• 혜화의학회지
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• 제4권2호
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• pp.335-336
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• 1996

Artemisinin, a new antimalarial to treat patients infected with strains of Plasmodium jalciparum, derived from the plant Artemisia annua Linn, has immunopharmacologic actions such as enhence the PHA -induced lymphocyte transformation rate, increased the weight of spleen but reduced the weight of thymus, reduced phagocytic function of peritoneal macrophage, remarkably reduced the level of serum IgG and hemolysin fonning capacity (sentitized with SRBC), inhibited the activity of Ts cells of donor mice by supraoptimal immunuization(SOI), but enhenced activity of Ts cells of recipient mice by SOI. These results suggested that Ts cells may be the target cells of artemisinin. To the serum complement C3 level of plasmodium berghei-infeted mice, artemisinin (i. m,) could remarkly increase it. The artemisinin also obviously reduced the prostaglandin E(PGE) in the mouse hind paw swelling induced by carrageenin. Numerous studies have demonstrated that pharmacologic doses of PGE attenuate the development of immunocomplex nephritis. Some autologous immune mechanisms may be invoolved In the pathogensis of some types of glomurulonephritis. Glomerular abnormalities can be induced in animals by variety of immunological manipulations. The resulting disorder has many clinical and pathogical similarities to the disease in human. Our purpose was therefore to test the ability of the artemisinin to lessen the severity of rabbit IgG accelerated nephrotoxic serum glomerulonephritis in mice model. Mice which had treated with rabbit IgG and NTS, administrated with saline, showed Significant inceases of urinary protein, cholesterol level, and decrease of serum albumin in NS group. On the contrary, By i.g. adminstration of artemisinin at dose of 12.5, 25 and 50 mg/kg for 14 days after NTS injection, shown that artemisinin inhibited the nephritic changes in some parameters by means of urinary protein(p<0.05, p<0.01) and serum choleterol(p<0.05, p<0.01) and albumin (p<0.05, p<0.01), blood urea nitrogen (p<0.05, p<0.01), serum albumin(p<0.05, p<0.01); Cyclophosphamide(i.p. 10mg/kg for 14d) had almost same effect as the artemisinin had. Morphological studies shown that The picture of kidney from the mouse with NTS-nephritis accerated with rabbit IgG, treated with i.g. saline as the control, the mesangiocapillary were enlarged and proliferated; There were inflammatory cells infiltrating around the glomeruli; The ethelial cell were proliferated in the wall of Bowman's capsule. Histopatholological picture of kidney from the NTS-nephritis accerated with rabbit IgG mouse treated with i.p. 10mg/kg cyclophosphamide as the positive control. No siginicant histopathological evidence were found. Treaded with i.p. 12.5mg/kg artemisinine, the picture shown that mesangiocapillary were lightly proliferated; There were inflammatory cells infiltrating around the glomeruli; Treaded with i.p. 25mg/kg artemisinine, The picture shown that the mesangiocapillary were lightly proliferated; Treaded with i.p. 50mg/kg artemisinine, The picture shown that both the mesangiocapillary proliferated and the inflammatory cells infiltrating around the glomeruli are less than treated with saline, 12.5 and 25 mg/kg artemisinine. On the basis of these studies we conclude that the artemisinin can relieve pathological change caused by NTS-nephritis aacerated with rabbit IgG.

• The Korean Journal of Physiology
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• 제18권2호
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• pp.171-180
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• 1984

The death of cattle from acute bracken poisoning has been recognized for many years. Acute bracken poisoning is characterized by mucoidal nasal and anal hemorrhage, severe anorexia. pyrexia, gastric ulcer and myocardial damage. In 1958 Evans first suggested that clinical picture of bracken poisoning was very much similar to that of radiation injuries such as aplastic anemia, leucopenia, thrombocytopenia and increased capillary fragility. Bracken has been clearly demonstrated to contain a carcinogen as well as thiaminase. However, the nature of carcinogen in bracken has not definetely elucidated. Also it was warned by several workers that bracken could be a causative factor for stomach cancer in Korean and Japanese. It appears that little is known on the e(feet of bracken on the function of cardiovascular system. Therefore the present study was designed to explore effects of ethanol extract of raw and toiled bracken (RBEE:BBEE) on blood pressure in cats. Also studied was the mechanism underlying changed in blood pressure of cats by bracken. The result obtained were as follows; 1) Mean arterial blood pressure was invariably decreased following administration of either RBEE or BBEE. Tn general depressor responses to RBEE persisted longer than that to BBEE. Generally, depressor responses were proportional to debases of RBEE and BBEE administered. 2) After administration of 60 mg/kg RBEB and BBEE, blood Pressure decreased by $62.1{\pm}1.7mmHg$ and $68.0{\pm}3.0mmHg$, respectively. No change was observed between depressor responses to RBEE and that to BBEE. 3) Depressor responses to BBEE and RBEE were not affected by vagotomy, propranolol and regitine. 4) In atropinized animal depressor responses to BBEE and RBEE were reduced by 30-40% showing part of depressor response was resulted from cholinergic effect of bracken.

