• Title/Summary/Keyword: Block Replication

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Effects of Soil Organic Amendment as Plant Growing Media Component for Restoration of Planting Ground (식재기반 복원을 위한 유기질계 토양개량재의 효용성)

  • Ju, Jin-Hee;In, Da-Young;Kim, Won-Tae;Yoon, Young-Han;Choi, Eun-Young
    • Journal of Environmental Science International
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    • v.24 no.11
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    • pp.1363-1370
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    • 2015
  • This study was aimed to determine effects of soil organic amendment as plant growing media component on restoration of planting ground. The changes of soil physical and chemical properties and germination and growth of kentucky bluegrass (Poa pratensis L.) were investigated. For treatments, soil was excavated at depth of 0-50 cm (referred as $S_1$) and at depth of 50-100 cm (referred as $S_2$). Then the half amount of $S_1$ soil was mixed with the soil organic amendment (coir dust 40% (v/v), bottom ash 25%, leaf mold 25%, vermiculite 5%, carbonized rice hull 5%) at a rate of 6% (v/v) (referred as $S_1CC$) and also the half amount of $S_2$ soil was mixed with the soil organic amendment at a rate of 6% (v/v) (referred as $S_2CC$) on pot in a 16 cm diameter and 14 cm height. The experiment was replicated 3 times with 3 pots per replication in randomized block design, and 100 seeds were planted per pot. In results, there was no significant difference in soil pH among the treatments with a slight decrease in soil hydraulic conductivity. However, in the $S_1CC$ treatment, positive increases in soil chemical properties, including electrical conductivity, organic matter, phosphoric acid, total nitrogen, exchangeable cation, and cation exchange capacity. Also, the germination rate, plant height, and number of leaves were higher in the $S_1CC$ treatment than those in other treatments. These results suggest that the addition of organic amendment to the soil at depth of 0-50 cm might be proper for restoring planting ground.

Effects of 8 weeks administration of Korean Panax ginseng extract on the mood and cognitive performance of healthy individuals

  • L., Reay J.;B., Scholey A.;O., Kennedy D.
    • Journal of Ginseng Research
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    • v.31 no.1
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    • pp.34-43
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    • 2007
  • Background: Previous research has suggested that single doses of a standardised Panax ginseng extract can decrease fasted blood-glucose levels and modulate cognitive performance in healthy young volunteers. The latter has generally been seen in terms of improved secondary memory performance. However, both the cognitive effects of chronic administration of ginseng and the potential modulation of working memory have received comparatively little research attention. Aims: The current double-blind, placebo-controlled, balanced cross-over study investigated the effects of 8-weeks administration of Korean ginseng extract (200 mg) on cognitive performance, gluco-regulatory parameters and ratings of subjective mood and 'quality of life'. Methods: 'Eighteen healthy young participants were assessed pre-dose and 3 hours post-dose on the mornings of Day 1, Day 29 and Day 57 of 8 week treatment regimens of both placebo and ginseng. A four-week placebo wash-out separated the treatment phases. Each assessment included the Cognitive Drug Research battery, computerised working memory tasks, and Bond-Lader mood scales. The WHO Quality of Life scale (WHOQOL-BREF) was completed once per visit. Gluco-regulatory parameters were assessed with assays of blood glucose, insulin and HbA1c. Results: Data from the 16 participants that completed the study showed that there were no significant, acute treatment related differences on Day 1 of treatment, or in gluco-regulatory parameters throughout the study. However, time related performance improvements were evident following chronic administration of ginseng on the '3-Back' and 'Corsi-block' computerised working memory tasks. Ginseng was also associated with an improved score on the 'social relations' subscale of the WHOQOL-100, and a significant shift on the 'calm' factor of the Bond-Lader mood scales (from calm/relaxed towards excited/tense). Conclusion: The results of the current study suggest that Korean ginseng extract can modulate working memory performance and subjective ratings of 'quality of life' and mood. Replication with a larger sample size may further elucidate the actions of this product.

