• 한국환경보건학회지
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• 제40권3호
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• pp.187-203
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• 2014

Objectives: As the demand to deal with pathogens in domestic research institutions has expanded and biological accidents have increased, the need for systematic biosafety management in infectious disease laboratories has grown. According to international standards, risk assessment (RA) is required for biosafety management. However, RA criteria have not been clearly established in Korea, so to this end I have attempted to determine RA criteria meeting international levels Methods: In order to provide RA criteria for application, I analyzed the RA criteria in use in the U.S., Europe and at international organizations. In order to ensure the public nature of the RA criteria, I constructed the research model through modified management consulting methodology reflecting the model of Radnor and O'Mahoney. Results: According to the results of the study, existing laboratory biosafety regulations were comparable to domestic laboratory safety laws. Existing laboratory biosafety standards that are designed around risk factors were found to be insufficient. An RA case to be carried out in infectious disease laboratories at the National Institute of Health of KCDC was identified. Conclusion: To establish a systematic risk management system meeting international standards, it was necessary first to harmonize the systems of national and international standards. In addition, in order to provide specific biosafety management on-site, I recognized a need for methodology and planning strategies to discover biosafety management so that it can be carried out as required through the RA of individual laboratories.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2005년도 춘계 국제심포지엄 및 학술대회
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• pp.133-133
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• 2005

• 대한임상검사과학회지
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• 제50권1호
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• pp.71-76
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• 2018

본 연구는 2017년 1월 3일~3월 25일까지 실험실 연구자를 대상으로 생물 안전성에 대한 현황 연구를 목적으로 한다. 국내 감염병 병원체 취급대학 및 의과대학 소속 실험실에서 병원성 미생물을 취급하는 실험실 연구자 총 500명을 설문 조사하여 실시되었다. 조사 결과에 따르면 응답자는 높은 수준의 인지도를 보였으나 전반적인 생물 안전성 규칙 및 규정을 준수하지는 않았다. 실험실의 생물안전 시설의 등급 여부에 관한 문항에 관한 질문에는 '알고 있다'가 55.8%,'모른다'가 44.2%로 나타났다. 연구자들은 적절한 안전 조치가 개인을 보호 할 수 있으며, 안전 장비와 생물 안전 계획이 충분하지 않음에도 실험실이 안전하다고 믿었다. 생물안전 교육에 관련된 조사에서 연구자의 80%는 실험실 안전에 대한 교육을 받은 경험이 있었고 20%는 안전교육을 받은 적이 없었다. 생물안전교육에 대한 필요성은 66%, 교육내용에 대한 만족도는 46%로 나타났다. 이러한 결과는 연구자들은 이미 생물안전교육을 받은 경험이 있지만 지속적인 교육이 필요한 것으로 생각하였다. 또한 생물 안전성 수준을 향상시키는데 있어 가장 중요한 것은 훈련 프로그램 및 교육시스템을 강화하는 것이라는 의견이 있었다. 결론적으로 실험실에서 일어날 수 있는 위험에 대비하기 위한 실험실 생물 안전에 대한 보다 개선된 교육 시스템을 개발할 필요가 있다.

• 환경영향평가
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• 제22권2호
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• pp.125-134
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• 2013

Plant virus coat (CP) gene-mediated protection is one of the best known approaches to protect against virus resistant transgenic plants. Transgenic N. benthamiana plants containing the CP gene of Zucchini green mottle mosaic virus (ZGMMV) were used for the environmental risk assessment of the living modified (LM) plants with plant virus resistance. The most optimal co-infection method of both ZGMMV and CMV (Cucumber mosaic virus) on Non-LM and CP-expressing LM tobacco plants was established and co-infection of CMV and ZGMMV was confirmed by polymerase chain reaction (PCR). To address the effects of LM tobacco plants on the mutation of the virus, in-vitro transcripts of CP and Replicase (Rep) derived from CMV and/or ZGMMV were inoculated onto Non-LM or LM tobacco plants. Mutation frequency of CP and Rep from CMV and ZGMMV was examined through six serial passages in Non-LM and LM tobacco plants. Little actual frequency of mutation was estimated, probably due to the limited number of transgenic plants tested in this study. However, it does not suggest environmental safety of these CP-mediated LM plants. Further study at a larger scale is needed to evaluate the environmental risk associated with the CP-expressing LM plants.