• 한국식품영양과학회지
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• 제19권1호
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• pp.53-60
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• 1990

농촌 주부의 식생활상태는 건강과 밀접한 관계를 가지므로 식생활환경과 식사내용 및 혈액 성상에 대한 내용을 분석 비교하였다. 대상자의 학력은 낮으며 가족수는 전국평균보다 많으며 약한 저체중상태가 60%나 되었다. 가정기기의 사용률은 중간 정도이나 농기계 보유율은 1.36대에 불과 하였으며 위생생활은 이루어진다고 볼 수 있으나 문화생활수준은 매우 낮았다. 혈압은 정상보다 낮으며 농부증은 4.72점으로서 요관찰대상점수였다. 식품군별 섭취량은 전반적으로 낮았으며 곡류와 채소가 주류이고 동물성식품은 빈약하였다. 영양소는 비타민C 및 아니아신을 제외하고 모두 권장량에 미치지 못하였으며 칼슘은 66.3%, 철분은 56.6%로서 특히 낮았다. 혈액성상은 혈청단백질은 정상으로 지질, 칼슘, 마그네슘 등은 정상범위는 벗어나지 않으나 낮은 수준이었다. 혈청철분과 헤모글로빈, 헤마토크릿은 낮은 수치가 많아 빈혈빈도가 높음을 보였으며 혈청 ferritin은 특히 낮았다. 각 요인의 상관관계를 보면, 곡류를 혈청마그네슘과 높은 상관을 보였고 혈청콜레스테롤은 곡류, 유지류, 채소류, 육류의 섭취와 상관을 보이고 체중과 신체충실지수와 밀접한 상관을 보였다. 빈혈과 관련하여 볼 때 헤모글로빈과 헤마토크릿 수치는 과일류, 생선류의 섭추와는 음의 상관을, 신체충실지수와는 양의 상관을 보였다.

• 대한수의사회지
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• 제18권11호
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• pp.48-53
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• 1982

본 실험은 닭의 근위점막 분말의 투여가 동물의 혈액학적 변화에 미치는 영향을 구명하기 위하여 근위점막(계내금)을 1일 사료섭취량의 $1\%$구(T.), $2\%$구$(T_2)$, $3\%$구$(T_3)$ 및 대조구(무처리구)에서 Babcock B-380의 성계 60수를 공시하여 8주간 Cage에서 사육한 후 혈액상을 조사한 결과는 다음과 같다. 1. 각 처리구별 적혈구치는 $T_3$구$(2.57{\pm}0.26\times10^6/mm^3)$, $T_2$구$(2.23{\pm}0.10\times10^6/mm^3)$, $T_1$구$(2.38{\pm}0.40\times10^6/mm^3)$ 및 C구$(2.17{\pm}0.10\times10^6/mm^3)$의 순이었고 그 평균치는 $2.34{\pm}0.21\times10^6/mm^3$로서, 대체로 처리구(첨가구)가 대조구(무처리구)보다 유의적으로 높은 경향이었다. 2. 각 처리구별 Hemoglobin함량은 $T_3$구$(9.13{\pm}0.14gm/100ml)$, $T_2$구$(7.78{\pm}0.40gm/100ml)$ 및 $T_1$구$(7.44{\pm}0.48gm/100ml)$의 순이었고 그 평균치는 $8.06{\pm}0.28gm/100ml$ 로서, 처리구와 대조구간에 유의성은 확인되지 않았다. (p<0.01). 3. 각 처리구별 백혈구치는 C구$(1.81{\pm}0.63\times10^4/mm^3)$, $T_1$구$(1.69{\pm}0.12\times10^4/mm^3) $, $T_2$구$(1.61{\pm}0.16\times10^4/mm^3)$ 및 $T_3$구$(1.56{\pm}0.20_10^4/mm^3)$의 순이었고 그 평균치는 $1.67{\pm}0.23\times10^4/mm^3$으로서, 첨가량이 많아질수록 낮아지는 경향이었다. 4. 밸혈구백분비는 Lymphocytes $60.98{\pm}1.76\%$, Heterophils $22.75{\pm}2.27\%$, Eosinophils $3.17{\pm}0.95\%$, Basophils $2.17{\pm}0.85\%$ 및 Monocytes $10.84{\pm}2.02\%$였다. 5. 적혈구의 크기는 $12.01{\pm}0.74\mu\times6.82{\pm}0.10\mu\times3.57{\pm}0.08\mu$(장경$\times$단경$\times$두께)였다.