Selective Interaction Between Chloroplast β-ATPase and TGB1L88 Retards Severe Symptoms Caused by Alternanthera mosaic virus Infection

  • Seo, Eun-Young;Nam, Jiryun;Kim, Hyun-Seung;Park, Young-Hwan;Hong, Seok Myeong;Lakshman, Dilip;Bae, Hanhong;Hammond, John;Lim, Hyoun-Sub
    • The Plant Pathology Journal
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    • v.30 no.1
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    • pp.58-67
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    • 2014
  • The multifunctional triple gene block protein 1 (TGB1) of the Potexvirus Alternanthera mosaic virus (AltMV) has been reported to have silencing suppressor, cell-to-cell movement, and helicase functions. Yeast two hybrid screening using an Arabidopsis thaliana cDNA library with TGB1 as bait, and co-purification with TGB1 inclusion bodies identified several host proteins which interact with AltMV TGB1. Host protein interactions with TGB1 were confirmed by biomolecular fluorescence complementation, which showed positive TGB1 interaction with mitochondrial ATP synthase delta' chain subunit (ATP synthase delta'), light harvesting chlorophyll-protein complex I subunit A4 (LHCA4), chlorophyll a/b binding protein 1 (LHB1B2), chloroplast-localized IscA-like protein (ATCPISCA), and chloroplast ${\beta}$-ATPase. However, chloroplast ${\beta}$-ATPase interacts only with $TGB1_{L88}$, and not with weak silencing suppressor $TGB1_{L88}$. This selective interaction indicates that chloroplast ${\beta}$-ATPase is not required for AltMV movement and replication; however, TRV silencing of chloroplast ${\beta}$-ATPase in Nicotiana benthamiana induced severe tissue necrosis when plants were infected by AltMV $TGB1_{L88}$ but not AltMV $TGB1_{L88}$, suggesting that ${\beta}$-ATPase selectively responded to $TGB1_{L88}$ to induce defense responses.

Changes in growth performance, nutrient digestibility, immune blood profiles, fecal microbial and fecal gas emission of growing pigs in response to zinc aspartic acid chelate

  • Jiao, Yang;Li, Xinran;Kim, In Ho
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.4
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    • pp.597-604
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    • 2020
  • Objective: This study was conducted to investigate the effect of zinc aspartic acid chelate (Zn-ASP) on growth performance, nutrient digestibility, blood profiles, fecal microbial and fecal gas emission in growing pigs. Methods: A total of 160 crossbred ([Landrace×Yorkshire]×Duroc) growing pigs with an initial body weight (BW) of 25.56±2.22 kg were used in a 6-wk trial. Pigs were randomly allocated into 1 of 4 treatments according to their sex and BW (8 replicates with 2 gilts and 3 barrows per replication pen). Treatments were as follows: i) CON, basal diet, ii) TRT1, CON+0.1% Zn-ASP, iii) TRT2, CON+0.2% Zn-ASP, and iv) TRT3, CON+0.3% Zn-ASP. Pens were assigned in a randomized complete block design to compensate for known position effects in the experimental facility. Results: In the current study, BW, average daily gain, and gain:feed ratio showed significant improvement as dietary Zn-ASP increased (p<0.05) in growing pigs. Apparent total tract digestibility (ATTD) of dry matter was increased linearly (p<0.05) in pigs fed with Zn-ASP diets. A linear effect (p<0.05) was detected for the Zn concentration in blood with the increasing levels of Zn-ASP supplementation. Lactic acid bacteria and coliform bacteria were affected linearly (p<0.05) in pigs fed with Zn-ASP diets. However, no significant differences were observed in the ATTD of nitrogen, energy and Zn. And dietary Zn-ASP supplementation did not affect fecal ammonia, hydrogen sulfide and total mercaptans emissions in growing pigs. Conclusion: In conclusion, dietary supplementation with Zn-ASP of diet exerted beneficial effects on the growth performance, nutrient digestibility, blood profiles and fecal microbes in growing pigs.

