• Journal of Marine Bioscience and Biotechnology
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• v.10 no.1
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• pp.1-8
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• 2018

The objective of this study was to purify and characterize the ${\beta}-secretase$ inhibitor from enzymatic hydrolysates of blackfin flounder muscle, for development of a novel anti-dementia agent that may be used in the drug or functional food industries. ${\beta}-secretase$ inhibitory peptide was purified from various enzymatic hydrolysates of blackfin flounder muscle. Among six enzymatic hydrolysates, the Alcalase hydrolysate revealed highest ${\beta}-secretase$ inhibitory activity. Consecutive purification of the blackfin flounder muscle hydrolysate using Sephadex G-25 column chromatography and octadecylsilane C18 reversed phase HPLC techniques were used to isolate a potent ${\beta}-secretase$ inhibitory peptide composed of 5 amino acids, Leu-Thr-Gln-Asp-Trp (MW: 526.7 Da). The $IC_{50}$ value of purified ${\beta}-secretase$ inhibitory peptide was $126.93{\mu}M$. Results of this study suggest that peptides derived from blackfin flounder muscle may be beneficial as anti-dementia compounds in functional foods or as pharmaceuticals.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.56 no.4
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• pp.347-360
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• 2020

In this study, the Bayesian state-space model was used for the stock assessment of the Blackfin flounder. In addition, effective measures for the resource management were presentedwith the analysis on the effectiveness of fisheries management plans. According to the result of the analysis using the Bayesian state-space model, the main biometric value of Blackfin flounder was analyzed as 1,985 tons for maximum sustainable yield (MSY), 23,930 tons for carrying capacity (K), 0.000007765 for catchability coefficient (q) and 0.31 for intrinsic growth (r). Also the evaluation on the biological effect of TAC was done. The result showed that the Blackfin flounder biomass will be kept at 14,637 tons 20 years later given the present TAC volume of 1,761 tons. If the Blackfin flounder TAC volume is set to 1,600 tons, the amount of biomass will increase to 16,252 tons in the future. Lastly, the biological effectiveness of the policy to reduce fishing effort was assessed. The result showed that the Blackfin flounder biomass will be maintained at 13,776 tons if the current fishing efforts (currently hp) level is set and maintained. If the fishing effort is reduced by 20%, it will increase to 17,091 tons in the future. The analysis on the economic effect of TAC showed that NPV will be the lowest at 1,486,410 won in 2038, 20 years after the establishment of 2,500 tons of TAC volume. If the TAC volume is set at 2,000 tons, NPV was estimated to be the highest at 2,206,522,000 won. In addition, the analysis on the economic effect of the policy to reduce the amount of fishing effort found that NPV will be 2,235,592,000 won in 2038, 20 years after maintaining the current level of fishing effort. If the fishing effort is increased by 10%, NPV will be the highest at 2,257,575 won even thoughthe amount of biomass will be reduced.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.48 no.4
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• pp.483-488
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• 2015

We studied oocyte steroidogenesis in the blackfin flounder Glyptocephalus stelleri as a region-specific species, in the East Sea of Korea during the spawning season. Maturing oocytes (0.76, 0.82, 0.88, and 0.91 mm in oocyte diameter) were incubated in vitro in the presence of [$^3H$] $17{\alpha}$-hydroxyprogesterone ($[^3H]17{\alpha}$-OHP) as a precursor. Steroid metabolites were extracted from the incubated medium and oocytes, and the extracts were separated and identified by thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC) and gas chromatographymass spectrometry (GC/MS). The major metabolites produced from $[^3H]17{\alpha}$-OHP were androgens [androstenedione (A4) and testosterone (T)] and estrogens [$17{\beta}$-estradiol (E2) and estrone (E1)] and progestins [$17{\alpha},20{\alpha}$-dihydroxy-4-pregen-3-one ($17{\alpha}20{\alpha}P$) and $17{\alpha}20{\beta}$-dihydroxy-4-pregnen-3-one ($17{\alpha}20{\beta}P$)] in maturing oocytes. The metabolic rate of $17{\alpha}20{\beta}$ was elevated (29.04%) in oocytes measuring 0.88 mm (nucleus migration stage following the induction of germinal vesicle breakdown), but was very low in oocytes measuring 0.76, 0.82, and 0.91 mm (0.42, 0.67, and 2.62%, respectively). From these results, we suggest that $17{\alpha}20{\beta}P$ acts as a maturation-inducing steroid in the blackfin flounder.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.5
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• pp.598-606
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• 2013

The blackfin flounder Glyptocephalus stelleri is a commercially important species in the East Sea of Korea, but its catches and biomass have decreased gradually in recent years. This study estimated the optimal catch (acceptable biological catch, ABC) for the effective management of this species by estimating population ecology parameters and the stock biomass of blackfin flounder in the East Sea of Korea. The estimated instantaneous coefficient of total mortality (Z) of blackfin flounder was 1.0542/year, the survival rate (S) was 0.3485, and the instantaneous coefficient of natural mortality (M) was 0.3637/year. From the values of S and M, the instantaneous coefficient of fishing mortality (F) was calculated to be 0.6905/year. The age at first capture was 1.304 years, and the total length was 11.5 cm at that time. On the basis of these parameters, the annual biomass was estimated by a biomass-based cohort analysis using annual catch data in weight by year for 1991-2012 in the East Sea of Korea. The annual biomass peaked in 1997 at about 12,800 mt and then subsequently declined continuously to a level of 10,500 mt in 2004 and to 9,800 mt in 2011 and 2012. The maximum sustainable yield and $F_{0.1}$ were estimated as 3,547 mt and 0.3595/year, respectively. Using these estimations, the ABC was estimated to be 3,571 mt in tier 5, 3,397 mt in tier 4, and 2,622 mt in tier 3.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.5
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• pp.654-658
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• 2014

