• 생명과학회지
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• 제20권8호
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• pp.1249-1253
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• 2010

Sericin은 실크를 싸고 있는 고분자 수용성 당단백질로서 세포배양에 사용되며 세포분화를 촉진한다. 본 연구의 목적은 갑상선세포(FRTL-5)에서 thyroglobulin (Tg)의 분비에 sericin이 영향을 주는지를 알려고 한다. Sericin에 의해서 Tg의 분비가 촉진되었지만 Tg-mRNA의 발현은 촉진되지 않았다. 이런 상태에서 소포체 샤페론(Bip & calreticulin)과 소포체 막 단백질(IRE1, PERK & ATF6)의 발현이 증가한 것이 확인되었다. 한편 IRE1의 하부 신호전달자인 XBP1의 mRNA splicing 이 약하게 확인되었지만 PERK의 하부 신호전달자인 $eIF2{\alpha}$의 인산화는 일어나지 않았다. 그리고 sericin은 MTT assay 결과 cell viability을 촉진시키는 것도 확인되었다. 위의 결과는 sericin은 재조합 단백질생산에 유용하게 이용될 수 있는 새로운 생체물질로 증명되었다.

• 지질공학
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• 제9권3호
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• pp.187-206
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• 1999

시추공 텔리뷰어 및 BIPS의 공내 검층자료를 이용하여 파쇄매질이 나타내는 투수율텐서를 수치적으로 계산하는 프로그램을 개발하였다. 본 프로그램은 검층자료의 분석을 통해 제공되는 불연속면의 방향과 두께등의 기하학적인 정보를 입력자료로 사용하며, EPM 모델에 근거한 관계식을 적용하여 투수율텐서를 구성하는 9개 성분의 상대적인 값을 계산한다. 거제시 일운면에 위치하는 연구지역 시추공을 대상으로 수리간섭 시험을 실시하여 암반의 이방성을 추정하였으며 BIPS 검층자료로부터 프로그램에 의하여 산정된 이론값과 비교, 분석하여 프로그램의 현장 적용성을 평가하였다. 수리간섭시험을 통하여 추정한 연구지역 암반의 수평적인 이방성비와 주축방향은 2.8 및 $N77^{\circ}CE$인 반면, 프로그램을 적용하여 산정한 값은 3.0 및 $N63^{\circ}CE$로 나타냈다. 두 결과가 불일치한 이유로는 수리간섭시험 해석결과의 신뢰성, EPM모델 및 3승법칙의 현장 적용성, 프로그램에서 가정한 단열의 크기-두께 관계식의 단순화 등의 문제점을 들 수 있다. 개발된 프로그램은 투수율텐서를 계산하는 초기모델로서 단열면의 거칠기, 충전물질의 충전정도 및 수리특성을 반영하는 수리인자의 적용을 통한 개선이 요구되며, 암반의 투수성이 양호하며 시추공 간의 거리가 가까운 연구지역에서 시추공간 수압시험을 실시할 때 3차원의 현장적용성 평가가 가능할 것으로 판단된다.

• The Korean Journal of Physiology and Pharmacology
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• 제11권6호
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• pp.239-246
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• 2007

Expressions of endoplasmic reticulum stress response (ERSR) genes were examined during the neuronal differentiation of rat fetal cortical precursor cells (rCPC) and rat pheochromocytoma PC12 cells. When rCPC were differentiated into neuronal cells for 7 days, early stem cell marker, nest in, expression was decreased from day 4, and neuronal markers such as neurofilament-L, -M and Tuj1 were increased after day 4. In this condition, expressions of BIP, ATF6, and phosphorylated PERK as well as their down stream signaling molecules such as CHOP, ATF4, XBP1, GADD34, Nrf2 and $p58^{IPK}$ were significantly increased, suggesting the induction of ERSR during neuronal differentiation of rCPC. ERSR was also induced during the differentiation of PC12 cells for 9 days with NGF. Neurofilament-L transcript was time-dependently increased. Both mRNA and protein levels of Tuj1 were increased after the induction, and the significant increase in NeuN was observed at day 9. Similar to the expression patterns of neuronal markers, BIP/GRP78 and CHOP mRNAs were highly increased at day 9, and ATF4 mRNA was also increased from day 7. These results strongly suggest the induction and possible role of ERSR in neuronal differentiation process. Further study to identify targets responsible for neuronal induction will be necessary.

