• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.870-870
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• 2005

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.884-884
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• 2005

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.910-910
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• 2005

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.295-295
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• 2005

• Journal of the Korean Magnetic Resonance Society
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• v.22 no.4
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• pp.91-100
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• 2018

Benzisothiazolinone (BIT) is the preservative that is widely used in industrial and household products. In this study, zebrafish (Danio rerio) was exposed to BIT at different concentrations (control, 0.5 g/L, 1.0 g/L and 2.0 g/L) for 72 hours. The techniques of nuclear magnetic resonance (NMR) spectroscopy were applied to analyze the effects of BIT on zebrafish. The advantages of NMR are the minimal sample preparation and high reproducibility of experimental results. With the multivariate statistical analysis, dimethylamine, N-acetylaspartate, glycine and histidine were identified as an important metabolite in differentiating between the control and BIT-exposed group. This study will improve the understanding the metabolite changes in the zebrafish in response to BIT exposure.

• Proceedings of the Korea Crystallographic Association Conference
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• 2000.11a
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• pp.8-8
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• 2000

• Proceedings of the Zoological Society Korea Conference
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• 2000.10a
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• pp.231.2-231
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• 2000

No Abstract, See Full Text

• The Korean Journal of Physiology and Pharmacology
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• v.2 no.5
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• pp.541-547
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• 1998

• The Korean Journal of Physiology and Pharmacology
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• v.8 no.4
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• pp.201-206
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• 2004

Mitochondrial ATP-sensitive potassium $(mitoK_{ATP})$ channels play a role in early and late ischemic preconditioning. Nevertheless, the subunit composition of $mitoK_{ATP}$ channels remains unclear. In this study, we investigated the subunit composition of $mitoK_{ATP}$ channels in mitochondria isolated from rat cardiac myocytes. Mitochondria were visualized using the red fluorescence probe, Mitrotracker Red, while $mitoK_{ATP}$ channels were visualized using the green fluorescence probe, glibenclamide-BODIPY. The immunofluorescence confocal microscopy revealed the presence of Kir6.1, Kir6.2 and SUR2 present in the cardiac mitochondria. Western blot analysis was carried to further investigate the nature of $mitoK_{ATP}$ channels. For SUR proteins, a 140-kDa immunoreactive band that corresponded to SUR2, but no SUR1 was detected. For Kir6.2, three bands $({\sim}44,\;{\sim}46,\;and\;{\sim}30\;kDa)$ were detected, and a specific ${\sim}46-kDa$ immunoreactive band corresponding to Kir6.1 was also observed. These observations suggest that the subunits of $mitoK_{ATP}$ channels in rat myocytes include Kir6.1, Kir6.2, and a SUR2-related sulfonylurea-binding protein.

• Proceedings of the Microbiological Society of Korea Conference
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• 2005.05a
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• pp.213.4-213
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• 2005