• 한국임상수의학회지
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• 제8권1호
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• pp.109-117
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• 1991

This experiment was carrid out to investigate whether Aloe arborescens had a radioprotective effect in cobalt-60 gamma irradiated(600 and 700ra4s) mice. The survival ratio of mice for 30 days and blood pictures were investigated. 1. The survival ratios of 600ra4s gamma Irradiated(dose rate 50rads/min.) in pre-irradiation aloe administered group(A), post-irradiation aloe administered group(B) and control group were 73.3%, 72.3% and 63.3%, and those of 700ra4s groups were 63.3%, 60.0% and 51.7%, respectively. 2. The total leucocyte counts of aloe administered groups were less decreased and more rapidly recovery to normal level than those of control group. 3. The lymphocyte counts were rapidly decreased on the 1st day after irradiation in all experimental groups than those of control group. 4. The recovery effects of total leucocyte counts of pre-irradiation aloe administered groups were superior to those of post-irradiation aloe groups and control group.

• 한국가축번식학회지
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• 제18권3호
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• pp.157-165
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• 1994

This experiment was examined the effect of splenectomy on the hematology in pregnant wistar rat. Only animals that had been shown regular 4-day estrous cycles for more than two cycles were used. The day after mating with the same male animal ws designated Day 0 of pregnancy. Spleen was removed from Day 0(early), 6(middle) and 13(late) of pregnant rat, respectively. Blood sample was collected at Day 1, 7, 14 and 21 of the pregnancy. 1. RBC was increased significantly(P<0.05) to the progress of pregnancy in control rat. The late splenectomized rats were decreased significantly(P<0.05) at Day 21 of pregnancy than control rats. 2. Hb was increased significantly (P<0.05) at 21th day of pregnancy in late splenectomized groups than others group. 3. In the late splenectomized rats, Ht was decreased significant (P<0.05) due to the progress of pregnancy and decreased significantly (P<0.05) at Day 21 of pregnancy in all splenectomized groups. 4. WBC was increased significantly (P<0.05) at Day 1 of pregnancy in splenectomized groups compared with control. 5. In differential leukocyte rate, the Basophils and Monocytes was not significantly changed. Neutrophils was increased significantly(P<0.05) at Day 14 and 21 than Day 1 and 7 of pregnancy in control. Lymphocytes was decreased significantly(P<0.05) in control due to progress of pregnancy. Neutrophils was increased and Lymphocytes was decreased significantly(P<0.05) in splenectomized groups compared with control.

• 대한수의학회지
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• 제22권1호
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• pp.63-66
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• 1982

Authors encountered a case of bovine viral leukosis in a young cattle (10 months old) with the following findings; Main clinical symptoms observed were emaciation, digestive disorder, and marked enlargement of superficial lymph nodes. Biopsied specimen of the lymph nodes revealed characteristic histological picture of bovine leukosis. A severe degree of leukocytosis (108,000/cmm) accompanied by marked lympocytosis (96%=103,680/cmm) was detected from the hematological examination of peripheral blood. Most of the lympocytes(94%=101,520) were large-sized, immature, and abnormal in their morphology. In addition, a pronounced anemia (1.78 million/cmm) without regenerative evidence of erythrocyte was noted. The serum of the patient showed positive reaction against gp antigen of bovine leukosis virus in agar-gel diffusion test.

