• Title/Summary/Keyword: BmA3

Search Result 1,000, Processing Time 0.03 seconds

Expression of Human Stem Cell Factor with Recombinant Baculovirus in BmN Cell Line and Silkworm

  • Xijie, Guo;Yongfeng, Jin;Mingguan, Yang;Yaozhou, Zhang
    • International Journal of Industrial Entomology and Biomaterials
    • /
    • v.4 no.1
    • /
    • pp.51-56
    • /
    • 2002
  • A recombinant transfer vector pBacSCF was constructed by inserting huamn stem cell factor (hSCF) cDNA into plasmid pBacPAK8. BmN cells were co-transfected with modified Bombyx mori, nuclear polyhedrosis virus (BmBacPAK) DNA and the recmbinant transfer vector to construct a recombinant baculovirus containing hSCE gene. DNA dot blotting and RNA dot blotting demonstrated that the hSCE gene was contained in the recombinant virus and transcribed. The recombinant baculovirus was infectious to BmN cells and to silkworm. SDS-PAGE analysis showed a specific band of expressed product in the extract of infected cells and in the heamolymph of infected larvae. Bioactivity of the recombinant hSCE was determined with W-1 cell line and MTT colorimetric method in synergy with interlukin-3 (IL-3). These results revealed that the hSCF gene was over-expressed in cultured cells and lavae of silkworm.

EVALUATION OF THE CONVOLUTION SUMS Σak+bl+cm=n σ(k)σ(l)σ(m), Σal+bm=n lσ(l)σ(m) AND Σal+bm=n σ3(l)σ(m) FOR DIVISORS a, b, c OF 10

  • PARK, YOON KYUNG
    • Journal of applied mathematics & informatics
    • /
    • v.40 no.5_6
    • /
    • pp.813-830
    • /
    • 2022
  • The generating functions of the divisor function σs(n) = Σ0<d|n ds are quasimodular forms. In this paper, we find the basis of the space of quasimodular forms of weight 6 on Γ0(10) consisting of Eisenstein series and η-quotients. Then we evaluate the convolution sum Σak+bl+cm=n σ(k)σ(l)σ(m) with lcm(a, b, c) = 10 and Σal+bm=n lσ(l)σ(m) and Σal+bm=n σ3(l)σ(m) with lcm(a, b) = 10.

High-Speed Low-Complexity Reed-Solomon Decoder using Pipelined Berlekamp-Massey Algorithm and Its Folded Architecture

  • Park, Jeong-In;Lee, Ki-Hoon;Choi, Chang-Seok;Lee, Han-Ho
    • JSTS:Journal of Semiconductor Technology and Science
    • /
    • v.10 no.3
    • /
    • pp.193-202
    • /
    • 2010
  • This paper presents a high-speed low-complexity pipelined Reed-Solomon (RS) (255,239) decoder using pipelined reformulated inversionless Berlekamp-Massey (pRiBM) algorithm and its folded version (PF-RiBM). Also, this paper offers efficient pipelining and folding technique of the RS decoders. This architecture uses pipelined Galois-Field (GF) multipliers in the syndrome computation block, key equation solver (KES) block, Forney block, Chien search block and error correction block to enhance the clock frequency. A high-speed pipelined RS decoder based on the pRiBM algorithm and its folded version have been designed and implemented with 90-nm CMOS technology in a supply voltage of 1.1 V. The proposed RS(255,239) decoder operates at a clock frequency of 700 MHz using the pRiBM architecture and also operates at a clock frequency of 750 MHz using the PF-RiBM, respectively. The proposed architectures feature high clock frequency and low-complexity.

Enhanced Production of Chlorella ovalis and Dunaliella parva by the Rates of Medium Composition Obtained from the Fermented Animal Wastewater Including a Natural Substitute Chelator for EDTA (EDTA 대체용 천연 킬레이팅제를 함유한 발효 축산폐수의 배지조성률에 따른Chlorella ovalis와 Dunaliella parva의 생산성 강화)

  • Jeon, Seon-Mi;Jeune, Kyung-Hee;Kim, Mi-Kyung
    • ALGAE
    • /
    • v.21 no.3
    • /
    • pp.333-341
    • /
    • 2006
  • The productivities of Chlorella ovalis and Dunaliella parva were influenced by the rates of medium compositions obtained from the fermented animal wastewater (BM: bacteria mineral water) including a natural substitute chelator for EDTA (etylenediaminetetraacetic acid). The most favorable medium was -E+50 adding 50% BM in f/2 medium instead of EDTA, a chemical chelator, which increased more 19-fold of cell density in C. ovalis and 7-fold in D. parva than cells cultured on f/2 medium as well as the enhancements of chlorophyll a (f/2-E: 0.26 g L–1, -E+50: 1.5 g L–1 in C. ovalis; f/2-E: 2.7 g L–1, -E+50: 15 g L–1 in D. parva) and the increase of maximal PSII quantum yields. These results were verified that the BM could play an important part as a natural chelator substituted for EDTA. In the fields of biotechnology, food organisms in fishery and eco-industries of CO2 sequestration in air and nutrient removal in water, the natural chelator of BM could be applied to enhance the biomass of the other microalgae.

