• Journal of Marine Bioscience and Biotechnology
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• v.16 no.1
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• pp.36-44
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• 2024

This study attempted to improve the growth of the freshwater microalgae, Parachlorella kessleri, through the sequential optimization of culture conditions. This attempt aimed to enhance the microalgae's ability to fixate atmospheric CO₂. Culture temperature and light intensity appropriate for microalgal growth were scanned using a high-throughput photobioreactor system. The supplied air flow rate varied from 0.05 to 0.3 vvm, and its effect on the growth rate of P. kessleri was determined. Next, sodium phosphate buffer was added to the culture medium (BG11) to enhance CO₂ fixation by increasing the availability of CO₂(HCO₃^-) in the culture medium. The results indicated that optimal culture temperature and light intensity were 20℃-25℃ and 300 μE/m²/s, respectively. Growth rates of P. kessleri under various air flow rates highly depended on the increase of the culture's flow rate and pH which determines CO₂ availability. Adding sodium phosphate buffer to BG11 to maintain a constant neutral pH (7.0) improved microalgal growth compared to control conditions (BG11 without sodium phosphate). These results indicate that the CO₂ fixation rate in the air could be enhanced via the sequential optimization of microalgal culture conditions.

• Journal of Korean Society of Forest Science
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• v.110 no.2
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• pp.217-232
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• 2021

This study calculated the productivity and cost of extraction and processing of logging residues by cut-to-length (CTL) and whole-tree (WT) harvesting methods. In addition, the comparative analysis of the characteristics of wood chip fuel to examine whether it was suitable for the fuel conditions of the energy facility. In the harvesting and processing system to produce the wood chips of logging residues the system productivity and cost of the CTL harvesting system were 1.6 Gwt/SMH and 89,865 won/Gwt, respectively. The productivity and cost of the WT harvesting system were 2.9 Gwt/SMH and 72,974 won/Gwt, respectively. The WT harvesting productivity increased 1.3times while harvesting cost decreased by 18.7% compared to the CTL harvesting system. The logging residues of wood chips were not suitable for CTL wood chips based on International Organization for Standardization (ISO 17225-4:2021) and South Korea standard (NIFoS, 2020), but the quality (A2, Second class) was improved through screening operation. The WT-unscreened wood chips conformed to NIFoS standard (second class) and did not conform to ISO but were improved through screening operation (Second class). In addition to the energy facility in plant A, all wood chips except CTL-unscreened wood chips were available through drying processing. The WT-unscreened wood chips were the lowest at 99,408 won/Gwt. Plants B, C, and D had higher moisture content than plant A, so WT-unscreened wood chips without drying processing were the lowest at 57,204 won/Gwt. Therefore, the production of logging residues should improve with operation methods that improve the quality of wood chips required for applying the variable biomass and energy facility.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.8-9
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• 2017

Soybean yield has been low and unstable in Japan and other areas in East Asia, despite long history of cultivation. This is contrasting with consistent increase of yield in North and South America. This presentation tries to describe perspective of breaking stagnation of soybean yield in East Asia, considering the factors of the different yields between regions. Large amount of rainfall with occasional dry-spell in the summer is a nature of monsoon climate and as frequently stated excess water is the factor of low and unstable soybean yield. For example, there exists a great deal of field-to-field variation in yield of 'Tanbaguro' soybean, which is reputed for high market value and thus cultivated intensively and this results in low average yield. According to our field survey, a major portion of yield variation occurs in early growth period. Soybean production on drained paddy fields is also vulnerable to drought stress after flowering. An analysis at the above study site demonstrated a substantial field-to-field variation of canopy transpiration activity in the mid-summer, but the variation of pod-set was not as large as that of early growth. As frequently mentioned by the contest winners of good practice farming, avoidance of excess water problem in the early growth period is of greatest importance. A series of technological development took place in Japan in crop management for stable crop establishment and growth, that includes seed-bed preparation with ridge and/or chisel ploughing, adjustment of seed moisture content, seed treatment with mancozeb+metalaxyl and the water table control system, FOEAS. A unique success is seen in the tidal swamp area in South Sumatra with the Saturated Soil Culture (SSC), which is for managing acidity problem of pyrite soils. In 2016, an average yield of $2.4tha^{-1}$ was recorded for a 450 ha area with SSC (Ghulamahdi 2017, personal communication). This is a sort of raised bed culture and thus the moisture condition is kept markedly stable during growth period. For genetic control, too, many attempts are on-going for better emergence and plant growth after emergence under excess water. There seems to exist two aspects of excess water resistance, one related to phytophthora resistance and the other with better growth under excess water. The improvement for the latter is particularly challenging and genomic approach is expected to be effectively utilized. The crop model simulation would estimate/evaluate the impact of environmental and genetic factors. But comprehensive crop models for soybean are mainly for cultivations on upland fields and crop response to excess water is not fully accounted for. A soybean model for production on drained paddy fields under monsoon climate is demanded to coordinate technological development under changing climate. We recently recognized that the yield potential of recent US cultivars is greater than that of Japanese cultivars and this also may be responsible for different yield trends. Cultivar comparisons proved that higher yields are associated with greater biomass production specifically during early seed filling, in which high and well sustained activity of leaf gas exchange is related. In fact, the leaf stomatal conductance is considered to have been improved during last a couple of decades in the USA through selections for high yield in several crop species. It is suspected that priority to product quality of soybean as food crop, especially large seed size in Japan, did not allow efficient improvement of productivity. We also recently found a substantial variation of yielding performance under an environment of Indonesia among divergent cultivars from tropical and temperate regions through in a part biomass productivity. Gas exchange activity again seems to be involved. Unlike in North America where transpiration adjustment is considered necessary to avoid terminal drought, under the monsoon climate with wet summer plants with higher activity of gas exchange than current level might be advantageous. In order to explore higher or better-adjusted canopy function, the methodological development is demanded for canopy-level evaluation of transpiration activity. The stagnation of soybean yield would be broken through controlling variable water environment and breeding efforts to improve the quality-oriented cultivars for stable and high yield.

