• 약학회지
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• 제55권1호
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• pp.56-63
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• 2011

Fabry disease (FD) is an X-linked inborn error of glycoshpingolipid metabolism resulting from mutation in the enzyme ${\alpha}$-galactosidase A gene. The disease is an X-linked lipid storage disorder and the lack of ${\alpha}$-Gal A causes an intracellular accumulation of glycosphingolipids, mainly globotriaosylceramide (Gb-3). Measurement of Gb-3 in plasma has clinical importance for monitoring after enzyme replacement therapy for confirmed FD patients. Using electrospray ionization MS/MS we had developed, a simple, rapid, and highly sensitive analytical method for Gb-3 in plasma was used for the purpose of screening FD among high risk groups in Korean population. To date, no comprehensive results for FD screening have been performed and reported in Korea. We screened 1,100 outpatients from 13 hospitals (including clinics) to assess the incidence of FD among patients in high risk groups. For patients with borderline level amount of Gb-3, we repeated Gb-3 or performing complementary or confirmative assay with ${\alpha}$-Gal A activity and DNA mutaion analysis for confirmation diagnosis. Of 1,100 we diagnosed 3 FD with 2 classical type and 1 carrier (0.27%).

• 대한의용생체공학회:의공학회지
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• 제22권4호
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• pp.349-358
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• 2001

A novel electro-mechanical implantable ventricular assist system is developed as a bridge to transplantation or recovery for patients with end-stage heart failure. The developed system is composed of an implanted blood pump, an external monitoring system which stores data, and a wearable system including a portable external driver and a portable power supply system. The blood pump is designed to be implanted into the left upper abdominal space and provides blood flow from the left ventricular apex to the aorta. The pulsatile blood flow is generated by a double cylindrical cam. There was mo excessive heat emission from the blood pump into the temperature-controlled chamber in the heat test and no stagnated flow within the blood sac by the observation in the flow visualization test. Animal experiments were performed using sheep and calves. The maximum assist flow rate reached 7.85L/min in the animal experiment. The evaluation results showed that the developed system was feasible for the implantable ventricular assist system. The long-term in vitro durability test and mid-term in vivo experiments are in progress and mow the modified next model is under development.

• 대한의용생체공학회:의공학회지
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• 제18권4호
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• pp.477-484
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• 1997

본 논문에서는 각성도 평가시 초기 각성상태 차이에 따른 보상방법을 제안하고 보상상수를 결정하였다. 각성도는 IRI와 Nz에 의하여 측정되었다. BI는 초기 저각성상태의 영향을 많이 받게 되며 이는 각성도의 지표인 Nz 값에 영향을 주게 되어 각성수준이 실제보다 높게 평가된다. 따라서 본 연구에서는 BI의 과대평가로 인한 오판정 문제를 해결하기 위하여 수정된KSS 측도(mKSS)를 이용한 보상법을 제안하고 각성수준에 따른 수정된 KSS 측도에 대응하는 보상상수를 결정하였다. 이 보상상수를 개발된 휴대형 각성도 측정 시스템에 적용하여, 실시간으로 각성도를 평가 및 제어한 결과, 본 연구에서 제안한 보상법 및 보상상수의 유용성을 확인하였다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2003년도 하계학술대회 논문집 D
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• pp.2807-2808
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• 2003

말초 신경계에서 손상된 신경은 그 손상된 정도에 따라 재생의 정도가 달라진다. 이러한 신경 재생의 정도를 확인할 수 있는 방법으로 초미세 전극 어레이 기술이 연구되고 있는데 이것은 전기신호경로(via hole)를 초미세 전극어레이를 제작하여, 손상된 신경의 근위부(proximal stump)와 원위부(distal stump) 사이에 직접 삽입하여 선경으로부터 재생되어온 신경 섬유의 전기적 전도 선호를 측정 및 기록하는 것이다. 이는 신경 재생을 관찰하는 방법으로서 신경보철(neural prostheses) 기술에 이용된다. 따라서, 본 논문에서는 손상된 신경의 재생을 관찰하기 위한 implantable microelectrode array system을 설계하고, 원 신호인 선경 전도 신호의 특성과 제작된 이식형 초미세전극(implantable microelectrode)의 특성을 고찰하며, 동물 실험을 통하여 그 성능을 검증하였다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2005년도 추계학술대회 논문집
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• pp.77-80
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• 2005