Retrograde Analysis of Clinical Characteristics of Bone Metastasis in 1,031 Cases of Preliminarily Diagnosed Nasopharyngeal Carcinoma

  • Zhao, Chang-Lin;Qian, Guo-Qiang;Chen, Xiao-Yin;Chen, Chao
    • Asian Pacific Journal of Cancer Prevention
    • /
    • v.15 no.8
    • /
    • pp.3785-3788
    • /
    • 2014
  • Purpose: To explore the clinical characteristics of bone metastasis (BM) in a large sample of preliminarily diagnosed nasopharyngeal carcinomas (NPCs). Methods: The sample consisted of 1,031 patients diagnosed with NPC at first visitg clinics between October 1989 and June 2012. Several parameters including metastasis locus, T/N staging, diagnosis, therapy and prognosis of BM were analyzed retrospectively. Results: In 70 patients who had been preliminarily diagnosed with BM, the incidence of BM in N0, N1, N2 and N3 stage was 5.7%, 17.2%, 50.2%, and 25.7%, respectively, while the incidence in T0, T1, T2 and T3 stage was 0%, 23.8%, 47.6% and 28.6% respectively. BM occurred in most common in vertebral column, rib, sternum, ilium and femur. Positive rate of Epstein-Barr virus antibody was 77.6%. The median survival time was 12 months. Conclusion: The incidence of BM in NPC preliminarily diagnosed is about 7% and it is related to N classification but not T classification.

Expression of the FLP recombinase of the 2 $\mu$m plasmid of yeast in the cultured cells of Bombyx mori using a transient expression vector (Yeast 2 $\mu$m 플라스미드 유래 FLP recombinase 유전자의 곤충 배양세포내 발현)

  • 강석우;윤은영
    • Journal of Sericultural and Entomological Science
    • /
    • v.39 no.1
    • /
    • pp.36-43
    • /
    • 1997
  • In order to express the FLP recombinase in B. mori cultured cell line, BmN-4, transient expression system using a heat shock protein gene (hsp70) promoter of Dorosophilla melnogaster was constructed. This vector was designated as pHsSV. Activity strength of the hsp70 promoter was compared with that of immediate early gene (IE-1) and polyhedrin gene of BmNPV employing the E. coli $\beta$-galactosidase gene as a reporter gene. The result showed that the pHs $\beta$-gal plasmid vector expressed the $\beta$-galactosidase at 2nd and 3rd day after the transfer of plasmid DNA into BmN-4 cells, which was similar to that of pIE1 $\beta$-gal vector, but different from that of a recombinant virus, vBm $\beta$-gal. For the construction of FLP recombinase transient expression vector, the FLP recombinase gene was cloned by polymerase chain reaction technique. To express the FLP recombinase, this gene was inserted into pHsSV plasmid vector, under the control of the hsp70 promotor, and tranfected in BmN-4 cells. The expressed FLP recombinase was estimated at 44kDa on a 12.5% SDS-PAGE.

  • PDF

Effect of 12-week Low Calorie Diet and Behavior Modification on the Anthropomeric Indices and Biochemical Nutritional Status of Obese Woman (12주 동안의 저열량식사와 행동수정요법이 비만여성의 체격지수와 생화학적 영양상태에 미치는 영향)

  • Son, Sook-Mee;Kim, Hee-Jun
    • Korean Journal of Community Nutrition
    • /
    • v.10 no.4
    • /
    • pp.525-535
    • /
    • 2005
  • This study was conducted to investigate the effect of a 3 week low calorie diet (LCD) and a 9 week of behavior modification (BM) program on the weight loss, mineral and vitamin status in 22 obese women. The subject were healthy, obese (PIBW> $120\%$) women aged 20 - 50 Yr and not taking any medications known to influence body composition, mineral or vitamin metabolism During the LCD program, subjects were provided commercial liquid formulas with 125 kcal per pack and were instructed to have a formula for replacement of one meal and at least one regular meal per day within the range of daily 800 - 1200 kcal intake. During the BM program the subjects weekly attended the group nutrition counseling session to encourage themselves to modify their eating behavior and spontaneously restrict their energy intakes. The BM program focused on stimulus control, control of portion sizes and modification of binge eating and other adverse habits. The initial mean energy intake of subjects was 2016.9 $\pm$ 129.8 kcal ($100.8\%$ of RDA) and dropped to 1276.5 $\pm$ 435.7 kcal at the end of a 3 week of LCD program and elevated to 1762 $\pm$ 329.3 kcal at the end of a 9 week of BM program. Carbohydrate, protein and fat intakes were significantly decreased at the end of the LCD but carbohydrate was the only macro nutrient that showed significant decrease (p < 0.05) at the end of the BM program compared to baseline. Calcium and iron intakes decreased significantly (p < 0.01, respectively) with no significant changes in other micronutrients at the end of the LCD. The mean weight of the subjects decreased from 73.8 $\pm$ 8.0 kg to 69.2 $\pm$ 7.7 kg with LCD and ended up with 67.7 $\pm$ 7.1 kg after 9 weeks of BM. The 3 weeks of LCD reduced most of the anthropometric indices such as BMI, PIBW, fat weight, wast-to-hip ratio and subscapular and suprailiac skinfold thickness. The 9 weeks of behavior modification showed slight change or maintenance of each anthropometric measurements. Weight loss and decreased WHR with the diet program induced significantly decreased systolic blood pressure. SGOT, SGPT and serum insulin levels with improved serum lipid profiles. Biochemical parameters related to iron status such as hemoglobin, hematocrit were significantly decreased (p < 0.01) at the end of the LCD. But their mean values were within normal range. The mean serum 25 (OH) vitamin $D_3$ level significantly increased after whole diet program. Serum folate level significantly decreased after 12 weeks of diet program. In conclusion 3 weeks of LCD brought 4.6 kg reduction in body weight without risk of iron, zinc or vitamin D deficiency and 9 weeks of the BM was effective to maintain nutritional status with slightly more weight reduction (1.5 kg). However calcium intake and serum folate should be monitored during the LCD and BM because of increased risk of deficiencies.