• 한국항만학회지
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• 제13권1호
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• pp.51-64
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• 1999

This study is on the focus of the cost-effective and time-effective in the BCTOC which commenced the On Dock Termainal System in 1998 for the first time in Korea. On this process of study two categories should be considered : (1) Improvement on Business Process (2) Improvement on Information Technology. With these two accesses a new future-oriented and prospective model could be put on our hand as follows. (1) Improvement on Business Process \circled1Consistency of container In-Out Operation \circled2Simplicity of Rate appraisal \circled3Unified operation on Billing and Request (2) Improvement on Information Technology \circled1Scale up the Information Application \circled2Constructing an one-spot simultaneous Information Holding System \circled3Combination of Internet and Integrated Database System.

• 동력기계공학회지
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• 제21권1호
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• pp.70-79
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• 2017

The output power of turbine is greatly affected by the losses generated within the passage. In order to develop a better turbine or loss models, an experimental study was conducted using a linear cascade experimental apparatus. The total pressure loss and flow structures were measured at two cross-sectional planes located downstream of blade row. Measurement was conducted in a steady state for the several different locations of the blade row along the rotational direction. The blade row moved by 20 % of the pitch, and tip clearance was varied from 2% to 8%. Axial-type blades were used and its blade chord was 200mm. A square nozzle was applied and its size was $200mm{\times}200mm$. The experiment was conducted at a Reynolds number of $3{\times}10^5$ based on the chord. Nozzle flow angle sets to $65^{\circ}$ based on the axial direction and the solidity of blade row was 1.38. From the experimental results, the total pressure loss was greatly varied in the receding region than in the entering region. The flow properties within the blade passage were strongly changed according to the location of blade row.

• BMB Reports
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• 제45권8호
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• pp.482-487
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• 2012

To identify the novel inhibitors of endoplasmic reticulum stress-induced cell death, we performed a high throughput assay with a chemical library containing a total of 3,280 bioactive small molecules. Cyclosporine A and bromocriptine were identified as potent inhibitors of thapsigargiin-induced cell death (cut-off at $4{\sigma}$ standard score). However, U74389G, the potent inhibitor of lipid peroxidation had lower activity in inhibiting cell death. The inhibition effect of cyclosporine A and bromocriptine was specific for only thapsigargin-induced cell death. The mechanism of inhibition by these compounds was identified as modification of the expression of glucose regulated protein-78 (GRP-78/Bip) and inhibition of phosphorylation of p38 mitogen activated protein kinase (MAPK). However, these compounds did not inhibit the same events triggered by tunicamycin, which was in agreement with the cell survival data. We suggest that the induction of protective unfolded protein response by these compounds confers resistance to cell death. In summary, we identified compounds that may provide insights on cell death mechanisms stimulated by ER stress.

• 한국환경복원기술학회지
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• 제9권3호
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• pp.39-50
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• 2006

In this study, the water quality investigated during 2002s to 2004s for Anyang river and its affluent that was based on biological and chemical methods as well as ecological index of each streams. The improving of water quality and the multiplicity of specious in the most streams except for Anyang, which was due to the improving of water quality and water resource. The seasonal dry states are completely disappeared, but water quality of Anyang main stream was classified as fifth grades water with BIP 8.51~10.00 and BOD 8.16~14.4mg/l in Anyang stream overall. And alpha-mesosaprobic in upper, mid parts and polysaprobic in down parts of Anyang main stream are appeared according to Saprobien system, respectively. The water supply of treated sewage is appeared that upgrading effects of water quality in Anyang main stream and affluents of Anyang city area, but the practical effects of "Saving Anyang River" could not gained because the water quality of other branches in upstream parts than upstream measuring point of Anyang main stream, Wanggok stream Sanbon stream in Gunpo and Eeiwang cities, was not improved.