Local probe techniques such as scanning probe microscopy (SPM) or atomic force microscopy (AFM) extended our perception into ultra small world. Specially, the sense of touching was extended by AFM into the micro- and nanoworld and has provided complementary new insights of the microscopic world. In addition, touching objects is an essential step before trying to manipulate things. SPM as a touch sensor not only measure the mechanical properties but also detect different properties such as magnetic, electrical, ionic, thermal, chemical and biophysical properties in nanoscale and even less. Obtaining biophysical measurements, monitoring dynamics and processes together with high-resolution imaging of the biomolecules and cells with rather simpler sample preparation than any other techniques give great attractions to the scientists experimenting with biological samples. Among the many AFM capabilities we will specifically introduce the force plot which is used to measure tip-sample interactions and its application this time.

• 대한의용생체공학회:의공학회지
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• 제22권1호
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• pp.81-90
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• 2001

본 연구에서는 디지털 영상 뇌파계(digital video electroencephalogram, Digital VEEG)에서 비디오 영상과 뇌전도 파형의 동기화된 편집 시스템을 구성한다. 이 시스템은 기존 아날로그 영상 뇌파계(analog video electroencephalogram)의 동기화 문제와 디지털 영상 시스템에서의 영상편집 문제를 해결하기 위하여 MPEG-I(이하 MPEG) 고압축 기술을 이용한 MPEG 인코딩 보드(encoding board)와 MPEG 편집 엔진(editing engine)을 각각 사용하였다. 시스템은 디지털 영상뇌파계모듈과 디지털 편집 모듈로 구성되며, 뇌전도모듈에서는 환자에게 연결된 전극을 통해 들어온 뇌파를 생체신호증폭기를 이용하여 증폭한 후 AD 보드(analog to digital board)를 이용 디지털화한다. 디지털 카메라로 촬영된 환자영상의 아날로그 영상신호(NTSC 신호)는 MPEG 인코딩 보드를 이용하여 고압축 디지털화한다. 이후 디지털화된 뇌전도신호와 MPEG 형식의 영상을 시간 동기화하여 두 개의 모니터에 각각보여준다. 편집 모듈에서는 영상신호와 뇌파신호를 어느 부분이든 간단한 조작으로 오려 붙이기(cut and paste) 기능을 이용할 수 있다. 본 시스템은 사용된 데이터 모두 디지털 기술을 이용하여 영상과 뇌파신호의 정확한 동기화 및 각각의 데이터의 오려 붙이기 기능을 가능케 하였으며, 이는 환자의 데이터를 관리 및 보관하는데 있어, 임상의에게 의미 있는 자료만을 모아서 효율적으로 관리할 수 있게 해준다. 이와 같은 장점을 갖는 디지 영상뇌파계 편집시스템을 구현하였다.

• Biomedical Engineering Letters
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• 제8권4호
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• pp.373-382
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• 2018

Since epileptic seizure is unpredictable and paroxysmal, an automatic system for seizure detecting could be of great significance and assistance to patients and medical staff. In this paper, a novel method is proposed for multichannel patient-specific seizure detection applying the earth mover's distance (EMD) in scalp EEG. Firstly, the wavelet decomposition is executed to the original EEGs with five scales, the scale 3, 4 and 5 are selected and transformed into histograms and afterwards the distances between histograms in pairs are computed applying the earth mover's distance as effective features. Then, the EMD features are sent to the classifier based on the Bayesian linear discriminant analysis (BLDA) for classification, and an efficient postprocessing procedure is applied to improve the detection system precision, finally. To evaluate the performance of the proposed method, the CHB-MIT scalp EEG database with 958 h EEG recordings from 23 epileptic patients is used and a relatively satisfactory detection rate is achieved with the average sensitivity of 95.65% and false detection rate of 0.68/h. The good performance of this algorithm indicates the potential application for seizure monitoring in clinical practice.

