• Korean Journal of Ecology and Environment
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• v.45 no.2
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• pp.190-199
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• 2012

The reproductive ecology of an endangered species, Gobiobotia macrocephala (Pisces: Cyprinidae), was investigated in the Seom River, a tributary of the Han River drainage system, from March 2010 to February 2011. During the non-spawning season, no difference in the external morphology was observed between males and females; however, during the spawning season, clear differences merged for the depth and width of the body, preventral length and preanal length in the standard length. The sex ratio of females to males was 1 : 1.01. The spawning season was estimated to last from late April to middle of June, at water temperatures of $15{\sim}25^{\circ}C$. The number of mature eggs in the ovary averaged about $2,134{\pm}930$ (mean${\pm}$SD), and the mean diameter was approximately $0.88{\pm}0.04$ mm. The spawning area was composed of pebble and cobble bottoms (3~10 cm), with current velocities of 13~24 cm $s^{-1}$ and water depths of 12~18 cm. Spawning behavior between males and females was observed in a water tank 14~15 h after injecting females with Ovaprim to promote reproduction. Fish spawned near the water surface with monogamous pairing.

• Journal of fish pathology
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• v.9 no.2
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• pp.147-155
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• 1996

This study was carried out to examine the acute toxic effects of ammonia to the eel, Anguilla japonica in high temperature and pH levels by histopathological observations. The eels of 40 g average body weight were exposed to 4 different concentrations of total ammonia (0, 10, 20, 30 mg/$\ell$) for 24~120 hours. Each concentration was treated under 4 different levels of pH (7.5, 8.0, 8.5, 9.0) and each of these treatments was tested at 2 different temperatures ($27^{\circ}C$, $32^{\circ}C$). Histopathological changes in gill tissues were observed by hematoxylin-eosin stain. As increasing of pH (from 7.5 to 9.0), water temperature (from $27^{\circ}C$ to $32^{\circ}C$), total ammonia concentration (from 0 mg/$\ell$ to 30 mg/$\ell$) and exposure time (from 24 hours to 120 hours), gill discolorated to dark brown with the naked eye and gill tissues showed hypertrophy of gill lamellae, winding of the secondary gill lamellae, epitherial separation and necrosis histopathologically. When gill lamellae epithelium was separated from the blood vessels, gill discolorated to dark brown with the naked eye.

• Korean journal of applied entomology
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• v.35 no.4
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• pp.297-301
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• 1996

This test has been carried out to evaluate the effect of temperature on the growth of the insecticide susceptible strain, URY-O nomal genotype and insecticide resistant strain, O-RT abnormal genotype, and ABURABI nomal genotype. The nymphal periods were not significantly different between URY-O and O-RY strains at $25^{\circ}C$. At $30^{\circ}C$, susceptible strain URY-O could give birth to offsprings almost nomally, while resistant strain O-RY could not produce any offspring for 20 days which results in nymphal death. The numbers of offsprings of strain URY-O and strain ABURABI were not different between $25^{\circ}C\;and\;28^{\circ}C$, but strain O-RY, when it was reared at $28^{\circ}C$, could produce offsprings only 10% of those at $25^{\circ}C$. Body weight of strain URY-O and strain ABURABI were 0.22mg/female and 0.27mg/female, respectively at $28^{\circ}C$, however that of O-RY was only 0.16mg/female, showing considerable difference between normal and abnormal genotype. Substrain O-RY(+) which has high esterase activity showed poor reproduction ability(0.8 progenies per $G_{1}$ individual than substrain O-RY(-) (3.4 progenies per $G_{1}$ individual) which has low esterase activity at $28^{\circ}C$

• Korean Journal of Poultry Science
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• v.43 no.4
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• pp.213-218
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• 2016

This study was conducted to investigate the effect of taurine supplementation on heat shock protein 70 and in vitro protein turnover in broiler chicks under chronic heat stress. Chicks were allocated into 3 groups of 10 birds per group; the control group was maintained at a temperature of $24^{\circ}C$ without taurine (CO group), the heat-stressed group maintained at a temperature of $34^{\circ}C$ without taurine (HO group), and heat-stressed group maintained at a temperature of $34^{\circ}C$ with taurine (HT group). The final body and liver weights of broilers in the HO and HT groups were significantly lower than those of broilers in the CO group (P<0.05). However, these parameters of the broilers in the HT group were significantly higher than those of broilers in the HO group (P<0.05). The heat shock protein 70 (hsp70) concentration in the liver of broilers in the HO group was significantly higher than that of broilers in the CO and HT groups, but the hsp70 concentration in the liver of broilers in the HT group was not different from that of broilers in the CO group. Liver homogenates of 21 day-old broilers were incubated at temperatures of $37^{\circ}C$ and $45^{\circ}C$ to prove the effect of high temperature and taurine on total protein syntheses. Neither high temperature nor taurine supplementation affected protein syntheses in liver homogenates of the broilers. However, the more the temperature increased, the more the degradation rates of cytoplasmic protein in liver homogenates increased; however, taurine supplementation had no effects on the protein syntheses in the liver of the broiler. It is possible that taurine indirectly affected protein turnover via various physiological mechanisms.

