• Title/Summary/Keyword: BmN4

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Can bone marrow aspirate improve mandibular fracture repair in camels (Camelus dromedarius)? A preliminary study

  • Al-Sobayil, Fahd;Sadan, Madeh A.;El-Shafaey, El-Sayed;Ahmed, Ahmed F.
    • Journal of Veterinary Science
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    • v.21 no.6
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    • pp.90.1-90.11
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    • 2020
  • Background: Mandibular fractures are common in camels, leading to considerable economic losses. This study explored methods of improving mandibular fractures repair, adjuvant with interdental wire, or bone plate fixation. Autologous bone marrow (BM) injection enhances osteogenesis and rapid healing. Objectives: To investigate the effect of autologous BM aspirate as an adjuvant treatment for repairing mandibular fractures in camels with interdental wire, or bone plate fixation. Methods: Thirty dromedary camels aged 5-8 years and of both sexes were randomly divided into 4 treatment groups: group 1 (n = 10) treated with stainless steel wire fixation and BM injection at the fracture line, group 2 (n = 10) treated with plate fixation and BM injection at the fracture line, group 3 (n = 5) treated with stainless steel bone wire fixation and placebo saline injection at the fracture line, and group 4 (n = 5) treated with plate fixation and placebo injection at the fracture line. The mandibular fractures were followed weekly for 12 weeks postoperatively to assess improvement and healing based on clinical evaluation, radiographic union scale, and bone turnover markers (i.e., bone alkaline phosphatase, osteocalcin, pyridinoline, and deoxypyridinoline). Results: Compared to other groups, elevated bone turnover markers in group 1 were demonstrated (p < 0.05) on the seventh postoperative day. Likewise, compared to other groups, both clinical findings and radiographic union scale significantly improved (p < 0.05) in group 1 on the 56th postoperative day. Conclusions: BM aspirate has a promising beneficial osteogenic effect on mandibular fracture repair in camels, most notably when combined with interdental wire fixation.

Robust Quick String Matching Algorithm for Network Security (네트워크 보안을 위한 강력한 문자열 매칭 알고리즘)

  • Lee, Jong Woock;Park, Chan Kil
    • Journal of Korea Society of Digital Industry and Information Management
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    • v.9 no.4
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    • pp.135-141
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    • 2013
  • String matching is one of the key algorithms in network security and many areas could be benefit from a faster string matching algorithm. Based on the most efficient string matching algorithm in sual applications, the Boyer-Moore (BM) algorithm, a novel algorithm called RQS is proposed. RQS utilizes an improved bad character heuristic to achieve bigger shift value area and an enhanced good suffix heuristic to dramatically improve the worst case performance. The two heuristics combined with a novel determinant condition to switch between them enable RQS achieve a higher performance than BM both under normal and worst case situation. The experimental results reveal that RQS appears efficient than BM many times in worst case, and the longer the pattern, the bigger the performance improvement. The performance of RQS is 7.57~36.34% higher than BM in English text searching, 16.26~26.18% higher than BM in uniformly random text searching, and 9.77% higher than BM in the real world Snort pattern set searching.

Biogenesis of Lysosome-related Organelle Mutant Silkworms by Direct Injection of a Cas9 Protein-guided RNA Complex into Bombyx mori Embryos (Cas9 단백질/ 가이드 RNA 복합체를 이용한 누에 BmBLOS 유전자 편집)

  • Kim, Kee Young;Yu, Jeong Hee;Kim, Su-Bae;Kim, Seong-Wan;Kim, Seong-Ryul;Choi, Kwang-Ho;Kim, Jong Gil;Park, Jong Woo
    • Journal of Life Science
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    • v.29 no.5
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    • pp.537-544
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    • 2019
  • Genome editing technology employing gene scissors has generated interest in molecular breeding in various fields, and the development of the third-generation gene scissors of the clustered, regularly interspaced short palindromic repeat (CRISPR) system has accelerated the field of molecular breeding through genome editing. In this study, we analyzed the possibility of silkworm molecular breeding using gene scissors by genomic and phenotypic analysis after editing the biogenesis of lysosome-related organelles (BmBLOS) gene of Bakokjam using the CRISPR/Cas9 system. Three types of guide RNAs (gRNA) were synthesized based on the BmBLOS gene sequence of Bakokjam. Complexes of the prepared gRNA and Cas9 protein were formed and introduced into Bombyx mori BM-N cells by electroporation. Analysis of the gene editing efficiency by T7 endonuclease I analysis revealed that the B4N gRNA showed the best efficiency. The silkworm genome was edited by microinjecting the Cas9/B4N gRNA complex into silkworm early embryos and raising the silkworms after hatching. The hatching rate was as low as 18%, but the incidence of mutation was over 40%. In addition, phenotypic changes were observed in about 70% of the G0 generation silkworms. Sequence analysis showed that the BmBLOS gene appeared to be a heterozygote carrying the wild-type and mutation in most individuals, and the genotype of the BmBLOS gene was also different in all individuals. These results suggest that although the possibility of silkworm molecular breeding using the CRISPR/Cas9 system would be very high, continued research on breeding and screening methods will be necessary to improve gene editing efficiency and to obtain homozygotes.

