : Ninety-nine percent (181/183) of screws were contained within the pedicle (total 183 pedicle screws : 98 thoracic pedicle screws and 85 lumbar screws). Only two of 183 (1.0%) thoracic pedicle screws demonstrated breach (1 lateral in a patient and 1 medial in a cadaver specimen). None of the pedicle breaches were associated with neurologic or other clinical sequelae. Conclusion : A simple, specially designed guide-pin with portable X-rays can provide correct starting and aiming points and allows for accurate pedicle screw placement without preoperative CT scan and intraoperative fluoroscopic assistance.

• Fashion & Textile Research Journal
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• v.18 no.3
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• pp.289-300
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• 2016

This study surveys children's costumes and games from the 11 Genre paintings by Junkeun Kim in the book "Korean Games(Stewart Culin 1858-1929)". The character of the general dress-costumes, games and the culture of life from the late $19^{th}$ century in genre painting of Junkeun Kim are as follows. The study results on the games are as follow. The boy's games are kite-flying, spinning tops, playing shuttlecock with the feet, blindman's buff, yut("Four-Stick Game"). Girl's games are seesaw with board, blindman's buff, marbles. Combined games are mount shoulder, sledge, tightrope walking for men with boy. The strengthen one's body type were seesaw with board, tightrope walking, the compete score type were playing shuttlecock with the feet, marbles and the multi complex type were yut("Four-Stick Game"), kite-flying. Through genre painting in the $19^{th}$ century we know a boy's 'Jeogori 'and 'Ba-ji' were similar to a modern man's and boy's 'Hanbok' with a traditional method of wearing. We believe that the originality of a traditional costume was an unchangeable characteristic. Girl's 'Jeogori' and 'Chi-ma' changed in length and width, and method of wearing; however, the basic shape did not change. The analysis for artist's genre painting which was ordered by a foreigner and the late of $19^{th}$ century's children's costume and game of life culture is useful to match the counters and show how to wear a modern Korean costume used to understand the 'Hanbok' and establish a costume of life.

• Journal of Embryo Transfer
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• v.26 no.2
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• pp.123-128
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• 2011

In vitro maturation and activation of oocytes are primary steps towards biotechnological manipulation in embryology. The objectives of the present study were to determine the oocyte recovery rate per ovary, in vitro maturation rates of oocytes and rates of parthenogenetically activation of matured oocytes in Black Bengal goats. All visible follicles were aspirated to recover follicular fluid from individual ovaries (number of ovaries = 456). The immature cumulus oocyte complexes (COCs; n = 1289) were cultured in tissue culture medium (TCM)-199 supplemented with 10% (v/v) fetal bovine serum (FBS) for 27 hours at $39^{\circ}C$ with 5% $CO_2$ in humidified air. The matured oocytes (n = 248) were activated with 5 ${\mu}M$ ionomycin for 5 minutes followed by treatment with 2 mM 6-dimethylaminopurine (6-DMAP) for 4 hours. After activation, oocytes were cultured for another 14 hours in TCM-199 supplemented with bovine serum albumin (BSA) at $39^{\circ}C$ with 5% $CO_2$ in humidified air. The pronucleus formation in activated oocytes was determined by staining with 1% orcein (whole mount technique). Matured oocytes (n = 176) without activation stimuli were used as control. The mean number of oocytes recovered per ovary was $3.5{\pm}0.5$. The proportion of oocytes matured in vitro, confirmed by the presence of first polar body, was $42.1{\pm}4.7%$. Parthenogenetic activation, evidenced by formation of pronucleus, occurred in $37.2{\pm}15.8%$ of matured oocytes. No pronucleus formation was observed in control oocytes. In conclusion, a combination of ionomycin and 6-DMAP induces activation in one third of Black Bengal goats' oocytes.

• Journal of fish pathology
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• v.19 no.2
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• pp.99-108
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• 2006

Diseased tiger puffer (Takijugu rubripes) showed anorexia and severe anemia in the gills. In wet mount preparations Heterobothrium sp. with 4 sets of clamps on the opisthohaptor attached to the gill filaments of the diseased fish. From the drum-shaped filter for filtration of the culturing water and the sediment of aquarium held the diseased fish in the laboratory, their eggs forming long strings through connection of the bipolar filaments could be easily collected. The parasites with 4 pairs of clamps on the opisthohaptor were 2.38mm in length and 0.71 mm in width, but had no isthmus which was separated the opisthohapor from the body proper. The parasites could be easily distinguished from Heterobothrium tetrodonis and H. okamotoi in the absence of distinct isthmus, and resembled H. yamagutii described from the gills of Takifugu xanthopterus in Japan. Their eggs were yellowish spindle in shape and 180-200 x 5-6 Iffil in size, which were pointed at both ends. The eggs were linked to adjacent egg shells like a string of beads by the filaments. Onchomiracidia, ciliated larvae spawned from the eggs were 133 Iffil (120-146 Iffil) x 751ffil (68-80 Iffil) in size. The length of the ciliates was 12 Iffil in the anterior part of the larvae and 14 Iffil in the posterior part. Round or leaf-shaped young parasites attached on the secondary gill lamellae. There was a tendency that most of round smaller parasites without an opisthohaptor were found in the middle part of the gill lamellae while leaf-shaped larger ones with a opisthohaptor attached on the distal part of the gill filaments. Each clamp of mature parasites grasped one secondary gill filament which resulted in bending of the filaments and hyperplasia of the epithelium and mucous cells. In the branchial cavity around the pseudobranch, mature parasites grasped the adjacent ones by means of the clamps, and the epithelia of the branchial cavity around the parasites showed severe irregular hyperplasia and erosion with strongly PAS-positive mucous cells.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2017.05a
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• pp.159-159
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• 2017

