• Research in Plant Disease
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• v.13 no.2
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• pp.88-92
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• 2007

A total of 41 Pseudomonas syringae pv. syringae, the causal agent of bacterial blossom blight, were isolated from kiwifruit plants in Korea. Among them, two strains showing streptomycin resistance were examined to investigate the structure of resistant determinants by PCR and nucleotide sequence analysis. PCR results suggested that the streptomycin resistance is mediated by strA-strB genes carried on Tn5393a. Insertion sequences, IS6100 and IS1133, which were located within or downstream of tnpR gene in Xanthomonas campestris and Erwinia amylovora were not found. Nucleotide sequences of strA-strB were 100% identical with Tn5393a. Two stretomycin resistant strains had three plasmids. Southern blot hybridization using strA-strB probe indicated that the resistant genes were carried on a 100kb plasmid.

• Journal of Periodontal and Implant Science
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• v.27 no.4
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• pp.867-882
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• 1997

Safflower(Carthamus tinctorius $Linn\acute{e}$ has been traditionally used for the treatment of blood stasis, and Dipsasi Radix has been used as a drug for fracture in Chinese medicine. The purpose of present study was to examine the biologic effects of safflower extract and Disasi radix extracts on the periodontal. ligament cells and osteoblastic cells and on the wound healing of rat calvarial defect. The ethanolic extract of safflower blossom, safflower seed and Dipsasi Radix(125, 250, and 500 ${\mu}g/ml$) were prepared as test group, and PDGF-BB(lOng/ml) and unsafonifiable fraction of Zea Mays L.(125, 250, and 500 ${\mu}g/ml$) were employed as positive control. The effects of each agents on the growth and survival, ALPase activity, expression of PDGF-BB receptor, chemotactic response of PDL cell and ATCC human osteosarcoma MG63 cells in vitro were examined. The tissue regenerative effect of each extracts was evaluated by histomorphometric measuring of newly formed bone on the 8mm defect in rat calvaria after oral administration of 3 different dosages groups : 0.02, 0.1 and 0.35g/kg, per day. It was also employed the same dosages of unsaponifiable fraction of Zea Mays L. as positive controls. Safflower blossom extract, safflower seed extract, and Dipsasi Radix extract stimulate the cellular activity of MG63 cells in concentration range of $125-500{\mu}g/ml$, and safflower bolssom extract and safflower seed extract stimulate also the cellular activity of periodontal ligament cells in concentration range of $250-500{\mu}g/ml$. In activity of ALPase, $250-500{\mu}g/ml$ of safflower blossom extracts showed significant stimulating effects on MG63 cells, and the same concentration range of safflower seed extracts showed significant effect on periodontal ligament cells. In the recovery on PDGF-BB receptor expression which was depressed by $IL-1{\beta}$, $125-250{\mu}g/ml$ of safflower blossom extracts and $250-500{\mu}g/ml$ of safflower seed extracts showed significant increasing effect on MG63 cells, and $500{\mu}g/ml$ of safflower blossom extract and $250-500{\mu}g/ml$ of safflower seed extracts showed significant effect on periodontal ligament cells. In chemotactic response, among all tested group, safflower seed extracts only were chemotactic to MG63 cells and periodontal ligament cells in concentration range of $125-500{\mu}g/ml$. Also in the view of bone regeneration in rat calvarial defect model, the only group that was orally administrated 0.35g/kg, day of safflower seed extract showed significant new bone formation. These results suggested that safflower extracts might have a potential possibilities as an useful drug for adjunct to treatment for regeneration of periodontal defect.

• Journal of Environmental Science International
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• v.23 no.5
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• pp.781-786
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• 2014

Growth and physiological disorders caused by abnormally low temperatures were evaluated in pepper, an important field crop in Korea. In addition, the effects of chemical treatment using glutamine was verified on minimizing the damages by low temperature. The growth of pepper plants in stem length and diameter was suppressed as the temperature decreased from $25^{\circ}C$, and the suppression level was the highest for plants grown for 90 days at $20^{\circ}C$. However, root growth was not affected by the different temperatures. The number of leaf and leaf area decreased at the temperatures below $25^{\circ}C$, an optimum temperature for growth. Fresh weight and dry weight decreased for plants grown at $20^{\circ}C$. Pepper fruit yield also decreased by 11% at $20^{\circ}C$ in comparison to $25^{\circ}C$. Falling blossom rate was different depending on the growth temperature, and the rate was 27.2% at $25^{\circ}C$, 35.2% at $22.5^{\circ}C$, and 41.0% at $20^{\circ}C$, indicating that falling blossom rate increased as temperature decreased. Different growth temperatures did not affected on the level of symptom of calcium deficiency and Phytopathora blight. Falling blossom was severe at abnormally low temperature of $20^{\circ}C$, but the treatment of glutamine reduced falling blossom rate and increased the yield by 7.0% as compared to control. The optimum concentration of glutamine treatment was 10 mg/L for yields.