• 대한지질공학회:학술대회논문집
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• 대한지질공학회 2005년도 정기총회 및 학술발표회
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• pp.157-164
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• 2005

BIPS와 Televiewer를 이용하여 10개의 지하수 관측공에서 획득된 1,591개 불연속면을 분석하여 지하수 유동과의 관계를 비교${\cdot}$분석하였다. 연구지역 내의 지형은 산계가 잘 발달된 계곡형 지형이며, 수계의 발달은 매우 미약하다. 연구지역 주변에는 북북동-남남서 방향의 양산단층과 동래단층이 발달되어 있으며, 연구지역 내에는 EW방향의 선구조도 발달되어 있다. 지하수공내에서 조사된 불연속면의 주 방향은 위성사진에서 판독된 선구조의 방향과 대부분 일치한다. 지구통계기법을 이용하여 작성된 지하수위 등고선도에서 추정된 지하수 유동방향과 유향${\cdot}$유속기에서 측정된 지하수 유향은 거의 일치하지만, 단열암반에 발달된 불연속면의 방향과 지하수 유동방향은 2가지로 해석되었다. 첫째는 단열암반에 발달된 불연속면의 방향과 지하수 유동방향이 잘 일치하는 경우(BH-01, 03, 04, 12공), 둘째는 불연속면의 방향과 지하수 유동방향이 잘 일치하지 않는 경우(BH-02, 05, 07, 11) 이다. 지하수 유동방향이 불연속면의 방향과 일치하지 않는 경우는 지형의 변화에 더 영향을 받는 것으로 나타났다. 따라서 단열암반 내에서 지하수의 유동은 단열의 방향성에 영향을 받지만, 지형의 특성에도 큰 영향을 받는다.

• Clinical and Experimental Reproductive Medicine
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• 제42권1호
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• pp.1-7
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• 2015

Stress coping mechanisms are critical to minimize or overcome damage caused by ever changing environmental conditions. They are designed to promote cell survival. The unfolded protein response (UPR) pathway is mobilized in response to the accumulation of unfolded proteins, ultimately in order to regain endoplasmic reticulum (ER) homeostasis. Various elements of coping responses to ER stress including Perk, Ask1, Bip, Chop, Gadd34, Ire1, Atf4, Atf6, and Xbp1 have been identified and were found to be inducible in oocytes and preimplantation embryos, suggesting that, as a normal part of the cellular adaptive mechanism, these coping responses, including the UPR, play a pivotal role in the development of preimplantation embryos. As such, the UPR-associated molecules and pathways may become useful markers for the potential diagnosis of stress conditions for preimplantation embryos. After implantation, ER stress-induced coping responses become physiologically important for a normal decidual response, placentation, and early organogenesis. Attenuation of ER stress coping responses by tauroursodeoxycholate and salubrinal was effective for prevention of cell death of cultured embryos. Further elucidation of new and relevant ER stress coping responses in periimplantation embryos might contribute to a comprehensive understanding of the regulation of normal development of embryonic development and potentiation of embryonic development in vitro.

• 한국동물학회지
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• 제35권4호
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• pp.456-465
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• 1992

The 94 KDa glucose-resulsted Protein (SH 94), one of stress Proteins, is a Ca2+-binding protein in the endoplasmic reticulum (ER). In this study, the possible effect of Ca2+ on the native conformation of grp 94 was examined. When the purified grp 94 was analyzed by Sel filtration in the presence of either EGTA or CaCl2, it was eluted with apparent molecular weight (MW) of 100 KDa in both cases. When similarly analyzed with microtome or cell Ivsate, however, srp 94 was eluted with apparent IW of 200 KDa in the presence of E6TA, while with apparent MW of 100 KDa in the presence of CaCl2, indicating possible association of grp 94 with one or more other proteins in the absence of CaCl2. Consequently, immunoprecipitation with anti-grp 94 was carried out to determine which proteins specifically interact with grp 94. It is sho%un that srp 94 may interact, in a Ca2+_dependent manner. with other proteins including BiP (grp 78) which is also a stress protein in the ER.