• Journal of Conservation Science
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• v.30 no.3
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• pp.249-261
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• 2014

This study aims to examine production technique of white wares from the Guyre-2ri 1st and 2nd kiln sites, Wonju, Gangwon Province and characteristics of the used materials, and to find a correlation among materials of the excavated white wares. X-ray fluorescence sequential spectroscopy(XRF), X-ray diffraction(XRD), Dilatometer and Inductively coupled plasma mass spectrometry(ICP-MS), Inductively coupled plasma automic emission spectrometer(ICP-AES) were applied to determine the chemical composition, crystalline phase of samples, firing temperatures, trace elements and rare earth elements. White wares from the Guyre-2ri kiln sites contained high contents of coloring oxides and fluxes. Though firing temperature of each sample was different, they were mostly fired at a temperature below $1200^{\circ}C$ and some of them experienced a low temperature of $1000^{\circ}C{\pm}20^{\circ}C$ and a high temperature above $1200^{\circ}C$. When analyzing body crystalline phases of the white wares using the XRD method, quartz and mullite were extracted from all the samples, and the proportions were similar to each other. When analyzing the excavated white wares using the Seger formula, also, all the samples showed similar clay sources and production techniques. Moreover, the white wares were made of host rocks of the same geological origin, according to the result of rare earth elements analysis.

• Tuberculosis and Respiratory Diseases
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• v.71 no.1
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• pp.8-14
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• 2011

Background: MicroRNAs (miRNAs) have demonstrated their potential as biomarkers for lung cancer diagnosis. In recent years, miRNAs have been found in body fluids such as serum, plasma, urine and saliva. Circulating miRNAs are highly stable and resistant to RNase activity along with, extreme pH and temperatures in serum and plasma. In this study, we investigated serum miRNA profiles that can be used as a diagnostic biomarker of non-small cell lung cancer (NSCLC). Methods: We compared the expression profile of miRNAs in the plasma of patients diagnosed with lung cancer using an miRNA microarray. The data from this assay were validated by quantitative real-time PCR (qRT-PCR). Results: Six miRNAs were overexpressed and three miRNAs were underexpressed in both tissue and serum from squamous cell carcinoma (SCC) patients. Sixteen miRNAs were overexpressed and twenty two miRNAs were underexpressed in both tissue and serum from adenocarcinoma (AC) patients. Of the four miRNAs chosen for qRT-PCR analysis, the expression of miR-23a was consistent with microarray results from AC patients. Receiver operating characteristic (ROC) curve analyses were done and revealed that the level of serum miR-23a was a potential marker for discriminating AC patients from chronic obstructive pulmonary disease (COPD) patients. Conclusion: Although a small number of patients were examined, the results from our study suggest that serum miR-23a can be used in the diagnosis of AC.

• Journal of Aquaculture
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• v.17 no.4
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• pp.240-245
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• 2004

Two experiments were conducted to study the effect of temperature on growth and survival of yearling sea bass, Lateolabrax japonicus reared from 3$^{\circ}C$ to 33$^{\circ}C$. In the I st experiment, we used yearlings fish averaging 19.2$\pm$0.2 cm/fish (Mean$\pm$S.E.M.) of total length (TL) and 67.0$\pm$1.8 g/fish (Mean$\pm$S.E.M.) of body weight (BW), and we cultured the fish at 3, 6, 9, 12 and 17$^{\circ}C$ for 90 days. In the 2nd experiment, the experimental fish averaging 24.9$\pm$0.1 cm/fish (Mean$\pm$S.E.M) of TL and I 46.4$\pm$3.0 g/fish (Mean$\pm$S.E.M) of BW were reared at 21,24,27,30 and 33$^{\circ}C$ for 90 days. During these experiments, we measured food intake, feed efficiency, survival and growth (TL and BW) in the both experiments. Although food intake of the yearling increased with the temperature, the feed efficiency was only enhanced within the temperature range, from 21 to 27$^{\circ}C$. Growth of yearling was normal within the temperature range from 17 to 3$0^{\circ}C$, but it was stoped or reduced in other temperatures. Survival rate was significantly reduced in 3$^{\circ}C$ from the 1st experiment and in 30 and 33$^{\circ}C$ from the 2nd experiment, but there was no significant difference among other groups.

• Journal of the Korean Home Economics Association
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• v.31 no.1
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• pp.211-223
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• 1993