• Korean Journal of Environmental Biology
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• v.34 no.4
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• pp.257-263
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• 2016

Objective of this study was to evaluate the carbon budget on 40 years old pear orchard at Naju. For carbon budget assessment, we measured the soil respiration, net ecosystem productivity of herbs, pear biomass and net ecosystem exchange. In 2015, pear orchard released about $25.6ton\;CO_2\;ha^{-1}$ by soil respiration. And $27.9ton\;CO_2\;ha^{-1}$ was sequestrated by biomass growth. Also about $12.6ton\;CO_2\;ha^{-1}$ was stored at pruning branches and about $5.2ton\;CO_2\;ha^{-1}$ for photosynthesis of herbs. As a result, 25.6 ton of $CO_2$ per ha is annually released to atmosphere. At the same time about 45.7 ton of $CO_2$ was sequestrated from atmosphere. When it sum up the amount of $CO_2$ release and sequestration, approximately $20.1ton\;CO_2\;ha^{-1}$ was sequestrated by pear orchard in 2015, and it showed no significant differences with net ecosystem exchanges ($17.8ton\;CO_2\;ha^{-1}\;yr^{-1}$) by eddy covariance method with the same period. Continuous research using various techniques will help the understanding of $CO_2$ dynamics in agroecosystem and it can be able to present a new methodology for assessment of carbon budget in woody crop field. Futhermore, it is expected that the this study can be used as the basic data to be recognized as a carbon sink.

• Korean Journal of Environmental Biology
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• v.32 no.1
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• pp.58-67
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• 2014

The outdoor mass cultivation is not possible for microalgae in Korea all year round, due to cold winter season. It is not easy to maintain proper level of productivity of microalgae even in winter. To prevent a drastic decrease of temperature in a greenhouse, two layers were covered additionally, inside the original plastic layer of the greenhouse. The middle layer was made up of plastic and the inner layer, of non-woven fabric. Acrylic transparent bioreactors were constructed to get more sunlight, not only from the upper side but also from the lateral and bottom directions. In winter at freezing temperatures, six different culture conditions were compared in the triply covered, insulated greenhouse. Wastewater after anaerobic digestion was used for the cultivation of microalgae to minimize the production cost. Water temperature in the bioreactors remained above $10^{\circ}C$ on average, even without any external heating system, proving that the triple-layered greenhouse is effective in keeping heat. Algal biomass reached to 0.37g $L^{-1}$ with the highest temperature, in the experimental group of light-reflection board at the bottom, with nitrogen and phosphorus removal rate of 92% and 99%, respectively. When fatty acid composition was analyzed using gas-chromatography, linoleate (C18 : 3n3) occupied the highest proportion up to 61%, in the all experiment groups. Chemical oxygen demand (COD), however, did not decrease during the cultivation, but rather increased. Although the algal biomass productivity was not comparable to warm seasons, it was possible to maintain water temperature for algae cultivation even in the coldest season, at the minimum cost.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.6
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• pp.460-466
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• 2009