• Asian-Australasian Journal of Animal Sciences
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• 제12권5호
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• pp.728-734
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• 1999

Thirty new born crossbred (Bos taurus${\times}$Bos indicus) calves, divided randomly in a $3{\times}2$ factorial design, were fed calf starters containing one of three protein sources i.e., groundnut cake (GN), cottonseed meal (CS) and meat and bone meal (MB) along with either raw (M) or gelatinized maize (MG) for 90d. Milk was fed upto 56d of age. Green oats and respective calf starters were offered from 14d of age onwards ad lib. Clinical profile of serum suggested significantly (p<0.05) higher albumin and lower alanine aminotransferase activity due to CS feeding. Alklaine phosphatase activity varied significantly (p<0.05) among dietary treatments showing interaction between protein and starch sources. Inclusion of gelatinized maize resulted in significantly higher concentration of serum globulin (p<0.05) and alkaline phosphatase activity (p<0.01). reduced (p<0.05) ruminal pH was accompanied by a significant decrease (p<0.01) in $NH_3-N$ concentration in the strained rumen liquor (SRL) of MG fed calves. Ruminal amylase activity was lower (p<0.05) on MG diets. Alanine aminotransferase activity in the rumen exhibited a significant (p<0.01) interaction between protein and starch sources. While feeding of CS significantly (p<0.01) reduced alanine aminotransferase activity, inclusion of thermally processed maize reduced (p<0.01) both aspartate and alanine aminotransferase activities in the rumen. The overall blood picture was similar among treatments, whereas rumen metabolites especially enzyme activities, seems to be altered with source of degradable protein an starch.

• Journal of Periodontal and Implant Science
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• 제52권1호
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• pp.65-76
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• 2022

Purpose: The present study measured changes in arteriolar and venular capillary flow and structure in the gingival tissues during the development of plaque-induced gingival inflammation by combining dynamic optical coherence tomography (OCT), laser perfusion, and capillaroscopic video imaging. Methods: Gingival inflammation was induced in 21 healthy volunteers over a 3-week period. Gingival blood flow and capillary morphology were measured by dynamic OCT, laser perfusion imaging, and capillaroscopy, including a baseline assessment of capillary glycocalyx thickness. Venular capillary flow was estimated by analysis of the perfusion images and mean blood velocity/acceleration in the capillaroscopic images. Readings were recorded at baseline and weekly over the 3 weeks of plaque accumulation and 2 weeks after brushing was resumed. Results: Perfusion imaging demonstrated a significant reduction of gingival blood flow after 1 and 2 weeks of plaque accumulation (P<0.05), but by 3 weeks of plaque accumulation there was a more mixed picture, with reduced flow in some participants and increased flow in others. Participants with reduced flux at 3 weeks also demonstrated venular-type flow as determined by perfusion images and evidence of the development of venular capillaries as assessed by the velocity/acceleration ratio in capillaroscopic images. After brushing resumed, these venular capillaries were broken down and replaced by arteriolar capillaries. Conclusions: After 3 weeks of plaque accumulation, there was wide variation in microvascular reactions between the participants. Reduced capillary flow was associated with the development of venular capillaries in some individuals. This is noteworthy, as an early increase in venous capillaries is a key vascular feature of cardiovascular disease, psoriasis, Sjögren syndrome, and rheumatoid arthritis-diseases with a significant association with the development of severe gingival inflammation, which leads to periodontitis. Future investigations of microvascular changes in gingival inflammation might benefit from accurate capillary flow velocity measurements to assess the development of venular capillaries.

• Journal of the Korean Data and Information Science Society
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• 제22권2호
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• pp.287-296
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• 2011

36명의 여대생을 대상으로 체 지방 감소효과에 대한 실험을 실시하였다. 이 실험에서 처리는 매일 섭취하는 식사종류 및 양에 대한 식사일지 작성과 카메라 폰으로 찍어 실험관리자에게 전송하여 매주상담을 받는 것이다. 실험관리자는 체 지방 및 관련된 자료를 일주일마다 측정하여 8주간의 반복측정자료를 얻었다. 이 실험자료를 이용하여 혼합모형의 일종인 변량계수모형을 이용하여 추정 및 유의성 검정을 실시한 결과, 유의한 고정인자들은 처리 전체지방 값, 비만지수, 확장기 혈압, 총 콜레스테롤 및 시간이다. 처리 후 시간에 따른 체 지방 감소는 2차 함수의 관계가 성립된다. 변량인자인 개체효과와 개체와 시간과의 교호작용에서 1차 함수의 관계가 존재한다. 처리 후 시간이 지남에 따라 체 지방 량은 점점 감소하였으며, 실험실시 8주 후에는 평균 2.1kg 감소한 효과가 있음을 보여주었다.