• Horticultural Science & Technology
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• v.33 no.4
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• pp.486-491
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• 2015

In this study, we evaluated the differences in fruit development and quality indices of fruits subjected to blossom thinning before full bloom in 'Hanareum' and 'Niitaka' pears (Pyrus pyrifolia Nakai). We carried out a thinning treatments at 5 days before full bloom, targeting the blossoms at the 1st and 2nd position (T1) or the blossoms at the 6th and 7th position (T2) from the basal part of the flower cluster in both cultivars. Blossom thinning treatments increased the average fruit weight of early-season Asian pear 'Hanareum' up to 10.5% (T1) and 11.0% (T2) at harvest time (110 days after full bloom). The mid-season 'Niitaka' pear showed increased fruit weight of up to 12.6% (T1) and 16.8% (T2) at harvest time (170 days after full bloom). Moreover, these treatments increased the production rate of bigger sized fruits in both cultivars. Removal of blooms also affected fruit quality indices; the bloom-thinned fruits showed higher soluble solids contents (about $1^{\circ}Brix$) and higher skin color redness ($a^*$) especially in 'Niitaka' pears when compared to the non-thinned controls. Overall, the treatments increased the fruit size and weight as well as fruit quality including soluble solids and skin color. Bloom thinning treatment targeting the 6th to 7th blossom from the basal part of the flower cluster may be recommended as the practical thinning method in Asian pear 'Hanareum' and 'Niitaka'.

• Korean Journal of Environmental Agriculture
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• v.39 no.4
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• pp.360-367
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• 2020

BACKGROUND: The productivity and quality of blueberry (Vaccinium ashei Reade) greatly depend on the number of fruits in a plant. Especially, fruit set more than appropriate number negatively affects productivity and marketability due to the increased number of small fruits and delayed harvest time. This study was conducted to investigate proper timing and concentration for applying chemical blossom thinners such as ammonium thiosulfate (ATS) and UREA. METHODS AND RESULTS: ATS at 1.25% and 1.50%, and UREA at 6% and 8% were applied in four developmental stages, bud swell, pink bud, full bloom and petal fall. Fruit thinning rate was calculated based on the number of fruits harvested divided by that of flowers before applying blossom thinners. Ratios of leaf to flower and leaf to fruit were calculated based on the number of fully developed leaves in 25 days after full blossom divided by that of flowers or fruits, respectively. Chemical injury of leaves was investigated by calculating the number of leaves with chemical injury divided by the total number of leaves. Fruit thinning rates were 48% and 66% for UREA treatments at 6% and 8%, respectively, and 49% and 62% for ATS treatments at 1.25% and 1.50%, respectively, in the full bloom stage. In the petal fall stage, fruit thinning rates were 18% and 24% for UREA treatments at 6% and 8%, respectively, and 49% and 35% for ATS treatments at 1.25% and 1.50%, respectively. Leaf to fruit ratio (L/FR) increased by 109% and 188% compared to leaf to flower ratio in ATS treatments at 1.25% and 1.50%, respectively, and L/FR increased 93 and 196% in UREA treatments at 6% and 8%, respectively, in the full bloom stage. In the petal fall stage, leaf to fruit ratio increased by 60% to 100% in ATS treatments, but did not significantly differ from the control in UREA treatments. Fruit harvest was delayed in all treatments of all developmental stages except for 1.5% ATS and 6% UREA treatments at the petal fall stage, whose fruit harvest was two or three days faster than the control. CONCLUSION: The application of ATS and UREA for blossom thinning should be in the petal fall and full bloom stages for early and late harvest, respectively. Considering chemical injury, integrated harvesting and fruit size, however, it is appropriate to apply ATS at 1.5% in the petal fall stage to increase fruit productivity and quality in blueberry.