Effects of Herbicide Application Method on Abutilon avicennar Control and Corn Yield (제초제 살포방법이 어저귀 방제 및 옥수수의 생산성에 미치는 영향)

  • Kang, W.S.;Kim, J.G.;Chung, E.S.;Seo, S.;Yang, J.S.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.18 no.2
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    • pp.107-112
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    • 1998
  • This experiment was carried out to investigate effects of herbicide application method on Abutilon avicennae control and corn yield at the forage experimental field, grassland and forage crop division, National Livestock Research Institute, RDA, Suwon from 1996 to 1997. The experiment was arranged in a randomized block design with three replication. The herbicide application consisted of control, 2-3 leaf stage(Dicamba), 5-6 leaf stage (Dicamba) and soil treatment(Pendimetha1in). The hybrid of corn used in this experiment was P 3352. The results obtained are summarized as follow; 1. The plant and ear height was the lowest at control. Tasseling and silking date were delayed 3-4 day at control. The length and weight of ear were the highest at Dicamba treatment in 2-3 leaf stage. 2. The herbicide injury of corn was detected slightly after 10-20 day but the injury was recovered soon. The weed control efficiency was 96.7 and 81.8% at Dicamba treatment in 2-3 and 5-6 leaf stage, respectively. 3. The fresh and dry matter yield of Dicamba treatment in 2-3 leaf stage was higher than that of control by 36%, but no significant difference was found among herbicide application method. 4. Herbicide application method had little effect on the ADF, NDF and CP contents, but the CP and IVDMD of ear were higher than that of stover. The results of this experiment indicate that the application of Dicarnba in 2-6 leaf stage of corn will remove almost all of A butilon avicennae and increase DM yield of corn.

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Potential of Hanjeli (Coix lacryma-jobi) essential oil in preventing SARS-CoV-2 infection via blocking the Angiotensin Converting Enzyme 2 (ACE2) receptor

  • Diningrat, Diky Setya;Sari, Ayu Nirmala;Harahap, Novita Sari;Kusdianti, Kusdianti
    • Journal of Plant Biotechnology
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    • v.48 no.4
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    • pp.289-303
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    • 2021
  • Covid-19 is an ongoing pandemic as we speak in 2022. This infectious disease is caused by the SARS-CoV-2 virus, which infects cells by binding to the angiotensin-converting enzyme 2 (ACE2) receptor on the cell surface. Thus, strategies that inhibit the binding of SARS-CoV-2 to the ACE2 receptor can stop this contagion. Hanjeli (Coix lacryma-jobi) essential oil contains many bioactive compounds, including dodecanoic acid; tetradecanoic acid; 7-Amino-8-imino-2-(2-imino-2H-chromen-3-yl); and 1,5,7,10-tetraaza-phen-9-one. These compounds suppress viral replication and may prevent Covid-19. Accordingly, this study assessed whether, these four limonoid compounds can block the ACE2 receptor. To this end, their physicochemical properties were predicted using Lipinski's "rule of five" on the SwissADME website, and their toxicity was assessed using the online tools ProTox and pkCSM. Additionally, their interactions with the ACE2 receptor were predicted via molecular docking using Autodock Vina. All the four compounds satisfied the "rule of five" and tetradecanoic acid was predicted to have a higher affinity than the comparison compound remdesivir and the original ligand of ACE2. Molecular docking results suggested that the compounds from hanjeli essential oil interact with the active site of the ACE2 receptor similarly as the original ligand and remdesivir. In conclusion, hanjeli essential oil contains compounds predicted hinder the interaction of SARS-CoV-2 with the ACE2 receptor. Accordingly, our data may facilitate the development of a phytomedical strategy against SARS-CoV-2 infection.

