• 한국균학회지
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• 제48권1호
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• pp.55-61
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• 2020

꼬마초롱이끼의 헛뿌리와 가는털깃털이끼의 잎에서 내생균을 분리하였다. 분리된 균주는 형태적 특성과 internal transcribed spacer, large subunit rDNA, Beta-tublin 영역 및 RNA polymerase II second largest subunit gene의 분자적 분석을 토대로 동정하였다. 연구 과정에서 2종의 국내 미기록종 내생균인 Cercophora thailandica와 Biscogniauxia petrensis를 확인하였다. 분리된 미기록종 내생균 균주의 형태적 특성 확인 및 계통 분석 결과에 대해 기술하였다.

• 한국균학회지
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• 제48권2호
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• pp.87-93
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• 2020

A fungal strain designated KNU-WDM2A2 was isolated from mosquitoes in Gimcheon, Korea. The pure culture was transferred to potato dextrose agar (PDA) and synthetic nutrient agar (SNA) media and attained a diameter of 90 mm after 10 days of incubation at 25℃. The colonies were whitish to light pink and cottony to wooly, with an abundant production of aerial mycelia. The strain produced hyaline to slightly yellowish conidiophores that were rough-walled and branched, with conidiogenous cells arising terminally or laterally. Conidia were unicellular, hyaline to light brown, smooth, and oval or ovoid to clavate, with a size of 4.1-6.9×2.5-3.3 ㎛ (n=65). A phylogenetic analysis was conducted using the internal transcribed spacer (ITS) regions and 28S rDNA of large subunit (LSU) sequences, to support the cultural and morphological characteristics. The KNU-WDM2A2 strain was identified here as Biscogniauxia petrensis, new to Korea.