Synthesis and Photovoltaic Properties of Conducting Polymers Based on Phenothiazine (Phenothiazine계 전도성고분자의 합성 및 유기박막태양전지로의 적용 연구)

  • Yoo, Han-Sol;Park, Yong-Sung
    • Applied Chemistry for Engineering
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    • v.24 no.1
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    • pp.93-98
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    • 2013
  • In this paper, four conducting polymers (poly[(N-butyl-phenothiazine)-sulfide] (PBPS), poly[(N-hexyl-phenothiazine)-sulfide] (PHPS), poly[(N-decyl-phenothiazine)-sulfide] (PDPS), and poly[(N-(2-ethylhexyl)-phenothiazine)-sulfide] (PEHPS)) were synthesized with a high temperature and high pressure reaction. The structures of synthesized polymers were confirmed by $^1H-NMR$ and characterized by UV-Vis, cyclic voltammetry, and GPC. From the UV-Vis absorption spectra, the ${\lambda}_{max}$ values of PBPS, PHPS, PDPS, and PEHPS were 338, 341, 340, and 334 nm, respectively and their optical band gaps were 3.11, 3.13, 3.16, and 3.05 eV, respectively. To evaluate the feasible applicability as a photovoltaic cell, the devices composed of for example, ITO/PEDOT : PSS/polymer (PBPS, PDPS) : $PC_{71}BM$ (1 : 3, w/w)/$BaF_2$/Ba/Al were fabricated using the blends of the PBPS and PDPS as a donor, and $PC_{71}BM$ as an acceptor. Then, the power conversion efficiencies (PCE) of devices were estimated as 0.076% of PBPS and 0.136% of PDPS by solar simulator.

A Study on Design of Reflector Type Frequency Doubler in K-Band (리플렉터 형태의 K-대역 주파수 체배기 구현에 관한 연구)

  • Han, Sok-Kyun;Choi, Hyung-Ha
    • Journal of Navigation and Port Research
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    • v.28 no.1
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    • pp.37-41
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    • 2004
  • In this paper, a reflector type frequency doubler for local oscillator at 24GHz is designed and fabricated with ne71300-N MESFET. Optimum source and load impedances are decided through a multiharmonic load pull simulation technique. A conversion gain can be improved using the reflector and fundamental and third harmonics are well suppressed with open stub of $\lambda$/4 length Measured results show output power at 0dBm of input power is -3.776dBm, conversion gain 0.736dB, harmonic suppression 41.064dBc, respectively.

A study on the fatigue and fracture characteristics of localized nuclear reactor vessel material (국산 원자로용기 재료의 피로 및 파괴특성 연구)

  • Jeong, Sun-Eok
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.21 no.10
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    • pp.1626-1635
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    • 1997
  • It is important to ensure the reliability of the first localized reactor vessel steel. To satisfy with this purpose, a study on the impact/hardness, low cycle fatigue(LCF), crack growth rate(da/dN) and fracture toughness( ) of base material(BM) and weld metal(WM) were performed under room temperature air and corrosion conditions. A summary of the results is as folows : (1) Charpy impact absorbed energy of BM was the highest value, heat affected zoon(HAZ) and the lowest, WM. The hardness of BM was similar to HAZ. (2) Coefficients of Manson equation using the monotonic tensile test data were obtained for the present material. (3) The effects of stress ratio and ambient (120.deg. C and NaCl) condition on da/dN were investigated, da/dN with NaCl condition expressed the highest value. (4) The results of Charpy V-notch impact test had good correlation with $K_{IC}$ characteristics and the lowest curve of $K_{IC}$ for BM was derived, more researches about WM and HAZ are required hereafter.