Effect of dietary phytase supplementation with different calcium/phosphorus ratio and net energy reduction on growth performance and nutrient digestibility in finishing pigs

  • Yanjiao Li;Qianqian Zhang;In Ho Kim
    • Korean Journal of Agricultural Science
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    • v.49 no.4
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    • pp.639-646
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    • 2022
  • The present experiment was conducted to assess the effect of Buttiauxella-derived phytase in finishing pigs fed corn/soybean meal diets with an increase in the calcium (Ca)/total phosphorus (P) (Ca/tP) ratio and a reduction in net energy on the growth performance and nutrient digestibility on the finishing pigs. A total of 90 crossbred ([Yorkshire × Landrace] × Duroc) finishing pigs with an average initial body weight (BW) of 56.94 ± 2.43 kg were used for an 11-week feeding trial. The pigs were randomly allotted to one of three dietary treatments (six replication/treatment and five pigs/pen) in a randomized complete block design according to their BW and gender. Dietary treatments consisted of supplementation of phytase (0.05, 0.07, and 0.1% in the control, Trt1 and Trt 2 diets, respectively) maintaining the Ca/tP ratio (1.67 : 1, 1.84 : 1, and 2.19 : 1 in control, Trt1 and Trt 2 diets, respectively) and reducing the net energy by 1% in Trt1 and Trt2 diets compared with the control diet. The results showed that dietary supplementation with phytase in the energy-reduced diet had a similar (p > 0.05) effect on the BW, average daily gain, average daily feed intake, and gain/feed ratio of the finishing pigs. Also, there were no effects (p > 0.05) of treatment matrixes on the nutrient digestibility of dry matter, nitrogen calcium, phosphorus, and gross energy. In conclusion, the increase of phytase and Ca/tP ratio and the reduction of net energy in the corn/soybean meal diet resulted in comparable growth performance and nutrient digestibility of finishing pigs relative to pigs fed the control diet.

Development of CPLD technology mapping control algorithm for Sequential Circuit under Time Constraint (시간제약 조건하에서 순차 회로를 위한 CPLD 기술 매핑 제어 알고리즘 개발)

  • Youn, Chung-Mo;Kim, Jae-Jin
    • Journal of the Korean Institute of Telematics and Electronics T
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    • v.36T no.4
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    • pp.71-81
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    • 1999
  • We propose a new CPLD(Complexity Programmable Logic Device) technology mapping algorithm improving run-time under time constraint. In our technology mapping algorithm, a given logic equation is constructed as the DAG(Directed Acyclic Graph) type, then the DAG is reconstructed by replicating the node that outdegree is more than or equal to 2. As a result, it makes delay time and the number of CLBs, run-time to be minimized. Also, after the number of multi-level is defined and cost of each nodes is calculated, the graph is partitioned in order to fit to k that is the number of OR term within CLB. The partitioned nodes are merged through collapsing and bin packing is performed in order to fit to the number of OR term within CLB(Configurable Logic Block). In the results of experiments to MCNC circuits for logic synthesis benchmark, we can shows that proposed technology mapping algorithm reduces run-time and the number of CLBs much more than the TEMPLA.

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Dietary Requirement of True Digestible Phosphorus and Total Calcium for Growing Pigs