The innate immune response transcription factor Bombyx mori Relish1 induces high-level antimicrobial peptides in silkworm

  • Kim, Seong-Wan;Kim, Seong-Ryul;Goo, Tae-Won;Choi, Kwang-Ho
    • International Journal of Industrial Entomology and Biomaterials
    • /
    • v.37 no.2
    • /
    • pp.49-54
    • /
    • 2018
  • To artificially enhance antimicrobial peptide expression in Bombyx mori, we constructed genetically engineered silkworms overexpressing Rel family transcription factor. The truncated BmRelish1 (BmRelish1t) gene contained a Rel homolog domain (RHD), nuclear localization signal (NLS), acidic and hydrophobic amino acid (AHAA)-rich region, and death domain (DD), but no ankyrin-repeat (ANK) domain. The BmRelish1t gene was controlled by B. mori cytoplasmic actin 3 promoter in the PiggyBac transposon vector. Chromosome analysis of G1 generations of a transgenic silkworm with EGFP expression confirmed stable insertion of BmRelish1t. BmRelish1t gene overexpression in transgenic silkworms resulted in higher mRNA expression levels of B. mori antimicrobial peptides such as lebocin(~20.5-fold), moricin(~8.7-fold), and nuecin(~17.4-fold) than those in normal silkworms.

Synthesis and Photovoltaic Properties of a Low Band Gap Polymer for Organic Solar Cell (유기태양전지를 위한 작은 밴드갭 고분자의 합성과 광전특성)

  • Woo, Yong-Ho;Lee, Hyo-Sang;Park, Sungnam;Choi, E-Joon;Kim, BongSoo
    • Polymer(Korea)
    • /
    • v.39 no.1
    • /
    • pp.71-77
    • /
    • 2015
  • We synthesized a low band gap alternating copolymer containing electron-rich units (i.e. dithienosiloles and benzodithiophenes) and electron-deficient units (i.e. difluorobenzothiadiazoles) for high performance organic solar cells. The polymer was prepared by the Stille coupling reaction and characterized using $^1H$ NMR, GPC, TGA, UV-visible absorption spectroscopy, and cyclic voltammetry. Solar cells were fabricated in a structure of ITO/PEDOT:PSS/polymer: $PC_{70}BM/Al$ with five different blending ratios of polymer and $PC_{70}BM$ (1:1.5, 1:2, 1:3, 1:3.5 and 1:4 by weight ratio). The best efficiency was achieved from the 1:3 ratio of polymer and $PC_{70}BM$ in the photoactive layer, and TEM revealed that there is an optimal nanoscale phase separation between polymer and $PC_{70}BM$ in the 1:3 ratio blend film.

A Detection Algorithm Study of the Victim Signal for the DAA Regulation in MB-OFDM UWB System (MB-OFDM UWB 시스템에서 DAA 기술 기준 적용을 위한 피 간섭 신호 검출 방안 연구)

  • Shin, Cheol-Ho;Choi, Sang-Sung
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
    • /
    • v.20 no.12
    • /
    • pp.1297-1307
    • /
    • 2009
  • The purpose of this paper is to propose a detection algorithm and a tracking algorithm based on silent time using MB-OFDM UWB(Multi-Band Orthogonal Frequency Division Multiplexing Ultra Wide Band) receiver in order to satisfy DAA(Detect And Avoid) regulation of Korea to permit UWB in 3.1~4.8 GHz. In DAA regulation of Korea, if UWB device receives a signal more than -80 dBm/MHz from the victim system during UWB operation, the UWB system should avoid the collision within 2 sec. In this paper, we proposed the detection algorithm to detect the victim signal received by -80 dBm/MHz for the avoidance process that changes the operating UWB frequency to other UWB frequency and the subcarrier tracking algorithm to follow up the subcarrier positions of the victim signal for the tonenulling avoidance process that decreases the TX power of subcarriers occupied by the victim signal by -70 dBm/MHz. The performance of the detection algorithm and the tracking algorithm suggested in this paper is verified in simulation results considering various conditions.