Carbon fixation and density of crops are important to estimate carbon uptake or emission by agricultural production activities and to establish life cycle inventory of crops for assessment of climate change impact. In this study, regional carbon fixation and density in each part of 14 crops, harvest index, and ratio of aboveground to underground were investigated to estimate biomass of 14 crops in Korea by using agricultural statistics data. Biomass yield of potato was $16.5ton\;ha^{-1}$, which was the highest, and those of rice, sweet potato, and garlic were $10.5ton\;ha^{-1}$, $8.7ton\;ha^{-1}$, and $7.5ton\;ha^{-1}$ respectively. Biomass yield of Green onion was the lowest as $2.8ton\;ha^{-1}$. Carbon density of 14 crops were in the order of potato ($6.4ton\;ha^{-1}$), rice ($4.2ton\;ha^{-1}$), sweet potato ($3.4ton\;ha^{-1}$), rape ($2.9ton\;ha^{-1}$) and garlic ($2.8ton\;ha^{-1}$). Regional distribution of carbon contents for each crop mapped revealed that carbon fixation of rice, soybean, sesame, garlic, and green onion were the highest in Jeonnam province, barley, red pepper, and watermelon in Gyeongnam, perilla in Chungnam, peanut in Gyeongbuk, rape and carrot in Jeju, sweet potato in Gyeonggi, potato in Gangwon. The results can be applied for assessing life cycle inventory of crops and crop productivity using remotely sensed data.

• Korean Journal of Environmental Biology
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• v.18 no.1
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• pp.77-93
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• 2000

Field survey on the spatio-temporal distribution of water quality and chlorophyll a concentration, and the environmental factors on the variation of phytoplankton biomass were carried out at the 23 stations for four seasons in the Shiahae, southwestern coast of Korean Peninsula from February to October in 1995. I made an analysis on biological factor as chlorophyll a concentration as well as environmental factors such as water temperature, salinity and nutrients; ammonia, nitrite, nitrate, dissolved inorganic nitrogen, phosphate, N/P ratio, silicate and Si/P ratio. The waters in the Shiahae were not stratified due to the tidal mixing and high velocity of tidal current. And the high productivity in photic layer were supported by high nutrients concentration from freshwater on lands and bottom waters The low depth of transparency in the Shiahae had a bad influence upon primary production and marine biology. In Shiahae had a sufficient nutrients for primary production during a year. Especially dissolved inorganic nitrogen and silicate were high, the other side, phosphate was low. The source of nutrients in summer and silicate supply depend on input of freshwater from lands, the other side, dissolved inorganic nitrogen and phosphate were depend on rather supplied from bottom layer by the mixing and input of seawater from outside than input of freshwater from lands. Phosphate seemed to become a limiting nutrient for the primary production at all area of Shiahae in winter and at the northern parts in other seasons. However, dissolved inorganic nitrogen seemed to do it at the southern parts in other seasons except winter. Silicate didn't become a limiting nutrient for diatoms in Shiahae. Phytoplankton biomass as measured by chlorophyll a concentration was very high all the year round, it was controlled by the combination of the several environmental factors, especially of nitrogen, phosphorus and the physical factors such as light intensity. [Spatio-temporal distribution, Seasonal fluctuation, Nnutrients, Chlorophyll a, Environmental factors, Nutrient source, Limiting Nutrient, Light, Shiahae] .

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.4
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• pp.389-396
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• 1994

Poor establishment of white dover (Trifolium repens L.) into grass-dominant pastures has been limited its availability. The experiment was done to clarify the effects of timing of initial defoliation, defoliation frequency on the regrowth and nodule formation of the clover cultivars during 28-day regrowing period. Individual plants of cv. Regal, Louisiana S-1 (La. S-1), Grasslands Huia (Huia) and Aberystwyth S184 (S184) were grown in containers until grown to unifoliolate, 1, 2, 4, or 8 trifoliolate stage, and then clipped to 1cm in height every 7 or 28 day for 28 days. To measure the effects, plants were sampled immediately after final harvest, and 1, 3, 7, 14 and 28 days after the harvest. Shoot, root dry weight and biomass were reduced with earlier, more frequent defoliation or shorter regrowing period. In frequent defoliation shoot dry weight and biomass were increased with delayed initial defoliation while in less frequent defoliation steeply done when initial defoliation was delayed to 4 trifoliolate stage. Shoot /Root ratio inclined with more frequent defoliation or lengthened regrowing period, and was greater in initial defoliation of unifoliolate to 2 trifoliolate than the others. Although nodules no. per plant declined with earlier or more frequent defoliation, the effect disappeared to some extent after 14-day regrowth. In comparison with the others, Regal had the highest shoot. dry weight and biomass to 2 trifoliolate stage while S184 did the most nodules regardless of defoliation timing. On 7-day after last defoliation nodule formation of Regal, Huia and S184 but on 28-day after last defoliation that of La. S-1, Huia and S184 was positively correlated to shoot and root dry weights upto 2 trifoliolate stage. On the former day, however, that was negatively correlated to Shoot /Root ratio upto 1 trifoliolate stage although on the latter day it was not, meaning that in addition to more frequent defoliation earlier defoliation was harmful in nodule formation of white clover.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.20 no.1
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• pp.43-52
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• 2015