Ti-6Al-4V alloy have been used for dental implant because of its excellent biocompatibility, corrosion resistance, and mechanical properties. However, the integration of such implant in bone was not in good condition to achieve improved osseointergraiton. For solving this problem, calcium phosphate (CaP) has been applied as coating materials on Ti alloy implants for hard tissue applications because its chemical similarity to the inorganic component of human bone, capability of conducting bone formation and strong affinity to the surrounding bone tissue. Various metallic elements are known to play an important role in the bone formation and also affect bone mineral characteristics. Especially, Zn is essential for the growth of the human and Zn coating has a major impact on the improvement of corrosion resistance. Plasma electrolytic oxidation (PEO) is a promising technology to produce porous and firmly adherent inorganic Zn containing TiO2(Zn-TiO2)coatings on Ti surface, and the a mount of Zn introduced in to the coatings can be optimized by altering the electrolyte composition. In this study, effect of Zn content on the corrosion behavior of Ti-6Al-4V alloy after plasma electrolytic oxidation were studied by SEM, EDS, XRD, AC impedance, and potentiodynamic polarization test. The potentiodynamic polarization and AC impedance tests for corrosion behaviors were carried out in 0.9% NaCl solution at similar body temperature using a potentiostat with a scan rate of 1.67 mV/s and potential range from -1500 mV to +2000 mV. Also, AC impedance was performed at frequencies ranging from 10 MHz to 100 kHz for corrosion resistance.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2006.05a
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• pp.376-379
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• 2006

Reaction Wheel Assembly (RWA) is one of the major disturbance sources that have influence upon the Line of Sight (LOS) of payload. A micro-vibration induced by RWA is propagated through the satellite structure and decrease the LOS stability performance of payload. This effect shall be analyzed through the jitter analysis. If a requirement or specification of payload jitter level is found to be not satisfied according to the jitter analysis campaign, some modification or redesign should be done on the satellite structure or a couple of isolator should be attached on the RWA interface in order to reduce the transmitted vibration level of RWA. The purpose of ???RWA isolator test? is to roughly evaluate the performance of vibration suppression level with a passive RWA isolator made of rubber. For this test, actual RWA is used as a vibration source and a couple of cube-shaped rubber mount designed for satellite is used as a passive isolator. There may be several considerations in order to accommodate RWA isolator to spacecraft such as not only vibration reduction performance but also thermal conduction problem, mechanical size, RWA alignment problem, etc. But in this report the feasibility of RWA isolator is analyzed only in a vibration suppression point of view. As a result, high frequency vibration of RWA above 50Hz is perfectly attenuated with isolators, however, first harmonic components below 50Hz became larger due to the additional low frequency resonance modes of roll, pitch, yaw rigid body motion of RWA+bracket.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.8
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• pp.5051-5059
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• 2015

Structural behaviour of the front loader for an agricultural tractor was analyzed for three impact test conditions: drop and catch, corner pull, and corner push. Rigid-body dynamic, transient structural, and static structural analyses were conducted using a commercial finite element software. Analysis of the drop and catch test dealt with the case that the bucket located at the maximum elevation was dropped and catched through three steps. Analysis of the corner pull test dealt with the case that the bucket constrained to the ground by a chain at its corner was raised suddenly. Analysis of the corner push test dealt with the case that the corner of the bucket collided with an obstacle. Results of analyses of the three test conditions showed that maximum stress occurs at the geometrically discontinuous location in the mount and is caused from local stress concentration. Results of the present research can be utilized as a guideline to achieve more reliable and safe structural design of the front loaders.

• Journal of the Institute of Convergence Signal Processing
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• v.20 no.4
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• pp.226-231
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• 2019

A WTCI is an important criteria for evaluating an mount of patient's tongue coating in tongue diagnosis. However, Previous WTCI tongue coating evaluation methods is a most of quantitatively measuring ration of the extracted tongue coating region and tongue body region, which has a non-objective measurement problem occurring by exposure conditions of tongue image or the recognition performance of tongue coating. Therefore, a WTCI based on deep learning is proposed for classifying an amount of tonger coating in this paper. This is applying the AI deep learning method using big data. to WTCI for evaluating an amount of tonger coating. In order to verify the effectiveness performance of the deep learning in tongue coating evaluating method, we classify the 3 types class(no coating, some coating, intense coating) of an amount of tongue coating by using CNN model. As a results by testing a building the tongue coating sample images for learning and verification of CNN model, proposed method is showed 96.7% with respect to the accuracy of classifying an amount of tongue coating.