We report the first identification of a spawning ground of the blackfin flounder Glyptocephlaus stelleri near the Wangdol-cho sea mountains, located in the southern East Sea. Eggs and larval fish of G. stelleri were collected during April and June, 2014, when an abundance of eggs was found in the southern area of Wangdol-cho. Our findings suggest that G. stelleri prefers to spawn in the vicinity of the off-shore sea mountains, where the temperature is between 10 and $12^{\circ}C$ and the water depth is 100 m, rather than inshore.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.52 no.4
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• pp.394-402
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• 2016

In order to manage and rebuild fishery resources, the fishing effort should be controlled effectively. Especially in the setting up of the proper level of fishing efforts, economic standards as well as biological standards must be carefully considered to promote the sustainable and economically viable development of fisheries. This study aimed to analyze the optimal economic fishing effort ($E_{MEY}$) as the most economically efficient one for the Eastern Sea Danish seine fisheries. The results showed that the optimal economic fishing effort ($E_{MEY}$) of Eastern Sea Danish seine fisheries for blackfin flounder should be reduced by about 27%. That is, reducing fishing efforts up to the level of $E_{MEY}$ could lead to the reduction of fishing costs, thereby resulting in the increased fishing profits.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.50 no.1
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• pp.1-11
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• 2014

To compare the catches made using gillnets, trammel nets, and gillnets with supporting lines, several experiments were conducted with commercial vessels near Uljin and Pohang in Eastern Korea between July 2010 and May 2011. Two sets of 13 different nets were used, including 5 panels of gillnets and trammel nets each with stretched mesh sizes of 7.6, 9.1, 10.6, 12.1, and 13.6 cm and 3 panels of gillnets with a mesh size of 9.1 cm with supporting lines with different line spacing. The outer (stretched) mesh size of the trammel nets measured 51.5 cm. The target fishes of the fishing nets were various types of flounders. The catch rate of flounders was 50.7% of the total catch in weight. The total catch for all nets was 443.8 kg. The predominant species was pointhead flounder (Cleisthenes pinetorum). The total catch by trammel nets was 1.4 times that of the comparable gillnets. But more pointhead flounder were caught by gillnets than by trammel nets, though there was no significant difference. Fishermen catching the pointhead flounder in Korea said that there was no need to use trammel net to catch it; this was an unexpected finding compared to the findings of other flounder fisheries. The amounts of roughscale sole, brown sole, and blackfin flounder caught by trammel nets were greater than those caught by gillnets. The mean lengths (standard deviation) of blackfin flounder, pointhead flounder, brown sole, and roughscale sole were 21.0 (4.57), 22.9 (3.40), 24.7 (4.90), and 28.3 (5.43) cm, respectively; there were significant differences in mean length (p < 0.00001). Therefore, in order to catch flounder efficiently, the fishing nets and mesh size should be chosen according to the target species. One advantage of using supporting lines is that it prevents breakage by strengthening the material especially when utilized on a rough bottom. Catch by using gillnet with supporting lines was not greater than that by using trammel net for the conservation of fisheries resources.

• Fisheries and Aquatic Sciences
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• v.15 no.2
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• pp.169-176
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• 2012

Age and growth of Glyptocephalus stelleri in the East Sea of Korea were determined, from monthly samples of commercial catches, caught by the eastern sea Danish seine fishery in 2007. The annuli of G. stelleri are formed once a year, with the boundary between opaque and translucent zones forming in September. Relationships between total length (TL) and total weight (TW) were $TW=0.002TL^{3.392}$ ($r^2=0.970$) for females and $TW=0.002TL^{3.335}$ ($r^2=0.961$) for males. TLs at annuli formation in otoliths were back-calculated from the otolith-length relationship and were adjusted to von Bertalanffy growth curves to $L_t=39.71(1-{\exp}^{(-0.152(t+1.156))})$ for females and $L_t=32.16(1-{\exp}^{(-0.213(t+0.879))})$ for males. From the age of 3 years, females grew faster than males (P<0.05).

• Korean Journal of Fisheries and Aquatic Sciences
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• v.48 no.4
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• pp.529-541
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• 2015

We examined the species composition and community structure of demersal fish in the middle and southern coastal waters of the East Sea of Korea by surveying a commercial Danish seine fishery from 2010 to 2013. We considered the waters north of $37^{\circ}N$ as middle and those south of $37^{\circ}N$ as southern waters. A total of 79 demersal fish species belonging to 18 families were collected. Of these, 59 species (77.6%) inhabited only the East Sea as opposed to the West and South Seas of Korea, and most were resident species. The species and biomass were similar between the two portions of the study area. The sandfish Arctoscopus japonicus (78.0%) and the blackfin flounder Glyptocephalus stelleri (65.1%) were the most common species and accounted for the most biomass in the middle and southern waters, respectively. Fish were most abundant at shallow depths (50-100 m) in the middle portion of the East Sea. Using a cluster analysis, we divided the species composition and community structure at the sampling stations into three groups: middle portion (group A), deep area of the southern portion (group B), and shallow areas of the southern portion (group C). A. japonicas and G. stelleri were dominant in groups A and B, while G. stelleri and Clupea pallasii were dominant in group C.