The growth and population dynamics of eelgrass (Zostera marina) due to changes in sediment composition were examined in the lower intertidal zone of the Seomjin Estuary, Korea. We surveyed environmental factors such as water temperature, underwater irradiance, main types and organic content of sediment, tidal exposure, and nutrient concentrations in the water column and sediment pore water, in relation to the shoot density, biomass, morphological characteristics, and growth of Z. marina inhabiting lower intertidal zones. The survey was conducted monthly from May to December of 2004 and 2009. The water temperature showed obvious seasonal trends in both study years. Underwater irradiance was significantly higher in 2009 than in 2004. Tidal exposure was not significantly different between 2004 and 2009. The sediment was muddy-sand in 2004 but became sandy and with a significantly lower organic content in 2009. Water column $NH_4{^+}$ concentrations were significantly higher in 2004 than in 2009. Sediment pore water $NO_3{^-}+NO_2{^-}$ concentrations were significantly higher in 2009 than in 2004. Other nutrient concentrations did not differ significantly between 2004 and 2009. Morphological characteristics, including eelgrass length and leaf width were significantly lower in 2009 than in 2004. Eelgrass shoot height, leaf length, and sheath length showed typical seasonal patterns, increasing in early summer and decreasing in autumn, in both years. Vegetative shoot density was not significantly different between 2004 and 2009, while the biomass of individual plant parts and the total biomass were significantly lower in 2009. Eelgrass leaf productivity did not differ between years, but leaf turnover time was significantly shorter in 2009 than in 2004. Eelgrass downsizing and decreased turnover time in 2009 compared to 2004 indicate more effective adaptations to the stress of long-term changes in sediment composition. Overall, results suggest that changes in sediment composition can be a limiting factor for seagrass growth in the intertidal zone.

• Journal of Wetlands Research
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• v.15 no.3
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• pp.347-356
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• 2013

To provide the information for the wetland management considering the water treatment ability of macrophytes, the growth characteristics and primary production by reed (Phragmites australis) and cattail (Typha angustifolia), and the decomposition rate of organic matter produced were investigated in two sub-wetlands (Banweol and Donhwa wetlands) of the Sihwa Constructed Wetland (CW) with different chemistry of inflows. The shoot height of P. australis and Typha angustifolia began to increase in March, and reached its peaks in July and August (340cm and 320cm, respectively). The shoot density of P. australis ranging $100{\sim}170EA/m^2$ was higher than that of T. angustifolia (max. $78EA/m^2$). Standing biomass of P. australis ranged from $1,350{\sim}1,980gDM/m^2$, with maximal biomass in Banwol Upper Wetland. And it was larger in upper wetlands than lower wetlands. On the other hand standing biomass of T. angustifolia ($1,940gDM/m^2$) was similar to that of P. australis in Banwol Upper Wetland. Primary productivity of P. australis was in the order of Banwol Upper Wetland ($2,050gDM/m^2/yr$) > Donghwa Lower Wetland ($1,840gDM/m^2/yr$) > Banwol Lowerr Wetland ($1,570gDM/m^2/yr$) ${\fallingdotseq}$ Donghwa Lower Wetland ($1,540gDM/m^2/yr$), and that of T. angustifolia ($2,210gDM/m^2/yr$) was higher than P. australis. Annual production of organic matter produced by P. australis and T. angustifolia was 845 ton DM/yr (423 ton C/yr), and about 90% was comprised of that by P. australis. From the litter decomposition rate (k) (P. australis: leaf 0.0062/day, stem 0.0018/day; T. angustifolia: leaf 0.0031/day, stem 0.0018/day), leaf was rapid degraded compare to stem in both P. australis and T. angustifolia. The litter decomposition rate of leaf was two times rapid P. australis than T. angustifolia, whereas that of stem was same in both. Annual litter decomposition amount of P. australis than T. angustifolia was 285 ton C/yr(67.3% of organic matter produced by macrophytes), indicating that 32.7% of organic matter produced by macrophytes is accumulated in the Sihwa CW.