• Journal of Preventive Medicine and Public Health
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• 제38권4호
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• pp.449-456
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• 2005

Objectives : The biosafety level (BSL) practiced in microbiology laboratories in Korea according to the laboratory biosafety manual published by the World Health Organization (WHO) was evaluated using the data obtained by a survey. Methods : Under the advise of Clinical Laboratory Physicians, 144 types of microorganisms were screened based on the guidelines of biosafety in microbiological and biomedical laboratories published by the US Center for Disease Control and Prevention and classified into 1-4 risk groups. A questionnaire containing 21 questions in 5 areas was developed using the biosafety manual by published WHO. Of the 1,876 different organizations sent the survey, 563 responded to the survey (response rate: 30.0%). The species of microoganisms handled by as well as the biosafety level in microbiology laboratories were analyzed. Results : There were 123 species of microorganisms handled in microbiology labs in Korea. The BSL required in 512 microbiology labs was answered by the survey responders as the first grade in 33 labs (6.4%), 2nd in 437 (85.4%), 3rd in 42 (8.2%), and 4th in none. The average number of items satisfied was 12.2, showing only a 57.9% satisfactory rate and normal distribution. Conclusions : The state of overall observance of BSL in most microbiology labs of Korea was evaluated as lagging compared with the standard set up by WHO. Therefore, the Korean government need to produce and distribute a biosafety manual in microbiology laboratories and make efforts to prevent this threat through measures such as training in biosafety in microbiology labs.

• 한국동물위생학회지
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• 제28권4호
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• pp.367-373
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• 2005

Coccidiosis of domestic fowl is a parasitic disease that infects poultry drastically and can cost the pastured poultry producer much in the course of a year. Two hundred forty, day-old-broiler chicks were purchased and were randomly divided in eight groups, containing thirty birds in each group. Decoquinate $6\%$, maduramicin ammonium $2\%$, monensin sodium $13.2\%$, salinomycin sodium $12\%$, a live attenuated vaccine, and trivalent live attenuated vaccine, was provided to the day chicks of six groups, respectively. The chicks of last two groups served as infected non-medicated and uninfected non-medicated, respectively. Feed consumption, weight gain, feed conversion ratio, mortality and oocyst count per gram feces were recorded during the conduction of the experiment. Among treated groups, performance of salinomycin group was significantly better (p<0.05) in all aspects. Salinomycin acts against the sporozoites, trophozoites and first generation schizonts and is highly effective against the economically important species of Eimeria. The present study confirms that use of salinomycin, as an anticoccidial, is a drug of choice.

• Journal of Plant Biotechnology
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• 제48권4호
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• pp.201-206
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• 2021

우리나라는 유전자변형생물체(GMO)의 개발 및 수입 등에 관한 안전성 확보를 위하여 LMO법을 제정하였다. 이에 따라 국내에서 GMO가 식품 및 사료용으로 수입, 생산될 경우는 OECD가 제시한 '실질적 동등성' 개념에 따라 안전성 심사를 한다. 안전성 심사에는 GMO에 대한 알레르기 평가심사가 포함된다. 알레르기성 평가 방법은 OECD, FAO, WHO 등 다양한 국제기구를 통해 논의되어왔다. 주요 알레르기성 평가 방법으로는 GMO에서 새로 발현된 단백질의 물리 화학적 감수성 확인, 기존 알레르기 단백질과의 아미노산 서열 유사성 분석, 혈청 스크리닝 등이 있다. 따라서 본 연구에서는 유전자변형생물체의 알레르기성 평가를 위한 가이드라인과 관련 연구들을 소개하였다.

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2005년도 Proceedings of The 2nd Asian Society of Veterinary Pathology Symposium(Vol.2) and 2005 Annual Meeting of The Korean Society of Veterinary Pathology(Vol.9)
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• pp.62.1-63
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• 2005

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2005년도 Proceedings of The 2nd Asian Society of Veterinary Pathology Symposium(Vol.2) and 2005 Annual Meeting of The Korean Society of Veterinary Pathology(Vol.9)
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• pp.85-85
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• 2005

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2005년도 Proceedings of The 2nd Asian Society of Veterinary Pathology Symposium(Vol.2) and 2005 Annual Meeting of The Korean Society of Veterinary Pathology(Vol.9)
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• pp.86-87
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• 2005