• Biomedical Engineering Letters
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• 제8권4호
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• pp.345-353
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• 2018

This study suggested a new EMG-signal-based evaluation method for knee rehabilitation that provides not only fragmentary information like muscle power but also in-depth information like muscle fatigue in the field of rehabilitation which it has not been applied to. In our experiment, nine healthy subjects performed straight leg raise exercises which are widely performed for knee rehabilitation. During the exercises, we recorded the joint angle of the leg and EMG signals from four prime movers of the leg: rectus femoris (RFM), vastus lateralis, vastus medialis, and biceps femoris (BFLH). We extracted two parameters to estimate muscle fatigue from the EMG signals, the zero-crossing rate (ZCR) and amplitude of muscle tension (AMT) that can quantitatively assess muscle fatigue from EMG signals. We found a decrease in the ZCR for the RFM and the BFLH in the muscle fatigue condition for most of the subjects. Also, we found increases in the AMT for the RFM and the BFLH. Based on the results, we quantitatively confirmed that in the state of muscle fatigue, the ZCR shows a decreasing trend whereas the AMT shows an increasing trend. Our results show that both the ZCR and AMT are useful parameters for characterizing the EMG signals in the muscle fatigue condition. In addition, our proposed methods are expected to be useful for developing a navigation system for knee rehabilitation exercises by evaluating the two parameters in two-dimensional parameter space.

• Parasites, Hosts and Diseases
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• 제59권3호
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• pp.319-324
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• 2021

Incidence of tsutsugamushi disease (scrub typhus) caused by Orientia tsutsugamushi, is steadily increasing. It is a mite-borne disease transmitted by chigger mites. In this study, the chigger mites were collected from field small mammals in Hwaseong-si (city), Gyeonggi-do (province), Korea, 2019 and 2020. The field small mammals captured were 56 Apodemus agrarius (94.9%) and 3 Crocidura lasiura (5.1%). A total of 7,531 chigger mites were collected from the captured small mammals. Using PCR test, 153 chigger mite pools were examined and 17 pools were reported positive for O. tsutsugamushi. The O. tsutsugamushi were identified to 5 strains; Jecheon strain was most prevalent, followed by Boryong strain. The other strains were OI011, Taguchi, and Shimokoshi. Collectively, these results provide essential regional information on mite-borne tsutsugamushi disease in the Hwaseong-si, and further contribute to bring awareness and rapid diagnosis for the tsutsugamushi disease.

• Tuberculosis and Respiratory Diseases
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• 제85권2호
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• pp.111-121
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• 2022

Multidrug-resistant tuberculosis (MDR-TB) is caused by an organism that is resistant to both rifampicin and isoniazid. Extensively drug-resistant TB, a rare type of MDR-TB, is caused by an organism that is resistant to quinolone and one of group A TB drugs (i.e., linezolid and bedaquiline). In 2018, the World Health Organization revised the groupings of TB medicines and reclassified linezolid as a group A drug for the treatment of MDR-TB. Linezolid is a synthetic antimicrobial agent in the oxazolidinone class. Although linezolid has a good efficacy, it can cause substantial adverse events, especially hematologic toxicity. In both TB infection and linezolid mechanism of action, mitochondrial dysfunction plays an important role. In this concise review, characteristics of linezolid as an anti-TB drug are summarized, including its efficacy, pathogenesis of hematologic toxicity highlighting mitochondrial dysfunction, and the monitoring and management of hematologic toxicity.