• Korean Journal of Agricultural and Forest Meteorology
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• v.24 no.4
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• pp.305-317
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• 2022

To investigate the blossom infection risk of fire blight on pears, the program Maryblyt has been executed from 2018 to 2022 based on meteorological data from central-Korean cities where fire blight has occurred as well as from southern Korean cities where the disease has not yet occurred. In the past five years, years with the highest risk of pear blossom blight were 2022 and 2019. To identify the optimal time for spraying, we studied the spray mode according to the Maryblyt model and recommend spraying streptomycin on the day after a "High" warning and then one day before forecasted precipitation during the blossom period. Maryblyt also recommends to initiate surgical controls from mid-May for canker blight symptoms on pear trees owing to over-wintering canker in Korea. Web-cam pictures from pear orchards at Cheonan, Icheon, Sangju, and Naju during the flowering period of pear trees were used for comparing real data and constructing a phenological model. The actual starting dates of flowering at southern cities such as Sangju and Naju were consistently earlier than those calculated by the model. It is thus necessary to improve the forecasting model to include field risks by recording the actual flowering period and the first day of the fire blight symptoms, according to the farmers, as well as mist or dew-fall, which are not easily identifiable from meteorological records.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 1999.10a
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• pp.62.2-62
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• 1999

• Journal of Bio-Environment Control
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• v.13 no.4
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• pp.194-199
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• 2004

To investigate effect of soil conditioners on salt reducing effect, saline soil were treated with photomicrobe, soil plus, pyroligneous liquor and active charcoal and cucumber and tomato were grown. Yield and root development were enhanced in active charcoal treatment overall and tomato blossom end rot frequency was lowest in active charcoal treatment. Total sugar content was highest in soil plus treatment and nitrate and phosphate content were not significantly different within 4 treatments.

• Korean Journal of Agricultural Science
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• v.47 no.1
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• pp.67-81
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• 2020

The physicochemical parameters of honey are used to determine the botanic origin of honey and to specify the composition criteria for honey in regulations and standards. The parameters of honeydew and blossom honeys from Korean beekeepers were determined to investigate whether they complied with the composition criteria for honey in the food code legislated by Korean authority and to establish the parameters which should be subjected to principal component analysis for improved differentiation of honeys. The fructose and glucose contents of the honeydew honey did not comply with the composition criteria. The ash content of the honey was closely correlated with CIE a^* and CIE L^* The principal component analysis of fructose to glucose ratio, CIE a^*, CIE L^*, ash content, free acidity, and fructose and glucose contents enabled classification of honeydew, chestnut, multifloral, and acacia honeys. Additional advantage of the principal component analysis (PCA) is that the physicochemical parameters, such as fructose to glucose ratio (F/G) and color, can be determined using the analytical instruments for composition criteria and quality control of honey. This study suggested that composition criteria for honeydew honey should be established in the food code in accordance with international standards. The principal component analysis reported in this study resulted in improved classification of the honeys from Korean beekeepers.

• Journal of the Korean Society of Clothing and Textiles
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• v.38 no.2
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• pp.201-215
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• 2014

This study explores the kinds and types of dyes used in the Joseon Dynasty. Some literature including "Sanga Yorok", "Gyuhap Chongseo", "Imwon Gyeonjeji" were reviewed for this purpose. The findings are as follows. The colors obtained from the dyes were divided into seven color series, such as red, yellow and orange, green, blue, purple, brown, gray and black. These are practical color categories differed from abstract five cardinal colors [五方色]. The diachronic dyes used during the Joseon Dynasty were safflower and Japanese alder bark for red, indigo leaves for blue, gromwell for purple, chestnut tree bark for brown. The representative dyes used in the late Joseon Dynasty were safflower and sappan wood for red, wild pear tree bark and Pentapetes phoenicea for yellow and orange, indigo leaves for blue, sappan wood and gromwell for purple, mulberry tree for brown, and Chinese ink for gray. Common dyes used both in Joseon and China were safflower and sappan wood for red, pagoda tree blossom for yellow, a combination of pagoda tree blossom (or Amur cork tree) and indigo leaves (or sediment) for green, indigo leavers or sediment for blue, sappan wood and hollyhock for purple, and a combination of indigo leaves and Galla Rhois for gray and black.