• Asian-Australasian Journal of Animal Sciences
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• 제14권6호
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• pp.765-770
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• 2001

Thirty second parity sows of the synthetic Nepalese Pakhribas genotype were used to investigate factors which might improve the occurrence and expression of estrus. The experiment had two sequential elements. In part 1, a change in suckling pattern was applied during lactation, and in part 2, different estrus detection methods were evaluated after weaning. All sows received the same pattern of weaning, which imitated the progressive weaning system used in Nepalese villages. Piglets from each litter were weaned at three ages (6, 7 and 8 weeks of age) in the proportion of 0.5 at 6 weeks followed by 0.25 at each of the subsequent weanings. In the first lactation treatment, the suckling pattern was left undisturbed, similar to the practice used in the villages in which the remaining piglets after first weaning are allowed continuous suckling. In the other treatment, the remaining piglets after first weaning were allowed to suckle their sows only during the night, whilst in the day time (09:00-16:00) they were excluded from the sow but left free to roam around. After weaning, estrus detection procedures were carried out in the absence or presence of two different boar stimuli: a synthetic boar pheromone spray or fresh boar urine. These were applied sequentially in a sequence of testing that alternated for each sow on a daily basis. The weaning to re-mating interval was significantly longer for the unrestricted suckling treatment. All sows were re-mated within 30 days after first weaning in the restricted suckling treatment groups, whereas only 71% of sows were re-mated within 30 days after weaning in the unrestricted suckling treatment groups ($x^2=3.877$, 1df, p<0.05). Both boar pheromone spray and boar urine increased the estrus detection probability, with no significant differences between the two stimuli treatments.

• Journal of Veterinary Science
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• 제23권2호
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• pp.40.1-40.13
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• 2022

Background: Somatic cell nuclear transfer (SCNT) is used widely in cloning, stem cell research, and regenerative medicine. The type of donor cells is a key factor affecting the SCNT efficiency. Objectives: This study examined whether urine-derived somatic cells could be used as donors for SCNT in pigs. Methods: The viability of cells isolated from urine was assessed using trypan blue and propidium iodide staining. The H3K9me3/H3K27me3 level of the cells was analyzed by immunofluorescence. The in vitro developmental ability of SCNT embryos was evaluated by the blastocyst rate and the expression levels of the core pluripotency factor. Blastocyst cell apoptosis was examined using a terminal deoxynucleotidyl transferase dUTP nick end-labeling assay. The in vivo developmental ability of SCNT embryos was evaluated after embryo transfer. Results: Most sow urine-derived cells were viable and could be cultured and propagated easily. On the other hand, most of the somatic cells isolated from the boar urine exhibited poor cellular activity. The in vitro development efficiency between the embryos produced by SCNT using porcine embryonic fibroblasts (PEFs) and urine-derived cells were similar. Moreover, The H3K9me3 in SCNT embryos produced from sow urine-derived cells and PEFs at the four-cell stage showed similar intensity. The levels of Oct4, Nanog, and Sox2 expression in blastocysts were similar in the two groups. Furthermore, there is a similar apoptotic level of cloned embryos produced by the two types of cells. Finally, the full-term development ability of the cloned embryos was evaluated, and the cloned fetuses from the urine-derived cells showed absorption. Conclusions: Sow urine-derived cells could be used to produce SCNT embryos.

• 한국임상수의학회지
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• 제27권3호
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• pp.295-298
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• 2010

5살의 중성화된 수컷 풍산 개가 서혜부에 지속적인 출혈 증상으로 내원하였다. 내원 5일 전에 멧돼지에게 공격을 받은 병력을 가지고 있었으며, 양성 요도 조영술에서 막성 요도의 파열과 요도 피부 누공이 있음을 확인 하였다. 수술 중 요도 카테터를 장착하여서 요도와 요도 피부 누공을 확인 하였고, 오줌의 누출로 인해 발생한 괴사 조직과 손상된 조직을 제거 하였다. 요도 카테터를 장착하여 요도 문합술을 실시하였다. 술 후 한달 째 배뇨장애나 혈뇨 증상이 없이, 정상적인 배뇨를 유지하였으며, 수술 후 2 달째 실시한 역행성 양성 요도 조영술에서 요도 협착 소견을 보이지 않았다.