  • Ruan, Z.;Zhang, Y.-G.;Yin, Y.-L.;Li, T.-J.;Huang, R.-L.;Kim, S.W.;Wu, G.Y.;Deng, Z.Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.8
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    • pp.1236-1242
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    • 2007
  • Sixty healthy growing pigs ($Duroc{\times}Landrace{\times}Yorkshire$ with an average BW of 21.4 kg) were used to determine the true digestible phosphorus (TDP) requirement of growing pigs on the basis of growth performance and serum biochemical indices. Pigs were assigned randomly to one of five dietary treatments (12 pigs/diet), representing five levels of TDP (0.16%, 0.20%, 0.23%, 0.26% and 0.39%). There were three replications per treatment, with four pigs (2 barrows and 2 gilts) in each replication (2 pigs/pen) A randomized-block design was used, with pen as the experimental unit. Experimental diets were formulated to provide the 5 TDP levels with a total calcium (Ca) to TDP ratio of 2:1, and offered to pigs at 5% BW for 28 d. The total Ca contents of the five diets were 0.33, 0.38, 0.45, 0.51 and 0.79%, respectively. During the 28-d experimental period, the ADG of pigs was affected by dietary TDP levels as described by Equation 1: y = $-809,532x^4+788,079x^3-276,250x^2+42,114x-1$,759; ($R^2$ = 0.99; p<0.01; y = ADG, g/d; x = dietary TDP, %). The feed:gain ratio for pigs was affected by dietary TDP levels as described by Equation 2: y = $3,651.1x^4-3,480.4x^3+1,183.8x^2-172.5x+10.9$ ($R^2$ = 0.99; p<0.01; y = feed:gain ratio; x = dietary TDP, %). Total P concentrations in serum were affected by dietary TDP levels as described by Equation 3: y = $-3,311.7x^4+3,342.7x^3-1,224.6x^2+195.6x-8.7$ ($R^2$ = 0.99; p<0.01; y = total serum P concentration and x = dietary TDP, %). The highest ADG (782 g/d), the lowest feed:gain ratio (1.07), and the highest total serum P concentration (3.1 mmol/L) were obtained when dietary TDP level was 0.34%. Collectively, these results indicate that the optimal TDP requirement of growing pigs is 0.34% of the diet (e.g., 5.1 g/day for a 30-kg pig that consumed 1.5 kg feed daily) at a total Ca to TDP ratio of 2:1.

Protected Organic Acid Blends as an Alternative to Antibiotics in Finishing Pigs

  • Upadhaya, S.D.;Lee, K.Y.;Kim, In Ho
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.11
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    • pp.1600-1607
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    • 2014
  • A total of 120 finishing pigs ([Yorkshire${\times}$Landrace]${\times}$Duroc) with an average body weight (BW) of $49.72{\pm}1.72kg$ were used in 12-wk trial to evaluate the effects of protected organic acids on growth performance, nutrient digestibility, fecal micro flora, meat quality and fecal gas emission. Pigs were randomly allotted to one of three dietary treatments (10 replication pens with 4 pigs per pen) in a randomly complete block design based on their initial BW. Each dietary treatment consisted of: Control (CON/basal diet), OA1 (basal diet+0.1% organic acids) and OA2 (basal diet+0.2% organic acids). Dietary treatment with protected organic acid blends linearly improved (p<0.001) average daily gain during 0 to 6 week, 6 to 12 week as well as overall with the increase in their inclusion level in the diet. The dry matter, N, and energy digestibility was higher (linear effect, p<0.001) with the increase in the dose of protected organic acid blends during 12 week. During week 6, a decrease (linear effect, p = 0.01) in fecal ammonia contents was observed with the increase in the level of protected organic acid blends on d 3 and d 5 of fermentation. Moreover, acetic acid emission decreased linearly (p = 0.02) on d7 of fermentation with the increase in the level of protected organic acid blends. During 12 weeks, linear decrease (p<0.001) in fecal ammonia on d 3 and d 5 and acetic acid content on d 5 of fermentation was observed with the increase in the level of protected organic acid blends. Supplementation of protected organic acid blends linearly increased the longissimus muscle area with the increasing concentration of organic acids. Moreover, color of meat increased (linear effect, quadratic effect, p<0.001, p<0.002 respectively) and firmness of meat showed quadratic effect (p = 0.003) with the inclusion of increasing level of protected organic acid in the diet. During the 6 week, increment in the level of protected organic acid blends decreased (linear effect, p = 0.01) Escherichia coli (E. coli) counts and increased (linear effect, p = 0.004) Lactobacillus counts. During 12-wk of experimental trial, feces from pigs fed diet supplemented with organic acid blends showed linear reduction (p<0.001) of E. coli counts and the tendency of linear increase (p = 0.06) in Lactobacillus count with the increase in the level of organic acid blends. In conclusion, 0.2% protected organic acids blends positively affected growth performance, nutrient digestibility, fecal gas emission and meat quality in finishing pigs without any adverse effects on blood parameters.