Molecular Biological Characterization of Recombinant Baculovirus with an Expanded Host Range (숙주범위가 넓어진 유전자 재조합 핵다각체병 바이러스의 분자생물학적 특성)

  • 김우진;우수동
    • Journal of Sericultural and Entomological Science
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    • v.38 no.1
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    • pp.42-47
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    • 1996
  • To investigate the host range determining factors of nuclear polyhedrois virus (NPV), Autographa california NPV and Bombyx mori NPV were coinfected into the two different cell lines, BmN-4 and Sf-9. The recombinant baculoviruses, RecS-A6 and RecB-727 which have an expanded host range, were isolated from Sf-9 and BmN-4 cell lines, respectively. The molecular biological characteristics of the recombinant baculoviruses were investigated. The pathogenicity of RecB-727 was similar to that of wild type BmNPV, while the pathogenicity of RecS-A6 was relatively lower than that of wild type BmNPV. The restriction enzyme digestion patterns of parental viruses and recombinant viruses showed that the recombinant virus has an expanded host range by genetic recombination. Southern blot analysis revealed that the p10 gene of RecB-727 was derived from AcNPV genomic DNA, while RecS-A6 has p10 gene of BmNPV in a viral genome. To investigate the host range expansion mechanism of recombinant baculovirus, HindIII-SacI 0.6 kb DNA fragments of RecS-A6 and RecB-727 were cloned and sequenced. The results showed that of wild type BmNPV helicase gene, suggesting that the expanded host range of recombinant baculoviruses was due to the insertion of BmNPV helicase gene into AcNPV viral genome.

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Study on the Corrosion Charactristics and its Corrosion Protection of Steel Fishing Banks (강제어촌의 부식특성과 그 방식에 관한 연구)

  • Lim, Uh-Joh;Lee, Jong-Rark
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.28 no.2
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    • pp.216-227
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    • 1992
  • The corrosion rate, behavior of corrosion fatigue and characteristic of cathodic protection for SB41 were investigated by corrosion and corrosion control tests in seawater at laboratory and coast. The main result obtained are as the following; 1) The corrosion rate of base metal (BM) is about 28-37 mg/dm super(2) day in seawater of coast. 2) The correlation between the stress intensity factor range $\Delta$K and crack propagation rate da/dN for weldment follows paris' rule in seawater : da/dN=C($\Delta$K) super(m) where m is the slope of the correlation, and is 2.02 for BM and 1.75 for heat affected zone (HAZ) respectively. 3) The corrosion sensitivity of HAZ is more sensitive than that of BM under the low region of $\Delta$K. 4) With increase of bared surace area of cathode, cathodic protection potential is increased sharply.

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High Efficiency GaN HEMT Power Amplifier Using Harmonic Matching Technique (고조파 정합 기법을 이용한 고효율 GaN HEMT 전력 증폭기)

  • Jin, Tae-Hoon;Kwon, Tae-Yeop;Jeong, Jinho
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.25 no.1
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    • pp.53-61
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    • 2014
  • In this paper, we present the design, fabrication and measurement of high efficiency GaN HEMT power amplifier using harmonic matching technique. In order to achieve high efficiency, harmonic load-pull simulation is performed, that is, the optimum load impedances are determined at $2^{nd}$ and $3^{rd}$ harmonic frequencies as well as at the fundamental. Then, the output matching circuit is designed based on harmonic load-pull simulation. The measurement of the fabricated power amplifier shows the linear gain of 20 dB and $P_{1dB}$(1 dB gain compression point) of 33.7 dBm at 1.85 GHz. The maximum power added efficiency(PAE) of 80.9 % is achieved at the output power of 38.6 dBm, which belongs to best efficiency performance among the reported high efficiency power amplifiers. For W-CDMA input signal, the power amplifier shows a PAE of 27.8 % at the average output power of 28.4 dBm, where an ACLR (Adjacent Channel Leakage Ratio) is measured to be -38.8 dBc. Digital predistortion using polynomial fitting was implemented to linearize the power amplifiers, which allowed about 6.2 dB improvement of an ACLR performance.

Study on the Corrosion Fatigue Crack Propagation Behavior of Steel Used for Frame of Vehicles in Marine Environment (해양환경중에서 자동차 프레임용 강재의 부식피로균열전파거동에 관한 연구)

  • 이상열;임종문;이종악
    • Journal of Ocean Engineering and Technology
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    • v.6 no.2
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    • pp.76-84
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    • 1992
  • In this study, corrosion fatigue test of SAPH45 steel was performed by the use of plane behavior of base metal (BM) and heat affected zone (HAZ) of SAPH45. The main results obtained are as follows: 1) The more aspect ratio (b/t) of corner crack decreases, the more aspect ratio (b/a) takes greatly effect by corrosion. 2) The correlation between the stress intensity factor range ($\Delta$k) and crack growth rate (da/dN) for weldment in seawater is given by Paris rule as follow: da/dN=C($\Delta$K) super(m). Where m is constant, and the value is 3.82-3.84. 3) The accelerative factor ($\alpha$) of BM and HAZ under seawater is about 1.1-1.9, and ($\alpha$) of HAZ increases more and more under the low $\Delta$K region. 4) HAZ is more susceptible to corrowion than BM because of potential of electrode (E sub(c)) of HAZ becomes more less noble potential than that of BM.

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