바닥마감재료가 다른 여름철의 찬바닥이 인체피부온도 및 온열적 쾌적감에 미치는 영향을 검토하기 위하여, 주위벽 및 천장의 온도를 기온과 거의 같게 한 항온실을 이용해서 바닥에 앉은 자세, 의자에 앉은 자세, 서 있는 자세별로 검토했다. 그 결과를 요약하면 다음과 같다. (1) 평균피부온도는 기온이 낮고 수온이 낮을수록 낮았다. Carpet 마감재료는 서 있는 자세, 바닥에 앉은 자세의 평균피부온도가 낮고 의자에 앉은 자세가 높았다. Cork 마감재료가 Carpet, Flooring 마감재료에 비해 평균피부온도가 낮았다. (2) 온냉감평가는 흑구온도가 낮게 되면$\ulcorner$중성이다$\lrcorner$혹은$\ulcorner$서늘하다$\lrcorner$쪽으로 평가하고, Cork, Carpet, Flooring 마감재료의 순으로 $\ulcorner$서늘하다$\lrcorner$쪽으로 평가 하였다. (3) 쾌적감평가는 바닥에 앉은 자세와 서 있는 자세가 의자에 앉은 자세보다 쾌적한 것으로 평가하였다. 마감재료는 Cork, Flooring, Carpet 순으로 쾌적하다고 평가 하였다. (4) 바닥마감재료에 대한 평가는 바닥에 앉은 자세의 경우, Carpet 마감재료는$\ulcorner$차가운$\lrcorner$$\ulcorner$습기찬$\lrcorner$의 평가점수가 높고, Cork 마감재료는$\ulcorner$평활한$\lrcorner$$\ulcorner$차가운$\lrcorner$$\ulcorner$감촉이 좋은$\lrcorner$$\ulcorner$딱딱한$\lrcorner$의 평가점수가 높고, Flooring 마감재료는$\ulcorner$평활한$\lrcorner$$\ulcorner$탄력성이 없는$\lrcorner$$\ulcorner$딱딱한$\lrcorner$의 평가점수가 높았다.$\ulcorner$따듯한$\lrcorner$-$\ulcorner$차가운$\lrcorner$의 평가는 바닥온도의 영향을 받아서, Cork, Flooring 마감재료가 Carpet 마감재료보다$\ulcorner$차가운$\lrcorner$의 높은 점수평가를 하였다. (5) Carpet 마감재료는 평균피부온도가 34$^{\circ}C$를 넘어도$\ulcorner$약간 시원하다$\lrcorner$는 평가이고, 서 있는 자세와 의자에 앉은 자세가 바닥에 앉는 자세보다 쾌적하다고 평가한다. Cork 마감재료는 3자세 모두 평균피부온도 33~34$^{\circ}C$의 사이에서 가장 쾌적하다. Flooring 마감재료는 온열적중성, 쾌적감의 중성의 평가가 많다. 이상의결과를 종합하면, 찬바닥이 인체에 미치는 영향은 바닥마감재료로는 Cork, Flooring이 보다 좋은 평가를 하였음으로, 여름철 바닥마감재료로서는 Cork, Flooring 마감재료가 바람직하다고 생각된다. 자세별로는 서 있는 자세는 바닥에 앉은 자세와 거의 같은 경향을 보이고, 의자에 앉은 자세는 바닥에 앉은 자세와 서 있는 자세 만큼 영향을 받지 않았다. 이후 실험결과를 중심으로 찬바닥의 경우, 보다 쾌적한 온열환경범위를 제안하려고 한다.

• Journal of Animal Environmental Science
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• v.15 no.2
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• pp.131-138
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• 2009

This study was conducted to find ways to control environment with the difference between body temperature and background temperature based on swine activity, and to apply to the environment control system of swine barns based on the findings. Following are the results. 1. Swine activity related to background temperature was achieved as color images and swine activity status was categorized into cold, comfortable, and hot periods with visualization system (thermal image system). 2. Thermal image system consisted of an infrared CCD camera, an image processing board - DIF (TH3100), an main computer (400Hz, 128M, 586 Pentium model) with C++ program installed. 3. Thermal image system categorizing temperatures into cold, comfortable, and hot was applicable to the environment control system of swine barns 4. Feed intake was higher in cold temperature, and finishing weight and weight gain per day in cold temperature were lower than others (p<0.05).

• Current Research on Agriculture and Life Sciences
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• v.26
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• pp.71-79
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• 2008

It is known that the content of asparagine, an excellent detoxifying substance of alcohols in human body, is the highest in the roots of soy-sprout. At the same time, it is inferred that soy-sprouts producing more roots are better for detoxifying. In this experiment, the effects of room temperatures on number of watering per day, and duration of soy-sprout culture were carefully investigated. Some of the results obtained are as follows; 1. The yield rate of soy-sprouts for Agakong and Pungsannamulkong was continuously increased up to 9 days. It was higher in room temperature of $30^{\circ}C$ than in $20^{\circ}C$ and was the highest at 8th day of culture with 5 times of watering per day. 2. The asparagine content in soy-sprouts of Agakong and Pungsannamulkong was the highest in cotyledon and the lowest in roots. This rate was higher in the room temperature of $20^{\circ}C$ than in $30^{\circ}C$. 3. The highest asparagine content of soy-sprout of Agakong was 18.9%, obtained in the room temperature of $30^{\circ}C$, cultivated for 8 days with 5 times of watering per day. 4. The highest asparagine content of Pungsannamulkong was 18.8%, obtained in hypocotyl cultivated in the room temperature of $30^{\circ}C$ for 8 days with the number of 2 times watering per day. 5. When an cultivation apparatus of 5 liters volume was used, the optimum seed amount for the highest yield rate was 300g for Agakong and 500g for Pungsannamulkong. At the same time, the number of lateral roots showed increasing tendency with more amounts of soybean seeds used.